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Cell Structure and Function Pretest: The Organelle Has A Phospholipid Membrane
Cell Structure and Function Pretest: The Organelle Has A Phospholipid Membrane
1. Researchers claimed that a particular organelle originated from a free-living prokaryotic cell that was
engulfed by a larger cell, as shown in Figure 1.
D The organelle has an internal aqueous environment that is similar to the cytosol of the larger cell.
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The illustration shows the active transport of hydrogen ions through a membrane protein.
2.
Which of the following best predicts the effect of not having available to supply energy to this
process?
C ions will continue to move through the protein in the original direction but at a slower rate.
D ions will begin to move through the phospholipid portion of the membrane in the original direction.
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3. Figure 1 shows a model of how a channel protein influences the movement of a particle across a cell’s
plasma membrane.
Figure 1. A section of a cell’s plasma membrane, showing a channel protein and a concentration gradient
across the membrane
An investigator wants to understand whether a newly found membrane protein is involved in membrane
transport of a certain particle. Which investigation will help determine whether the new membrane protein is
a channel protein involved in membrane transport?
Add small nonpolar molecules to the extracellular space and measure the direction of particle movement of
A
the molecules.
B Measure the rate of extracellular fluid movement into the intracellular space.
C Add more of the proteins to the plasma membrane and measure the rate of the particle movement.
Remove from the intracellular space and measure the rate of the particle movement into the
D
intracellular space.
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4.
Which statement best describes the effect on water transport across the cell membrane if the aquaporin in
the figure ceases to function?
A Water molecules will no longer be able to move across the cell membrane.
B Water molecules will still be able to move across the cell membrane but at a slower rate.
C Water molecules will only be able to enter the cell by active transport.
Water molecules will move across the cell membrane at a faster rate without the aquaporin regulating their
D
flow.
5. Aquaporins are channel proteins that facilitate the transport of water across the cell membrane. One group of
researchers hypothesizes that without functional aquaporins, no water will be able to enter the cell. A
different group proposes an alternative hypothesis, stating that even with nonfunctional aquaporins, a small
amount of water will still cross the cell membrane. An experiment is set up in which plant cells with
mutated (nonfunctional) aquaporins and plant cells with normally functioning aquaporins are both placed in
distilled water.
Which of the following data would support the alternative hypothesis?
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A Cells with functional aquaporins exhibit low turgor pressure and are hypertonic.
B Cells with functional aquaporins exhibit high turgor pressure and are hypotonic.
C Cells with mutated aquaporins exhibit an absence of turgor pressure and are completely plasmolyzed.
D Cells with mutated aquaporins exhibit moderate turgor pressure and are hypertonic.
6. Plant cell walls are composed of cellulose, while fungal cell walls are composed of chitin. A group of
scientists hypothesize that this difference means the cell wall has largely different functions in plant cells
and fungal cells. Alternatively, another group of scientists hypothesize that despite their biochemical
differences, plant and fungal cell walls serve similar functions.
Which of the following observations would best support the alternative hypothesis described above?
Plant cell walls are found just outside the plasma membrane, while fungal cell walls are found just beneath
A
the plasma membrane.
B In both plant cells and fungal cells, the cell wall surrounds the outside of the cell membrane.
C Some plant cells have secondary cell walls that confer additional rigidity, while fungal cells do not.
D Photosynthesis occurs in plant cells, but it does not occur in fungal cells.
7. Cholesterol is an important component of animal cell membranes. Cholesterol molecules are often delivered
to body cells by the blood, which transports the molecules in the form of cholesterol-protein complexes. The
complexes must be moved into the body cells before the cholesterol molecules can be incorporated into the
phospholipid bilayers of cell membranes.
Based on the information presented, which of the following is the most likely explanation for a buildup of
cholesterol molecules in the blood of an animal?
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8. Read each question carefully. Write your response in the space provided for each part of each question.
Answers must be written out in paragraph form. Outlines, bulleted lists, or diagrams alone are not
acceptable and will not be scored.
Paramecia are single‑celled aquatic eukaryotes that can survive in a wide range of salinities. Paramecia
use contractile vacuoles to help maintain homeostasis under these varying salt conditions. The
contractile vacuoles fill with fluid and then contract to remove the fluid from the paramecia.
Scientists wished to determine the effect of salinity on contractile vacuole activity. They maintained
different groups of paramecia in water of different salinities for one month. They then measured the
average amount of fluid ejected from the contractile vacuoles in each group of paramecia each time the
vacuoles contracted (Table 1).
10 (freshwater) 1.3
250 0.25
750 0.1
1000 0.05
(a) Describe how the phospholipids of a plasma membrane regulate the movement of large or polar
molecules across the membrane. Explain how osmosis will affect animal cells when the cells are placed
into an environment with a low water potential (high solute concentration) compared to the intracellular
water potential.
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(b) Use the template to construct an appropriately labeled graph that represents the data in Table 1.
(c) Describe how increasing salinity affects the amount of fluid ejected each time a contractile vacuole
contracts. Calculate the water potential ( ) of an animal cell without contractile vacuoles if water
enters the cell and creates a solute potential ( ) of . Assume that the pressure potential ( ) in the
cell is 0.
Part A (i)
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Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1
The response indicates that the hydrophobic tails of the phospholipid fatty acids block the movement of large and
polar molecules, which require protein channels to cross the membrane.
Part A (ii)
Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1
The response indicates that osmosis will cause an overall movement of water out of the cells and that the cells will
shrink.
Part B
Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1 2 3
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Part C (i)
Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1
The response indicates that the amount of fluid ejected decreases with increasing solute concentration. There is a
large change in ejection volume when comparing paramecia in freshwater to paramecia in 250 milliosmoles of
. With paramecia in higher salinities, the amount of fluid ejected continues to decrease as salinity
increases.
Part C (ii)
Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1
The response indicates that , because pressure potential solute potential ( )equals water
potential ( ) .
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9. Water is constantly diffusing into the cytosol of freshwater single-celled organisms. In order to maintain the
proper solute concentrations in the cytosol, contractile vacuoles pump out the excess water. An
experimenter placed single-celled organisms into various saline concentrations and recorded the used
by the contractile vacuole. The data are shown in the graph.
Of the following, which additional investigation can be used to determine when the cells are in an isotonic
solution?
A Decreasing the salinity of the environment a little at a time until the usage reaches a maximum
B Decreasing the salinity of the environment a little at a time until usage reaches a minimum
C Increasing the salinity of the environment a little at a time until usage reaches a maximum
D Increasing the salinity of the environment a little at a time until the usage reaches a minimum
10. Which of the following statements best supports the claim that certain organelles within eukaryotic cells
evolved from free-living prokaryotic cells?
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Eukaryotes and prokaryotes both contain ribosomes, but the ribosomes of eukaryotes are more complex in
B
structure than those of prokaryotes.
Eukaryotes exchange segments of internal membranes between the endoplasmic reticulum and Golgi
C
apparatus, but prokaryotes have no such internal membranes.
Some organelles contain their own that is more similar to prokaryotic in structure and function
D
than to the eukaryotic found in the cell's nucleus.
11. Organelles such as mitochondria and the endoplasmic reticulum have membranes that compartmentalize
reactions and other metabolic processes. To function properly, the organelles must move substances across
their membranes.
Which of the following statements describes a feature shared by mitochondria and the endoplasmic
reticulum that increases the efficiency of their basic functions?
D They have double membranes, with one membrane enclosed within the other.
12. All eukaryotic cells contain at least one Golgi complex, typically located in the cytoplasm and near the
endoplasmic reticulum.
Which of the following best describes a process that occurs within the Golgi complex?
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13. Changing the shape or morphology of the mitochondrial inner membrane can change the efficiency of
mitochondrial function.
Which of the following outcomes will most likely result from a change in the shape of the mitochondrial
inner membrane from a highly folded surface to a smooth, flat surface?
Mitochondria will become more efficient because the inner mitochondrial membrane will become more
A
permeable to ions.
B Mitochondria will become more efficient because the total volume of the mitochondria will increase.
Mitochondria will become less efficient because the inner mitochondrial membrane will become less
C
permeable to large molecules.
Mitochondria will become less efficient because the surface area of the inner mitochondrial membranes will
D
decrease.
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14. The figure shows a process by which a cell might absorb food from its surrounding environment and break
it down for use as a source of energy and matter. The process involves lysosomes, which are membrane-
bound organelles that contain hydrolytic enzymes. Activation of the hydrolytic enzymes requires an acidic
, and lysosomes maintain an internal acidic by using ion pumps.
Which of the following outcomes will most likely result from a loss of ion pump function in the cell’s
lysosomes?
The internal of the lysosomes will decrease, which will prevent the activation of hydrolytic enzymes and
A
interfere with the intracellular digestion of food.
The internal of the lysosomes will increase, which will prevent the activation of hydrolytic enzymes and
B
interfere with the intracellular digestion of food.
The internal of the lysosomes will decrease, which will activate hydrolytic enzymes and enhance the
C
intracellular digestion of food.
The internal of the lysosomes will increase, which will activate hydrolytic enzymes and enhance the
D
intracellular digestion of food.
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15. Which of the following claims is scientifically accurate and consistent with an observation that a decrease in
lysosome production within a cell leads to a decline in mitochondrial activity?
A A lack of lysosomes will cause a decrease in the synthesis of enzymes necessary for cellular respiration.
Fewer lysosomes will be available to break down macromolecules to provide the necessary nutrients for
B
cellular respiration.
C Fewer lysosomes will be available to store materials required for the functioning of the mitochondria.
D Lysosomes will not be available to modify proteins so that they are targeted to the mitochondria.
A cell’s membrane potential is maintained by the movement of ions into and out of the cell. A model
16.
showing the influence of membrane proteins on the movement of sodium ( ) and potassium ( ) ions
across the plasma membrane is presented in Figure 1.
Figure 1. Section of a cell’s plasma membrane, showing ion concentrations and membrane proteins
Based on the model presented in Figure 1, which of the following outcomes will most likely result from a
loss of protein function?
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A The membrane potential will be disrupted by an increase in concentration inside the cell.
B The membrane potential will be disrupted by an increase in concentration inside the cell.
C The membrane potential will be maintained by the pump moving more ions into the cell.
D The membrane potential will be maintained by the diffusion of ions into the cell.
17. The endosymbiont theory proposes a model for the evolution of mitochondria. According to the model, an
ancestral eukaryote engulfed a small, free-living prokaryotic organism. The engulfed prokaryote then
formed an endosymbiotic relationship with the eukaryotic host.
Which of the following observations best supports the model?
Organelles such as mitochondria and the endoplasmic reticulum have membranes composed of
B
phospholipids.
C Mitochondria and some prokaryotes share similar metabolic reactions that produce .
18. Which of the following statements best predicts the effect of increasing the permeability of the
mitochondrial membranes to large molecules?
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production will increase because of an increase in the rate at which proteins diffuse out of
A
mitochondria.
production will increase because of an increase in the mixing of mitochondrial and cytosolic
B
substances.
C production will decrease because of an increase in the occurrence of uncontrolled chemical reactions.
D production will decrease because of an increase in the surface area of the mitochondrial membranes.
Muscle cells have high demands. Which of the following is a scientific claim about how the structure
19.
of the mitochondria in muscle cells should be different than it is in other cells because of the high energy
demands of mitochondria?
The inner membrane of the mitochondria in muscle cells should have more folds to increase the surface area,
A
allowing more to be synthesized.
The inner membrane of the mitochondria in muscle cells should be more permeable to large enzymes,
B
allowing the same reactions to occur in both compartments of the mitochondria.
The outer membrane of the mitochondria in muscle cells should be thicker, allowing more rapid diffusion of
C
molecules into the mitochondria.
The outer membrane of the mitochondria of muscle cells should have more folds, increasing the surface area
D
for faster diffusion of molecules from the cytoplasm.
20. A team of biologists develop a new drug, and one team member hypothesizes that the drug is incapable of
freely passing across the plasma membrane and requires the help of membrane proteins to enter cells.
Alternatively, another biologist on the team hypothesizes that the drug can diffuse passively across the
plasma membrane like and can.
Which of the following, if true about the drug, best supports the alternative hypothesis that the new drug
will exhibit simple diffusion across plasma membranes?
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21. Which of the following statements best explains the processes of passive and active transport?
Passive transport is the net movement of substances down a concentration gradient that requires metabolic
A energy. Active transport is the movement of substances up a concentration gradient that does not require
energy.
Passive transport is the net movement of substances down a concentration gradient that does not require
B metabolic energy. Active transport is the movement of substances up a concentration gradient that requires
energy.
Passive transport is the net movement of substances up a concentration gradient that requires metabolic
C energy. Active transport is the movement of substances down a concentration gradient that does not require
metabolic energy.
Passive transport is the net movement of substances up a concentration gradient that does not require
D metabolic energy. Active transport is the movement of substances down a concentration gradient that
requires energy.
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22.
A Areas 1 and 3 are polar, since the membrane molecules are aligned with water molecules.
B Area 2 is polar, since water has been excluded from this area of the membrane.
C Areas 1 and 3 are hydrophilic, since membrane molecules formed covalent bonds with water.
D Area 2 is nonpolar, since hydrogen bonds between the adjacent lipids hold the membrane together.
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23. The model below shows the structure of a portion of a plasma membrane in an animal cell.
Which statement best explains the orientation of the phospholipid molecules in this model?
A The nonpolar portions of the phospholipid molecules are attracted to the internal and external environments.
The hydrophilic phosphate groups of the phospholipid molecules are attracted to the aqueous internal and
B
external environments.
The embedded proteins attract the fatty acid tails of the phospholipids, so the tails point away from the
C
internal and external aqueous environments.
The fatty acid tails of the phospholipid molecules are hydrophilic and are repelled by the internal and
D
external aqueous environments.
24. Researchers propose a model to explain variation in phytoplankton cell sizes in a marine environment. They
base their model on the idea that smaller cells absorb nutrients more efficiently. The researchers predict that
the mean diameter of phytoplankton cells will change by 50 micrometers for every 5-kilometer increase in
distance from the shore because of a gradual decrease in nutrient availability. To test their model, the
researchers determine that the phytoplankton cells found closest to shore have a mean diameter of 900
micrometers.
Based on the model, what will be the mean diameter of the phytoplankton cells that are found 25 kilometers
from shore?
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A 650 micrometers
B 875 micrometers
C 925 micrometers
D 1150 micrometers
25. In an experiment, cells were isolated from an aquatic plant and suspended in pond water, a sucrose sugar
solution, or distilled water. All of the cells were then viewed under a microscope. Compared with the cell in
the pond water, the cell in the sugar solution appeared shriveled, and the cell in the distilled water appeared
inflated. The results of the experiment are represented in Figure 1.
There was a net movement of sucrose out of the cell suspended in the sugar solution and a net movement of
A
sucrose into the cell suspended in the distilled water.
There was a net movement of sucrose into the cell suspended in the sugar solution and a net movement of
B
sucrose out of the cell suspended in the distilled water.
There was a net movement of water out of the cell suspended in the sugar solution and a net movement of
C
water into the cell suspended in the distilled water.
There was a net movement of water into the cell suspended in the sugar solution and a net movement of
D
water out of the cell suspended in the distilled water.
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26. The transport of a substance across a plasma membrane of a specific organelle requires energy. The rate at
which the transport takes place also depends on temperature. A scientist isolated the specific organelle and
then used the following treatments to determine the conditions that will result in the maximal transport. All
treatments contained the extracted organelle and were maintained at .
The data from this experiment indicate that maximal rate of transport of protein at occurs at an
concentration of .
Incubate samples containing of at and determine the rate of transport for four other
D
proteins.
Cells contain smaller components called organelles that are necessary for a cell’s survival. Organelle
27.
functions have often been compared to components of larger systems.
Which of the following functional differences between the rough and smooth endoplasmic reticulum
is explained by the structural differences between them?
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A Rough breaks down toxic substances, and smooth only transports them out of the cell.
B Rough can synthesize and package lipids for export, and smooth cannot.
D Rough can synthesize and package proteins for export, and smooth cannot.
28. A spherical bacterial cell has a radius of . The human egg cell has a radius of .
Which statement correctly indicates the cell that is able to more efficiently exchange materials with the
external environment and provides a correct explanation?
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29. Two different models of a living cell are represented in the figure.
Of the two cells represented in the figure, which would likely be more efficient at exchanging substances
with the surrounding environment?
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30. A model of the plasma membrane showing several biological molecules, including a transmembrane
protein, is shown in Figure 1.
A Interactions of the hydrophobic and hydrophilic amino acids help to anchor the protein in the membrane.
Interactions of the peptide bonds of the protein with the membrane will affect the rate at which substances
B
can cross the membrane.
Interactions of the quaternary structure of the protein will increase hydrogen bonding in the membrane and
D
make the membrane less fluid.
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31. Researchers have proposed a model of the process by which a newly synthesized protein is transported to
the plasma membrane and secreted into the extracellular space. The model is represented in Figure 1.
A The nucleus
32. Read each question carefully. Write your response in the space provided for each part of each
question. Answers must be written out in paragraph form. Outlines, bulleted lists, or diagrams
alone are not acceptable and will not be scored.
Mitochondria and chloroplasts are the energy-processing organelles of eukaryotic cells. Both organelles
contain outer and inner membranes. The inner membrane of the mitochondrion contains many folds.
The chloroplasts have a third membrane forming the thylakoids. Molecules such as enzymes
responsible for the synthesis of ATP are embedded in the inner membrane of the mitochondrion and the
thylakoid membrane of the chloroplast.
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(a) Describe the importance of the inner membranes separating different regions of the mitochondrion
and the chloroplast.
The inner membranes allow the organelles to compartmentalize because of
increased folds, therefore, increasing surface area and efficiency.
Please respond on separate paper, following directions from your teacher.
(b) Based on the diagram, describe how the structure of these organelles controls movement across the
membranes.
The molecules would first pass through the first membrane and stop, or continue
to pass through the second and third membrane.
Please respond on separate paper, following directions from your teacher.
(c) Complete the table to represent the relationship between volume and surface area of a spherical
structure surrounded by a membrane.
1: 1
1: 3
(d) Explain how the folding of the mitochondrial inner membrane and the many individual chloroplast
thylakoids are beneficial to a cell that is carrying out chemical reactions.
The folding of the membrane increases the surface area, as does the thylakoids.
With more surface area, more reactions are able to take place.
Please respond on separate paper, following directions from your teacher.
Part A
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Select a point value to view scoring criteria, solutions, and/or examples and to score the response
0 1
The response indicates that the inner membranes permit different reactions to occur in different regions of the
organelles.
Part B
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0 1
Part C
Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1
The response shows a completed table with the correct values in the Volume, Surface Area, and Ratio of Volume to
Surface Area columns.
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Part D
Select a point value to view scoring criteria, solutions, and/or examples and to score the response.
0 1
The response indicates that the folding increases surface area (as in part c) so that the membrane can hold more
enzymes, and more reactions (e.g., production) can take place than if less surface area was present).
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