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Caffeine
Caffeine
Caffeine
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Research Article
China the primary choice is green tea. using suitable organic solvents, considering
However, the natural substances are not the protonation like acids [14].
invariably useful when consumption goes Materials and Methods
beyond an average standard [5]. Recent Sampling
research has revealed lots of advantages with Total six different green and black tea leave
few side effects of tea [6]. Black and green samples were purchased from local market of
tea furnish a valuable source as it aids to Quetta city. The samples collected contained
sustain no accumulation of cholesterol, four branded (two green and two black) and
comfort the problem of vomiting, makes two open tea leaves (one green and one
activation of mental condition, helps in black). Samples of black tea leaves like open,
somehow to cure cancer [7]. tapal and lipton were named as sample A,
Both types of tea are boozing in all segments sample B and sample C respectively while
and domains of all age groups. Hence, green leaves like open,tapal, and lipton were
caffeine concentration in tea has indisputable named as sample a, sample b and sample c
crucial role in the homeostasis of a person. respectively.
On the basis of body per Kg weight only 0.3 Washing of glass ware
mg of caffeine is allowed [8]. In United The chromic acid solution was used for
States of America, caffeine absorption via soaking of all the beakers, and flasks,
different supplements is about 80-85 percent. deionioized water was used to clean them.
Caffeine as a drug in tea emanates from Extraction
alkaloid and reveal defense towards pests [9]. Chloroform was used as a main solvent for
Drinking of black tea extensively, influence extraction. To conduct the test 10 gram (g) of
the mind and physiological change in tea, 100 mL of distilled water and 10 g of
attitude. The over oxidation of leaves result calcium carbonate (CaCO3) were added in a
in more caffeine formation, and high uptake 250 mL conical flask. The mixture was
induce addiction. Caffeine proves to be boiled at 100 °C with constant stirring for 20
hazardous in pregnancy condition, in a day minutes. Than this mixture was filtered and
only 0.2 mg is believed to be secure [10]. filtrate was poured into another clean 250 mL
When high amount of tea is consumed, flask. With gradual shaking, 70 mL
negative impacts as shrinkage of blood chloroform was added into the filtrate.
vessels, nervousness arouse, imbalance of Mixture was shaken for 4 to 5 minutes and
urination and more importantly prompt the then left to stand for further 5 minutes, the
chances of heart failure [11]. Lots of surveys aqueous and organic layers appeared and
take notice of caffeine antioxidant peculiarity separated. The lower layer of chloroform
but recent literatures necessitate that quantity contained soluble caffeine which was
facts are focused in regard to fitness [12]. collected in small beakers while the upper
FTIR (Fourier Transmittance of Infra Red) is water layer was discarded. Further, small
an effective and advance analytical technique beakers were covered with petri dish and kept
used for element quality in a substance, and for heating on sand bath for 2 hours at 40 °C.
to ascertain the quantity, a specific and error The chloroform was evaporated to obtain
free UV-spectrophotometer is reliable [13]. caffeine.
However, caffeine should be extracted by
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Pure Appl. Bio., 4(1):9-14, March- 2015
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Samiullah et al.
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Pure Appl. Bio., 4(1):9-14, March- 2015
limits.The results show that the samples A,B, information should be given about the drug to
and C contain 114, 110 and 118.5 ppm, while aware the sport person, hence monitoring of
samples a, b and c possessed the its manufacturing, oxidation and packing
concentration 112, 28 and 114.5 ppm, which must center.
shows that the sample C and c of black and References
green tea contain higher amounts of caffeine 1. Rabiul-Islam GM, Gias-uddin M,
respectively. Similar findings were reported Mahfuz-ur-Rahman M&Yousaf A
by many researchers [2, 6-8]. (2013/). Caffeine and
The comparison of amount percentage of totalpolyphenols content of market
black and green tea leaves reveals that black tea cultivated and processed in
tea contains higher concentrations of Bangladesh. Mal. J. Nutr., 19(1):143-
caffeine. The amount of caffeine in sample C 147.
is about 21 percent higher and other samples 2. Luo M & Peng H(2012). Study on
contain in the series of sample c > A > B > a determination of content of the main
> b which are 20, 19, 17, 17 and 6 % components of tea polyphenlos. Inter.
respectively. Similar findings were reported J.of biosci.Biochem.and bioinform.
by many researchers [3, 11-14]. 2(6):433-437.
Conclusion 3. Ali MM, Eisa M, Taha MI, Zakaria
The UV-spectroscopy technique was BA &Elbashir AA (2012).
skillfully applied here for the evaluation of Determination of caffeine in some
vital amount of caffeine in complex Sudanese beverages by High
compounds of sample like tea which was PerformanceLiquid Chromatography.
procured from the shop of Quetta, Pakistan. Pak J of Nutrition, 11(4), 336-342.
The information gained directly from FTIR 4. Atomssa T &Gholap AV (2011).
showed the inspected caffeine presence and Characterization of caffeine and
the data was helpful to know the quantity of determination of caffeine in tea leaves
caffeine present in different kinds of tea using UV visible spectrometer. Afri.J.
brands. The result of our study is to assess of pure and appl. Chem., 5:1-8.
and compare the caffeine quantity in black 5. Vuong VQ, Nguyen V,Golding BJ&
and green tea and to appraise that the black Roach PD (2011). The content of
tea contains higher quantity of caffeine as bioactive constituents as a
compared to green tea. qualityindex for Vietnamese tea.
On the basis of relative findings of Inter. Food Res. J., 18: 329-336.
this research it can be set forth for the future 6. Sumary DP, Joesph WMP, Jomanga
recommendation to investigate the factors KE&GomezuluE (2011) Indicative
which varies the caffeine limit in alike plants investigation of amount caffeine in
of unlike areas and further how quantitative some commercial teas and coffees
reaction of caffeine differs from person to foundin Dodoma Market. Tanz. J. of
person, caffeine that matters with age and of nat. and appl. Sci.,2(1):323-327.
working class of varying regions despite of 7. Maughan JR&Griffint J (2003).
the strong and weak tea. Also, work on Caffeine ingestion and fluid balance.
contribution of those drinks containing J.Hum.Nutr. Dietet.16: 411-420.
caffeine that probably not put forward the 8. Zuo Y,Chen H& Deng Y (2002).
normal cup of 50 mg, beside of the mentioned Simultaneous determination of
amount referred for physical practice of catechins, caffeine and Gallic acid in
body. Moreover at national level the green, oolong, black and pu-erh teas
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Samiullah et al.
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