pETDuet 1

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BamH l

Lac I EcoR l
T7 promoter-1
Sac l
lac O Xba l Pst l
RBS-1 Nco l Sal l
Hind lll
His tag Not l

MCS-1
T7 promoter-2
lac O

pETDuetTM-1 RBS-2

5.4kb Nde l

MCS-2
Bgl l
S tag EcoR V
Kpn l
pBR

Blp l Xho l
T7 terminator
32

i
2

Or
O

i f

1
r

AmpR

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