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Cell Membrane Potential
Cell Membrane Potential
RT " [IonOut ] %
E Ion = ln$ '
zF # [Ion In ] &
Electrical Chemical
RT " [KOut ] %
EK = ln$ '
zF # [K In ] &
# [K ] & # [K ] &
EK !
= 25 " ln% Out ( E K = 58 " log% Out (
$ [K In ] ' $ [K In ] '
! !
# [K ] &
E K = 58 " log% Out (
$ [K In ] '
# [3mM] &
E K = 58mV " log% (
$ [90mM] '
!
E K = "85.7mV
!
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Experimental support for membrane hypothesis
Predicted Vm
Measured Vm
Why is measured Vm different than
predicted Vm?
Hodgkin and Keynes 1954
For permeant anions (z<0) like Cl(-), invert out and in:
gK E K + gNa E Na
Vm =
gK + gNa
At rest the cell is at a steady state and there is no
net current, however individual ions are still in flux.
!
Why doesn’t concentration gradient eventually run out?
With ATPase
(real cell)
Key Concepts
1. Resting membrane potential is determined
by selective membrane permeability to
specific ions at rest.