IGF-1, bovine/porcine, LR3, recombinant

Catalog Number: MK0301

DESCRIPTION
The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types,
including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of
tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and
relaxin. The IGFs are similar to insulin by structure and function but have a much higher growth-promoting activity than
insulin. IGF-I LR3 is a recombinant analog of human IGF-I comprised of the complete IGF-I sequence, with an Arginine
substitution for the third position Glutamic acid, and a 13 amino acid length N terminus peptide extension. Specifically
engineered for higher biological potency in vitro, IGF-I LR3 has an increased half-life and a binding aversion to native
proteins within the body that make it ideal for both research and large-scale culturing.

SOURCE
Recombinant MESOkine® IGF-1 LR3, bovine/porcine is produced in the endosperm tissue of barley seed (Hordeum
vulgare).

FORMULATION
Tris pH 7.4, sterile filtered through 0.2 um filter.

PURITY
MESOkine® recombinant IGF-1 LR3 is purified from barley seeds resulting in an extract containing purified recombinant
IGF-1 LR3 protein along with co-purified barley seed proteins. The amount of the recombinant IGF-1 LR3 protein is
typically in the range of 20-30% of total purified protein content. The exact content of the growth factor is stated on the
vials. The co-purified barley seed proteins may serve as stabilizing proteins that can prolong the lifetime of the
recombinant protein during proper storage and may enhance the bioactivity of growth factors in standardized
bioassays.

RECONSTITUTION & STABILTY

It is recommended to reconstitute the lyophilized protein in sterile buffered saline or a solution of your choice, preferably
to a concentration of no more than 10-100 µg/ml. If needed the reconstituted material may be centrifuged at 3500G for 5
min or filtrated using 0.45 micron filter. The lyophilized protein, though stable at room temperature for few weeks, is best
stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated
freeze-thaw cycles.

BIOLOGICAL ACTIVITY
Each batch of MESOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial
source. Bioactivity is assayed by measuring the dose dependent effect of recombinant human MESOkine® IGF-I LR3 on
proliferation of FDC-P1 cells, murine progenitor cells of granulocytes, monocytes and macrophages. The ED50 for this
effect of using recombinant human MESOkine® IGF-I LR3 is typically below 0,1 ng/ml, corresponding to specific activity of
> 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

MOLECULAR WEIGHT
MESOkine® IGF-1 LR3, recombinant, human, is a non-glycosylated polypeptide chain containing 83 amino acid residues
and a 14 a.a. Histidine-based tag for a total length of 97 a.a. with a predicted total molecular mass of 10.76 kDa. The
recombinant protein migrates with an apparent molecular mass of 12.5 kDa in SDS-PAGE.

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