LABORATORY MANUAL FOR EXHIBIT

BSMT-1B

PREPARED BY
JUDE BERNARDINO

NOTED BY
GWEN ALEMANIA
RAVEN SARMIENTO

SEPTEMBER 19, 2022

MEDICAL TECHNOLOGY WEEK 2022

Table of Contents
INORGANIC CHEMISTRY 101 .............................................. 3
EXPERIMENT I ...................................................................................................................................... 3
EXPERIMENT II ................................................................................................................................... 4
EXPERIMENT III ................................................................................................................................. 5
EXPERIMENT IV ................................................................................................................................... 6
EXPERIMENT V ...................................................................................................................................... 6
EXPERIMENT VI ................................................................................................................................... 7
EXPERIMENT VII ................................................................................................................................. 8
ORGANIC CHEMISTRY 101 ............................................... 10
EXPERIMENT I .................................................................................................................................... 10
EXPERIMENT II ................................................................................................................................. 11

MEDICAL TECHNOLOGY WEEK 2022

 INORGANIC CHEMISTRY 101
EXPERIMENT I
LAVA LAMP
Lava Lamps are the staple of innocent, simple, and effective
chemistry reaction. One method of preparation is to react vinegar
with sodium bicarbonate (baking soda).

MATERIALS

 2 Glass/Plastic Container
 1 ½ tablespoon of Baking Soda
 Vegetable Oil
 Vinegar Solution
 Liquid Food Coloring
PROCEDURE
Put one and a half (1 ½) tablespoon of Baking Soda on an empty
container. Pour vegetable oil on the container until it is filled.
On a separate container, mix one (1) teaspoon of vinegar and a few
drops of food coloring.
Pour the colorized vinegar solution on the baking soda-vegetable
oil container.
Close the lid of the container.
One can put an LED-lit platform underneath the container to have
a complete lava lamp.

Another way of creating a lava lamp is through reacting aspirin to

oil and water.
MATERIALS

 1 Glass/Plastic Container
 Baby Oil
 Water
 Aspirin Tablet/s

MEDICAL TECHNOLOGY WEEK 2022

  Liquid Food Coloring
PROCEDURE
Fill the container with baby oil until it is half-empty. Pour water
until the glass is full. This should make a visible division
between water and baby oil.
Drop two to three liquid food coloring on the container.
Drop the aspirin tablet and seal the lid.

EXPERIMENT II
SOLAR PRINT
Usually, paint dries when put under the sun—retaining its color.
However, with a solar paint, the inverse could be said. Once a
newly solar painted sheet of paper is dried under the sun, it
vanishes. But, there is a way to retain a specific part of the
solar painted paper.
MATERIALS

 2 Glass/Plastic container
 1 tablespoon of Turmeric Powder
 1 bottle of antiseptic solution/hydrogen peroxide
 Filter paper
 Funnel
 Sheet of paper
 Paintbrush
 Leaf samples
 Plastic cover
 Paper weights
 Hairdryer (optional)
 Contact lens solution (optional)
PROCEDURE
Mix one (1) tablespoon of Turmeric powder and antiseptic in a
glass/plastic container. Pour the mixture into the other
glass/plastic container with a filter paper-covered funnel on it.
Wait until the solution is thoroughly filtered.

MEDICAL TECHNOLOGY WEEK 2022

Remove the funnel from the container. Stir the mixture with the
paintbrush and paint a sheet of paper with it. Dry it with a
hairdryer or in room temperature.
Arrange some of the leaf samples on the paper and fix them in
however one desires. Cover it with the plastic cover and make sure
to put paper weights on them. Leave to dry in the sun for 24 hours.
A yellow pattern from the turmeric’s natural dye called curcumin
remains which can be preserved from further sun exposure using
contact lens solution.

EXPERIMENT III
NYLON 6,10 SYNTHESIS
Nylon is a type of polymer that is widely used in any industry—
from the fishing industry to industrial production. The sole reason
why the tensile strength of nylons is unbelievably high is because
its structure consists of an interconnected chain of monomers—
small units of molecules that when linked together synthesizes
strong bonds. This experiment synthesizes a quick-drying nylon
using two substances.
MATERIALS

 1,6 Hexanediamine
 Sebacoyl Chloride
 100 mL Beaker
 Thin wire with a paperclip end
 Small pincers/Tiyani (ALTERNATIVE for the thin wire)
PROCEDURE
CAUTION: WEAR PROPER PROTECTIVE EQUIPMENT IN THE FIRST HALF OF THE
EXPERIMENT!
Pour about 25Ml OF 1,6 Hexanediamine in the beaker slightly. Pour
in an equal amount of Sebacoyl Chloride atop of the 1,6.
This should form a visible layer between the two liquids. The
polymer will form at the interface between the two liquids. This
can be extracted using a wire with a small hook or with a small
pincer.
Lower the bent paperclip end into the bottom layer and pull up
slowly past the interface. The small pincers can also pinch the

MEDICAL TECHNOLOGY WEEK 2022

bottom layer up past the interface. An observable strand of Nylon
6,10 should be pulled.
The strand pulled can now be washed with water. DO NOT TOUCH THE
NYLON 6,10 STRAND WITH BARE HANDS IF IT HAS NOT BEEN PROPERLY
RINSED. DO NOT MAKE A DIRECT CONTACT WITH THE SKIN.
After rinsing the strand for at least two (2) minutes, it can now
be safely touched and weaved into a durable mesh.

EXPERIMENT IV
SQUISHY BALLS FROM GLUE AND SALT
The wonders of polymers do not stop with nylon synthesis. Using
simply glue and salt, one could make a rubbery ball for
recreational purposes.
MATERIALS

 Glass/Plastic bowl
 25 mL of Silicate glue
 Water
 Table salt
 Stirring rod
PROCEDURE
Pour about 25 mL of Silicate glue into a bowl. Add in an aqueous
solution of water saturated with at least three (3) tablespoon of
table salt.
Stir vigorously for two minutes. The mixture—now moldable—can be
shaped into a ball.
The polymer chains contained within the silicate glue precipitates
once it contacts with the salt solution. This process is called
salting out.
EXPERIMENT V
WATER VAPOR RING CANNON
A simple reaction between water and dry ice should be more than
enough to produce a water vapor cannon.
MATERIALS

 Small balloon
 Scissors

MEDICAL TECHNOLOGY WEEK 2022

  Small paper cup
 Dry Ice
 Water
 Cutter (optional)
PROCEDURE
Cut the balloon horizontally. Cover the opening of the cup with
the body of the balloon. Dispose the neck and lip of the balloon.
Cut a small to medium hole at the base of the cup using the scissors
or the cutter.
CAUTION: WEAR LATEX GLOVES WHEN HANDLING DRY ICE!
Put one or two pieces of dry ice inside the cup. Add a bit of
water.
A dense white fog should be generated. Strike the balloon until
white rings appear. The sublimation—a process when a solid turn to
gas—of dry ice inside the cup produces the thick white fog.
As the dry ice come in contact with the water, bubbling occurs
that captures small water droplets until a thick white vapor
appears. And every time the balloon is struck, the vapor leaves
the cup. The small to medium hole shapes the vapor into a circle.
As it leaves out of the cup, the edges travel slower than the
center hence producing a hole.

EXPERIMENT VI
GLUCOSE REDUCTION IN ALKALINE MEDIUM
A step-by-step guide on how to turn blue liquid into a transparent
liquid with a few shakes.
MATERIALS

 Bottle with stopper

 10% Sodium Hydroxide solution
 Methylene Blue solution
 Glucose
PROCEDURE
CAUTION: WEAR PROPER PROTECTIVE EQUIPMENT IN HANDLING NaOH!

MEDICAL TECHNOLOGY WEEK 2022

Place the glucose in the bottle, then add the sodium hydroxide
solution. DO NOT MAKE A DIRECT CONTACT WITH THE SKIN. And then add
the methylene blue solution. Mix together, shaking the bottle. The
solution turns colorless. Shake the bottle again and the solution
turns blue.
Methylene blue is an organic dye and indicator which easily reduces
to a colorless state. As a reducer, glucose in an alkaline medium
can be used. It reduces methylene blue to a colorless form. If you
shake the bottle, the oxygen contained in the air oxidizes the
colorless form back to a colored form.
Then a reverse transformation to a colorless form takes place,
until all of the glucose has oxidized to gluconic acid.

EXPERIMENT VII
CHARCOAL & SAND FILTRATION
In a do or die scenario, every resources are important. In this
experiment, pond water will be filtrated using only sand, charcoal,
a small cotton ball, and a plastic bottle.
MATERIALS

 100 g of charcoal
 300 g of sand
 Plastic bottle with a lid
 Stationery knife
 Cotton wool
 Betadine(Povidone-Iodine) (optional)
PROCEDURE
Grind off the charcoal to powdered bits.
Cut the bottom off the plastic bottle. Unscrew the lid of the
bottle, make a hole in it with a knife and place cotton wool in
the lid.
Close the bottle and turn it upside down. Place three (3)
tablespoon of sand and charcoal in the bottle. Repeat this until
you have a sand-charcoal-sand-charcoal-sand-charcoal layers. Be
advised that the more layers, the better the purification will be.
Since dirty water contains many organic and mineral impurities,
and also various insoluble particles of dust and earth, the sand

MEDICAL TECHNOLOGY WEEK 2022

purifies the water of insoluble particles. Charcoal has good ad-
sorption and so adsorbs organic and mineral impurities.
It must be noted, however, that this filter has a considerable
drawback: it does not purify the water from bacteria and heavy
metals. So after filtration the water should be boiled well—
preferably with a few drops of betadine (povidone-iodine.)

MEDICAL TECHNOLOGY WEEK 2022

 ORGANIC CHEMISTRY 101
EXPERIMENT I
SYNTHETIC CRYSTALLIZATION
Hot water + Copper Sulfate = Blue Gems
MATERIALS

 70g of Copper Sulfate

 100 mL of hot water
 Beaker
 Stirring rod
 Funnel
 Filter paper
 Aluminum foil
PROCEDURE
CAUTION: WEAR PROPER PROTECTIVE EQUIPMENT IN THIS EXPERIMENT!
Sprinkle copper(II) sulfate pentahydrate into a beaker and pour
hot water over it. Stir thoroughly for 10-15 minutes until there
is a saturated solution.
Filter the solution from solid impurities and dust through the
funnel and filter paper. Cover the solution with foil and leave in
a dark place for 24 hours.
Then pour the solution into another beaker and take out the crys-
tals that have formed.
In a saturated solution, the substance is at maximum concentration
and does not dissolve further at the given temperature. At room
temperature (25°С), the solubility of copper(II) sulfate pentahy-
drate in water is around 35 g/100 g of water.
When heated to 90 °С, solubility increase to 100 g/100 g of water.
So when it cools the solution becomes saturated. It means more of
the substance is dissolved in it at the given temperature. As a
result, the “excess substance”–copper(II) sulfate pentahydrate in
our case – precipitates in the form of crystals, and the solution
once more becomes saturated.
If you place a crystal in this solution, it will not dissolve, but
become covered with ions of the dissolved salt.

MEDICAL TECHNOLOGY WEEK 2022

 EXPERIMENT II
PAPER CHROMATOGRAPHY
Chromatography is a method of separating and analyzing substances
based on the different mobility of substances between two phases
– a stationary phase (in this case, the filter paper) and a mobile
phase (the citric acid solution). However, it is still viable to
use in art and crafts.

MATERIALS

 Filter papers
 Marking pens of various colors
 Alcohol
 Plastic bowl
PROCEDURE
Use the marking pen to draw a spot on a sheet of filter paper.
Pour isopropyl alcohol into a plastic bowl. Rest the filter paper
on the rim on the bowl so that the ink touches the alcohol. The
markings will turn into multicolored rings.
Due to the capillary effect, the alcohol solution evenly wets the
paper and carries the marker dye with it, which divides into sev-
eral colors as it moves farther from the center. The dyes compris-
ing the marker dye in the pen differ from each other in their
chemical nature, which is why they move through the paper at dif-
ferent speeds.

MEDICAL TECHNOLOGY WEEK 2022