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AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA

GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)


Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Neoplasia

Subject Material: Microscopic picture

For candidate:

A 55 year old lady developed an ulcerated lesion on her face. Biopsy of the lesion shows the
following picture.

Task
Q.1. Identify the given picture of slide? 1
Q.2Which virus is associated with this tumor? 1
Q.3.Enlist salient histological features. 2

For Examiner
Q.1. Squamous cell Carcinoma 1
Q.2. Human papilloma virus 1
Q.3. Nuclear atypia and hyperchromasia, loss of polarity, keratinization, intercellular bridges,
necrosis . 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/cellular adaptations

Subject Material: photograph

For candidate
Examine the given photograph and answer the following questions
Q.1. Name the type of cellular adaptation and define it. 02

Q.2. What is the cause of this cellular adaptation. 01

Q.3. Name two other types of cellular adaptations. 01

For Examiner

Q.1.Hypertrophy. Increase in size of organ due to increase in cell size. 02

Q2. Increased workload causes hypertrophy of muscles. 01

Q.3. Atrophy, hyperplasia. 01


AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Acute and Chronic inflammation

Subject Material: Clinical scenario and Microscopic picture

For candidate:

A 25-year old girl presented with fever and productive cough . On examination, her cervical
lymph nodes were found to be enlarged. Biopsy of lymph node shows features given in the
diagram.

Task
Q. 1. What type of inflammation occur sin this case. 1
Q. 2. What is the type of necrosis? 1
Q. 3. Name the disease / condition. 1
Q. 4. Name the etiological agent which incites such reaction. 1

For Examiner:
Q. 1. Chronic inflammation. 1
Q. 2. Caseous necrosis 1
Q. 3. Chronic granulomatous (or tuberculous) inflammation / Tuberculosis. 1
Q. 4. Mycobacterium tuberculosis 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Acute and Chronic inflammation

Subject Material: Clinical scenario and specimen

For candidate:

Microscopic slide of biopsy of an appendix from a patient with right iliac fossa pain.
Carefully examine the focused slide and answer the following questions:

Q1. Give your diagnosis 01


Q2. Name the predominant cells seen in this condition 01
Q.3. Give names of two chemical mediators causing pain. 01
Q.4. Name any two chemical mediators causing leukocytes chemotaxis. 01

For Examiner

Q..1.Acute Suppurative Appendicitis 1


Q.2. Neutrophils. 1
Q.3. Prostaglandins &Bradykinin. 1
Q.4. C3a, C5a, IL-8, Leukotriene B4 TNF, IL-1. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Acute and Chronic inflammation

Subject Material: Clinical scenario and specimen

For candidate:
Examine the scanned photograph of liver and answer the following questions
Q.1. Identify the condition. 01
Q.2.What is the underlying cause of such appearance of liver in this patient? 02
Q.3. What are heart failure cells? . 01

For Examiner

Q.1. Nutmeg liver (chronic hepatic congestion) 1


Q.2 Chronic passive hepatic congestion due to congestive cardiac failure, the centrilobular
regions are grossly red-brown and slightly depressed (because of cell death) and are
accentuated against the surrounding zones of uncongested tan liver. 2
Q.3.In chronic pulmonary congestion the septa are thickened and fibrotic, and the alveoli
often contain numerous hemosiderin-laden macrophages called heart failure cells. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Neoplasia

Subject Material: Clinical scenario and photograph

For candidate:

The photomicrograph shows a tumor excised from breast of a 28 year old girl. The tumor was
1.5 cm in size, mobile and circumscribed with smooth margins. The cut section is shown

Task

Q. 1. What is the most likely diagnosis. 1


Q. 2. Name any other benign condition of breast? 1
Q. 3. Mention two important histological features of this tumor. 2

For Examiner:

Q.1.Fibroadenoma 1

Q.2. fibrocystic changes 1

Q.3.Encapsulation, slit-like spaces and fibrous response 2


AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/ Cellular adaptations

Subject Material: photograph

For candidate:

The gum of this person is showing hyperplasia. Examine the given photograph and answer
the following questions

For candidate

Q.1.Define hyperplasia? 1
Q.2. what is the difference between hyperplasia and hypertrophy. 1
Q.3. Name two examples of pathological hyperplasia. 2

For Examiner
Q.1. Increase in size of organ due to increase in cell number. 1
Q.2. Hypertrophy is increase in size of organ due to increase in cell size while hyperplasia is
Increase in size of organ due to increase in cell number . 1
Q.3. Endometrial hyperplasia, benign prostatic hyperplasia. 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General pathology/ Neoplasia

Subject Material: clinical scenario and specimen

For candidate

Task

The above picture shows the cut surface of Liver of a patient with gastric adenocarcinoma.

Q.1.Identify the disease process shown in this picture. 1


Q.2. What other sites can be commonly involved? 1
Q.3. Give two features of malignant tumors. 2

For Examiner

Q.1. Metastasis. 1

Q.2.Brain, Bone, Lungs. (Any two) 1

Q.3. High mitotic rate, Poor differentiation, metastasis 2


AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For organizer

Topic Specification: General Pathology/Wound Healing

Subject Material: Photograph

For candidate

A 40 year old woman developed a deformity of the wrist joint after a burn incident.

Q. 1. Identify the condition. 01

Q.2. What is the cause of this deformity. 01

Q.3. Name THREE other complications of wound healing. 02

For Examiner:

Q.1. Wound contracture 1


Q.2. Excessive contraction of wound. 1
Q.3.i) hypertrophic scar 2
i) wound dehiscence.
ii) keloids
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/ Hemodynamic Disorders

Subject Material: specimen

For candidate

Task

1. Identify the condition? (01)


2. Give other two types of infarcts. (01)
3. What is the cause of this condition. ( 01)
4. Name the dominant histologic characteristic of above condition ? (01)

For Examiner:

Q1. White infarct spleen 1

Q.2. Red infarct, septic infarct 1

Q.3. Occlusion of arterial supply 1

Q.4. ischemic coagulative necrosis 1


AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/ Hemodynamic Disorders

Subject Material: specimen

For candidate:

The picture shows cut section of the colon . Examine the given photograph and answer the
following questions.

Task

Q. 1. Identify the type of hemorrhage and define it. 2

Q. 2. Name other two types of hemorrhages 2

For Examiner

Q. 1. Petechiae. Minute 1- to 2-mm hemorrhages into skin, mucous membranes, or serosal


surfaces are called petechiae. 2

Q. 2. Purpura, ecchymoses. hematoma 2


AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Neoplasia

Subject Material: Specimen

For candidate

Task
A 30 years old female presented with primary infertility and menorrhagia. She underwent
surgery and a mass was removed from the uterus. Cut section is shown in the photograph

Q.1. Examine the specimen and give your diagnosis? (01)


Q.2. Write the microscopic features? (02)
Q.3. Can it be malignant? (01)

For Examiner:

Q.1. Leiomyoma 1

Q.2. well-differentiated tumor contains interlacing bundles of neoplastic smooth muscle cells
that are virtually identical in appearance to normal smooth muscle cells of myometrium. 2

Q.3. No 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Neoplasia

Subject Material: Microscopic picture

For candidate

Task

A 50 year old man has a 2x 2 soft mobile cm lump on his shoulder . The lump was removed
and the cut section is shown in the figure

Q.1.Examine the focused microscopic slide and identify. 1


Q.2. Is it a benign or malignant lesion. 1
Q.3. Name one other painless soft tissue swelling. 1
Q.4. Give two differences between benign and malignant tumors. 1

For Examiner
Q.1. Lipoma 1
Q.2. Benign 1
Q.3. Fibroma 1
Q.4. Malignant tumors show lack of differentiation, high mitotic rate and can metastasize
while benign do not. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/ cellular adaptations

Subject Material: Specimen

For candidate:

Gross specimen of the brain in an 82-year-old male with atherosclerotic cerebrovascular


disease, is shown in the figure . Examine the given photograph and answer the following
questions.

Task
Q. 1. Name the condition. 1
Q.2. Is it a physiological or pathological condition. 1
Q.3. Give any four causes of such adaptive process. 2

For Examiner
Q. 1. Cerebral atrophy. 1
Q.2. Pathological 1
Q. 3. Decreased workload (atrophy of disuse), Loss of innervation (denervation atrophy),
Diminished blood supply, inadequate nutrition, pressure &Loss of endocrine stimulation. 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/ Intracellular accumulations

Subject Material: Microscopic picture

For candidate

A 60 year old known diabetic man died and autopsy of the liver shows the following
pucture. Examine the microscopic picture and answer the given questions

Task

Q.1. identify the condition. 1


Q.2. Give two causes of this condition. 1
Q.3. What is the special stain used to diagnose this condition 1
Q.4. Give the microscopic findings of this condition. 1

For Examiner

Q.1. Fatty change in liver 1


Q.2. Alcohol, viral hepatitis, Obesity, Diabetes 1
Q.3. Congo red stain 1
Q.4. Accumulation of fat as small vacuoles in the cytoplasm around the nucleus , creating
cleared spaces that displace the nucleus to the periphery of the cell . 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer
Topic Specification: General Pathology/Acute and Chronic inflammation

Subject Material: Clinical scenario and specimen

For candidate

A 55-year old obese female presented with pain right hypochondrium. After clinical,
radiological and laboratory evaluation,. her cholecystectomy was done. The microscopic
appearance of her gall bladder after cholecystectomy is shown in the picture.

Task
Q. 1. Name the condition. 1
Q. 2. Give major histological features. 2
Q. 3. Give two causes of chronic inflammation. 1

For Examiner
Q 1. Chronic cholecystitis 1
Q.2. Mucosal hyperplasia, lymphocytic infiltrate, thickened muscularis properia. 2
Q.3. Persistent infections , immune mediated inflammatory diseases. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/ Cellular adaptations

Subject Material: Microscopic picture

For candidate

Photomicrograph of a prostatic tissue of a 55year old man is shown below.

Task
Q. 1. Name the condition. 1
Q. 2. Give one example of physiological hyperplasia. 1
Q. 3. Briefly mention salient histological features. 2

For Examiner
Q. 1. (Benign or Nodular) Prostatic Hyperplasia 1
Q. 2. Hormonal hyperplasia in female breast during pregnancy. 1
Q. 3. Glandular proliferation, cystic dilatation, luminal secretion, fibromuscular hypertrophied
stroma and nodularity. 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer:

Topic Specification: General Pathology/Neoplasia

Subject Material: Clinical scenario and photograph

For candidate:

A 65-year man presented with a an ulcerated lesion on his right cheek for 10-months . The
biopsy of the lesion shows the following picture

Task
Q. 1. Name the tumor. 1
Q. 2. What other name is commonly given to the tumor? 1
Q. 3. Enlist two salient histological features of this tumor. 1
Q.4.What does arrow indicate? 1

For Examiner
Q. 1. Basal cell carcinoma 1
Q. 2. Rodent ulcer 1
Q.3. Groups of basaloid cells, peripheral palisading arrangement of cells. 2
Q.4. Palisading arrangement of cells. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: General Pathology/Hemodynamic disorders

Subject Material: Specimen

For candidate

Examine the scanned photograph of heart chamber showing a thrombus. and answer the
following questions
Task
Q.1. What is this type of thrombus called as (01)
Q.2. What is the difference between mural thrombus and vegetation. (01)
Q.3.Give TWO possible outcome of the condition? (02)

For Examiner:

Q.1. Mural thrombi 1


Q.2. Mural thrombi are present in heart chambers while vegetations are thrombi present on
heart valves. 1
Q.3. Propagation, embolization, dissolution ,organization 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer
Topic Specification: General Pathology/ Cellular accumulations
Subject Material: photograph
For candidate
The microscopic examination of the section from meningioma of brain reveals basophilic
structures as shown in the picture.

Task: Carefully examine the slide/photomicrograph and answer the following questions.

Q.1. What is the underlying pathology? 01


Q.2. What does arrow indicate? 01
Q.3. Enlist four causes of metastatic calcification 02

For Examiner

Q.1. Dystrophic calcification 1


Q.2. Psammoma bodies. 1
Q.3. The hypercalcemia is the underlying pathogenetic mechanism for metastatic
calcification. The various causes are: Primary hyperparathyroidism, PTH related Peptide
secretion from tumors, Bone metastasis by tumors, multiple myloma, Vitamin D intoxication,
Prolonged immobilization, milk alkali syndrome, Sarcoidosis, Chronic renal failure. 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer
Topic Specification: General Pathology/ Wound healing
Subject Material: Clinical scenario
For candidate
A pet dog bites a young boy on his right leg. On examination wound on the calf muscle is
deep with lacerated edges.

Task:
Q.1. What is your opinion this wound must heal by first or second intention. 1
Q.2. Enumerate two complications of cutaneous wound healing. 1
Q.3 Enumerate four local factors which can affect wound healing 2

For Examiner

Q.1. Secondary intention 1


Q.2. keloid, hypertrophic scar, contracture. 1
Q.3. 2
i. Denervation
ii. Local infection
iii. Foreign body
iv. Decreased blood supply
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task

Carefully examine the given photograph and answer the following questions

Q.1.Identify the given medium (1)

Q.2.Which dye is added to this medium (1)

Q.3.Name the organisms growing on this medium (1)

Q.4.Describe the appearance of colonies on this medium. (1)

For Examiner
1.L.J medium 1
2.Malachite green 1
3.For mycobacterium 1
4 .Light brown granular colonies often called (Rough, buff, tough) 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

A 10-year-old boy is taken to a clinic because of fever and pharyngitis. A throat swab is plated
on blood agar plates and a bacitracin disk is placed on the plate, that showed beta-
hemolytic, and bacitracin- sensitive cocci. Gram stain of the etiologic agent is shown in a
photograph given below. Carefully examine picture and answer the following questions

Task

Q. 1.Give arrangement of bacteria in smear. (1)

Q.2.What is the likely organism. (1)

Q. 3. Which simplest microbiological test which can help the physician to determine the
genus of bacteria responsible for child’s illness. (1)

Q.4. Name two diseases caused by this organism. (1)

For Examiner
Q.1. Gram positive cocci in chains 1
Q.2.Streptococcus pyogenes 1
Q.3.catalase test 1
Q.4.Pharyngitis, Cellulitis, Impitigo, Necrotizing fasciitis 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: staining technique

For candidate:

A 65 years old man has a 7 months history of progressive weakness and weight loss of 1 kg
along with intermittent fever of chills and chronic cough productive of yellow sputum,
occasionally streaked with blood. Smear of his Sputum specimen is shown in a photograph
given below.

Task

Q.1.Examine the slide and give microscopic features. 02


Q.2 .What should be done to confirm the diagnosis in such patients? 01
Q.3. Name a counter stain used in this staining technique? 01

For Examiner

Q.1. Zn stain showing Acid Fast bacilli as bright pink rods 01

Q.2. Culture on Lowenstein Jenson (L J) Medium 01

Q.3.Malachite Green 01
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Biochemical reaction

For candidate:

Task

Q.1.Report the reaction observed in the provided TSI medium (01)

Q.2.What is the likely organism that would give these reaction (01)

Q.3. Name any two of the three sugars incorporated on this medium. (01)

Q.4. Which sugar is one tenth the concentration of other two sugars? (01)

For Examiner
Q1.Alkaline slant ,Acidic butt , H2S positive , Gas negative 1
Q.2.Salmonella Typhi 1
Q.3. Glucose, Lactose, Sucrose 1
Q.4. Glucose 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Instrument

For candidate:

Task

Q.1.Identify the instrument (1)

Q.2.Define sterilization (1)

Q.3. Give its principle. (1)

Q.3.Name 2 physical methods of sterilization (1)

For Examiner

Q.1. Autoclave 1
Q.2. The removal of all microorganisms and other pathogens from an object or surface by
treating it with chemicals or subjecting it to high heat or radiation. 1
Q.3. It is used to sterilize equipment subjecting them to high pressure saturated steam at
121 °C (249 °F) for around 15–20 minutes. 1
Q.4..Heat, Radiations . 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task

Q.1.Name the culture media . (1)

Q. 2.Name two organisms which can grow on this media. (1)

Q.3. Which type of media is this? (1)

Q.4. Define differential media. (1)

For Examiner

Q.1. Blood agar 1

Q.2. H.influenza, Streptococcus Pneumoniae ,Nisseriae meningitides 1

Q.3. Enriched and differential. 1

Q.4. It does not select against a group but allows the differentiation between 2 groups of
bacteria e.g., alphe,beta,&gamma hemolysis. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

TasK

1.Identify the medium (1)

2.Use of this medium. (1)

3.Name two fungi which can grow on this medium. (1)

4. How does it inhibit the groth of bacteria (1)

For Examiner

Q.1. Sabouraud’s Agar 1

Q.2. Growth of fungi 1

Q.3.Candida , Aspergillus 1

Q.4. Because of its low PH 1


AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Biochemical reaction

For candidate:

Task

Q.1.Report the reactions observed in provided urease medium . (1)

Q.2.Name the indicator used in this medium (1)

Q.3.Name two organisms which would give positive urease test (1)

Q.4. What is the principal of this test? (1)

For Examiner

Q.1. pink = positive urease test , yellow = Negative urease test 2


Q.2.Phenol red 1
Q.3. proteus species, klebsiella pneumoniae 1
Q.4.Urease enzyme attacks urea present in the medium and forms the alkaline end product
ammonia that causes the phenol red to turn deep pink. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task

Q.1.Identify the medium and growth (1)

Q.2.Name two organisms which give black colonies (1)

Q.3.Name single test to differentiate between two organisms (1)

Q.4. What type of media is this? (1)

For Examiner

Q.1.SS agar plate with H2s producing colonies 1


Q.2.Salmonella and Proteus 1
Q.3.Urease test 1
Q.4. Selective and differential. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Microbiology
Subject Material: Instrument
For candidate:
Task
Q.1.identify the instrument (1)
Q.2.Name the indicator used. (1)
Q.3.Name two organisms that require anaerobic growing envirnment for growth (1)
Q.4.What is the function of catalyst in this instrument? (1)

For Examiner
Q.1. Anaerobic Jar 1
Q.2. Methylene blue 1
Q.3.Clostridia , Bacteriodes 1
Q.4.A catalyst in the anaerobic jar permits oxygen to combine with hydrogen that comes
from a gas generator. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task Identify the picture and answer following questions:

Q.1.What type of hemolysis is seen (1)

Q.2.Name two organisms producing this hemolysis (1)

Q.3.Describe the colony morphology (1)

Q.4.4- what change in the colony morphology of this microorganism occurs after 24-48 hrs?
(1)

For Examiner
Q.1. alpha hemolysis 1
Q.2.Streptococcus pneumonia, streptococcus viridans 1
Q.3. Small,Colorless colonies,Round in shape,Having zone of alpha hemolysis and produce
green color around their colonies 1
Q.4.S. pneumoniae colonies have a flattened and depressed center after 24-48 hours of
growth on a bood agar plate. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task

Q.1.Identify the picture (1)

Q.2.Name the organism causing it. (1)

Q.3.which specimens can be obtained for its lab daignosis .any two.(2)

For Examiner

Q.1. sore throat 1


Q.2.streptococcus pyogenes 1
Q.3.Throat swab 1
Q.4.Blood/ pus 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Microbiology

Subject Material: Stainning

For candidate:

Task

A 6-year-old boy develops fever and headache. He is taken to the emergency room where he
is noted to have a stiff neck, suggesting meningeal irritation. A lumbar puncture is performed
and staining of CSF is shown in a photograph given below. Carefully observe the photograph
and answer the following questions :

Task

Q.1.Name the staining technique used 1


Q.2.Give the findings 1
Q.3. What is the likely organism involved in disease process . 1
Q.4. On which culture media, this organism can be isolated. 1

For Examiner

Q.1. Gram stainning 1


Q.2. Gram negative cocci in pairs intracellularly & extracellularly 1
Q.3. Nisseria meningitidis 1
Q.4. Chocolate agar 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task

Carefully examine the given sample material / photograph and answer the following
questions

Q.1.What are the use of these bottles. (1)

Q..2.Name the medium usually present in them. (1)

Q.3.How many cc of blood should be approximately inoculated in them. (1)

Q.4. How long does it take to get blood culture result. (1)

For Examiner
Q.1. Blood culture bottles 1
Q.2. Brain heart infusion 1
Q.3. 5-10 cc of blood 1
Q..4. A blood culture is a test to find an infection in the blood. Most bacteria can be seen in
the culture in 2 to 3 days, but some types can take 10 days or longer. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Microbiology

Subject Material: equipment

For candidate:

Task

Carefully examine the given instrument and answer the following question:

Q.1. Identify the following instrument . ( 1)

Q.2.What is it used for (1)

Q.3.What is done to this instrument before inoculation (1)

Q.4.What material the wire should be ideally made of (1)

For Examiner
Q.1. Inoculating loop 1
Q.2.Inoculation of specimens on culture media or making smears. 1
Q.3.Flammed for sterilization 1
Q. 4.Platinum 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser

Topic specification: Microbiology

Subject Material: Culture media and growth of microorganism

For candidate:

Task

You are shown a CLED culture plate . Carefully examine the given specimen and answer the
following question:

Q.1.what does CLED stands for? (1)

Q.2. Which indicator is used in this media. (1)

Q.3.Name two organisms which can grow on this medium. (1)

Q.4. Which specimen is inoculated on this media? (1)

For Examiner

Q.1. CLED stands for cystien lactose electrolyte deficient 1


Q.2.Bromothymol blue 1
Q.3.E.coli, Klebsiella . 1
Q.4. urine 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Parasitology
Subject Material: photograph
For candidate:
Task

A patient presented to the OPD with complaints of dull epigastric pain ,flatulence and
chronic diarrhea of steatorrhea type and stool voluminous ,foul smelling ,contains large
amount of mucus and fat but no blood. Stool examination show the following picture

Task

Q. 1. Which parasitic infection is the most likely cause? (1)


Q.2. What is the infective form of this parasite in man? (1)
Q.3. Draw & Lable the diagram of a trophozoite. (2)

For Examiner

Q.1.Giardia lamblia 1
Q.2.Mature cyst is the infective form . 1
Q.3. Pear-shaped with 2 nuclei, 4 pairs of flagella, and a suction disk with which it attaches to
the intestinal wall. 2
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Parasitology
Subject Material: photograph
For candidate:

Task

Q.1.identify the picture? (1)


Q. 2.Give its normal habitat. (1)
Q. 3. What is its mode of transmission. (1)
Q.4. What is the most common test for the diagnosis of this infection? (1)

For Examiner

Q.1.Trichomonas vaginalis 1
Q.2.Vagina/seminal vesicles ,urethra. 1
Q.3. By sexual contact 1
Q.4. In a wet mount of vaginal/prostatic secretions, the pear-shaped trophozoites have a
typical jerky motion. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organiser
Topic specification: Parasitology
Subject Material: photograph
For candidate

Identify the given slide of stool and answer the following questions

Task
Q.1. Name the organism. 1
Q.2 Give the identification points. 1
Q.3. What is the mode of transmission of this organism. 1
Q.4. To which class of parasites does it belong. 1

For Examiner

Q.1. Ascaris lumbricoides. 1


Q.2. Oval shaped ,thick albuminous coating, segmented ovum inside 1
Q.3. Feco oral route 1
Q.4. Intestinal Nematodes 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: Microbiology

Subject Material: parasitology

For candidate
Task
Carefully examine the given photograph and answer the following questions:
Q.1.Give your findings. 01
Q.2. Who is definitive host for Plasmodia. 01
Q.3.What is the stage that infects humans. 01
Q.4.Where does exo-erythrocyte stage occur in humans. 01

For Examiner
Q.1. Gametocytes of plasmodium falciparum 1
Q.2. Female Anopheles mosquito. 1
Q.3.Sporozoites in mosquito saliva. 1
Q.4. In the liver 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

For Organizer

Topic Specification: Microbiology/Parasitology

Subject Material: Ovum from the slide of the stool

For candidate:

A 35y old male presented in the OPD with the history off and on bloody diarrhea for last 2
months. .Stool examination reveals the following picture

Task
Q.1. Identify the ova and name the parasite. 1
Q.2.Give the morphology. 1
Q.3.What is the infective stage of this parasite. 1
Q.4.Name one complication produced by this parasite. 1

For Examiner

Q.1. Egg of trichuris trichura. 1


Q.2.Barrel shaped with mucus plug at both ends. 1
Q.3.Egg containing larva in the soil. 1
Q.4.Rectal prolapse. 1
AMNA INAYAT MEDICAL COLLEGE SHEIKHUPURA
GENERAL PATHOLOGY AND MICROBIOLOGY: (UNOBSERVED STATION)
Station no
Time allowed: 4min max marks: 04

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