ISM SPLIT PRECISION REPORT

ACQUI SI T I ON I NF ORMAT I ON

System Name: 2695_2998_ISM_QDa72 Acquired By: ryan

Processing Method: ISM Caffeine PM Sample Kit 2 Lot Number: 1245
Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Sample Kit 2 Exp. Date: 6/27/2007 2:52:34 PM EDT
Sample Set Id: 4810 Column Serial Number: 017139167157 42
Sample Set Name: Template_ISM Split Prec SSM Result Set Id: 4816
Sample Set Start Date: 12/23/2014 9:30:47 AM EST

TEST DESCRI PTI ON

In the ISM Split Precision Test , a blank injection followed by six (6) on column injections of Caffeine
are made into a flow of 0.6 mL/min mobile phase with a split flow of 0.5 mL/min provided by the ISM.

The test passes if the Average ISM Retention Time Offset is less than or equal to 5.0 seconds and
the standard deviation for the ISM retention time offset is less than or equal to 1.0 seconds.

The test passes if the Caffeine sample peaks of the Optical Detector have a %RSD less than or equal
to 1.0% for Area, a %RSD less than or equal to 1.5% for Height and a Standard deviation less than or
equal to 1.0 seconds for Retention Time.

ISM Retention Time Offset Results

RT Offset
ISM Delta RT Avg ISM Delta RT RT Offset Standard Retention Time Offset
ISM Delta RT Spec Standard Division
(sec) Pass/Fail Deviation Spec Standard Deviation
Pass/Fail
1 Delta RT Spec = < or 5.0 sec 4.21 PASS SD RT < or = 1.0sec 0.06 PASS

ISM UV System Precision Results

Retention Retention Time
Area Area Height Height Retention Time
Area (%RSD) Height (%RSD) Time Standard
Spec Pass/Fail Spec Pass/Fail Pass/Fail
Spec Deviation (sec)
1 %RSD < or = 1.0 0.16 PASS %RSD < or = 1.5 0.67 PASS SD RT < or = 1.0sec 0.06 PASS

Printed 2:18:26 PM US/Eastern 1/12/2015 Page: 1 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 ISM SPLIT PRECISION REPORT
ACQUI SI TI ON I NFORMAT I ON

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4810

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4816

Printed 2:18:26 PM US/Eastern 1/12/2015 Page: 2 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 ISM SPLIT PRECISION REPORT
ACQUI SI TI ON I NFORMAT I ON

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4810

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4816

UV Detector Caffeine Overlaid Chromatograms

0.80

0.60
Caffeine - 0.591

0.40
AU

0.20

0.00
0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00
Minutes
Sample Name: Caffeine; Injection: 1
Sample Name: Caffeine; Injection: 2
Sample Name: Caffeine; Injection: 3
Sample Name: Caffeine; Injection: 4
Sample Name: Caffeine; Injection: 5
Sample Name: Caffeine; Injection: 6

UV Detector Caffeine Chromatographic Peak Results

Retention Time Height Delta RT of Injection
Injection Name Area Peak Type Result Id
(min) (µV) (sec)
1 1 Caffeine 0.591 2779658 779572 Found 4847 4.2138
2 2 Caffeine 0.588 2784215 772924 Found 4849 4.1349
3 3 Caffeine 0.590 2773588 774509 Found 4851 4.2833
4 4 Caffeine 0.590 2781116 765624 Found 4853 4.2650
5 5 Caffeine 0.589 2785875 767036 Found 4855 4.1364
6 6 Caffeine 0.589 2784489 770171 Found 4857 4.2244

MS Detector Caffeine Peak Results

Retention Time Delta RT of Injection
Injection Name Peak Type Result Id
(min) (sec)
1 1 Caffeine 0.521 Found 4846 4.2138
2 2 Caffeine 0.519 Found 4848 4.1349
3 3 Caffeine 0.519 Found 4850 4.2833
4 4 Caffeine 0.519 Found 4852 4.2650
5 5 Caffeine 0.520 Found 4854 4.1364
6 6 Caffeine 0.519 Found 4856 4.2244

Printed 2:18:26 PM US/Eastern 1/12/2015 Page: 3 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 ISM SPLIT PRECISION REPORT
ACQUI SI TI ON I NFORMAT I ON

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4810

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4816
Injection Delta RT
Average
Name Delta RT Average (sec)
1 Caffeine 4.2096

Printed 2:18:26 PM US/Eastern 1/12/2015 Page: 4 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00