Primer Information

Micro SSP™ Seramates HLA Class I DNA Typing Tray - B5, Lot #002
*SSPSB5_002C02*
Cat. ID# SSPSB5

Notes
1 Betaglobin nucleotide position based on Fullerton, S.M., et al. Proc. Natl. Acad. Sci. U.S.A. 91 (5), 1805-1809 (1994).
2 The QC summary of each primer mix is located in the shaded area. This information reflects the data available upon the initial release. For the QC information on a specific
dotting please contact One Lambda, Inc.

3 The allele nomenclature includes the HLA alleles listed in the January 2021 update of the IMGT/HLA database release 3.43.0 (Robinson J, et al., "IMGT/HLA and IMGT/MHC:
sequence databases for the study of the major histocompatibility complex" Nucleic Acids Research (2003), 31, 311-314). Complete update information is located at
www.ebi.ac.uk/imgt/hla/.
4 Revision summary: January 2021 nomenclature update based on IMGT/HLA 3.43.0.
5 (2F/4F/6F/8F/10F/12F) Bw6 (w/o B*07:02:67; B*07:13; B*07:97; B*07:110; B*07:179; B*07:192; B*07:195; B*07:244; B*07:257; B*07:412; B*08:29; B*08:36; B*08:97; B*08:101;
B*08:114; B*08:131; B*08:136; B*08:139; B*14:01:05; B*14:02:05; B*14:02:08; B*14:30; B*14:34; B*15:01:25; B*15:01:27; B*15:01:49; B*15:277; B*15:311; B*15:354; B*15:430;
B*15:434; B*15:521; B*18:01:21; B*18:01:24; B*18:45; B*18:99; B*18:133; B*18:162; B*18:179; B*35:01:39; B*35:01:52; B*35:02:03; B*35:02:14; B*35:17:02; B*35:152;
B*35:222; B*35:248; B*35:293; B*35:501; B*39:01:15; B*39:24:02; B*39:100; B*39:104; B*39:105; B*39:115; B*40:01:31; B*40:02:22; B*40:02:29; B*40:76; B*40:127; B*40:135;
B*40:193; B*40:212; B*40:217; B*40:218; B*40:223; B*40:281; B*40:306; B*40:311; B*40:327; B*40:335; B*40:400; B*40:421Q; B*40:423; B*40:436; B*41:02:02; B*41:05;
B*41:26; B*41:55; B*42:22; B*45:01:05; B*45:01:09; B*45:16; B*45:21; B*46:48; B*46:75; B*46:77; B*46:86; B*48:20; B*48:36; B*50:38; B*50:46; B*50:53; B*54:01:01:01;
B*54:01:01:02; B*54:01:01:03; B*54:01:01:04; B*54:01:01:05; B*54:01:02; B*54:01:03; B*54:01:04; B*54:01:05; B*54:01:06; B*54:01:07; B*54:01:08; B*54:01:09; B*54:02;
B*54:03; B*54:04; B*54:06; B*54:07; B*54:09; B*54:10; B*54:11; B*54:13; B*54:14; B*54:15; B*54:16; B*54:17; B*54:18; B*54:19; B*54:20; B*54:21; B*54:22; B*54:23; B*54:24;
B*54:25; B*54:26; B*54:27; B*54:28; B*54:29; B*54:30; B*54:31; B*54:32; B*54:33; B*54:34; B*54:35; B*54:36; B*54:37; B*54:38; B*54:39; B*54:40; B*54:41; B*54:42; B*54:43;
B*55:01:13; B*55:73; B*55:88; B*67:02:01:01; B*67:02:01:02; B*81:06):
B*07:02:01:01~02:66/02:68~12/14:01~26/28~35/37:01~37:02/39~80/82~96:02/98~109/111N~148/150~178/181N~191/193~194/196~218/220~235/237~243/245~256/258~272N
/274~411/413~415N;
B*08:01:01:01~01:56/04:01~28/30N~35/37~51/53:01~66/68~77/79~96:02/98~100/102~113/115~116/118~125/127~130/132~135/137~138/140~254/256~266; B*13:09/39/67;
B*14:01:01:01~01:04/01:06~02:04/02:06~02:07/02:09~29/31~33/35~92/94~99;
B*15:01:01:01~01:24/01:26/01:28~01:48/01:50~12:03/14~15:01:02/18:01:01~21:01:02/25:01:01~35/37~42/44~66/68~86/88/90~94N/96~114/116~156/158~161/163~167/169~17
6/178~195/197:01~207/209N~215/217~221/223~229/231~253/255/257~267/269~272N/274~276/278~310/312~338/340~353/355/357~360/363:01~395/397~402/404~407/409~
410/412~417/419~422/425~429/431~433/435~441/443~445/447~461/463N~499/501~504/506~515/517~520Q/522/524~531/533~545/547~549N/551~554/556~574/576~585;
B*18:01:01:01~01:20/01:22~01:23/01:25~08/10~44:02/46~53/55~66/68~98:02/100~132/134~135:01:02/137~161/163~178/180~193/196~201;
B*27:08/12:01:01~12:01:03/18/26/33/40/42/44/89/153/204;
B*35:01:01:01~01:38/01:40~01:51/01:53~02:02/02:04~02:13/02:15~17:01/17:03~151:02/153~172/174~221/223~247/249~292/294~328/330~470/472~500/502~507;
B*37:05/11/37/67; B*38:156~159; B*39:01:01:01~01:14/01:16~24:01/25N~99/101~103/106~114/116N~170;
B*40:01:01~01:30/01:32~02:21/02:23~02:28/02:30~12:02/14:01~18/20:01:01~46/48~75/77~95/97~108/111~116/118N~126/128~134/136~156/158~187/189~192/194~200/202~
211/213~216N/219~222/224~280/282~291N/293~305/307~310/312~326/328~334/336~339/341~393/395~399N/401~420/422/424~425/427~435/437~475;
B*41:01:01:01~02:01:09/02:03~04/06~25/27~45N/47~54/56Q~59/61~68; B*42:01:01:01~21/23~28; B*44:09/46:01~46:02/75/90/129/131/254/277;
B*45:01:01:01~01:04/01:06~01:08/01:10~15/17~20/22~27; B*46:01:01:01~47/49~74/76/78~85/87; B*47:02; B*48:01:01:01~17/19/21~35/37~50;
B*50:01:01:01~37/39~45/47~52/54~74; B*55:01:01:01~01:12/01:14~72/74~87/89N/91~102/104~111; B*56:01:01:01~06/08~20:02/22~45/47~64/66~77N; B*57:12; B*58:64;
B*67:01:01~01:03/03~07; B*73:01:01:01~03; B*78:01:01:02~10; B*81:01:01:01~05/07~10; B*82:01:01:01~04; B*83:01

4 (2F/4F/6F/8F/10F/12F) Changes: B*07:07:01=B*07:07:01:01, B*07:31=B*07:31:01, B*08:213=B*08:213:01, B*35:51=B*35:51:01, B*35:205=B*35:205:01,

B*35:215=B*35:215:01, B*35:460=B*35:460Q, B*40:01:03=B*40:01:03:01, B*40:245=B*40:245:01, B*40:353:01=B*40:353:01:01, B*81:02=B*81:02:01
New: B*07:02:01:70, B*07:02:01:71, B*07:02:01:72, B*07:02:01:73, B*07:02:01:74, B*07:02:01:75, B*07:02:01:76, B*07:02:01:77, B*07:02:01:78, B*07:02:01:79, B*07:02:01:80,
B*07:02:82, B*07:02:83, B*07:02:84, B*07:07:01:02, B*07:31:02, B*07:408, B*07:409, B*07:410, B*07:411, B*07:413, B*07:414, B*07:415N, B*08:01:01:39, B*08:01:01:40,
B*08:01:01:41, B*08:01:01:42, B*08:01:01:43, B*08:01:01:44, B*08:01:01:45, B*08:213:02, B*08:263, B*08:264, B*08:265, B*08:266, B*14:01:11, B*14:02:01:20, B*14:02:27,
B*14:02:28, B*14:91, B*14:92, B*14:94, B*14:95, B*14:96, B*14:97, B*14:98, B*14:99, B*15:01:01:39Q, B*15:01:01:40, B*15:01:01:41, B*15:01:64, B*15:01:65, B*15:01:66,
B*15:01:67, B*15:02:01:05, B*15:10:06, B*15:10:07, B*15:18:01:09, B*15:569, B*15:570, B*15:571, B*15:572, B*15:573, B*15:574, B*15:576, B*15:577, B*15:578, B*15:579,
B*15:580, B*15:581, B*15:582, B*15:583, B*15:584N, B*15:585, B*18:01:01:52, B*18:01:01:53, B*18:01:01:54, B*18:01:33:03, B*18:01:42, B*18:01:43, B*18:01:44,
B*18:03:01:03, B*18:07:03, B*18:196, B*18:197, B*18:198, B*18:199, B*18:200, B*18:201, B*35:01:01:51, B*35:01:01:52, B*35:01:01:53, B*35:01:01:54, B*35:01:01:55,
B*35:01:01:56, B*35:01:01:57, B*35:02:01:06, B*35:03:32, B*35:05:05, B*35:08:01:09, B*35:08:01:10, B*35:08:14, B*35:29:03, B*35:51:02, B*35:205:02, B*35:215:02, B*35:490,
B*35:491, B*35:492, B*35:493, B*35:494, B*35:495, B*35:496, B*35:497, B*35:498, B*35:499, B*35:500, B*35:502, B*35:503, B*35:504, B*35:505, B*35:506, B*35:507,
B*39:01:01:15, B*39:01:01:16, B*39:01:01:17, B*39:05:01:05, B*39:05:04, B*39:09:05, B*39:169, B*39:170, B*40:01:02:35, B*40:01:02:36, B*40:01:02:37, B*40:01:02:38,
B*40:01:02:39, B*40:01:02:40, B*40:01:02:41, B*40:01:03:02, B*40:02:01:19, B*40:02:32, B*40:02:33, B*40:03:01:03, B*40:06:01:13, B*40:06:01:14, B*40:245:02,
B*40:353:01:02, B*40:463, B*40:464, B*40:465, B*40:466, B*40:467, B*40:468, B*40:469, B*40:470, B*40:471, B*40:472, B*40:473, B*40:474, B*40:475, B*41:01:01:05,
B*41:02:01:09, B*41:67, B*41:68, B*45:01:11, B*45:27, B*46:01:01:05, B*46:01:30, B*46:82, B*46:83, B*46:84, B*46:85, B*46:87, B*50:01:01:07, B*50:01:17, B*50:71,
B*50:72N, B*50:73, B*50:74, B*55:110, B*55:111, B*56:01:01:13, B*56:01:01:14, B*56:01:01:15, B*56:01:16, B*56:01:17, B*56:75, B*56:76, B*56:77N, B*73:03, B*81:02:02

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 1 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 6 (2G/4G/6G/8G/10G/12G) Bw4 (w/o B*13:28; B*13:70; B*15:403; B*15:481; B*27:05:25; B*27:05:37; B*27:49; B*27:116; B*27:122; B*27:156; B*27:169; B*27:178; B*27:221;
B*37:24; B*37:58; B*37:59; B*37:64; B*37:90; B*38:60; B*38:61; B*38:76; B*38:169; B*44:03:16; B*44:03:30; B*44:111; B*44:127; B*44:145; B*44:172; B*44:200; B*44:255;
B*44:458; B*44:495; B*44:504; B*49:01:18; B*49:21; B*51:01:14; B*51:12; B*51:67; B*51:100; B*51:159; B*51:171; B*51:202; B*51:205; B*51:239; B*51:266; B*51:291;
B*51:314; B*51:315; B*52:01:19; B*52:01:42; B*52:04; B*52:34; B*52:35; B*53:01:22; B*53:10; B*53:52; B*57:01:08; B*57:01:29; B*57:08; B*57:34; B*57:105; B*57:114;
B*57:117; B*57:126; B*58:01:34; B*58:42; B*58:54; B*58:70; B*58:81; B*58:125):
B*07:27/36/38/81/149/180/219/236/273; B*08:02~03/52/78/117/126/255; B*13:01:01:01~08/10~27/29~38/40~66/68~69/71~151; B*14:93;
B*15:13:01~13:03/16:01:01~17:07/23~24:02/36/43/67/87/89:01~89:02/95/115/157/162/168/177/196/208/216/222/230/254/256/268/273/339/356/361~362/396/408/411/418/423~
424/442/446/462/500/505/516/523/532/546Q/550/555/575N; B*18:09/54/67/136/194:01:01~195;
B*27:01~05:24/05:26~05:36/05:38~07:06/09~11/13:01~17/19:01:01~25/27~32/34~39/41/43/45~48/50:01~88/90:01~115/117~121/123~152/154~155/157~168/170~177/179~203
/205~220/222~238; B*35:329/471; B*37:01:01:01~04:02/06:01~10/12~23/25~36/38~57/60~63/65~66/68~81/83~89/91~95;
B*38:01:01:01~59/62~75/77~86/160~164/166~168/170~171; B*40:13/19/47/96/109~110/117/157/188/201/292/340/394; B*41:46/60;
B*44:02:01:01~03:15/03:17~03:29/03:31~08/10~45/47~74/76~89/91~110/112~126:02/128:01~128:02/130/132~144/146~171N/173~199/201~253/256~276/278~344/346~437/43
9~457/459~494/496~503; B*47:01:01:02~01:05/03~10; B*48:18; B*49:01:01:01~01:17/02~20/22~70;
B*51:01:01:01~01:13/01:15~11N/13:01~66/68~99/101~109/111~158:02/160~170/172~201/203~204:02/206~238/240~265/267~290/292~313N/316~332;
B*52:01:01:01~01:18/01:20~01:41/01:43~03/05~33/36~101; B*53:01:01:01~01:21/01:23~09/11~51/53~63; B*54:12; B*55:90/103; B*56:07/21/46/65;
B*57:01:01:01~01:07/01:09~01:28/01:30~07/09~11/13~33/35~104/106~113/115~116/118~125/127~141;
B*58:01:01:01~01:33/01:35~41/43~53/55~63/65~69/71~80/82~124/126~127; B*59:01:01:01~12

7 Allele sequences are obtained from the IMGT database. Updates, including modifications to allele sequences may alter bead specificity.
8 Micro SSP™ Seramates is intended for use with One Lambda, Inc. serological typing products.
9 Not all alleles were directly tested. Untested alleles were predicted and, therefore, may cause false reactions. Refer to 'Nomenclature List' for the list of tested alleles.

10 The allele specificity for some wells include alleles with unsequenced regions at the target site. (Sequence information is available at www.ebi.ac.uk/imgt/hla/.) For these alleles,
the published sequence from the most closely related allele is used as reference for the unsequenced regions. The reference allele provides the most likely prediction of the
unsequenced regions. There may be a disparity between the predicted and newly sequenced information, which may alter the allele specificity of a well. Allele specificity
information will be updated as new sequence information is available.

11 The Prev. Lot column lists the location of primers that existed on a previous lot of the product. Revision changes to primer specificity are noted in the Specificity Changes column.

12 This allele specific product is not intended for the typing of other allele groups.
13 The following alleles are not included in this kit: None

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 2 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

Negative Control (betaglobin)

H 525(-)549 1248(-)1267 750 - 0 0 0 0 0 NA

B*08:09/84; B*13:46/53/131; B*15:42/

83:01:01~83:01:02/485; B*35:60/276;
B*39:06:01~06:06/34/62/64/83:01~
83:03/90/109/127/129/132/140~141/
154; B*41:66; B*42:04; B*46:18;
B*51:21/36/101/143/153/157/197/280/
295/300; B*52:37/71/84;
B*54:01:01:01~02/04~05N/07~08N/10~ 95W-T97 163T163 225
25/27~32/34~37/39/41~43;
B*55:01:01:01~03/05~07/10/12~13/15~
21/23/25~48/50/52~85/87~109/111;
B*56:23/54; B*59:01:01:01~03/05~08/
10N~11; B*78:07; C*07:758; C*12:183;
C*14:92
Changes:
B*51:104=B*51:104:01,
B*51:232=B*51:232:01
New: B*40:06:01:13,
B*40:06:01:14, B*40:464,
1 B*40:468, B*40:473,
B*07:84; B*08:09/84; B*13:35/59/62; B*51:01:01:65, B*51:01:01:66,
3 B*51:01:01:67, B*51:01:01:68,
B*15:83:01:01~83:01:02; B*27:130/
5 153; B*35:273; B*40:06:01:01~06:08/
B*51:01:01:69, B*51:01:01:70,
B*51:01:85, B*51:01:86,
7 G 06:10~06:17/06:19~06:25/44/53/ - 96 19 0 0 77 1 B*51:01:87, B*51:02:01:03,
70:01~70:02/75/83/93/95~96/103/109~ B*51:08:01:03, B*51:08:01:04,
9 110/127/131/148/161~162/165/167/ B*51:104:02, B*51:232:02,
11 177/190/230/244/256N/268~270/275/ B*51:322, B*51:323, B*51:324,
294~295/298:01~298:02/306~307/311/ B*51:325N, B*51:326, B*51:327,
313/317~318/340~342/348~350/361N~ B*51:328, B*51:329Q, B*51:330,
B*51:331, B*51:332,
364/373~374/376/387~389/408/411/
B*52:01:01:17, B*52:101,
423/425/427/434/445/449/451/453~ B*54:01:01:03, B*54:01:01:04,
454/459~460/464/468/473; B*41:66; B*54:01:01:05, B*54:42,
B*42:04; B*44:459; B*51:01:01:01~ B*54:43, B*55:111, B*59:11
01:47/01:49~01:71/01:73~03/05/
07:01~12/14/16~34/36/38~41N/43~44N/ 95W-T97 116Y116 100
48~55/57~58/60~61:02/65~80/82~84/
86~91/93~96/98N~105/107~134/136~
138/140~147/149N~151/153~156/
158:01~171/173Q~185/187~191/193/
195~213/215/217~219/222/224/226/
228~250/252~260/262~273N/275~280/
282~292/294~299/301~309/311~318N/
320~332; B*52:01:01:01~13/15~24/27~
38/40~57/59~74/76~87/89N~96N/98~
101; B*54:04/11/15/42; B*55:17/20/27~
28:02/81; B*56:05:01~06/15/21;
B*58:08:01~08:02; B*59:02/11;
B*78:01:01:02~10; C*03:464; C*14:92

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 3 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

Changes:
B*07:38; B*08:03/52/78; B*18:136; B*51:104=B*51:104:01,
B*51:232=B*51:232:01
B*35:471; B*38:06~07/166; B*44:06;
New: B*51:01:01:65,
B*49:67; B*51:01:01:01~01:07/01:09~
B*51:01:01:66, B*51:01:01:67,
01:10/01:12~01:16/01:18~01:39/ B*51:01:01:68, B*51:01:01:69,
01:41~01:42/01:44~06:04/08:01:01~ B*51:01:01:70, B*51:01:85,
21/23~43/45~49/51~53/55~77/79~117/ B*51:01:86, B*51:01:87,
F 119~159/161~213/215~221/223~ 63N-QIF67 80I80 75 - 96 11 0 0 85 1 B*51:02:01:03, B*51:08:01:03,
230:01:03/232:01~244/246~250/252~ B*51:08:01:04, B*51:104:02,
309/311~332; B*53:01:01:01~01:17/ B*51:232:02, B*51:322,
B*51:323, B*51:324, B*51:325N,
01:19~02/04~08:02/10/14~16/18~27/
B*51:326, B*51:327, B*51:328,
29~30/32~35/37/39/41~54/56~63; B*51:329Q, B*51:330, B*51:331,
B*59:01:01:01~07/09~12 B*51:332, B*53:01:01:11,
B*53:01:26, B*53:63, B*59:11,
1 B*59:12
3
5
Changes:
7 B*18:194=B*18:194:01:01,
9 B*18:67/136/194:01:01~194:01:02;
B*51:232=B*51:232:01
New: B*18:194:01:02, B*27:236,
11 B*27:236; B*35:471; B*37:34; B*38:06~ B*51:01:01:65, B*51:01:01:66,
1 07; B*44:06; B*49:03; B*51:01:01:01~ B*51:01:01:67, B*51:01:01:68,
01:07/01:09~01:39/01:41~01:42/ B*51:01:01:69, B*51:01:01:70,
3 01:44~49/51~53/55~66/68~77/79~103/ B*51:01:85, B*51:01:86,
B*51:01:87, B*51:02:01:03,
5 105~111/113~146/148~266/268~283/
B*51:08:01:03, B*51:08:01:04,
7 E 285~332; B*52:01:01:01~01:28/01:30~ 45T45 80I80 150 - 95 18 0 0 77 1
B*51:232:02, B*51:322,
01:48/03~15/17~19/21:01~31:02/33~ B*51:323, B*51:324, B*51:325N,
9 101; B*53:01:01:01~01:17/01:19~02/ B*51:326, B*51:327, B*51:328,
11 04~08:02/14~29/32~35/37/40~54/56~ B*51:329Q, B*51:330, B*51:331,
63; B*57:120; B*58:01:01:01~35/37~43/ B*51:332, B*52:01:01:17,
45:01~53/55~63/65~110/112~127 B*52:101, B*53:01:01:11,
B*53:01:26, B*53:63,
B*58:01:01:10, B*58:01:01:11,
B*58:01:36, B*58:01:37,
B*58:127

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 4 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

B*07:05:01:01~06:05/32/34/40/53/69/
78/80/90/97/105/112/123/137~138/
140/176/182N/201N/206~207/210/213~
214/222/258/262/264/269~270/278/
283/287/293/304/317/324/327/333/
340/352~353/356~358/368/378/387/
389/395/399; B*08:20:01~20:02/79;
B*13:16/20/31/48/62/128/131/145;
B*14:13/53/96; B*15:09:01~09:02/
30:01:01~30:01:03/42/58/73:01:01~
73:01:02/150/229/243/252/303/324/
329/361/435/453/460/486/541;
B*27:20/24/153/168; B*35:44/384/413;
B*38:10/32; B*39:18/35~36/107;
B*40:05:01:01~05:01:03/15~16:01:02/
23/26/28/51/95/148/158/161/174/183/ Changes:
198/309/355/466; B*42:19; B*44:459; B*51:104=B*51:104:01,
B*51:232=B*51:232:01
B*46:61/74; B*48:05/15;
New: B*13:145, B*14:96,
1 B*51:01:01:01~01:30/01:32~01:46/
B*40:466, B*51:01:01:65,
01:48~01:49/01:51~02:02/02:04~
3 04:02/06:01~07:02/11N~14/16/18/21~
B*51:01:01:66, B*51:01:01:67,
B*51:01:01:68, B*51:01:01:69,
5 30/32~35/38~39/41N/43/46~53/55~71/ B*51:01:01:70, B*51:01:85,
1 D 113H(N/K)114 152E---(-/L)156 150 - 94 19 0 0 75 1
B*51:01:86, B*51:01:87,
7 74~80/82~84/86/88~89/94~96/98N~107/
3 109~114/116~121/123~134/136~138/ B*51:02:01:03, B*51:104:02,
9 140/142~145/147/149N~152/154~156/ B*51:232:02, B*51:322,
5 B*51:324, B*51:325N, B*51:326,
11 158:01~171/173Q~180/182~193/195~
B*51:327, B*51:328, B*51:329Q,
7 196/198~205/208~210/212~215/218~ B*51:330, B*51:331, B*51:332,
9 219/221~222/224~226/228~253/255~ B*52:01:01:17, B*52:101,
258/260/262/264N~273N/277~279/282~ B*55:111, B*59:12
11 297/299~322/324~332; B*52:01:01:01~
01:25/01:27~02:02/04~09:02/11/13~
18/20~25:02/27~43/45~56/58~60/62/
64~68/70/72~73/76~87/89N~101;
B*54:20/33; B*55:01:01:01~01:26/03/
05/09/11/15/17/21/24~25/28:01~29/
31/33/36/38/40/44~45/52~55N/59~60/
64/66/73~76/78~79/84~85/87/90~95/
97N~106/108~109/111; B*56:05:01~06/
21/25/37/66; B*57:57/63; B*58:08:01~
08:02; B*59:12; B*78:01:01:02~03/05~
07/09; C*01:90/116; C*02:68; C*03:317;
C*05:238; C*12:282; C*14:92; C*15:24/
128

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 5 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

B*08:118; B*13:15/62; B*14:13/53/96;

B*15:435; B*18:26/61/97/191;
B*27:153; B*35:81/157/233; B*39:32/
48; B*40:28/51/113/163/174; B*44:459;
B*51:01:01:01~01:30/01:32~01:49/
01:51~01:87/03~04:02/06:01~08:03/
09:01~09:03/11N~14/16/18~22/24:01~
33/35/38~39/41N/43~44N/46~53/55~58/
60~80/82~84/86~89/91/94~107/109~
Changes:
134/136~142/145/147/149N~156/
B*51:104=B*51:104:01,
158:01~171/173Q~174:02/176~179/181~ 113H(N/K)114 171H---174 200 B*51:232=B*51:232:01
196/198/200~205/208~215/217~219/ New: B*14:96, B*51:01:01:65,
221~222/224~226/228~253/255~260/ B*51:01:01:66, B*51:01:01:67,
262~273N/275~280/282~294/296~297/ B*51:01:01:68, B*51:01:01:69,
299/301~322/324~332; B*52:01:01:01~ B*51:01:01:70, B*51:01:85,
C 02:02/04~10:03/13~25:02/27~56/58~ - 96 13 0 0 83 1 B*51:01:86, B*51:01:87,
B*51:08:01:03, B*51:08:01:04,
62/64~66/68~74/76~87/89N~101;
B*51:104:02, B*51:232:02,
B*56:05:01~06/21/34; B*58:08:01~ B*51:322, B*51:324, B*51:325N,
08:02; B*67:06; B*78:01:01:02~03/05~ B*51:326, B*51:327, B*51:328,
10 B*51:329Q, B*51:330, B*51:331,
B*51:332, B*52:01:01:17,
B*52:101
B*07:358; B*15:30:01:01~30:01:03/58/
1
73:01:01~73:01:02/150/303/324/453/
3 486/541; B*35:18/44/413; B*39:33;
5 B*40:324; B*41:62; B*46:61/74;
[156(W/-)156 + 162-
B*51:05/29/54/61:01~61:02/217/279; 113HN114 175
7
1 B*52:21:01~21:02/24/97; B*54:33/40;
L163]

9
3 B*56:43/51/63; B*57:02:01:01~02:02/
12/28N/42/63
11
5
7
A*24:309; A*32:39; B*15:16:01:01~
9 17:07/24:01~24:02/67/87/157/162/
11 168/177/196/208/216/222/230/254/
268/273/356/361~362/396/403/408/
411/423~424/442/446/500/516/523/
532/546Q/550/555/575N;
B*27:02:01:01~02:04/02:06/30/53/57/ New: B*15:17:01:04, B*15:575N,
62/65N/75/77/83/95/102/119/126/134/ B*27:02:01:06, B*27:02:06,
163/171~172/176N/181/188/197/203/ B*27:236, B*49:01:01:12,
B 62R-63 80I80 75 - 94 11 0 0 83 1
B*49:01:17, B*49:01:18,
213/232/236; B*37:34; B*38:25;
B*40:13/19/109/117/292/340/394; B*49:69, B*52:01:01:17,
B*52:101
B*41:46/60; B*44:18/25/50:01~50:03/
95; B*48:18; B*49:01:01:01~01:18/03~
25/27~62/64~66/68~69; B*51:231/310;
B*52:01:01:01~01:28/01:30~19/21:01~
72/74~101; B*53:17:01~17:02/28;
B*57:128; B*58:100

B*07:400; B*13:58; B*15:202/239/376;

B*18:12:01~12:02/195; B*27:52/236;
B*35:10/13/16/28:01:01~28:01:03/69/
80/226/270/318/477; B*37:01:01:01~ New: B*27:236, B*37:01:01:12,
B*37:01:01:13, B*37:01:01:14,
05/07/09~27/29~60/62~66/68~81/83~
B*37:01:23, B*37:01:24,
A 95; B*40:185/209/308/350/365; 45T45 63--Q65 100 - 96 6 0 0 90 1
B*37:01:25, B*37:01:26,
B*44:83/134/445; B*49:03; B*51:310; B*37:95, B*52:01:01:17,
B*52:01:01:01~01:41/01:43~01:48/03~ B*52:101
15/17/19~31:02/33~69/71~72/74~101;
B*53:17:01~17:02/28/38; B*78:05~06

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 6 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

Changes: B*35:51=B*35:51:01,
B*35:205=B*35:205:01,
B*07:65/134/400; B*08:32/133/180/ B*35:215=B*35:215:01,
239; B*15:202/239/308/376; B*35:460=B*35:460Q
B*18:01:01:01~03:01:03/04:01~08/10~ New: B*18:01:01:52,
36/38~47/50~53/55~65/68~78/80~106Q/ B*18:01:01:53, B*18:01:01:54,
109~124/126~131:01:02/133~ B*18:01:33:03, B*18:01:42,
B*18:01:43, B*18:01:44,
135:01:02/137~141/143~177/179~193/
B*18:03:01:03, B*18:07:03,
2 196~197/199~201; B*35:01:01:01~
B*18:196, B*18:197, B*18:199,
03:02/03:04~08:01:10/08:03~08:04/
4 08:06~18/20:01~24:02/28:01:01~45/
B*18:200, B*18:201,
B*35:01:01:51, B*35:01:01:52,
6 48/50~55/57~62/64:01~153/155~184/ B*35:01:01:53, B*35:01:01:54,
H 45T45 77S-RN-81 125 - 96 13 0 0 83 1
B*35:01:01:55, B*35:01:01:56,
8 186~197/199~202/204~206/208:01~209/
211~216N/218~221/223~253/255~270/ B*35:01:01:57, B*35:02:01:06,
10 272~273/275~298/300~310/312~316/ B*35:03:32, B*35:05:05,
B*35:08:01:09, B*35:08:01:10,
12 318~328/330~354/356~360/362~449/
B*35:08:14, B*35:29:03,
451~470/472~507; B*37:11/14/37/67; B*35:51:02, B*35:205:02,
B*39:19:01~19:02; B*40:185/209/308/ B*35:215:02, B*35:490,
350/365; B*45:22; B*46:40:01~40:02; B*35:491, B*35:492, B*35:493,
B*56:06/45; B*58:64; B*78:01:01:02~ B*35:494, B*35:495, B*35:496,
05/07~08 B*35:497, B*35:498, B*35:499,
B*35:500, B*35:501, B*35:502,
1 B*35:503, B*35:504, B*35:505,
B*35:506, B*35:507
3
5
7
9
11

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 7 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

Changes:
B*18:194=B*18:194:01:01,
B*27:215N(x),
B*44:23N=B*44:23:01:01N,
B*51:104=B*51:104:01,
B*51:232=B*51:232:01
New: B*13:02:01:15,
B*13:02:01:16, B*13:144,
B*13:145, B*13:146, B*13:147,
B*13:148, B*13:149, B*13:150,
B*13:151, B*14:93, B*15:13:03,
B*15:17:01:04, B*15:575N,
B*18:194:01:02, B*27:02:01:06,
B*27:02:06, B*27:05:02:22,
B*27:05:49, B*27:05:50,
B*27:05:51, B*27:05:52,
B*27:05:53, B*27:233, B*27:234,
B*27:235, B*27:236, B*27:237,
Bw4 (w/o B*13:28; B*13:70; B*15:403; B*27:238, B*37:01:01:12,
B*15:481; B*27:05:25; B*27:05:37; B*37:01:01:13, B*37:01:01:14,
B*27:49; B*27:116; B*27:122; B*27:156; B*37:01:23, B*37:01:24,
B*27:169; B*27:178; B*27:221; B*37:24; B*37:01:25, B*37:01:26,
1 B*37:58; B*37:59; B*37:64; B*37:90; B*37:95, B*38:01:01:12,
B*38:01:01:13, B*38:01:18,
3 B*38:60; B*38:61; B*38:76; B*38:169;
B*38:170, B*38:171,
B*44:03:16; B*44:03:30; B*44:111;
5
2 B*44:127; B*44:145; B*44:172;
B*44:02:01:44, B*44:02:01:45,
B*44:02:01:46, B*44:02:01:47,
7
4 B*44:200; B*44:255; B*44:458; B*44:02:01:48, B*44:02:01:49,
B*44:495; B*44:504; B*49:01:18;
9
6
B*44:02:67, B*44:02:68,
B*49:21; B*51:01:14; B*51:12; B*51:67; B*44:02:69, B*44:02:70,
11 G 43P43 82LR83 150 - 95 49 0 0 46 1
B*44:03:01:36, B*44:03:01:37,
8 B*51:100; B*51:159; B*51:171;
B*51:202; B*51:205; B*51:239; B*44:03:01:38, B*44:03:02:03,
10 B*51:266; B*51:291; B*51:314; B*44:23:01:02N, B*44:493,
B*44:494, B*44:496, B*44:497,
12 B*51:315; B*52:01:19; B*52:01:42;
B*44:498, B*44:499, B*44:500,
B*52:04; B*52:34; B*52:35; B*53:01:22; B*44:501, B*44:502, B*44:503,
B*53:10; B*53:52; B*57:01:08; B*49:01:01:12, B*49:01:17,
B*57:01:29; B*57:08; B*57:34; B*49:69, B*49:70,
B*57:105; B*57:114; B*57:117; B*51:01:01:65, B*51:01:01:66,
B*57:126; B*58:01:34; B*58:42; B*51:01:01:67, B*51:01:01:68,
B*58:54; B*58:70; B*58:81; B*58:125) B*51:01:01:69, B*51:01:01:70,
B*51:01:85, B*51:01:86,
B*51:01:87, B*51:02:01:03,
B*51:08:01:03, B*51:08:01:04,
B*51:104:02, B*51:232:02,
B*51:322, B*51:323, B*51:324,
B*51:325N, B*51:326, B*51:327,
B*51:328, B*51:329Q, B*51:330,
B*51:331, B*51:332,
B*52:01:01:17, B*52:101,
B*53:01:01:11, B*53:01:26,
B*53:63, B*57:01:01:17,
B*57:01:01:18, B*57:01:01:19,
B*57:01:01:20, B*57:01:44,
B*57:135, B*57:136, B*57:137,
B*57:138, B*57:139N, B*57:140,
B*57:141, B*58:01:01:10,
B*58:01:01:11, B*58:01:36,
B*58:01:37, B*58:127, B*59:11,
B*59:12

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 8 OF 9

One Lambda, Inc. 22801 Roscoe Blvd., West Hills, CA 91304 Tel: (747) 494-1000 Fax: (747) 494-1001 www.onelambda.com
 Approx. Number of

No Prev.
Revision Information

No. Test

R Value
Tray
Alleles Specificity 5' Recognition Site 3' Recognition Site Size

Lot
Specificity Changes: Added(+),
Pos.

FN

TN
TP

FP
(bp) Removed(-), Deleted Seq(x) or New

Bw6 (w/o B*07:02:67; B*07:13;

B*07:97; B*07:110; B*07:179; B*07:192;
B*07:195; B*07:244; B*07:257;
B*07:412; B*08:29; B*08:36; B*08:97;
B*08:101; B*08:114; B*08:131;
B*08:136; B*08:139; B*14:01:05;
B*14:02:05; B*14:02:08; B*14:30;
B*14:34; B*15:01:25; B*15:01:27;
B*15:01:49; B*15:277; B*15:311;
B*15:354; B*15:430; B*15:434;
B*15:521; B*18:01:21; B*18:01:24;
B*18:45; B*18:99; B*18:133; B*18:162;
B*18:179; B*35:01:39; B*35:01:52;
B*35:02:03; B*35:02:14; B*35:17:02;
B*35:152; B*35:222; B*35:248;
B*35:293; B*35:501; B*39:01:15;
B*39:24:02; B*39:100; B*39:104;
B*39:105; B*39:115; B*40:01:31;
B*40:02:22; B*40:02:29; B*40:76;
B*40:127; B*40:135; B*40:193;
2 B*40:212; B*40:217; B*40:218;
1 B*40:223; B*40:281; B*40:306;
4
3 B*40:311; B*40:327; B*40:335;
6 B*40:400; B*40:421Q; B*40:423; (See Notes section for Revision
5 F
B*40:436; B*41:02:02; B*41:05;
52-52 79RN--82 100 - 96 78 0 0 18 1
Information for this well.)
8
7 B*41:26; B*41:55; B*42:22; B*45:01:05;
10 B*45:01:09; B*45:16; B*45:21; B*46:48;
9
12 B*46:75; B*46:77; B*46:86; B*48:20;
11 B*48:36; B*50:38; B*50:46; B*50:53;
B*54:01:01:01; B*54:01:01:02;
B*54:01:01:03; B*54:01:01:04;
B*54:01:01:05; B*54:01:02;
B*54:01:03; B*54:01:04; B*54:01:05;
B*54:01:06; B*54:01:07; B*54:01:08;
B*54:01:09; B*54:02; B*54:03; B*54:04;
B*54:06; B*54:07; B*54:09; B*54:10;
B*54:11; B*54:13; B*54:14; B*54:15;
B*54:16; B*54:17; B*54:18; B*54:19;
B*54:20; B*54:21; B*54:22; B*54:23;
B*54:24; B*54:25; B*54:26; B*54:27;
B*54:28; B*54:29; B*54:30; B*54:31;
B*54:32; B*54:33; B*54:34; B*54:35;
B*54:36; B*54:37; B*54:38; B*54:39;
B*54:40; B*54:41; B*54:42; B*54:43;
B*55:01:13; B*55:73; B*55:88;
B*67:02:01:01; B*67:02:01:02;
B*81:06)

TDX-OLI-DMR-LOT-2101-1075 REV 01: PAGE 9 OF 9

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