LABORATORY INTRODUCTION

HISTOLOGY OF URINARY SYSTEM

 OBJECTIVES
At the end of this laboratory you should be able to:
• Identify the kidney, ureter, and urinary bladder
microscopically.
• Distinguish between the cortex and medulla of the kidney.
• Identify the segments of the nephron, ie, the proximal
tubule, distal tubule, and Bowman's capsule with the
glomerulus and the loop of Henle.
• Identify medullary rays, distinguishing them from other
regions of the kidney cortex
• Identify the macula densa and understand its relationship
to the glomerulus.
• Identify the urinary and vascular poles of Bowman's
capsule.
 Kidney
• Observe the cortex , medulla , and delicate connective tissue capsule .
• Renal corpuscles (glomerulus + Bowman's capsule) as well as
the proximal and distal convoluted tubules are found in the cortex. One
can distinguish proximal convoluted tubules from distal convoluted
tubules by these characteristics:
– Staining - proximal tubules are more eosinophilic than the distal.
– Nuclei - proximal tubule cells have more heterochromatic nuclei than those of
distal tubule cells; and there are more nuclei per unit area in the distal tubule
than there are in the proximal tubule.
– Lumen – distal tubule larger than proximal tubule
– Cytoplasm - the distal tubule cells appear with less cytoplasm.
– Microvillus "brush" border - in the proximal convoluted tubules the “brush”
border has sloughed and can be seen in the lumen; thus, the lumen appears
partially filled.
• Observe the medullary rays and identify collecting tubules within the rays.
• Identify the vascular and urinary poles of the renal corpuscles. Also
observe the parietal layer of Bowman's capsule , and the urinary space
Ginjal korteks dan medulla-VIDEO
Slide U2 (Kapsul ginjal)
Korteks Ginjal (Video)
Korteks ginjal-renal corpuscle
40x (medulla ginjal)
100x (medulla ginjal)
400x (medulla ginjal-collecting duct)
 Ureter

• Observe the transitional epithelium.

• Examine the layers of smooth muscle. Inner
longitudinal and outer circular layers are
distinguishable in this slide.
40x (ureter)
100x (ureter)
400x (ureter-epitel)
Slide U3 400x (otot polos dan jaringan
ikat)
 Urinary Bladder
• Note the mucosa, muscularis, and
the adventisia. Observe the inner longitudinal,
middle circular and outer longitudinal bands
of smooth muscle in the muscularis.
• Examine the transitional epithelium with its
characteristic dome shaped surface cells.
(VIDEO) Urinary Bladder
Slide U4 40x (Urinary Bladder)
Slide U4 100x (Urinary Bladder)
Slide U4 400x (Urinary Bladder-epitel
transitional)
Slide U4 400x (Urinary Bladder-
jaringan ikat subepitel)
Slide U4 400x (Urinary Bladder-otot
polos)