Wavelength (nm) Absorbance of red dye Absorbance of DCPIP

360 0.128 0.12

380 0.111 0.076
400 0.112 0.05
420 0.122 0.04
440 0.15 0.133
460 0.21 0.051
480 0.314 0.075
500 0.419 0.113
520 0.46 0.162
540 0.413 0.22
560 0.295 0.281
580 0.146 0.338
600 0.052 0.359

Fig. 4. Absorbtion at varying waveleng

Absorbance of red dye Polynomial (Absorban
Absorbance of DCPIP Polynomial (Absorban
0.5
0.45
0.4
0.35
0.3
Absorbtion

0.25
0.2
0.15
0.1
0.05
0
360 410 460 510

Wavelength (nm)
btion at varying wavelengths
dye Polynomial (Absorbance of red dye)
IP Polynomial (Absorbance of DCPIP)

460 510 560

Wavelength (nm)
 Wavelength (nm) Absorbance of DCPIP Transmittance (T)
360 0.12 1
380 0.076 0.9
400 0.05 0.8
420 0.04 0.7
440 0.133 0.6
460 0.051 0.5
480 0.075 0.4
500 0.113 0.3
520 0.162 0.2
540 0.22 0.1
560 0.281
580 0.338
600 0.359

Fig 1. Absorbance (A) against transmittance

Absorbance (A) Polynomial (Absorbance (A))
1
0.9
0.8
0.7
Absorbance (A)

0.6
0.5
0.4
0.3 Fig. 2. Absorbance (A) again
0.2 transmittance
0.1
1
0 0.9
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.8 0.9 1
0.7
Absorbance (A)

Transmitance
0.6
0.5
0.4
0.3
0.2
0.1
0
1 1.1 1.2 1.3 1.4 1.5
Log Percent Transm
 %T Log(%T) Absorbance (A) Transmittance (T) Absorbance (A)
100 2 0 1 0
90 1.95 0.046 0.9 0.046
80 1.9 0.097 0.8 0.097
70 1.85 0.155 0.7 0.155
60 1.78 0.222 0.6 0.222
50 1.7 0.301 0.5 0.301
40 1.6 0.398 0.4 0.398
30 1.48 0.523 0.3 0.523
20 1.3 0.699 0.2 0.699
10 1 1 0.1 1

Fig. 3. Absorbance at λmax (600nm) at varying

DCPIP concentrations
0.701
f(x) = 0.184 x − 0.0110000000000001
Absorbance at λmax (600nm)

0.601
0.501
0.401
0.301
Fig. 2. Absorbance (A) against log percent 0.201
transmittance 0.101
0.001
0 0.5 1 1.5 2 2.5 3 3.5 4
0.9 1
DCPIP Concentration (M x 10-5)

1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2

Log Percent Transmittance
 Log(%T) DCPIP Concentration (M x 10^-5)
2 4
1.95 3
1.9 2
1.85 1
1.78 0
1.7
1.6
1.48
1.3
1

m) at varying

5 3 3.5 4
-5)
Absorbance at λmax (600nm)
0.725
0.55
0.351
0.158
0.001

E = 18400 litres/mole/cm
Time (min) Tube #1 Tube #2 Tube #3 Tube #4
0 0 0 0 0
0.5 0.137 0.095 0.06 0.048
1 0.192 0.137 0.104 0.076
1.5 0.231 0.173 0.137 0.097
2 0.265 0.202 0.162 0.118

0.25

0.2
Cumulative change in absorbance (ΔA600)

0.15

0.1

0.05

0
0 0.5 1

Time (min)
Fig. 5: Cumulative change in absorbance at λmax (600nm) of
 Time (min) Tube #
Tube #5 Tube #6 6
0 0 5
0.018 0.007 4
0.045 0.007 3
0.057 0.007 2
0.065 0.007 1

Rate of DCPIP reduction (mol/min x 10-8)

4

2
Tube #1
Tube #2
Tube #3
1
Tube #4
Tube #5

0
0 1

Fig. 6: Rate of DCPIP reductio

(Na2S2O4
1.5 2

at λmax (600nm) of six tubes of vs time

 Initial concentration of sodium hydrosulfite (moles/L x 10-5)
0
1
2
3
4
5

0
0 1 2 3 4 5

Initial concentration of sodium hydrosulfite (moles/L x 10-5)

. 6: Rate of DCPIP reduction and initial concentration of reducing agent

(Na2S2O4) present in each reaction
 Rate of DCPIP reduction (mol/min x 10-8)
0.19
1.23
2.07
2.84
3.75
5.27

3 4 5

sulfite (moles/L x 10-5)

of reducing agent
DCPIP concentration (moles/L x 10-5)
Distance from lamp (cm) Photon fluence rate (µmoles photons/m2/sec)
5 420
10 155
15 82
20 52
25 32
30 22
35 17
40 13

Fig. 6. Photon fluence rate (µmoles photons/m

distances
Photon fluence rate (µmoles photons/

420

360

300
m2/sec)

240

180

120

60

0
5 10 15 20 25

Distance from lamp (cm)

 DCPIP Concentration (M x 10^-5)
4
3
2
1
0

Fig. 7. Absorption at λmax (600nm) at va

1

f(x) = 0.2262 x + 0.00200000000000011

0.8

Absorption at λmax (600nm)

0.6

0.4

0.2

0
0 1 2
DCPIP Concentration (M

nce rate (µmoles photons/m2/sec) at varying

distances

15 20 25 30 35 40

Distance from lamp (cm)

 Absorption at λmax (600nm)
0.9
0.69
0.454
0.228
0

on at λmax (600nm) at varying DCPIP concentration

00000000000011

1 2 3 4
DCPIP Concentration (M x 10^-5)
Photon fluence rate (µmoles photons/m2/sec)
420
155
82
52
32
22
17
13
Moles of DCPIP reduced/min/µg of chlorophyll (n x 10-8)
2.73
2.58
2.22
1.61
1.28
1.17
1.04
0.756

Fig. 8. Moles of DCPIP reduced/min/µg of chlorophyll (n

x 10-8)
3
Moles of DCPIP reduced/min/µg of chlorophyll (n x 10-8)

2.5

1.5

0.5
0 60 120 180 240 300 360

Photon fluence rate (µmoles photons/m2/sec)

hlorophyll (n

300 360 420

Concentration of DMCU (mol/L) (M x 10^-7)
0
0.688
1.38
2.06
2.75
3.44
Rate of reduction of DCPIP (moles photoreduced/min) (10^-10)
8.77
6.46
5.72
2.29
1.72
0.686

Fi g. 9. Rate of reducti on of DCPIP (

duced/mi n 10^-10) agai nst concent
9

8
Rate of reduction of DCPIP (moles photoreduced/min) (10^-10)

0
0 0.688 1.38 2.06

Concentration of DMCU (mol/L) (M x 10^-7)

cti on of DCPIP (mol es photore -
agai nst concentrati on of DCM U

1.38 2.06 2.75 3.44

tration of DMCU (mol/L) (M x 10^-7)

#cells/mL x E6 A400
12.6 0.399
7.56 0.251
5.04 0.17
2.52 0.078
0 0

Fig. 10. No. of cells/ml against absorbance

0.45
0.4
f(x) = 0.0319176319176319 x + 0.00264864864864867
0.35
0.3
Absorbance

0.25
0.2
0.15
0.1
0.05
0
0 2 4 6 8 10 12
#cells/mL x E6