Princess Oto

BISC110P-F21 Homework-9 Instruction Page

Due Friday, Dec. 9 by 9:55am (10 points, graded)
Instructions
• Collaboration
Homework-9 is open book (books, notes, class materials, and internet searching are permitted).
You may collaborate with BISC110P classmates to discuss the homework questions, but each
student must write their answers independently and individually. If you collaborate with
classmates, please list their names on the submitted assignment.

• Format
o Do not include this instruction page in your submitted responses.
o Please type your homework answers in a distinct font color.
o Please save your completed homework in PDF or .docx formats, then upload to Sakai

• Bulleted list
Where applicable, please organize your answer using a bulleted list to highlight the key points
that show the logic of your response.

p. 1
ATP Energetics and Coupling (3 points)

1. (2 points) You are interested in the process of ATP hydrolysis.

a. ATP hydrolysis is (Please check all that apply):

v -
___ Endergonic ___ Exergonic ___ -DG ___ +DG

b. You decide to hydrolyze ATP in a laboratory test tube. You measure the heat produced
during this reaction, and determine that about 3 times as much heat is released than when the
same amount of ATP is hydrolyzed in a cell. Explain these findings, given the laws of
thermodynamics we discussed in lecture 20 and the concept of thermodynamic coupling.

Based the
on 2ndhamof if
thermodynamics, ATP isbeinghydrolyte
in
or

hycolysis of ATP. Where as in a well, since of's a

living well, ATP
hydrolysis
can
spontaneously
occur
without needing energy. Hence the reaction becomes

favourable

2. (1 point) Describe (in 1-2 sentences) a reaction or event that occurs in a eukaryotic cell coupled to
ATP hydrolysis that was not discussed in lecture 22.

I think
photosynthe (1).

Glycolysis and the Citric Acid Cycle (3 points)

For each of the following questions, indicate the best answer or answers.

1. Glycolysis results in the partial oxidation of glucose to pyruvate. This means that:

a. the electron carriers donate electrons to proteins in the mitochondrial matrix that
in turn produce ATP.
⑤b. glucose is broken down partially to ATP in the cytoplasm.
c. glucose combines with oxygen in the cytoplasm to get partially oxidized.
d. glycolysis consists only of exergonic reactions so that ATP can be made from the
release of energy.
⑧ e. in the process of the conversion of glucose to pyruvate, some potential energy is
transferred to NADH and ATP.

p. 2
 2. During which stages of cellular respiration is carbon dioxide released? Select all that
apply.

a. pyruvate oxidation
⑧b. the citric acid cycle
c. electron transport chain
d. glycolysis
e. oxidative phosphorylation

3. What glycolysis products are transported into the mitochondria? Select all that apply.

a. NADH
b.
⑨ Pyruvate
c. ATP
d. glucose

Cellular Respiration (4 points)

1. (1 point) Label the diagram below with the following terms.
• NADH
• Mitochondrial matrix
• Intermembrane space

Mitochondrial
Matax

MAR
intermembrane
space
Intermembrane
The proton concentration is highest in which region of the mitochondria? ______________ space
⑧⑫
The ultimate electron acceptor in cellular respiration is _____________________________

p. 3
2. (3 points) FCCP is an “ionophore”, which is a chemical that binds to protons and allows them to
travel across membranes from high to low concentration. You are growing liver cells in culture in the
presence of glucose, and you add FCCP to these cells.

a. What is the predicted effect of adding FCCP on the proton gradient in mitochondria? Briefly
explain your answer.
v
_____ Proton gradient will be unaffected
_____ Proton gradient will increase
_____ Proton gradient will dissipate.

b. What is the predicted effect of adding FCCP on ATP synthesis? Briefly explain your answer.
_____ ATP synthesis will be unaffected
- ATP synthesis will increase
_____
_____ ATP synthesis will decrease.

c. What is the predicted effect of adding FCCP on NADH oxidation? Briefly explain your
answer.
_____ NADH oxidation will be unaffected
v NADH oxidation will increase
_____
_____ NADH oxidation will decrease.

p. 4