Brett Goldenberg

Ethan Rahal

Lab Project Proposal

Calcium Chloride

Part 1:
0.02M and 0.08M Amylase as enzymes both provided in the lab.

Volumes:
Enzyme: 25µL x 30 well= 750µL total
0.02M= 375µL + 125µL (just in case of issues) = 500µL
0.08M= 375µL + 125µL (just in case of issues) = 500µL

Part 2:
Substance: Calcium Chloride
Given that 360µL is needed for each concentration, we will create 500µL to account for any
possible errors.

1.0M:
1.0M CaCl2 is already provided and 925µL is needed in order to create the other dilutions.

0.50M:
425µL of 1M will be diluted with 425µL of water to create 850µL of 0.50M CaCl2.

C1V1 = C2V2
(1.0M)(425µL) = C2(850µL)
C2 = 0.50M

0.25M:
350µL of 0.50M will be diluted with 350µL of water to create 700µL of 0.25M CaCl 2.

C1V1 = C2V2
(0.50M)(350µL) = C2(700µL)
C2 = 0.25M

0.10M:
200µL of 0.25M CaCl2 will be diluted with 300µL of water to create 500µL of 0.10M CaCl2.

C1V1 = C2V2
(0.25M)(200µL) = C2(500µL)
C2 = 0.10M
 Concentration(M) Total Volume to Create(µL) Final(µL)

1.0 925 500

0.5 850 500

0.25 700 500

0.10 500 500

Figure 1. Total created and final volumes of CaCl2 needed

18 1 2 3 4 5 6 7 8 9 10 11 12
standard
curve
wells

40µL 40µL 40µL 40µL 40µL 40µL

buffer buffer buffer buffer buffer buffer
40µL 40 µL 40µL 40µL 40µL 40µL
Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6
amylose amylose amylose amylose amylose amylose

40µL 40µL 40µL 40µL 40µL 40µL

buffer buffer buffer buffer buffer buffer
40µL 40 µL 40µL 40µL 40µL 40µL
Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6
amylose amylose amylose amylose amylose amylose

40µL 40µL 40µL 40µL 40µL 40µL

buffer buffer buffer buffer buffer buffer
40µL 40µL 40µL 40µL 40µL 40µL
Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6
amylose amylose amylose amylose amylose amylose

25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 50µL 50µL 50µL
amylose amylose amylose amylose amylose amylose amylose amylose amylose buffer buffer buffer
30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL
H2O H2O H2O H2O H2O H2O H2O H2O H2O water water water
25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL
buffer buffer buffer 0.02M 0.02M 0.02M 0.08M 0.08M 0.08M
amylase amylase amylase amylase amylase amylase

25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 50µL 50µL 50µL
amylose amylose amylose amylose amylose amylose amylose amylose amylose buffer buffer buffer
30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL
1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaCl2 1MCaC2l
25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL
buffer buffer buffer 0.02M 0.02M 0.02M 0.08M 0.08M 0.08M
amylase amylase amylase amylase amylase amylase
 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 50µL 50µL 50µL
amylose amylose amylose amylose amylose amylose amylose amylose amylose buffer buffer buffer
30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL
0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa 0.5MCa
Cl Cl Cl Cl Cl Cl Cl Cl Cl Cl Cl Cl
25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL
buffer buffer buffer 0.02M 0.02M 0.02M 0.08M 0.08M 0.08M
amylase amylase amylase amylase amylase amylase

25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 50µL 50µL 50µL
amylose amylose amylose amylose amylose amylose amylose amylose amylose buffer buffer buffer
30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL
0.25MCa 0.25MCa 0.25MCa 0.25MC 0.25MCa 0.25MCa 0.25MCa 0.25MC 0.25MCa 0.25MCa 0.25MCa 0.25MCa
Cl Cl Cl aCl Cl Cl Cl aCl Cl Cl Cl Cl
25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL
buffer buffer buffer 0.02M 0.02M 0.02M 0.08M 0.08M 0.08M
amylase amylase amylase amylase amylase amylase

25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 50µL 50µL 50µL
amylose amylose amylose amylose amylose amylose amylose amylose amylose buffer buffer buffer
30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL 30µL
0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa 0.1MCa
Cl Cl Cl Cl Cl Cl Cl Cl Cl Cl Cl Cl
25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL 25µL
buffer buffer buffer 0.02M 0.02M 0.02M 0.08M 0.08M 0.08M
amylase amylase amylase amylase amylase amylase

Figure 2. 96-well plate compositions