DILUTOR LINEARITY & ACCURACY REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 050 Dilutor Lin Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Acc PM Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Lin Acc Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11576 DB ID:
Result 847558692
Set Id 11600
Sample Set Name: Dilutor Lin Sample Set Start Date: 11/4/2010 6:12:41 PM

The Dilutor Linearity qualification makes injections at varying dilution factors of one concentration and
creates a calibration curve of Peak Area vs. Amount.

The qualification passes if the Linearity R^2 value is > or = 0.9990 and the Accuracy absolute value of
the X-intercept is < or = 0.2uL.

Calculation
The Dilutor Accuracy is calculated using the following formula: |Y intercept/Slope|

Caffeine Dilutor Linearity & Accuracy Results Table

Dilutor
Linearity R^2 Linearity R^2 Dilutor Accuracy Dilutor
Linearity R^2 Accuracy
Specification Pass Fail Specification Accuracy
Pass Fail
1 R^2 > or = 0.9990 0.9997 PASS < or = 0.2 ul 0.0 PASS

Printed 7:55:28 AM US/Eastern 11/17/2010 Page: 1 of 3

 DILUTOR LINEARITY & ACCURACY REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 050 Dilutor Lin Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Acc PM Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Lin Acc Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11576 DB ID:
Result 847558692
Set Id 11600
Sample Set Name: Dilutor Lin Sample Set Start Date: 11/4/2010 6:12:41 PM

Overlay of Caffeine Peak Chromatograms

2.00

1.50
AU

1.00

0.50

0.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20
Minutes
Dilutor Linearity Results Table

Peak: Caffeine
Area
Vial SampleName X Value Level Channel Id Result Id
(µV*sec)
1 7 Dilutor Lin_20 0.05 205086 1 11599 11632
2 7 Dilutor Lin_10 0.10 407223 2 11607 11633
3 7 Dilutor Lin_5 0.20 804750 3 11614 11634
4 7 Dilutor Lin_2 0.50 1994593 4 11621 11635
5 7 Dilutor Lin_1 1.00 3845454 5 11628 11636

Printed 7:55:28 AM US/Eastern 11/17/2010 Page: 2 of 3

 DILUTOR LINEARITY & ACCURACY REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 050 Dilutor Lin Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Acc PM Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Lin Acc Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11576 DB ID:
Result 847558692
Set Id 11600
Sample Set Name: Dilutor Lin Sample Set Start Date: 11/4/2010 6:12:41 PM

Dilutor L i n e a r i t y R e s u l t s

Calibration Id 11629 Y Intercept 32759.872326

Equation Y = 3.83e+006 X + 3.28e+004 Slope 3834219.771506

Fit Type Linear (1st Order) R^2 0.99968

Caffeine Dilutor Linearity Calibration Plot

4x106
Area

2x106

0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00
Amount
R^2 0.99968

Accuracy Calculation: Dilutor Accuracy = |Y intercept/Slope|

Y intercept = 32760 Slope = 3834220

Dilutor Accuracy = | 32760 / 3834220| = 0.01

Printed 7:55:28 AM US/Eastern 11/17/2010 Page: 3 of 3