Atividade - Abstract

Isabel Bastida Medeiros

Metodologia Científica

ARTIGO: Selenium bioaccessibility in rice grains biofortified via soil or foliar

application of inorganic Se.

Autoria: Alexandre Boari de Lima, Thais de Andrade Vilalta, Josimar Henrique de

Lima Lessa, Guilherme Lopes, Luiz Roberto Guimarães Guilherme, Marcelo Braga
Bueno Guerra.

Revista: Journal of Food Composition and Analysis (Fator de Impacto: 4,556; Qualis
(novo): A2; Qualis - Química (2016): B1.

Abstract: Selenium (Se) is a micronutrient for humans, and its deficiency is related
to various diseases. Recent studies have proposed strategies to increase Se intake,
especially using selenium biofortification in staple foods. However, few investigations
shed light on the chemical species and the bioavailability of Se in cereal grains,
including rice, one of the most important staple foods worldwide. This study aimed to
determine the bioaccessible fractions of Se in rice grains biofortified with inorganic
Se species using an in vitro sequential extraction method to simulate human
digestion. Samples and extracts were submitted to microwave-assisted acid
digestion, and total/bioaccessible Se levels were determined by DRC-ICP-MS.
Accurate Se quantification was achieved using matrix-matching calibration. Rice
biofortification via foliar application increased Se levels in the bioaccessible fraction
more effectively than soil applications. Bioaccessible Se represented, on average, 61
± 5 % of the total levels. Of the total Se content in rice, 44 ± 4 % was extracted in the
gastric step, which is where most protein-bound bioaccessible selenium is degraded.
Biofortification with inorganic forms of Se effectively increases the bioaccessible
content of this element in food. Still, the amount to be applied must be corrected
based on the bioaccessible fraction of the nutrient in rice grains.

Elementos do Abstract:

Contextualização: Selenium (Se) is a micronutrient for humans, and its deficiency is

related to various diseases. Recent studies have proposed strategies to increase Se
intake, especially using selenium biofortification in staple foods;

Gap: However, few investigations shed light on the chemical species and the
bioavailability of Se in cereal grains, including rice, one of the most important staple
foods worldwide.
Metodologia: This study aimed to determine the bioaccessible fractions of Se in rice
grains biofortified with inorganic Se species using an in vitro sequential extraction
method to simulate human digestion. Samples and extracts were submitted to
microwave-assisted acid digestion, and total/bioaccessible Se levels were
determined by DRC-ICP-MS.

Resultados: Accurate Se quantification was achieved using matrix-matching

calibration. Rice biofortification via foliar application increased Se levels in the
bioaccessible fraction more effectively than soil applications. Bioaccessible Se
represented, on average, 61 ± 5 % of the total levels. Of the total Se content in rice,
44 ± 4 % was extracted in the gastric step, which is where most protein-bound
bioaccessible selenium is degraded.

Consideração final: Biofortification with inorganic forms of Se effectively increases

the bioaccessible content of this element in food. Still, the amount to be applied must
be corrected based on the bioaccessible fraction of the nutrient in rice grains.