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Rtanlaii Avilaetal2010RhinellaparaguayensisZootaxa
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Christine Strüssmann
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Abstract
We describe adult morphology, advertisement call and some natural history traits of a new species of toad from the
Pantanal, western Brazil. Rhinella paraguayensis sp. nov. belongs to the Rhinella margaritifera group, and is
characterized by medium size, snout rounded in dorsal view, with a vertical apical ridge, supraorbital crests weakly
developed, parietal crest not well developed, postorbital crest prominent, presence of a dorsolateral line of tubercles,
tympanum evident, bony protrusions at angle of jaws, absence of vertebral apophyses and of projections on upper
eyelids, and parotoid glands small. The new species is distinguished from other species of the group by geographic
distribution and by the use of floating mats of vegetation as reproductive site.
Introduction
Species of the Rhinella margaritifera group are medium-sized toads, inhabitants of forest leaf litter in Central
and South America (Frost 2009). This group remains problematic taxonomically in spite of recent efforts for
elucidating the many fuzzy species boundaries. The difficulty lies principally in the cryptic morphological
diversity and a lack of diagnostic characteristics for distinguishing the species (Caramaschi & Niemeyer
2003; Caramaschi & Pombal 2006; Fouquet et al. 2007b; Lima et al. 2007). According to Fouquet et al.
(2007b) 14 species are currently recognized: R. acutirostris (Spix), R. alata (Thominot), R. castaneotica
(Caldwell), R. dapsilis (Myers & Carvalho), R. hoogmoedi Caramaschi & Pombal, R. lescurei Fouquet,
Gaucher, Blanc & Velez-Rodriguez, R. magnussoni Lima, Menin & Araújo, R. margaritifera (Laurenti), R.
martyi Fouquet, Gaucher, Blanc & Velez-Rodriguez, R. proboscidea (Spix), R. roqueana (Melin),R.
sclerocephala (Mijares-Urrutia & Arends), R. scitula (Caramaschi & Niemeyer), and Rhinella stanlaii
(Lötters & Köhler). Out of the 14 species, 12 occur in Amazonia, while R. hoogmoedi occurs in the Atlantic
forest and R. scitula in gallery forests in the Cerrado (Caramaschi & Niemeyer 2003; Caramaschi & Pombal
2006; Lima et al. 2007). Herein, we describe adults, advertisement calls, and provide information on natural
history of a new species belonging to this group and found in the Pantanal, western Brazil.
We examined 20 specimens housed in the Coleção Zoológica de Vertebrados of the Universidade Federal de
Mato Grosso (UFMT, Cuiabá, Mato Grosso State, Brazil). Specimens in the type-series were collected in the
Results
Holotype. UFMT 7430, adult male (Figure 1), collected on 15 May 2001 by Christine Strüssmann at “Parque
Nacional do Pantanal” (17º51'12”S; 57º24'39”W), Poconé municipality, Mato Grosso state, Brazil.
FIGURE 1. Holotype of Rhinella paraguayensis sp. nov. (UFMT 7430): A. dorsal view; B. ventral view; C. profile of
head; D. ventral view of right hand; E.ventral view of right foot.
Paratypes. UFMT 4268, 4270 (adult males), UFMT 4272, 4274, 4276 (adult females), 11–12 May 2001,
by Tami Mott, Raul Vieira, Christine Strüssmann; UFMT 7145 (adult male) and 7150 (adult female), 11–15
September 2001, by Christine Strüssmann and Vinicius T. Carvalho; UFMT 4269 (adult male) and 4271
(adult female), 27 March 2001, by Rogério Rossi, Ana P. Carmignotto and Mário M. Rollo Junior; UFMT
7056 and 7062 (juveniles), 13–22 September 2001, by Vinicius T. Carvalho, all from the same locality as
holotype. UFMT 046 (juvenile), May 1995, by C. Strüssmann and UFMT 171 and 172 (juveniles), 20 August
1982, by J. C. Dalponte from “Base de Pesquisa da Fauna do Pantanal, IBAMA”, km 111 of “Rodovia
Transpantaneira" (17°10’S, 57°00”W), Poconé, Mato Grosso state, Brazil. UFMT 2112 (adult male), 02
March 2002, by Christine Strüssmann in Cáceres municipality (15°47’31”S, 57°35’28”), Mato Grosso state,
TABLE 1. Measurements (mm) of holotype and of specimens in the type series of Rhinella paraguayensis sp. nov.
Means are ± 1 standard deviation, ranges are in parentheses. Abbreviations are defined in the material and methods
section.
Character Holotype Male (n = 6) Female (n = 5)
SVL 50.4 47.5 ± 3.5 (42.3–52.6) 52.4 ± 1.0 (51–53.3)
HL 17.2 16.6 ± 1.3 (14–18.1) 17.8 ± 0.9 (16.9–18.9)
HW 17.1 18.0 ± 1.4 (16.6–20.1) 17.8 ± 2.0 (15.5–20.2)
IND 4.0 3.1 ± 0.5 (3.2–3.5) 3.3 ± 0.2 (2.9–3.5)
END 4.1 4.8 ± 0.6 (3.8–5.6) 4.7 ± 1.0 (3.5–6)
ED 4.0 4.6 ± 0.6 (4–5.4) 4.7 ± 0.5 (4–5.3)
UEW 3.7 3.4 ± 0.2 (3–3.7) 3.2 ± 0.6 (2.4–3.9)
IOD 16 14.6 ± 1.1 (13–16.1) 15.5 ± 0.5 (15.2–16)
POCL 5.2 4.7 ± 0.5 (4.2–5.2) 5.6 ± 0.3 (5.3–6)
HTD 2.8 2.4 ± 0.6 (1.6–3.1) 2.7 ± 0.6 (1.9–3.3)
VTD 3.7 3.1 ± 0.4 (2.5–3.7) 3.2 ± 0.4 (2.8–3.7)
PGL 9.2 7.8 ± 1.3 (6–9.2) 7.9 ± 1.3 (6.8–9.9)
HAL 14.2 13.3 ± 1.0 (12–14.3) 14.0 ± 0.5 (13.5–14.8)
THL 13 11.1 ± 1.6 (8.8–13) 13.1 ± 2.0 (11–15.5)
TL 18 16.3 ± 1.6 (14.7–19) 17.9 ± 1.8 (16.5–20.3)
FL 22.3 24.9 ± 2.0 (22.3–28) 26.4 ± 2.9 (21.6–29.2)
Hindlimbs robust, thigh length smaller than tibia length, thigh+tibia length approximately 62% of the
SVL; tarsus+foot length longer than tibia and thigh length; a line of tubercles on external and internal borders
of tarsus. Inner metatarsal tubercle oval, two times larger than the outer metatarsal tubercle; subarticular and
supernumerary tubercles present, small, round. Length of toes 1<5<2<3<4, webbing formula: I 1-2 II 1-3 III
1-2 ½ IV 2 ½-1V.
Colour in preservative: dorsum light brown, dark brown on legs and head; a yellowish vertebral line from
the snout to cloaca, surrounded by three black triangular spots at midbody and a pair of triangular black spots
at the level of the parietal crest. Venter uniform brown; throat, hand, foot and legs dark brown; an irregular
dark brown band in the forearm. Color in life (Figure 2A) similar to that in preservative. Iris gold, with black
reticulations.
Variation within type series. The type series include 20 specimens (6 adult males, 5 adult females and 9
juveniles). Dorsal coloration varies a lot among individuals in the type series (Figure 3), with the fine
vertebral line present in 75% of the individuals and only one individual (holotype) have a wide vertebral line.
Few specimens don’t have the triangular spots in the midbody. The ventral coloration is also variable, from
the uniform brown to spotted bellies.
Advertisement call. The call of the new species is composed by 18.4 ± 8.7 call series (9–30 calls, n=5),
with duration of call series of 16.5 ± 7.2s (n=5), emitted at regular intervals of 10.4 ± 0.8s (n= 5). The call of
R. paraguayensis sp. nov. is composed by mean 6.5 ± 0.65 notes (5–8 notes, n=77) , with a mean duration of
0.3s ± 0.04s (0.2–0.42, n=82), followed by intercall intervals of 0.5s ± 0.015s (0.22–0.98, n=82). The average
rate of call per minute was 39.3 ± 6.4. The mean duration of note was 0.03s ± 0.01s (0.01–0.07, n=163),
followed by inter-notes intervals of 0.02s ± 0.01s (0.01–0.04, n=163). The average rate of notes per minute
was 504.8 ± 113 (262.4–792.4). Frequency ranges from 618.9–1771.8 Hz (n=125); mean dominant frequency
was 1438.7 ± 70.5 Hz (1113.7–1568.5, n=125), displayed between the fourth and fifth note (Figure 4).
Natural history. In the Upper Paraguay River Basin, individuals of Rhinella paraguayensis sp. nov.
inhabit the leaf litter of gallery forests, and of forests established over paleo-levees throughout the Pantanal
wetlands. Riparian systems are used as reproductive sites by individuals in this new species (Figure 5), which
apparently presents a unimodal pattern of reproduction, as shown below.
During the peak of the floods, in April-May, which are coincident with the beginning of the dry season,
the Paraguay River discharges increased (Barros et al. 2004), facilitating the movements of dense stands of
macrophytes (particularly the water hyacinth Eichhornia azurea; Figure 5). These macrophyte stands offer
both vocalization perches for calling males and lentic habitats for the deposition of egg clutches, as well as
protection for the tadpoles. Additionally, the displacement of macrophyte stands downriver may facilitate the
transport and dispersal of tadpoles, juveniles and also adults of R. paraguayensis sp. nov., as already reported
for several amphibian species using macrophytes as rafts in others river systems (Hoogmoed, 1993; Schiesari
et al. 2003).
Observations of calling males occurred on seven occasions. In 10 June 1989 a male calling from nude
ground at “Rodovia Transpantaneira”, Poconé, Mato Grosso. In April and May 2001, males of the new
species were observed calling on floating stems and leaves of E. azurea at “Parque Nacional do Pantanal”,
Poconé municipality, Mato Grosso. From 4 March 2002 males were observed calling (a male was recorded
and used in the call description) in a flooded swampy areas in the left margin of the Sepotuba River, Cáceres
municipality, Mato Grosso. Between 14–17 May 2002 several males of R. paraguayensis sp. nov. were
observed calling on nude ground in the Paraguay River banks, approximately 0.5 m from the river margin, in
FIGURE 5. A. General view of a riparian system along the margins of the Paraguay River near Acurizal Ranch
(17°49’51” S; 57°33’06” W), in the western border of the Brazilian Pantanal, Poconé municipality, Mato Grosso state.
This kind of riparian system, formed by gallery forests and dense stands of macrophytes anchored along the margins of
river, is used by individuals of Rhinella paraguayensis sp. nov. as a reproductive site, in the peak of the dry season.
Therefore, calling activities of Rhinella paraguayensis sp. nov. in the Pantanal were recorded from March
to October and seems to be correlated with the Paraguay River flooding cycle .
Rhinella paraguayensis sp. nov. is usually abundant in the localities where the species was already
recorded. In a field study in the Parque Nacional do Pantanal, it was the third most abundant among the 13
anuran species recorded by pitfall trapping (http://www.ibama.gov.br/siucweb/unidades/parna/
planos_de_manejo/78/index.htm).
Distribution. The new species occurs along the western drainages of the Paraguay River, in the Brazilian
states of Mato Grosso and Mato Grosso do Sul (Figure 7).
Etymology. The specific epithet is an adjective, derived from the type locality of the new species: the
Paraguay River basin.
FIGURE 7. Geographical distribution map of Rhinella paraguayensis sp. nov (triangles) and R. scitula (circles). Star =
type locality of Rhinella paraguayensis sp. nov and square = type locality of R. scitula.
Discussion
The Rhinella margaritifera complex remains as one of the major problems in South American anuran
taxonomy (see Fouquet et al. 2007a). There are several undescribed species, and the cryptic morphology
amongst representatives of the group turns difficult the systematic arrangement even of the species already
described (Hoogmoed 1989, 1990; Lima et al. 2007). Nevertheless, recent studies have demonstrated that
genetic and acoustic differences can be useful tools for differentiating species in this group (Lima et al. 2007;
Fouquet et al. 2007b).
The dominant frequency in calls of R. paraguayensis sp. nov. is similar to what was recorded in Rhinella
sp. clade E (1407 Hz), and higher than in R. martyi (1169 Hz), R. lescurei (1161 Hz), and R. margaritifera
(1265 Hz) (Fouquet et al. 2007b) and in a representative of the Rhinella margaritifera complex in Bolivia
(1332 Hz; De la Riva et al. 1996). Dominant frequency is considerably lower in R. paraguayensis sp. nov.
than in R. castaneotica (1650 Hz; Köhler & Lötters 1999). Frequency range is lower than in R. magnussoni
(1600–2820 Hz; Lima et al. 2007), R. castaneotica (900–2600 Hz; Köhler & Lötters 1999), and R.
proboscidea (1620–3200 Hz; Zimmerman & Bogart 1988) and similar to the Bolivian populations assigned to
the Rhinella margaritifera complex (500–1500; De la Riva et al. 1996). Note duration was lower than R.
magnussoni (0.06 ± 0.01 s); Lima et al. 2007) and R. proboscidea (0.12 s; Zimmerman & Bogart 1988).
Moreover, there are clear ecological differences between some of the species in the Rhinella margaritifera
species group, including geographic distribution, reproductive habitat, and vocalization microhabitat (Lima et
al. 2007). Species in the R. margaritifera complex are distributed mainly in the Amazonia Biome, with a few
species occurring in other forested (e.g. Atlantic Forest – R. hoogmoedi Caramaschi & Pombal 2006) or
opened ecosystems (e.g. Cerrado (Brazilian savanna) – R. scitula Caramaschi & Niemeyer 2003; Pantanal –
R. paraguayensis sp. nov., present study; Figure 7).
Acknowledgements
We thank PROBIO, Ecotrópica, IBAMA, for partial financial support to field expeditions; José Augusto
Ferraz de Lima, for the arrangements to work at the Pantanal National Park; Pierre Girard for the help to
obtain discharges data for Amolar and Ricardo A. Kawashita Ribeiro for preparing the distribution map.
Masao Uetanabaro for providing photographs of live specimen of R. scitula and Franco L. Souza for gently
provided voucher specimens of R. scitula.
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