SnapShot:

Directed Differentiation of ESCs and iPSCs

Luis A. Williams, Brandi N. Davis-Dusenbery, and Kevin C. Eggan
HHMI, Harvard University, Cambridge, MA 02138, USA
This SnapShot was previously published in Cell 149, May 25, 2012 ©2012 Elsevier Inc. DOI 10.1016/j.cell.2012.05.015

GERM CELLS E C T O D E R M D E R I V AT I V E S
BMP4
BMP7
BMP8B
Primordial germ Meiotic Haploid + transduction of
cell-like cells + cells + cells Retinal pigment
DAZ genes Epidermis Photoreceptors Otic hair cells Astrocytes Oligodendrocytes Cortical neurons
epithelium
2
Insulin RA Utricle FGF H
Egf stroma SH F t i n o ids
Taurine
PDG Re HNK1+ Neural crest

TF
hCG Culture on coculture 5 + ,
7 stem cells
Sort p

CN
ACTIVIN A Adherent
Spontaneous matrix
culture
Fgf2 FGF8
differentiation Igf1 SHH

Serum
Primordial germ FGF2
Oocyte-like
cells + Follicle-like
structures
cell-like cells
FGF
EGF

SHH
Ectoderm BMP4
Bmp4 Keratinocytes Neural
Sort Scf progenitors GABA neurons DA neurons

de
Lif NOGGIN RA
Transplantation Integrin- β3+ Activin a

KK
SHH

ami
LDN/
Egf
NT

D
into testes Ssea1+ Fgf2
Ascorbic Dorsomorphin
W

otin
acid

Sort C D 7 3 +
BMP4 Spinal motor neurons

Nic
Spermatozoa Primordial germ Epiblast-like cells NODAL LEFTY
cell-like cells ACTIVIN
KSR or adherent culture
SB431542 Cortical layers
BMP4 PA6/MS-5 coculture Optic cup
FGF2 Neural retina (NR )
EGF
VEGF SFEBq culture 3D matrigel Retinal pigment
Sort CXCR+,CD117 high
ACTIVIN A culture epithelium (RPE )
Neuroectoderm

Multipotent Definitive Pluripotent

endodermal F10 endoderm Stem Cells INSULIN, TRANSFERIN Serum Sort CD73+
G
F RA
progenitor cells (ESCs/iPSCs) Selenium
Mesenchymal stem/
TI V I N/
L A-8 OP-9 coculture FGF2, PDGF-BB OP-9 coculture
precursor cells
3-0
TG

AC ODA BMP4 Semisolid

N
Fβ

1
42
VEGF
BMP4
culture Mesenchymo- Dexa, IBXT β-GP
F 3 1 5 SCF angioblast Dexa
HG F4 SB4 TPO FGF2 Insulin
FG F ACTIVIN A Ascorbic Sort
NO

EG FLT-3 Primitive streak Adipogenic cells

Hepatic WNT3A acid NCAM+
GG

mesoderm
BM

progenitors 0 FGF4
F1 Ascorbic acid
IN

NT4
P4

G
F RA CSF, IL-3, IL-6 VEGF,
BMP4, FGF2 NT4, GDF5 TGF β3
SCF, VEGF, BMP4 IL-3, IL-6,
H FLT-3, EPO, TPO IL-11, SCF FOLLISTATIN BMP4, FGF2
Hepatocytes SH i ne Osteogenic
T a m BMP4
P p Semisolid Chondrogenic cells N2
DA clo OP-9 cells
H Cy culture VEGF -Serum
TC TPO coculture DKK DKK
NO Pancreatic
EPO
progenitors Anterior Hematopoietic
Hemangioblast IL-3 WNT WNT
1 endoderm progenitors IL-6 Skeletal
IGF GF e IL-11 VEGF VEGF
myoblasts
H amid EG
F SCF
o tin NOGGIN BMP4 Hindgut BMP4 SCF Monocyte/
Nic endoderm FGF2 TPO OP-9 or MS-5 macrophage
Cardiovascular
WNTs IL-3 coculture
colony-forming cells
3D matrigel FP6 progenitors
culture EPO M-CSF Smooth
β cells RANKL VEGF
SB431542 FGF2
muscle cells

TGF β
Multipotent Erythropoietic Lymphoid
Intestinal lung progenitors cells progenitors Osteoclasts Fibronectin, EGM2
tissue Endothelial cells Cardiomyocytes
E N D O D E R M D E R I V AT I V E S M E S O D E R M D E R I V AT I V E S

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DOCUMENT #28013 | VERSION 1.3.0