Lab Practice 2 – Microorganism’s

culture
Lab practice 2 – Microorganism’s
culture
Materials YOU need to bring:
• Flavorless grenetine
• Powder broth (beef or chicken)
• Sugar
• 5 Gerber flasks with lid
• Plastic swabs
• Kitchen’s plastic

Lab materials
• 1 beaker of 250 mL
• 1 beaker of 500 mL
• 1 graduated cylinder of 250 mL
• 1 spatula
• 1 Bunsen burner
• 1 tripod
• 1 analytical balance
• Distilled water
 Lab practice 2 – Microorganism’s culture

Methodology
1. Dissolve 3.5 g of flavorless grenetine 9. Once solid, use the swabs to take
into 120 mL of distilled water at room samples of different surfaces and
temperature. inoculate each of the flasks with the
2. Mix well different samples, like in the video.
3. Add 20 g of sugar. 10. Close the flask with the lid and let it
4. Mix well. rest at room temperature for 3 to 4
5. Add 4 g of powder broth. days to let microorganisms grow.
6. Mix well. 11. Take pics but DO NOT OPEN THE
7. Pour into the Gerber Flask, ~ 20 mL FLASK EVER!
each. 12. Wait for next week’s lab practice to
8. Place the lid and wait for it to solidify, use a microscope and watch the
you can place it at 4°C. microorganisms with a microscope.
https://www.youtube.com/watch?v=hAVWmIf8No4 https://www.youtube.com/watch?v=uEXjot7vp7s
 Lab practice 2 - REPORT
I. Introduction:
• Short introduction about microorganisms, media and methods of culture.
II. Objectives:
• General objective:
 What, how and what is the purpose of the experiment?
• Specific objectives:
 The objective of each step of the experiment.
III. Materials and methods (materials used and each step of the process)
IV. Results
• Decribe your results according to the objectives.
• Describe the shape size, color and appearance of the microorganism’s colonies that grew in the media.
• Add images with descriptions.
V. Discussion
• Compare with other teams, describing both results. You can add pics of their results to support your
comparisons.
VI. Conclusion
• About your findings, NOT YOUR PERSONAL OPINION, this is NOT an essay.
VII. References
• The references where you obtained the information of the introduction, in APA format.
Lab practice 2 – Format of report
• All lab practices in TEAMS.
• Report in ENGLISH, one per team.
• For next week.
• In WORD.
• Add a cover page with name of the university, title of the
practice, your names, name of the subject, name of the teacher,
group and date.
• Do not copy!