LEARNING ACTIVITY SHEETS

GRADE 11/ 12 - BIOLOGY 2

Name: Date: Rating/Score:

DECODING KEY TERMS

Directions: Below are some of the terms associated with Recombinant DNA.
Identify each of the following terms by writing the missing letters in the given box.
Each number has a corresponding letter from the alphabet.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

A B C D E F G H I J K L M N O P Q R S T U V W V Y Z

22 5 3 20 15 18

Carries a small ring of DNA in host cell.

16 12 1 19 13 9 4

A small ring of DNA in bacterium.

18 5 19 20 18 9 3 20 9 15 14 5 14 26 25 13 5 19

Enzymes that cut specific sequences of DNA.

4 14 1 12 9 7 1 19 5

Enzymes that rejoin DNA fragments.

19 20 9 3 11 25 5 14 4 19

DNA fragments with overlapping sides which have unpaired nucleotides at the ends.

20 18 1 14 19 7 5 14 9 3 15 18 7 1 14 9 19 13

An organism created by genetic engineering, wherein one or more foreign genes have
been incorporated into its genome.

7 5 14 5 20 9 3 5 14 7 9 14 5 5 18 9 14 7

The manipulating of genetic material in the laboratory, including isolating, copying and
multiplying genes, recombining genes from different DNA from different organisms or
transferring genes from one species to another, bypassing the reproductive process.

Specific Week: 1 (LAS 1)

Target Competency: Outline the process of genetic engineering DNA (STEM_BIO11/12-IIIa-b-6)
LEARNING ACTIVITY SHEETS
GRADE 11/ 12 - BIOLOGY 2

STEPS IN RECOMBINANT DNA TECHNOLOGY

Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning
allowing genes to be transferred from another biological species easily. Their work
also gave rise to the development of different recombinant proteins with therapeutic
applications like insulin and growth hormone. The former was cloned using
Escherichia coli. coli in 1978. With this breakthrough, the first licensed drug
produced using recombinant DNA technology was human insulin, developed by
Genentech, licensed and marketed by Eli Lilly in 1982. Scientists were able to
identify and isolate the gene fragment or the gene of interest, in this case, the gene
that is responsible for producing insulin. Moreover, they were able to isolate the
bacterial DNA of E. coli. The plasmid and DNA fragment were cut using a restriction
enzyme. This DNA fragment was inserted into the plasmid using a DNA ligase.
When the DNA fragment was then placed into the bacterial DNA, it was then
introduced to the host cell (E. coli) and was then amplified for the selection of
transformed host cells.

Based on what you have read, write the steps in Recombinant DNA.
1. _
2. _
3. _
4. _
5. _

Animal Cell
2 4

DNA
Bacterial cell

Plasmid
Bacterial 1 3 5
chromosome

Illustrated by Divina Gracia Brillantes

Questions:
1. Why are plasmids used as vector for DNA Recombination? What other vectors can
be used?

2. How are Recombinant DNA formed?

3. What is the difference between genetic modification and selective breeding?

Specific Week: 1 (LAS 1)

Target Competency: Outline the process of genetic engineering DNA (STEM_BIO11/12-IIIa-b-6)
LEARNING ACTIVITY SHEETS
GRADE 11/ 12 - BIOLOGY 2

APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY

Directions: Below are some of the Applications of Recombinant DNA Technology in
the DNA of organism. Write the advantages and disadvantages of applying such
application in the DNA of an organism.

1. Development of genetically modified crops that are resistant to pests.

Advantages Disadvantages

2. Drug delivery systems that are based on bacterial or viral host.

Advantages Disadvantages

3. Producing genetically modified organisms to enhance food production.

Advantages Disadvantages

Specific Week: 1 (LAS 1)

Target Competency: Outline the process of genetic engineering DNA (STEM_BIO11/12-IIIa-b-6)
LEARNING ACTIVITY SHEETS
GRADE 11/ 12 - BIOLOGY 2

4. Combining two or more genes in one organism to enhance its traits.

Advantages Disadvantages

5. DNA manipulation of certain species to produce organs for harvesting.

Advantages Disadvantages

Specific Week: 1 (LAS 1)

Target Competency: Discuss the applications of recombinant DNA (STEM_BIO11/12-IIIa-b-7 )