Fig 1.

Absorbance (A) against transmittance

Absorbance (A) Poly. (Absorbance (A))

1
0.9
0.8
0.7
Absorbance (A)

0.6
0.5
0.4
0.3
0.2
0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
Transmitance

Fig. 2. Absorbance (A) against log percent transmittance

1
0.9
0.8
0.7
Absorbance (A)

0.6
0.5
0.4
0.3
0.2
0.1
0
1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2
Log Percent Transmittance
 Fig. 3. Absorbance at λmax (600nm) at varying DCPIP
concentrations
0.701
Absorbance at λmax (600nm)

0.601 y = 0.184x - 0.011

0.501
0.401
0.301
0.201
0.101
0.001
0 0.5 1 1.5 2 2.5 3 3.5 4
DCPIP Concentration (M x 10-5)

Fig. 4. Absorbtion at varying wavelengths

Absorbance of red dye Absorbance of DCPIP
Poly. (Absorbance of red dye) Poly. (Absorbance of DCPIP)
0.5
0.45
0.4
0.35
Absorbtion

0.3
0.25
0.2
0.15
0.1
0.05
0
360 410 460 510 560
Wavelength (nm)
Cumulative change in absorbance (ΔA600)
0.25

0.2

0.15

0.1
Tu
be
0.05 #1

0
0 0.5 1 1.5 2
Time (min)

Fig. 5: Cumulative change in absorbance at λmax (600nm) of six

tubes of vs time

5
Rate of DCPIP reduction (mol/min x 10-8)

0
0 1 2 3 4 5
Initial concentration of sodium hydrosulfite (moles/L x 10-5)
Fig. 6 (A): Rate of DCPIP reduction and initial
concentration of reducing agent (Na2S2O4) present in
each reaction
Photon fluence rate (µmoles Fig. 6 (B). Photon fluence rate (µmoles
photons/m2/sec)
photons/m2/sec) at varying distances
420
360
300
240
180
120
60
0
5 10 15 20 25 30 35 40

Distance from lamp (cm)

Fig. 7. Absorption at λmax (600nm) at varying DCPIP

concentration
1
Absorption at λmax (600nm)

0.8

0.6 y = 0.2262x + 0.002

0.4

0.2

0
0 1 2 3 4
DCPIP Concentration (M x 10^-5)
 FIG. 8. Moles of DCPIP reduced/min/µg of
chlorophyll (n x 10-8)
MOLES OF DCPIP REDUCED/MIN/ΜG OF CHLOROPHYLL (N X

2.5

2
10-8)

1.5

0.5
0 60 120 180 240 300 360 420

PHOTON FLUENCE RATE (ΜMOLES PHOTONS/M2/SEC)

Fig. 9. Rate of reduction of DCPIP

(moles photoreduced/min 10^-10)
against concentration of DCMU
Rate of reduction of DCPIP (moles photoreduced/min) (10^-

4
10)

0
0 0.688 1.38 2.06 2.75 3.44

Concentration of DMCU (mol/L) (M x 10^-7)

 Fig. 10. No. of cells/ml against absorbance
0.45

0.4 y = 0.0319x + 0.0026

0.35

0.3
Absorbance

0.25

0.2

0.15

0.1

0.05

0
0 2 4 6 8 10 12
#cells/mL x E6