UNIVERSITY OF CAPE COAST

SCHOOL OF MEDICAL SCIENCES

LABORATORY PRACTICAL QUESTIONS

Post-Lab Questions

1. Why do all living organism require a nitrogen source?

2. Differentiate between the nitrogen-containing compounds utilized and produced during

the following process:

a. Ammonification

b. Nitrifacation

3. Why is it important to check gas production by organism in TSI test?

4. Why is important to also take note of acid production during sugar fermentation? How

does this help us understand the synergistic relationship between two microbes?

5. Differentiate between the following;

a. Respiration and Fermentation

b. Oxidation and Reduction

6. List two difficulties one encounters in trying to differentiate bacteria on the basis of

physiological characteristics.

7. What enzymes are involved in the following reactions. State their significance in

a. Indole production

b. Catalase test

c. Coagulase test

d. Urease test

8. What are the general characteristics of Enterobacteriaceae?