1/27/22, 6:32 PM PhysioEx Exercise 12 Activity 1

PhysioEx Lab Report

Exercise 12: Serological Testing
Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia
Name: Maria Karla M. Balamad
Date: 27 January 2022
Session ID: session-b156bf7c-e8d5-834c-10f3-95dd1b02797d

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Which of the following has an epitope?

You correctly answered: an antigen.

2 Chlamydia trachomatis is

You correctly answered: a bacterium that reproduces inside its host cell.

3 The infectious form of Chlamydia that is spread from person to person is the

You correctly answered: elementary body that stains green in this activity.

4 The goal of the direct fluorescent antibody test is to test for the presence of

You correctly answered: antigen.

Experiment Results
Predict Questions
No Predict Questions
Stop & Think Questions
1 The source of the patient samples is

You correctly answered: epithelial scrapings.

2 What is the name for the specific part of the antigen that the antibody binds to?

You correctly answered: epitope.

3 Drag the first slide (patient A) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall that
elementary bodies stain green), enter the number of elementary bodies.
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1/27/22, 6:32 PM PhysioEx Exercise 12 Activity 1

You answered: 0 elementary bodies.

4 Drag the next slide (patient B) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall that
elementary bodies stain green), enter the number of elementary bodies.

You answered: 15 elementary bodies.

5 Drag the next slide (patient C) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall that
elementary bodies stain green), enter the number of elementary bodies.

You answered: 1 elementary bodies.

6 Drag the next slide (positive control) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall that
elementary bodies stain green), enter the number of elementary bodies.

You answered: 19 elementary bodies.

7 Drag the last slide (negative control) to the fluorescent microscope.

Count the number of elementary bodies you see through the microscope (recall that
elementary bodies stain green), enter the number of elementary bodies.

You answered: 0 elementary bodies.

Experiment Data

Sample Number of Elementary Chlamydia Result

Bodies
Patient A 0 -
Patient B 15 +
Patient C 1 +
Positive Control 19 +
Negative Control 0 -

Post-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

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1/27/22, 6:32 PM PhysioEx Exercise 12 Activity 1

1 In the serology test just performed, the fluorescence is found on the

You correctly answered: antibody.

2 What would most likely happen if you forgot to fix the samples to the slide?

You correctly answered: They would all test negative because the washing steps would
remove the samples.

3 Which sample showed some residual nonspecific binding after the washing steps?

You correctly answered: patient C.

4 Which patient samples contained the Chlamydia-specific antigen?

You correctly answered: patient B.

5 How is chlamydia disease transmission prevented?

You correctly answered: abstinence or faithful, mutual monogamy.

Review Sheet Results

1 Describe the importance of the washing steps in the direct antibody fluorescence test.

Your answer:

The washing steps in the direct antibody fluorescence test is important because it removes
any non-specific binding of antigens and antibodies.

2 Explain where the epitope (antigenic determinant) is located.

Your answer:

Epitopes are located in an antigen where the antibody can bind to it.

3 Describe how a positive result is detected in this serological test.

Your answer:

To test positive for a serological test, there must be ten or more fluorescent antibodies must
be found within 5 mm.

4 How would the results be affected if a negative control gave a positive result?

Your answer:

If a negative control gave a positive result then void the results of the whole experiment
because the control was contaminated.

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