Principles of Genetic 2.

Competent host
engineering
rDNA (recombinant
DNA)
Gene cloning
(increasing gene
copies using vectors)
Gene transfer
(transfer of DNA
segment from one
organism to another)
Obtaining foregin gene
product by bioreactors.
Host is made competent by:
Biolistics (Cell are
bombarded with high velocity
micro particles of gold or
tungsten coated with DNA)
Micro injections (rDNA is
directly injected into the
1.Cloning vector nucleus of an animal cell)
Features Disarmed pathogen vector
Ori site (Vectors like Agrobacterium
Selectable when infects the cell transfer
Marker the recombinant DNA into the
Fewer cloning host)
sites

Transfer of rDNA into the Processes 3. Restriction

host cell. of rDNA enzymes
technology
Tools of
Cutting of DNA at specific Biotechnology rDNA
locations by restriction Technology
enzymes. : Principles
and Processes

It is the integration of natural

science and organisms, cells,
parts thereof, and molecular
analogues for product and
services.