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ORGANIZATION OF CnGARIZATIONS OF IMMUNDGLOBUUN GENES ee a toe 8 all ™ IgM ni Beat nniigm + nied Antigen ttimnutalion _—— Beall Se ce OE EM oe —> Fox various ‘solypee of antihoatig KOK Memory call —» Pere various iscty hee Goum Line 3 Aomatic vanistion modal :- Geum Line theot EO aren fe Conthibuled to mares ma § {ems me raat Garctic me ven Rema vvasuiation Ueory — Genome a os eis fhe nO: demas ro. of tg foun Bon poturh o Lasige a : mein one axe germatee dx Somatic calle by ™ Powyes & Bennet _— 2. gore ons borypepite sree 2 rapes traaang Ror or, Gerce encode. a pings 16 hight chain era. feru fon Vario le. S agin one fox teratant xogion Tone garoa's one oo iemmant _ pug un Wea. Deer prom orl teeyorit ‘calls 4 Gexmling showed V BC Gense abiscyone DNA oa loseought chase tagettran inadulb, Multigene oxganigetion st ig geneo - ® ° Ka > Light chain, tauy wrutltigana fanatic, o- ™ foun dine ‘DNA, the rmulligone fanihis contare, teding vagion cltal gene aeqeren® mapanold Mg ron ~ Coding ragions - . Drouing | matin Kew tee — Sans ged 2 brought oThen. Foun. Function TE genes: & — rbight chan Human dh 22 K Light chain > Human che. 2 Henry chau Shuman chy 4 » KS0 Aight chain farniies centain V,T 3 Sena agree WI = toda fon vaniabbh ion Cc > code fon constant Jape ‘ » He chain, ami containg VD, T&C pore t ¢ beaded tide lon ¥ VDT v2 da vebnile im, ty at Jt S' and ave a Amol» . NV fox. cohen aiqras / tndent «peptide exon Ah Li chor though ER. 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CH Coen for tipo Seles 1Cg 0m Light -chain DNA _undwegser veo swarnangemants ® hin Pan ke Ck oo R-cun a) = [eens “he tet al Tx- Cx Reosmnanged ih =: = 1. chain DRA tesla ~ - Je L aaquents Po 3! Pedeneny Tanscnigt — bhi Set arong a Lv Ce Atel een oo pat Sots oye Lenn wenart lyin tamaugh © agement vite primary © Light chain. me, 4 She tnbieno ome samowed ‘ rRNA entous eytoplaom -from. nucleus” © Light Charm MRNA Granoibed & L asapance cloowed in ER. Heavy chain DNA Moartaly cH CS CG iprawayy) eeeeecas. ieee eddeeees. bee se ar saa Seder, “Toco Geiption va Lyps aa” ont Poul 8 6 Rwa I Polyedaniation Brod tplieenge Ly ar cu. ono cs RNA so tien | Temsloten Lyps ¢) typs co pevypopt rm ge es Mechoniim of variable sagion Dvn raarvengonent © Recombinstion wgnal xquance. (R66) DNA raquencer rH Consoiwed bp. in vaeiabie, segi'sn % fending . Those Wnigue sequances ese RSS. flanking ‘each geen tine, VDT gene aagrant . GQrttrrar. TG Gra) ATT TI TQG Tqacac — sNenome Heptomer “Tose Kasam ROE Ona. Mun RSS reboindXh SHB Ome2’ Va hao 2am spacer. Ta oreline 1C- chau c! ams: Ne 1 Tin spoon Aesany chain, 4 "Vi D Te - 8 Gen apace ° Dy > Two {-Cowtprer Signal uantes Shourng eno Siew will ab says a ‘ene. ments axu jeuned secombinase “Two protein lavelved Jn Moromalination dedod HeLombi nation ackivating Gorse BM 1 & Rage “t 3 v(v)s Soosnang : Ye xetemlatnation Alas axa at Fottoust - is 1) Recognistion of R88 bi secombinane eae Jn ohh 2 vances & odjas at tye ge at Kean nt a) Cheay erg athond of DNA RAG, LiRAGe of an “Scon! of tig! pol ling a> RAG 1 & @AGZ the seeaciien hse on Shord, altace the ss oot lining opp. abiord ho tho aiginal vag uance~ hainpptn A Gusting ot haixpin G Serenalt aie Allis fox te of P- agin ouclectidea , ton aiten® fuse nudavtides 4am Te Teaing 2 ‘ ty Singh ond udonuctaaet 3) Addition Of Seg as af Ha cut ends v Deg sagrrants toumnal deoxynudl tidus Te He 6) Reger & Sigains of codung vacaytence tif , Dobetionalljinung ing Unverceivnal fining ibe E> te ty © muclantida, addition “es = —_——— Sie : Fe Say Sct =O %, = 1 Spe SB N-nudaottda addition Spi Cait tay" ata (sie add, Nenuclads sees =e AAQTT ATA agence ATAT Genowahion of Artibadiy Dive © . Multiple geun-—tine Gene aagmant, ; + Combinatorial V-(n)- T Joining « Functional Plani biti a + P- segion mudectida addition +N -7egion nuctectida addition Somatic Rugpes mutation * Comlaimatorual association af Light i “= Multiple. geum-line V,D ¥T gen xeornang ect pea hybudization vtidise hare shourn thaw owe ‘variable ne- Vic, Vi Ot Va - L thw ae muldipl no of IL DH ® Teg eager 5 conbibule aiverify . . <3 Seinng Sr Neulat ne ; gong B00 - (oc0 200 2 D - 13 ° ° TY - 4 4 a Aetna EVOT go0n13 4 = Ext Boonyel2xi 2n3 26 «Foard Justis * ~ : © Pe sagion * + » Ne Xegion Muclesticle * - * Fre mutation + + + 2 SES eset Se cota to aaah gam raan? Peeductive 3 Non- Aliabic ocelutton @ t ibis ae si _tactat GQTGGACGTT....3' sn QGGAaTeeTeee CACAG TG» 3 Veo Rss Pra -B call Coding fein’ Si ° mal fer nt lines Cy, § ) veins Call bie #4 8’ GanTee qaacgTT 3 | MCAETETE CA Agra” Gell Lina, 8) QGATETEGACG TTS" Stactag cAcasTa Scactq Tq CACAgTA 8! GqarectegtqgacqTr SemergTGcAcAaTd # 5 Coth Line # 3 Coll dine HY S*GGATCET TAGACG TT 3 P-nuclectide —Jemistion am thu position at which tu haixpin Je © ga Meads to veluiodfon inthe ‘aequance of code jest . [ Redes re) P- nucleotide tag ht before] cos N - nudlectida. he uel edtdiben ocewts only ny NY nuctestice od ditlon - ding joints -CpR3 = “Tae At naa poretble fon Present ~~ V-D-3 co roo Chain - Diversity ub genexotad oy N- nucheotide. Sematie_H mutation K adittenal SG divewily us generated J Sessonta variable xagion ‘somatic hy portion Gn di nuctostidas wr VI ox VDT wil ve raplatad with allen dive borer atoning aber’ fii a ge. 2b se aves! Dia Wwoprg o oy 5 . (A aie - . a . J eorbigetitroe sys s! Lvbr SStcn Sus - Syda =o a! %, i a Sy rives Phere 2 sacombinalio $82 st ACT ee Em S . Sa re; 8 iy! yt , 5 Aw (Ack Seduced Ss - a “ ._> mectioke a Oe M ‘lon the : ae 208 high than in ether ponas— co hyparoe Mutetions one. untoduced = riot ee =o Usually omubrtitution. of mucleotice takor plate. Comotie Wetikotion axa cluslomd srthin the cate af Vy VE 2 info ovacanl affinity fox orton: Artociion of nent ae ven Finah Aouna of SM be hase eagle combainalo riod Oro yr 9 107 dn human, saul Of gon 6624 H- chain fore & StS Light has perl Stpany one off 8-choun of bed ne, OCCU wn aap arionlore — a ne. conn gia a4, 000. Yho mo. io amudh mona ee of 4 ; Pioritssity « Prudstide 2 N= nuctigtds odlite Glase Switching ® Gupjtth sagions axa Q-Dkb wpsliwae 4 each dagen Cextept ce) » Stosteh ion connitt annul ‘le C&pied Arbor GAGet : tcaaa t % © Susited Combinese, M2 COgn'gg0 thie xapeak z ener out DNA imoutiiew 4 Clans dusit tg . Cyfokines imp. Un Hs brrocekt 2-8, ILA counar ptottehing fom Cu fy Cy, Lb +e Ce.

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