Department of Chemical Engineering,

School of Energy Technology,

Pandit Deendayal Energy University

Course: Biochemical Engineering and Bioenergy Course Code: 20CH318T

Instructor: Dr. Md Aurangzeb Academic Year: 2023-2024

Assignment-Theory (Set 1)
[1]. Explain two chemical theories that describe the functioning of enzymes as biocatalyst?
[2]. Briefly discuss the enzyme specificity hypothesis?
[3]. Develop Michaelis-Menten equation for enzyme kinetics. List down the assumptions used
for the equation. Also, explain the significance of the parameters involved in the equation.
[4]. How do you determine the enzyme kinetic constants from batch reactor data?
[5]. Write a short note on adsorption and covalent method for immobilization of enzymes.
Discuss the advantages of immobilization of enzymes?
[6]. Develop the expression of thermal death kinetics of microorganisms? Following this
development, state a reason of not attaining 100% sterilization?
[7]. Briefly discuss the two method of continuous sterilization?
[8]. Describe the various phases of cell growth in a batch culture, with neat diagram.
[9]. Describe the growth-associated and non-growth-associated product formation in
fermentation process.
[10]. Develop a kinetic equation for the cell growth.
[11]. Develop the expression for the rate of product formation for a non-competitive
inhibitors.
[12]. Develop the expression for the rate of product formation for substrate-inhibited enzyme
reaction using Monod-growth equation. Also, find the expression for substrate and product
rate formation at the product formation rate is maximum.
[13]. Derive the relationship between the true growth and observed yield of biomass growth
over substrate consumption.
[14]. Develop a design equation for biomass growth with time in a batch fermenter.
[15]. Indicate the assumptions and show that the dilution rate is equivalent to specific growth
rate in a continuous stirred tank fermenter.
[16]. Explain graphically the effect of dilution rate on the substrate, biomass and product
during enzyme-catalysed reaction in CSTF.
[17]. With the help of mathematical equation, explain the wash-out condition in CSTF.

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[18]. Develop the expression of optimum dilution rate at which the cell productivity is
maximum in CSTF. At this condition, find the expression for optimal cell concentration
and productivity.
[19]. Mathematically explain the CSTF is more productive than the batch fermenter.
[20]. Develop the biomass and substrate concentration in the CSTF with settler-thickener and
recycle of biomass.
[21]. Briefly, explain the condition of choosing one CSTF and multiple CSTF connected in
series.
[22]. Develop the mathematical equation for two CSTF connected in series, and draw a rough
sketch between the rate of biomass and biomass concentration indicating the slope for each
reactor.

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