Projeto Genotropic Keywords.

Genotyping, Exotic plants,

Autochthonous plants, Resistance
F. Soares, I. Lamounier, I. Dias, M. Barbosa

Dr. Manuel Curto. Porto, Maia, Portugal

Acknowledgements
al19603@aemaia.com We would like to thank professor Luísa
Santos.

Abstract. This project aims to respond to the

rapid spread of fires, encouraging the Also to Dr. Manuel Curto for helping us in
exchange of exotic plants for indigenous plants, this project of ours and for its availability.
in places where there are plants less resistant
References
to fires and other environmental factors, namely
extreme temperatures. [1] G. Matthijs et al., “Guidelines for diagnostic
next-generation sequencing”, European Journal
Knowledge of this area has increased due to of Human Genetics, vol. 24, no. 1, pp. 2–5, Oct.
the progress of investigations, with the help of 2015,
genotyping methodologies, which are essential
tools in DNA analysis to identify genetic [2] E. Samorodnitsky et al., “Evaluation of
variations. There are several methodologies hybridization capture versus Amplicon‐Based
methods for Whole‐Exome sequencing”,
used for this purpose, however, its high cost
Human Mutation, vol. 36, no. 9, pp. 903–914,
turns out to be one of the limitations. One of the Jul. 2015
most common techniques is PCR (Polymerase
Chain Reaction) that amplifies specific regions [3]
of DNA. On the other hand, NGS (Next https://nebula.org/blog/pt-br/metodos-de-tes
Generation Sequencing) allows the analysis of te-de-introducao-ao-dna/
millions of DNA fragments simultaneously.
[4]
In this way, our project intends to investigate https://www.3tres3.com.pt/artigos/pcr-como
and announce new information about a topic -ferramenta-de-diagnostico-2-2-usos-e-inte
already studied superficially - the level of rpretacão_15386/
resistance of the plants. This will only be
possible through the use of the genotyping
technique, where the procedures may vary
depending on the technique used, but usually
involve common steps, such as material
extractions from the different plants, after that,
fragment the DNA and amplify specific regions
of the genome by means of PCR to be then
sequenced.

The project in which we are involved seeks to

understand the genetic diversity within plant
populations. Thus, by investigating the plants
most resistant to the factors mentioned, we can
not only contribute to the preservation of
natural ecosystems, but also develop more
effective forest restoration and management
strategies.