Name _Class_ Date Criterion C

King_7.1_3/7/23

An investigation on the rate of reaction of amylase on starch at different

temperatures

Research question:

Do temperature changes affect the rate of reaction of amylase on starch?

Hypothesis:

If the temperature of the setup increases, the rate of reaction increases; this is because
more energy is provided for the reactants to facilitate the collision chances.

Results
Observation:
At 25°C, brown solution is observed in all the trials. In 25 celcius also trial 3 has blue-black
complex at the bottom of the test tube. And the average of the colour is brown solution.

At 35°C, it only contains brown solution all in the trials test tube. And the average of the
colour was brown solution.

At 45°C, it has completely different colors in the 3 trials, in the first trial the colour was
brown solution, blue-black complex. Trial 2 it has brown solution inside of the test tube, trial
3 it has yellow solution inside of the test tube. And the average colour is brown solution.

At 55°C, all the trial has the same colour inside the test tube, the average of the colour is
brown solution.

At 65°C, it also has the same type of colours inside the test tubes and they are blue-black
complex at the bottom, yellow solution and the average of the colour are blue-black
complex at the bottom, yellow solution.

Raw data and processed data

Table 1.

Temperature of
setup (°C) Trial 1

Observation Trial 2

Average
Trial 3

Brown solution, blue-

25 Brown solution Brown solution black complex at the Brown solution
bottom
35 Brown solution Brown solution Brown solution Brown solution
Brown solution, Blue-
45 Brown solution Yellow solution Brown solution
black complex
Brown solution, a Brown solution, a Brown solution, a
Brown solution. a little complex
55 little complex at the little complex at the little complex at the
at the bottom
bottom bottom bottom
Blue-black complex at Blue-black complex at Blue-black complex at
Blue-black complex at the
65 the bottom, yellow the bottom, yellow the bottom, yellow
bottom, yellow solution
solution solution solution

Formula:

𝑆𝑡𝑎𝑟𝑐h + 𝑤𝑎𝑡𝑒𝑟 → 𝑚𝑎𝑙𝑡𝑜𝑠𝑒

with the help of enzyme catalase as the catalyst. Amy

Graph

Iodine test
Temperature of setup (°C) Average
Trial 1 Trial 2 Trial 3
25 -ve -ve +ve -ve
35 -ve -ve -ve -ve
45 +ve -ve -ve -ve
55 +ve +ve +ve +ve
65 +ve +ve +ve +ve

Name _Class_ Date Criterion C

Analysis and Conclusion Patterns:

For the setups with temperatures below 45°C, the brown solution of the experimental
results means that iodine does not react with starch, and is diluted in the test tubes so that
only the brown color of the iodine solution is expressed. Therefore, the negative result of
the iodine tests means that starch is not present in the test tubes. The starch is likely broken
down by the amylase and remains colorless, so that only the diluted brown color of the
iodine solution is observed. The amylase reacts with the starch, except for only two trials at
25°C and 45°C respectively. However, the blue-black complexes formed in the two trials are
small, compared with those formed in the setup with the highest temperature at 65°C.
(Libretexts, 2020)

On the other hand, blue-black complexes are observed at the bottom of the test tubes for
the setup with temperature at 55°C and 65°C . This means that the iodine tests show
positive results and starch must be present in the test tubes. All three trials at 55°C and 65°C
consistently show the same results. The amylase in the setups does not work as expected to
break down the starch, leaving starch available to be detected by the iodine solution. The
high temperature of these setups likely denatures the enzyme amylase and the reaction
cannot be catalyzed rapidly enough to break down the starch within three minutes.
(Libretexts, 2020) Some iodine in the solution reacts with the starch, so that the solution
color becomes paler and yellow.

Evaluating the Hypothesis:

I predicted that if the temperatures increase the rate of reaction increases. And my data did not
support my prediction because if we adjust the temperature to 25 but it has different colours in the
test tube for example trial 1 Brown solution, Brown solution, Brown solution blue-black complex at
the bottom.

Evaluating the method:

Because if we adjust the temperature to 25 but it has different colours in the test tube for example
trial 1 Brown solution, Brown solution, Brown solution blue-black complex at the bottom and they
have different types of results in temperature 25 celcuis

Suggesting improvements:

We can improve on dropping the starch, amylase and iodine has the same drop amount. Because if
we don’t drop the same amount of starch, amylase and iodine we could have some different datas.
More aware of dropping the amylase, starch, and iodine solution to the floor. Because if we dropped
the materials of the experiment that could be worse or even step on it that we didn't know either .
We need to hold the test tube tight enough in case the test tube falls inside the water bath could
cause the water.

Works Cited
 Libretexts. “10.8: The Effect of Temperature on Enzyme Kinetics.” Chemistry LibreTexts,
Libretexts, 1 Sept. 2020,
https://chem.libretexts.org/Bookshelves/Physical_and_Theoretical_Chemistry_Textbook_
Maps/Map%3A_Physical_Chemistry_for_the_Biosciences_(Chang)/10%3A_Enzyme_Kineti
cs/10.08%3A_The_Effect_of_Temperature_on_Enzyme_Kinetics.