Biology : the essentials 3rd Edition

Mariëlle Hoefnagels
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THIRD E DIT I ON

BIOLOGY THE ESSENTIALS

Mariëlle Hoefnagels
T H E U N I V E R S I T Y O F
O K L A H O M A

MEDIA CONTRIBUTIONS BY

Matthew S. Taylor
BIOLOGY: THE ESSENTIALS, THIRD EDITION

Published by McGraw-Hill Education, 2 Penn Plaza, New York, NY 10121. Copyright © 2019 by McGraw-Hill
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Library of Congress Cataloging-in-Publication Data

Hoefnagels, Mariëlle, author.

Biology: the essentials / Mariëlle Hoefnagels, The University of
Oklahoma; media contributions by Matthew S. Taylor.
Third edition. | New York, NY: McGraw-Hill Education, 2018.
LCCN 2017034401 | ISBN 9781259824913 (alk. paper)
LCSH: Biology—Study and teaching (Higher)
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Brief Contents
UNIT 1 Science, Chemistry, and Cells
1 The Scientific Study of Life 2
2 The Chemistry of Life 20
3 Cells 48
4 The Energy of Life 68
5 Photosynthesis 84
6 Respiration and Fermentation 98
UNIT 2 DNA, Inheritance, and
Biotechnology
7 DNA Structure and Gene Function 112
8 DNA Replication, Binary Fission, and
Mitosis 138
9 Sexual Reproduction and Meiosis 154
10 Patterns of Inheritance 170
11 DNA Technology 196
UNIT 3 Evolution and Diversity
12 Forces of Evolutionary Change 216
13 Evidence of Evolution 238
14 Speciation and Extinction 254
15 Evolution and Diversity of Microbial Life 272
16 Evolution and Diversity of Plants 300
17 Evolution and Diversity of Animals 318
©Shutterstock/Yavuz Sariyildiz
UNIT 4 Ecology
18 Populations 356
19 Communities and Ecosystems 372
20 Preserving Biodiversity 402
UNIT 5 Plant Anatomy and Physiology
21 Plant Form and Function 420
22 Reproduction and Development
of Flowering Plants 442
UNIT 6 Animal Anatomy and Physiology
23 Animal Tissues and Organ Systems 460
24 The Nervous System and the Senses 476
25 The Endocrine System 502
26 The Skeletal and Muscular Systems 516
27 The Circulatory and Respiratory
Systems 534
28 The Digestive and Urinary Systems 556
29 The Immune System 580
30 Animal Reproduction and Development 598
iii
About the Author
Mariëlle Hoefnagels is a professor in the Department of Biology and the Department
of Microbiology and Plant Biology at the University of Oklahoma, where she teaches courses in

introductory biology, mycology, and science writing. She has received the University of Oklahoma

General Education Teaching Award and the Longmire Prize (the Teaching Scholars Award from

the College of Arts and Sciences). She has also been awarded honorary memberships in several

student honor societies.

Dr. Hoefnagels received her BS in environmental science from the University of California

at Riverside, her MS in soil science from North Carolina State University, and her PhD in plant

pathology from Oregon State University. Her dissertation work focused on the use of bacterial

biological control agents to reduce the spread of fungal pathogens on seeds. In addition to authoring

Biology: The Essentials and Biology: Concepts and Investigations, her recent publications have

focused on creating investigative teaching laboratories and integrating technology into introductory

biology classes. She also maintains a blog on teaching nonmajors biology, and she frequently gives
©Davenport Photos
presentations on study skills and related topics to student groups across campus.
DEDICATION
To my students
Mariëlle Hoefnagels
 An Introduction for Students Using This Textbook
I have been teaching nonmajors biology at the University of Oklahoma
since 1997 and over that time have encountered many students who fear
• Summary Illustrations Created specifically for the summary,
these figures tie together the material in a visual way to help you
science in general and biology in particular. The complexity, abstrac- learn relationships 466 UNIT 6 Animal Anatomy and Physiology

tions, and unfamiliar terms can be overwhelming, and some students among the topics in
believe they can’t do well because they’re just not “into science.” In the chapter. See if Burning Question 23.1 23.3 Organ Sy
writing this book, I have focused on students and what you need to be you can explain the The tissues described in se
successful in a nonmajors biology class. relationships in your How does the body react to food poisoning?
This section provides a b
In a healthy body, the organ systems operate utes to the body’s function
In my experience, a big part of the problem is that many
Chapter 23 Animal Tissues and Organ Systems 473
students own words, then go so seamlessly that you are unlikely to no- function of each one relies
tice them. But suppose you eat food that is
just don’t have the right study skills—they focus too much on superficial back to review any tainted with bacteria, viruses, mold, or other
Question 23.1).

learning such as memorizing definitions, but they don’t immediately grasp sections you have contaminants. Within a few hours to a few
days, your body’s reactions are hard to miss. A. The Nervous an
the power of C. The Digestive, Circulatory, and Respiratory Systems Work Together
to Acquire Energy the material. I’ve created the following fea-
understanding trouble explaining. First, receptors in the digestive system

• signal the brain that toxins are in the gut.

Coordinate Comm
Bodies tures to help you
• Themake the
digestive transition
system fromThe
provides nutrients. memorizing to obtains
respiratory system understanding. Progress Bars The The brain responds by triggering vomiting, The human nervous system

•
O2, and the circulatory system delivers nutrients and O2 to tissues. which ejects partially digested food from ©Alix Minde/PhotoAlto/ specializes in rapid comm
es of the structure and bars found at the the stomach. Meanwhile, water moves from Getty Images RF detect stimuli; others relay
Concept•MapsThe body’s Acells
new use O to extractthe
Survey2
energy concept map at the
from food molecules.
Landscape
The circulatory and respiratory systems eliminate the waste CO . bottom of most pages the circulatory system into the intestines. This fluid contributes to other neurons carry impuls
start of each
ues. Tissues build organs,
D. The chapter illustrates Immune,
Urinary, Integumentary, how the andpieces
Lymphatic ofSystems
2
the entire unit fit diarrhea that flushes toxins out of the body. which contract or secrete p
should help you keep Vomiting and diarrhea dehydrate the body. In response, endo- The endocrine system
together. Each
Protectchapter
the Body ends with a Pull It Together concept map crine glands release a hormone called ADH (antidiuretic hormone) communication molecules
• The urinary system removes metabolic wastes from the blood and in mind where you are into the blood. ADH travels in blood vessels and binds to receptors in
metabolism, and many oth
e Types that makes connections
reabsorbs between key terms within the chapter.
useful substances. the kidneys. The kidney’s cells respond by saving water, returning it
in the chapter’s big to the blood instead of eliminating it in urine.
system and stimulate a ch
cellular matrix of water,
• Theconcept
Using these integumentary maps systemtogether will help
provides a physical barrieryou
betweenunderstand
the body how Some forms of food poisoning are accompanied by fever, shivers, relatively slowly, but their
rs. picture.
•
and its surroundings. and fatigue. These are responses of the immune system as it fights
the major• topics covered throughout the book relate
cancer.to one another.

•
The immune system protects against infection, injury, and
Why We Care These invaders. A feverish body is inhospitable to some bacteria. The shivers—
B. The Skeletal an
o forms glands. This tissue
Learn How • Theto Learn
lymphatic Each
system chapter
connects in this
the circulatory booksystems,
and immune contains a tip a rapid series of muscle contractions—help raise the body’s tempera-
and Move the Bod
nd secretes substances. passing the body’s fluids through the lymph nodes. boxes reinforce the ture. And what about fatigue? The body uses a lot of energy to maintain
that focuses
ied (more than one layer), E. TheonReproductive
study skills Systemthat build
Produces understanding.
the Next Generation Don’t try to a fever and to produce immune cells. Ordinarily, we eat to replenish our The skeletal system consis
mnar. applications of specific reserves. But if food and liquids won’t stay down, the digestive system protect underlying soft tiss
implement • them
The all
male and at
femaleonce; choose
reproductive one
systems are that appeals
essential to you and
for the production cannot absorb nutrients or water. Both nutrient depletion and dehydra- marrow within some bones
es Together of offspring. topics to the real tion contribute to low energy.
add more• as you determine what works best for you. minerals such as calcium.

•
functions. Most consist Figure 23.12 summarizes the human body’s organ systems. world. Individual skeletal m

•
Submit your burning question to
matrix.
What’s the Point? This brief introduction helps explain the marielle.hoefnagels@mheducation.com system. When a skeletal m
oose connective tissue, dense 23.4 Organ System Interactions Promote Homeostasis Burning Question In support a person’s posture
e, and blood. importanceHomeostasis
of the chapter topic. A companion feature is What’s
• is stability in the internal environment. Animals may maintain this feature, I answer (fire):
Figure ©Ingram
23.6 Human Publishing/SuperStock RF
tracting skeletal muscles a
Applied,in which
the Point? homeostasis appears
body temperature and innear the end
the chemical of each
composition of the chapter
Organ Systems.
hen protein filaments slide blood plasma and the interstitial fluid. questions from Communication Support and move
and builds• Inonnegative
the chapter’s content by explaining a wide-ranging
feedback, sensors detect a change in the internal environment, students who are either in my classes Nervous systemor who have written
Endocrine system to me Skeletal system
ac, and smooth muscle.topic that is
andrelevant to activates
a control center your life.effectors that counteract the change. The
with a “burning question” of their own.
•
ation Network overall effect is to restore the parameter to its normal range.
e. • Positive feedback reinforces the effect of a change.
Miniglossaries Most chapters have one or more miniglossaries,
euroglia support neurons.
in an animal’s body. Nervous system controls the Food and drinks brief lists of key terms that help you define and distinguish between
skeletal and muscular systems,
which balance and move the body.
previously entered the
digestive system. interrelated ideas. You can use the miniglossaries to create flashcards,
ected
concept maps, and other study aids.
•
dinate Communication Male
ordinate all other organ Scientific Literacy These new thought questions at the end of
Respiratory system each chapter will help you practice thinking like a scientist about
ate rapidly, whereas absorbs O2 and
relevant social, political, or ethical issues. Female

•
ct more slowly. gives off CO2. Integumentary system
gives off excess heat Detects, interprets, and Produces hormones and Provides framework for S
rt and Move the Body produced by active Connect® The content in thisresponds textbook to stimuli is
outside and within the
fromintegrated with
works with the a wide muscles
nervous
system to control many
to attach, making
movement possible. Houses
e
H
upport the body. Bones also muscles.
Endocrine system
variety of digital tools available in Connect
body. With endocrine
system, coordinates all
that will help you
body functions, including
reproduction, response to
learn bone marrow. Protects soft
organs. Stores minerals.
te

ove and generates body heat. helps regulate

A Summary
Functions
Cover interior and
exterior surfaces of
organs; protection;
secretion; absorption
Support, adhesion,
insulation, attachment,
and transportation
Movement
Rapid communication
among cells
heart rate, metabolic
Circulatory system the connections and relationships that are critical to understanding
organ functions. stress, and metabolism.
transports O2, water,
rate, and body fluid
composition.
food molecules, how biology really works. Specialized Cells Build Animal Bodies Animals Consist of Four Tissue Types Organ Systems Are In
hormones, and
metabolic wastes.
Although developing study skills is a major step on the pathway to
Wastes accumulate in Lymphatic system success, a student’s mindset is important too. If you believe that you can
collects and
the digestive and
urinary systems for transports plasma develop your talents for biology—even if it takes some hard work—then
leaking out of
elimination later.
blood vessels. you set the stage for a successful semester. Anyone can be a “science
person.”
Immune system
Gametes develop in protects the body I hope that you enjoy this text and find that the study tips and tools
from infection if
reproductive system.
injury occurs. help you develop an understanding of biology.
Mariëlle Hoefnagels
Figure 23.12 Organ System Functions: A Summary.
Photo: ©James Woodson/Getty Images RF

vi
 Burning Question 4.1 Do hand sanitizers work?
Investigating Life 4.1 Does Natural Selection Maintain Cystic Fibr

olution and Diversity

Author’s Guide to Using This Textbook
s of Evolutionary Change This guide
Burning listsRiding
Calories. key chapter features
a bicycle takes and comes
energy, which describes
from some of the ways that I use them in my own classes.
metabolic reactions inside cells.
LEARNING OUTLINE
©maxpro/Shutterstock RF
12.1 Evolution Acts on Populations
12.2 Evolutionary Thought Has Evolved for Centuries
12.3 Natural Selection Molds Evolution
12.4 Evolution Is Inevitable in Real Populations
12.5 Natural Selection Can Shape Populations
in Many Ways Learn How to Learn
Learn How
S UtoRV E Y study
Learn T H E tips
L A help
N DS CA P E
12.6 Sexual Selection Directly Influences Reproductive studentsScience,
develop their study skills.and Cells
Chemistry,
Success
Focus on Understanding, Not Memorizing
12.7 Evolution Occurs in Several Additional Ways When you are learning the language of biology, be sure to concentrate on Each chapter has one Learn How to Learn
how each new term fits with the others. Are you studying multiple components is the study of Biology
APPLICATIONS
of a complex system? Different steps in a process? The levels of a hierarchy? studyLife
tip, and a complete list is in Appendix F.
Why We Care 12.1 Dogs Are Products of Artificial Selection
©Davenport
Burning Question Photos
12.1 Is there such a thing as a “pinnacle of evolution”? As you study, always make sure you understand how each part relates to the I present
consistsa Study
of units
carryMinute in class each week,
out the
whole. For example, you might jot down brief summaries in the margins of your
withcalled
examples of how
of to use these study tips.
Why We Care 12.2 The Unending War with Bacteria
Investigating Life 12.1 Bacterial Evolution Goes “Hog Wild” on the Farm functions
notes, or you could use lists of boldfaced terms in a chapter to make your own
concept map. ATP makes Respiration
Cells uses
pinefish swallows Chapter 12 Forces of Evolutionary Change 237 consist of Carbohydrates
r many predators to
Why do porcupinefish makes
provides the answer.

W R I TE I T O UT PULL IT TOG ETHER Molecules include Proteins Photosynthes

1. List and describe five mechanisms of evolution. does not
Evolution
occur at
Hardy– consist of encodes such as
S2.UHow
RV did EYtheTHEwork ofLAN D SCAP
other scientists E Charles
influence Weinberg
Darwin’s thinking? equilibrium
tes Evolution and Diversity is a change in catalyze the
3. Explain how understanding evolution is important to medicine, acts on a
ey consider “important” during a
Evolution agriculture,has
and maintaining
occurred the diversity of organisms
throughout on Earth.
Life’s history
a population’s Atoms DNA Enzymes cell’s chemical
grams. Both strategies risk losing
n ideas that could help in later
ring lecture, including examples,
4. Write a paragraph that describes the connections among
occurs by require
the following terms: gene, nucleotide, allele,by is deduced
phenotype, has a collection
Concept maps help students see the big picture.
reactions, including
studying Population Gene pool
e instructor uses. It will be much population, genetic variation, natural selection, and evolution. of genes and
te picture of what happened in Natural selection
5.
and other Jellyfish Lake,
Genetic
located
variation on the Pacific island of Palau, is home is a group of alleles called a
New Survey Allthe
cells require energy
Landscape in the
concept formatofthe
maps ATPstart
to carry out th
Fossils, DNA,
mechanisms to millions of jellyfish. Many years ago, sea and levels dropped
other
interbreeding
chemical reactions, acquire resources, and power their
may lead to
exists among
and the jellyfish were trapped
members
from the
in the basin. The
present and
evidence
lake houses of each chapter illustrate how the pieces of the entire
other activities. Enzymes are proteins that speed these
no predators, andof the jellyfish’s stinglefthas weakened.
provide Jellyfish
unit fit together. These new figures integrate with the
each past have Organisms
Lake is now a popular tourist attraction where snorkelers
Reproductive define Species
information
to build
barriers can swim among the jellyfish. Explain how Jellyfish Lake is
evolves by
reactions.
evidence for evolution.
Nonrandom Genetic Natural
existing Pull It Together concept maps in the chapter
For more details, study the Pull It Together feature in the chapter summ
can be causedPhylogenetic
6. Influenza
summary.
includes formation and smallpox are diseases bytrees
different Migration Mutations
and extinction of mating drift selection
types of viruses. Scientists arranged into
must produce a new influenza
vaccine each year, whereas the
Evolution occurs in many ways. The most familiar mecha-smallpox vaccine eradicated
each of which
nism is natural
generation to
the selection,
disease. Explain
perspective.
these results
but genetic changesfrom an
the next also happen by mutation, genetic
fromevolutionary
one has a unique
combination of
After spending class time discussing the key points in
7. Explain
drift, nonrandom how harmful
mating, recessive alleles can persist in
and migration.
populations, even though they prevent homozygous individuals
constructing concept maps, I have my students draw
mix randomly conferring adaptive
concept maps of their own.
For more details, study the Pull It Together feature in the chapter summary.
from reproducing.
unevenly change in are traits become
8. Fraggles are mythical, mouselike creatures that live underground move between in frequency in generated more common in
beneath a large vegetable garden. Of the 100 Fraggles in populations in by
this population, 84 have green fur and 16 have gray fur. A Alleles
dominant allele F confers green fur, and a recessive allele f
confers gray fur. Assuming Hardy–Weinberg equilibrium is operating, Figure 12.24 Pull It Together: Forces of Evolutionary Change.
answer the following questions. (a) What is the frequency of the gray
allele f ? (b) What is the frequency of the green allele F? (c) How many
Fraggles are heterozygotes (Ff )? (d) How many Fraggles are homozygous
recessive (ff )? (e) How many Fraggles are homozygous dominant (FF )? Refer to figure 12.24 and the chapter content to answer the following questions.

8
9. Describe the competing selective forces acting on peacock tails. Together, 1. Review the Survey the Landscape figure in the chapter introduction.
do these selective forces produce disruptive, directional, or stabilizing When has evolution occurred in life’s history? How do scientists know
selection? UNIT
that evolution has occurred in the past?2 DNA, Inheritance, and Biotechnology
10. Some researchers suggest that a giraffe’s long neck results from competition
for foliage with other types of animals; others say it is the product of sexual the concept map would occur. DNA Replication, Binary Fission, and Mitosis
2. Describe situations in which the five mechanisms of evolution shown in

selection. How might each mechanism explain how a long-necked species 3. Add the terms genotype, phenotype, allele frequencies, founder effect,
evolved from an ancestral population with short necks? How does each bottleneck effect, and sexual selection to this concept map.
explanation compare to how Lamarck might have explained it?
LEARNING OUTLINE
Cells Divide and Cells Die
DNA Replication Precedes Cell Division
TheS CLearning Outline introduces the
I EN TI F IC LITERACY Bacteria and Archaea Divide by Binary Fission

chapter’s
Burning Questionmain headings
12.1 explains andbe adapted
why an organism cannot helps to all
environments. Even humans cannot live in all parts of the world. However,
Replicated Chromosomes Condense as a Eukaryotic Cell
Prepares to Divide
students keep
technology allows us to livethe big
in habitats picture
that would in
otherwise be mind.
deadly.
example, the cold temperatures, low oxygen level, and lack of food on a
For Mitotic Division Generates Exact Cell Copies
Cancer Cells Divide Uncontrollably
high mountain would soon kill an unclothed man. However, the same man
Each heading is a complete sentence that
could survive indefinitely in the same location if he had a warm house and
clothing, breathed from an oxygen tank, and grew food in a sophisticated
APPLICATIONS

summarizes the most important idea of

greenhouse. Are human technologies the products of natural selection? Is
a human who lives in a harsh environment “adapted” to that environment,
Answers to Mastering Concepts, Write It Out, Scientific Literacy, and
Pull It Together questions can be found in the Connect ebook.
Burning Question 8.1 Do all human cells divide at the same rate?
Why We Care 8.1 Skin Cancer

the section. Students can also flip to the

even if he must use technology to survive? What areas of the world might be
especially difficult to inhabit, even with the use of current or potential future
connect.mheducation.com Investigating Life 8.1 Evolutionary Strategies in the Race Against Cancer

end of the chapter before starting to read;

technologies? Why? Design element: Burning Question (fire background): ©Ingram Publishing/Super Stock

the chapter summary and Pull It Together

concept map can serve as a review or
provide a preview of what’s to come. Growth. Cell division accounts for the growth of a seedling, a child,
and every other multicellular organism.
©PhotoAlto/Getty Images RF

Learn How to Learn S URV E Y T HE LA N DS CA P E

Write It Out—Really! DNA, Inheritance, and Biotechnology
Get out a pen and a piece of scratch paper, and answer the open-ended vii
“Write It Out” questions at the end of each chapter. This tip applies even if the is
DNA manipulated DNA technology
exams in your class are multiple choice. Putting pen to paper (as opposed to
in
just saying the answer in your head) forces you to organize your thoughts and undergoes encodes
undergoes
THINK you know.
Mutations Proteins carry out Replication
 234 UNIT 3 Evolution and Diversity

Investigating Life 12.1 | Bacterial Evolution Goes “Hog Wild” on the Farm
Investigating Life boxes focus on what
Although infectious diseases were once the leading cause of human death,
antibiotics had made many bacteria-caused diseases manageable by the mid- introductory science students need:
1900s. Since that time, bacteria have become resistant not only to the original
penicillin but also to the many manufactured antibiotics that followed it. Now an understanding of the process of science, an ability
antibiotic-resistant bacteria are common, creating new obstacles for physicians
treating infectious disease.
to interpret data, and an awareness of how scientific
Medical practices contribute to the rise of antibiotic-resistant bacteria, but
so do farms. Antibiotics promote rapid animal growth when added to the food
research contributes to our understanding of evolution.
of cattle, chickens, swine, and other livestock. This practice comes at a cost 100
to public health. The animals’ manure contains not only antibiotics but also Each box describes a real experiment focusing on an

Number of resistance
80
bacteria that are resistant to the drugs. These microbes swap genes with their

neighbors (see figure 8.7). Farms have therefore become breeding grounds for
antibiotic-resistant bacteria.
To learn more about this problem, researchers from China and the Unit-
ed States collected manure from three Chinese pig farms where antibiotics
are used. Control manure came from pigs that had never been exposed to
the drugs. When the team tallied the number of resistance genes in bacterial
DNA extracted from each sample, they found that manure from antibiotic-
treated animals had many more resistance genes than did control manure
(figure 12.A).
But farmers often compost pig manure and then spread it on their crop-
lands. Do the genes persist under those conditions? To find out, the researchers
collected samples from compost piles and from the soil in nearby fields. DNA
analysis revealed that compost and soil from farms using antibiotics had more
diverse resistance genes than did soil from a forest.
These results have serious implications, and not just for farm workers.
Since composted animal manure is spread over fields, crops may become con-
taminated with antibiotic-resistant bacteria. Meat from treated livestock may
also harbor resistance genes. When we eat the crops or the meat, bacteria in our
intestines may take up the resistance genes.
Source: Zhu, Yong-Guan, and seven coauthors, including James M. Tiedje. 2013. Diverse and abundant antibiotic resistance genes in Chinese swine farms. Proceedings of the National Academy of Sciences,
vol. 110, pages 3435–3440.
genes detected
60 evolutionary topic related to the chapter’s content. The
40 studies touch on concepts found in other units; you can
20 encourage students to draw a concept map illustrating
0
Control Farm 1 Farm 2 Farm 3
the relationships between ideas. You might also use the
Manure sampling site case as a basis for discussion of the nature of science.
Figure 12.A Antibiotic Resistance on the Farm. The diversity of antibiotic-
resistance genes was significantly higher in the manure of antibiotic-fed pigs
than in that of untreated animals. Error bars represent the standard error of the Assignable Connect activities contain questions
focused on the process of science, data interpretation,
mean (see appendix B).
Changes in government policy and consumer awareness may soon decrease
the use of antibiotics on farms. As demand for meat from antibiotic-free animals and how the study contributed to our understanding of
grows, farmers will have an economic incentive to find alternatives to the drugs.
The need for change is urgent because some antibiotics may become useless if evolution.
current practices continue. Evolution never stops, but a thorough understanding
of natural selection and bacteria can help us slow the rise of antibiotic resistance.

Chapter 5 Photosynthesis 89

What’s the Point? APPLIED

chlorophyll, in turn, replaces its “lost” electrons by splitting water molecules,
Evolution occurs in every population; bacteria are
What’s the Point? and What’s the Point? Applied boxesLight
yielding
evolutionOof as a waste product. help Hrelate
2 HIV, for example, has made this virus a
O 2 CO2

These twoan resources

effective (energy and “loaded”
has yet electron carriers) set the Chloroplast
no exception. The evolutionary changes in microbes
have important implications in human medicine.
chapter topics to life outside the classroom.
moving
stage
to befor
target;
the second
invented.
anti-HIV vaccine
partunderstanding
A better of photosynthesis:
of how thethe carbon reactions. In
What’s the Point?
Why We Care 12.2 and Investigating Life 12.1 high- the carbon
virus and evadesthe
evolvesreactions, chloroplast
the immune system uses ATP, the high-energy
might
light one problem: In the hundred years since the These boxes can be used as a starting point for traditional lecture or as the basis
lead to better
electrons treatments
in NADPH, andagainst
CO2 HIV.
to produce sugar molecules. These
discovery of antibiotics, many strains of resistant 4.1 All Cells Capture and Use Energy
Studying evolution has led to innovative
reactions are the “-synthesis” part of photosynthesis. The ATP and
ATP
bacteria have appeared. Health“I wish I had yourcan
professionals metabolism!” Perhaps for class discussion.
ideas in the treatment of disease. For example,
NADPH come from the light reactions, and the CO2 comes from
you have overheard
minimize this problem by prescribing antibiotics a calorie-counting You’re running late. You overslept,ofyou
the evolution have no time for breakfast,
chemotherapy-resistant cancer and you Light NADPH Carbon
the atmosphere (seeresearchers
Burning Question 5.2). Once inside the leaf,
only when needed and by friend educating
makethe public comment to
a similar have a full morning ofcells classes.
has You rummage
inspired through to your
develop cupboard
new and find reactions reactions
CO diffuses into a mesophyll cell and across8.1).through the mem-
the chloroplast NADP+
about the proper use of the drugs.
someone who stays slim on a diet of something called an drug 2 delivery
“energy strategieswhat
bar”—just (see Investigating
you need toLifeget Chapter 4 The Energy of Life 81
Viruses also evolve. The surprising and some- ©PhotoAlto sas/Alamy Stock Photo RF brane
Also, into the stroma,
studying where
intestinal worms thehas
carbon
led toreactions
new occur. ADP
fattening foods. morning. But what is energy?
times deadly appearance of new influenza strains such as H1N1 is evidence theories about the origin of Overall, photosynthesis
allergies. As is an oxidation–reduction
explained in Investigating Life 29.1, (redox) process.
In thatmutations
of how viruses change over time. Rapid context,inthe
viralword
DNA provide enor- these findings may make “Oxidation” means that electrons are removed from an atom or molecule;
parasites a viable option for treating an overac-
mous variation that fuels natural selection.means
metabolism Understanding
how fast viral evolution al-
a person A. Energy Allows Cells to Do Life’s Work
tive immune system. “reduction” means electrons are added. As you will see, photosynthesis strips
lows researchers to stay a step ahead, producing vaccines that protect In the future, biologists hope to apply evolutionary theory to answer
burns food. But biochemists define
against the new strains of the flu that arise each year. Unfortunately, scientists
Physicists What’s the Point?
electrons
define energy
These
from
as the the oxygen
APPLIED
ability
electronsisreduce
atoms in H2is,
to do work—that
questions about everything from aging to mental illness. A thorough
the carbon
O (i.e.,
to move
under-
in CO2. Because
the oxygen
matter. atoms are oxidized).
oxygenonatoms attract electrons
O2 Sugar
metabolism as all of the chemical
have not been able to develop vaccines against all viral infections. The rapid This idea,
standing of as
thisabstract
powerfulas it sounds,
process fundamental
will undoubtedly lead totoa healthier
biology. Life depends
future.
reactions that build and break down more strongly than do carbon atoms (see chapter 2), moving electrons from oxy-
rearranging atoms and trafficking substances across membranes in precise Figure 5.6into
Overview of Photosynthesis. In the light
©Comstock Images RF Metabolism describes all theto chemical reactions in a cell. Because for this reaction is, oftransport
course, fatty acids cells become more numerous afterreactions,
molecules within any cell. How are ways. These intricategen movementscarbonrepresent
requires energy.
work, and Thethey energy source
require energy. pigment molecules captureproviding
light energycells
and transfer it to molecules
our cells always lose energy
light. i asredox
heat,reactions,
they require
sectionconstant
4.2B en- one to two weeks of exercise, with easier
these two meanings related? Although it may seem strange to think of a “working” cell, all organisms
ergy input to continue fueling their reactions. So the familiar access toofenergy.
ATP and NADPH. The carbon reactions use this energy to build
Interlocking networks of metabolic reactions supply the do tremendous amounts of work on a microscopic scale. For example, a plant sugar may
molecules out of carbonmetabolism.
dioxide.
definition of metabolism—how fast a person burns calories in Caffeine also accelerate Although caf-
cell assembles glucose molecules into long cellulose fibers, moves ions across
energy that every cell needs to stay alive. In humans, teams food—relates to the rate 5.4 at which Mastering Concepts
cellular reactions are occur- feine contains zero calories, many people can attest to the
its membranes, and performs thousands of other tasks simultaneously. A
of metabolizing cells perform specialized functions such ring. What can you do to make your cells use the energy in food “energy boost” that it provides. Caffeine increases the release
gazelle grazes on a plant’s 1. tissues
What happens
to acquire inenergy
each of
thatthe twoenable
will main itstages
to do of
its photosynthesis?
as digestion, muscle movement, hormone production, and more quickly? of fatty acids into the blood and raises the heart rate, giving
own cellular work. A crocodile
2. Where eatsinthat
thegazelle for thedoes
chloroplast sameeachreason.
stage occur?
countless other activities. It all takes a reliable energy supply— Exercise
The totalspeeds
amountup the body’s
of energy in anyenergy
object ismetabolism in
the sum of energy’s two forms: cells quick access to energy reserves. However, studies have
food, which each of us “burns” at a different rate. several ways.
potential Immediately
and kinetic (figure 4.1)after exercise,
. Potential energycellsis stored
work energy
to available to shown that getting too little sleep (a side effect of excess caf-
This chapter describes the fundamentals of metabolism, rebuild ATPAand
do work. other atenergy
bicyclist the topreserves, so caloricpotential
of a hill illustrates demands energy, as does a feine) disturbs normal metabolism.
including how cells organize, regulate, and fuel the chemical arecompressed
high. Also,spring.
body The
temperature remains elevated for hours
covalent bonds of molecules, such as the ingredients Finally, metabolism slows down when the body receives too
reactions that sustain life. after exercise,
in your energyspeeding chemical
bar, contain 5.5 The Light Reactions Begin
a formreactions
of potential and contributing
energy to
called chemical energy.
©Corbis RF
few calories. Hormones then signal the body that it is entering a
increased metabolism.
A concentration Regular
gradient exercise
is another formalso increases
of potential the size
energy of
(see section 4.5). starved state. In response, cells begin to conserve energy via several
muscle cells, which
i covalent bonds,require
section more
Photosynthesis
2.2C energy than fat cells even when at mechanisms. One way to keep your metabolism high is therefore to
rest. Exercise also increases
Kinetic energy Ais the
plantenergy theofin
placed abundance
motion;
a dark any of enzymes
moving
closet literallyobject and other this
possesses
starves. Without maintain
light, your
the plant blood sugar level by eating multiple small, healthy meals
cannot
proteins
form ofthat regulate
energy. energyATP
The bicyclist
generate metabolism.
coasting down the
or NADPH. For example,
hill
And in figure
without proteins that
4.1 critical
these sources throughout
demonstrates of energy andtheelec-
day.
kinetic energy, as dotrons,
molecules inside
the plant a cell.
cannot In fact,
produce all oftothe
sugars chemical
feed reac-its stored reserves are
itself. Once
tions that sustain lifegone,
rely on
thecollisions
plant dies.between moving
The plant’s molecules.
life thus dependsThe colder
on the light reactions of photo-
an object feels, the slower thewhich
synthesis, movement of the
occur in its membranes
atoms and molecules; this is
of chloroplasts.
why many cells die if conditions are too chilly.
Calories are units used to measure energy. One calorie (cal) is the amount
Burning
Figure 4.1 Potential and Kinetic cover
Questions topics
Energy. (a) A bicyclist at that Burning
of energy required to raise Question
the temperature of 1 gram 5.1of water from 14.5°C to B. ATP Represents Short-Term Energy Storage
CHAPT ERenergy
S U content
MMAofRY
students wonder about.
the top of a hill has potential energy, which can be converted to 15.5°C. The food, however, is usually measured in kilocalories
•
ATP is too unstable for long-term storage. Instead, cells store energy as fats
kinetic energy as the cyclist coasts down. (b) A compressed spring Why do leaves change colors in the fall? and carbohydrates.
4.1 All Cells Capture and Use Energy
has potential energy, which is released as kinetic energy when
Every
the spring ischapter
released. (c)in the book
Chemical energy is answers one or
a form of potential moreAllows Cells toMost
A. Energy leaves Work
Do Life’s are green throughout a plant’s growing season, although there
are exceptions; some ornamental plants, for example, have yellow 4.4or Enzymes Speed
time as a spectacular autumn display. These pigments soon disappear as well,
and the Reactions
dead leaves turn brown and fall to the ground.
energy, which is released when the molecule’s bonds break.
Burning Questions, encouraging readers to • Energy is the ability to do work. Potential energygreenis stored energy,
and ask
purple foliage. The familiar color comes from chlorophyll A.
a, Enzymes Bring ReactantsSpring brings a flush of fresh green leaves. The energy to pro-
Together
kinetic energy is action.
the most abundant pigment in photosynthetic plant parts. duce the foliage comes from glucose the plant produced during the
questions ofOntheir hill: own. I ask my students to write • Energy is measured in units called
But calories.
the leaf One
also hasfood Calorie
other is
photosynthetic pigments. • Enzymes are organic molecules (usually proteins) that speed biochemical
last growing season and stored as starch. The new leaves make
reactions by lowering the activation energy.
High potential energy 1000 calories, or 1 kilocalorie.
Carotenoids contribute brilliant yellow, orange, and red hues. food throughout the spring and summer, so the tree can grow—
down a Burning Question on the first day B. Life Purple pigments, such as anthocyanins, are not photosyn- •
of Requires Energy Transformations Substrate molecules fit into the enzyme’s activeand
both above the ground site.
below—and produce fruits and seeds.
thetically active, but they do protect leaves from damage by B. Many Factors Affect Enzyme As Activity
the days grow shorter and cooler in autumn, the cycle
class. I answer all of them during the semester, • Energy cannot be created or destroyed but only converted to
Glucoseother forms.
molecule:
to the so they •
ultraviolet radiation. A reaction product may will continue,
temporarily shut and
downthe its
colorful
own pigments
synthesiswill again participate
whenever
whenever a relevant topic comes Coastingupdownin • Every reaction increases entropy
hill:class.
(disorder)
Carotenoids are and
less loses
High
abundant
potential
heatthan
energy energy
chlorophyll, in one of nature’s great disappearing acts.
its levels rise. Such negative feedback may occur by competitive
Energy is released
environment. usually remain invisible to the naked eye during the growing sea-
as kinetic energy. son. As winter approaches, however, deciduous plants prepare to inhibition or noncompetitive
Submitinhibition.
your burning question to

4.2 Networks of Chemical Reactions

shed their leaves. Anthocyanins accumulate while chlorophyll degrades,
Sustainpigments
Life reveal their colors for a short
•
Enzymes have narrowmarielle.hoefnagels@mheducation.com
ranges of conditions in which they function.
and the now “unmasked” accessory (leaves): ©Carlos E. Santa Maria/Shutterstock RF
• Metabolism is the sum of the chemical reactions in a cell. 4.5 Membrane Transport May Release
A. Chemical Reactions Absorb or Release Energy Energy or Cost Energy
viii • Compressed
In reactions spring:
that require energyExtended
High potential
The Light Reactions Begin Photosynthesis
spring:
input, the products have more energy
Energy is released Stored energy is •
The Carbon Reactions Produce Carbohydrates C3, C4, and CAM Plants
Membranes have selective permeability, which means they admit only
than the reactants. Conversely,
energy as in reactions
kinetic energythat
and release energy,when
released the the some substances.
products have less energy than transferred
the reactants.
to ball. bonds are broken.
a. B. Linked Oxidation
b. and Reduction Reactions Form c. Electron
Transport Chains
69 Miniglossary | Energy of Life
 168 UNIT 2 DNA, Inheritance, and Biotechnology

9.5 Meiosis Generates Enormous Variability

CHAPTER SUMMARY
A. Crossing Over Shuffles Alleles

108 UNIT
The 1chapter
Science, Chemistry,
summary and Cells 9.1 Why Sex?
• Asexual reproduction is reproduction without sex. Sexual reproduction
• Crossing over, which occurs in prophase I, produces variability when
portions of homologous chromosomes switch places. After crossing over,
produces offspring by mixing traits from two parents. the chromatids carry new combinations of alleles.

highlights key points and • Asexual reproduction can be successful in a stable environment, but a
changing environment selects for sexual reproduction.
B. Homologous Pairs Are Oriented Randomly During Metaphase I
• Every possible orientation of homologous pairs of chromosomes at
metaphase I is equally likely. As a result, one person can produce over

terminology from the chapter. 9.2 Diploid Cells Contain Two Homologous Sets
of Chromosomes
8 million genetically different gametes.
C. Random Fertilization Multiplies the Diversity

• • Because any sperm can fertilize any egg cell, a human couple can produce
a. Alcoholic fermentation Diploid cells have two full sets of chromosomes, one from each parent.
A karyotype is a chart that displays all of the chromosomes from one cell. over 70 trillion genetically different offspring.

Chapter summary illustrations help •

6.8 Fermenters Acquire ATP Only
In humans, the sex chromosomes (X and Y) determine whether an
individual is male or female. The 22 homologous pairs of autosomes do
not determine sex.
• Identical (monozygotic) twins arise when a zygote splits into two embryos.
Fraternal (dizygotic) twins develop from separate zygotes.

students see the big picture.

Glycolysis • 9.6 Mitosis and Meiosis Have Different Functions:

in Glycolysis
Homologous chromosomes share the same size, banding pattern, and

ATP
centromere location, but they differ in the alleles they carry.
•
A Summary
Chapter 10 Patterns of Inheritanc
Glucose 2 9.3 Meiosis Is Essential in Sexual Reproduction •
Mitotic division produces identical copies of a cell and occurs throughout life.
Meiosis produces genetically different haploid cells. It occurs only in
• Meiosis halves the genetic material to produce haploid cells. Fertilization specialized cells and only during some parts of the life cycle.

2 NAD+
Most of the known organisms onSometimes
Earth, Occurincluding
occurs when gametes fuse, forming the diploid zygote. Mitotic cell division
9.7 Errors
produces the body’s cells during growth and development.
Figure 9.13 summarizes the events of a sexual life cycle.
In Meiosis humans, use aerobic cellular
A. Polyploidy Means Extra Chromosome Sets
respiration. Nevertheless, • life thrives without O2 in waterlogged soils, deep Polyploid cells have one or more extra sets of chromosomes.
2 Pyruvate B. Nondisjunction Results in Extra or Missing Chromosomes

7. In the ABO blood type system, the enzymes that link puncture
molecules wounds,A andsewage
B • treatment plants, and your own digestive tract, to Nondisjunction is the failure of homologous chromosomes or sister

PULLof IT O2, TOG ETHinER

chromatids to separate, causing gametes to have incorrect chromosome

to red blood cells each consist of 354 amino acids;name their just a few places.
differ In the absence the microbes these habitats
numbers. A sex chromosome abnormality is typically less severe than an
2 NADH sequences DEVELOPMENT
incorrect number of autosomes.
MEIOSIS

by four amino acids. The allele encoding the i allelegenerate

has a ATP using
frameshift
FERTILIZATION
metabolic pathways that are anaerobic (meaning they do
2
not use O2). Fermentation is one such pathway.
GAMETE FORMATION

mutation; it therefore encodes a nonfunctional protein.

Ethanol Combine the Diploid (2n)
Haploid (n)

concepts of chapters 7 and 10 to diagram how blood cells acquire their In organisms that use fermentation,
HAPLOID glycolysis
CELLS still yields DIPLOID two ATPs,
CELLStwo
2 CO2 Diploid (2n = 4)

ABO phenotype, starting with transcription of• the NADHs, IA, IB, or iand two molecules of pyruvate per molecule of glucose. But the
Figure 9.13 Sexual Life Cycle Events.

allele and
Somatic cells do not participate in reproduction, whereas diploid germ cells
MEIOSIS I

NADH does not donate its electrons to an electron transport chain, nor is the
produce haploid sex cells.

Input endingOutput with the surface of the red blood cells.9.4 In Meiosis, DNA Replicates Once, but the
pyruvate
Nucleus Divides Twice further oxidized.
2 8. Calico2cats have large patches of orange and black • fur;Instead,
in tortoiseshell
The events of meiosis ensure that gametes are haploid and genetically
in fermentation, electrons from NADH reduce pyruvate. This
variable (figure 9.14).
•
cats, the patches are smaller. In which type of• cat does X inactivation
During interphase, which precedes meiosis, the cell grows and copies its Haploid (n = 2) Haploid (n = 2)

2 CO 2 process DNA.
regenerates NAD+, which is essential for glycolysis to continue. But
Spindle proteins move the chromosomes throughout meiosis. Homologous
MEIOSIS II MEIOSIS II

occur earlier
2 NAD+in development? How do you know? fermentation producesProtein
pairs of chromosomes align during prophase I, line up double-file at the
no additional ATP. This
2 NADH and pathway is therefore far less
cell’s center during metaphase I, then split apart during anaphase I.
Processing
contain one
The chromosomes arrive at the poles in telophase I, and the cell often Degradation containSome twoproteins
9. Explain the following “equation”: • efficient than respiration.
divides (cytokinesis).

must be Not surprisingly,

altered before of fermentation
set they
The two products of meiosis I each enter meiosis II. The chromosomes
become functional
condense during prophase II. During metaphase II, they line up single-file
is most ofcommon
(figure
sets 7.11, step
among microorganisms 6).that live ininsulin,
Producing sugar-rich environments
for example,
at the cell’s equator. The sister chromatids are separated in anaphase II,
requireswhere a precursorfoodprotein
is es- to
Genotype + Environment = Phenotype
Four haploid cells (n = 2)
and the chromosomes arrive at the poles in telophase II. Cytokinesis then
b. Lactic acid fermentation sentially unlimited. be cut in two places. If these modifications fail to occur, the insulin Autosomes Ot
occurs once more to yield four haploid cells. Figure 9.14 Summary of Meiosis.

Many of the microbes proteinthatcannot function.

live in human intestines make their entire may beliving
tr
Glycolysis Chromosomes
by fermentation. One In addition,
example is a to do its job, a protein
disease-causing must(an
protist move from thethat
amoeba) ribosome to
Glucose 2 ATP where the cell needsNew
X or Y (sex
SCI E N TI F I C LI TE RACY causes a form of dysentery in humans. it. For example,
Others, Scientific
includinga protein
the secreted
Literacy
bacterium in milk must be escorted
Esch- chromosomes) DNA
to the Golgi apparatus andinclude be packaged
multiple forlinked
export (see figure 3.11). A gene is
2 NAD+ erichia coli, use O2 when it is availablequestions but switch to help studentswhen it is
fermentation
effectively silenced if its product never moves to the correct destination. Finally,
a.

2 PyruvateReview Burning Question 10.1, which describes the inheritance pattern like
not. Most multicellular organisms,
RNA, not all however,
understand
proteins require
are equally toostable.
muchSome
where energy
biology to rely on
are degraded shortly after they
of the metabolic disease called PKU. Today, genetic testing fermentation for manyexclusively.form, whereas othersintersects persist longer.Genes with ethics, politics,
2 NADH Of parents
the manyhave fermentation pathways that exist, onetoof the most familiar
disorders is relatively easy and inexpensive. Do prospective A human cell mayand express
social hundreds
issues. thousands of genes at once. Unraveling
an obligation to determine how likely they are to produces
conceive a ethanol
child with (an
a thealcohol).
complex regulatory
In mechanisms
alcoholic that control the pyruvate
fermentation, expression ofiseach gene is an
2 have alternative
enormous
and CO2challenge. Biologists now have thetotechnology to begin+ navigating this
some possible drawbacks converted of learningtomore ethanol , while NADH isversions
oxidized called produce NAD
b.
genetic disorder? What Lactic are
acid about
regulatory maze. The work has just begun, but the payoff will be a much better
or lactate (figure 6.10a) . Alcoholic fermentation produces
one’s own genetics? What are some possible advantages to oneself and to understanding of cell biology, along with many new medical applications. The wine from grapes, beer
from barley, and cider from apples. Dominant
society? Output
Input same research may also help scientists understand how external influences on gene
In lactic acid fermentation, a cell uses
expression contribute NADHAlleles
to complex to reduce
traits, such pyruvate, mayy be ebut in see Burning
as homosexuality;
2 2 this case, the products are NAD+7.1. and lactic acid or its close relative, lactate (figure Recessive
Question A A A A
6.10b). The bacterium Lactobacillus, for example, B Bfermentsbthe b lactose in milk,
2 NADH
Write ItGE Out Nand 2 NAD+
E TI C S P ROConcepts
Mastering B LE M Squestions areproducing useful
the lactic acid that gives yogurt its sour taste. typically
Fermentation also occurs 7.5 Masteringin human muscle Concepts cells. During vigorous exer- encodes
for student review or as short in-class writing assignments. nonfunctionalc.
Figure 6.10 See Fermentation.
the How to Solve a Genetics ATP
In fermentation, comessection
Problem at thecise,
only from end of muscles work so
this chapter forstrenuously
1. Which steps that in theygene consume
g expression
g their available
G require G energy? oxygen
I compile
glycolysis. them
step-by-step
(a) Yeasts into aethanol
list ofand
guidance.
produce Guided
carbonReading
dioxide by alcoholic that
Questions supply.
help In this “oxygen debt” 2. Why condition,
do cellsthe muscle
regulate cellsgenes
which can acquire
are expressed?ATP only
students1.
fermentation; focus
one on
possiblematerial
product I
is cover in
champagne.class.
(b) I also
Lactic use
acid them from
as glycolysis. The cells 3. use How lactic
doesacid fermentation
a repressor protein helpto generate
regulate NAD
+
so
the expression of a Protein
In rose bushes, red flowers (FF or Ff) are dominant to white flowers (ff). Figure
that glycolysis can continue. Lactate
bacterial concentrations
operon? therefore rise. After the
discussion
fermentation questions
occurs in some in Action
bacteria and,Centers, where
occasionally, in students
mammalian
A true-breeding red rose is crossed with a white rose; can come two for
flowers of the if twoo different ent if t
two identical
ide and other
muscle cells. exercise, when the circulatory 4. Explainsystem how catches
epigenetic up with the muscles’
modifications changedemand for
the likelihood of
additional Fhelp
1
with course
generation material. crossed. What will O
are subsequently be, the livermostcellscommon
convert lactatetranscription.
back Heterozygous
to pyruvate. Mitochondria then process the
factors, in
Photos: (a): ©Brand X Pictures/PunchStock RF; (b): ©Corbis RF 2 Homozygous (c) DNA b
genotype of the F2 generation? pyruvate as usual. 5. What is the role of transcription factors in gene expression? polymera
2. In Mexican hairless dogs, a dominant allele confers hairlessness. One common misconception aboutGgintense exercise is GG thatorlactic
gg
acid
However, inheriting two dominant alleles is lethal; buildup
the fetus causes
diesthebefore
pH to drop in muscle cells, provoking soreness a day or two
birth. Suppose two dogs that are heterozygous for the hair allele mate. is a myth. Instead, microscopic tears in muscle tissue
later. This idea, however,
Predict the genotypic and phenotypic ratios of theare now thought
puppies that areto be the culprit responsible for delayed
born. may bemuscle soreness.

Figureand It Out questions reinforce 7.6 Mutations Change DNA

chapter
3. A species of ornamental fish comes in two colors; red is dominant Genotype
concepts Aand
mutation is any change
typically have in a cell’s DNA sequence. The change may occur
numeric
gray is recessive. Emily mates her red fish with a gray fish. If 50 of the
in a gene or in a regulatory region GG, Gg, suchorasgg
a promoter. influence
Many people thinkPhenotype
that
Figure100 babies are red, what is the genotype of Emily’s fish?
It Out answers (supporting
6.8 Mastering Concepts student math skills).
mutations are always harmful, perhaps because some of them cause such dra-
Compare4. theTwo lizards
number have green
of molecules of ATPskin and from
generated large 100dewlaps
glucose (genotype GgStudents
1. How Dd).
many If ATP
theymatic
can changes
work
molecules on per(figure
these 7.13). Although
in
glucosesmalldoes someinmutations do cause illness, they
groups,
fermentation
Environment
mate andaerobic
molecules undergoing 32 offspring
respirationare born,
versus how many of the offspring
fermentation. are
produce?expected also provide the variation that makes life interesting (and makes evolution
class, or inpossible).
Action Centers. Most could easily be
to be homozygous recessive for both genes? (Assume2.thatWhat theused
traits
areas assort
clicker
two Toquestions
examples theascookbook
well.Fertpathways?
of fermentation
continue analogy introduced earlier, a mutation in a gene
ilizer
independently.)
Answer: 3600 (theoretical yield) for aerobic respiration; 200 for fermentation.
is similar to an error in a recipe. A small typographical error might be barely
5. A fern with genotype AA Bb Cc dd Ee mates with another fern with noticeable. A minor substitution of one ingredient for another might hurt (or
improve) the flavor. But serious errors H2Osuch as missing ingredients or truncated
genotype
Cells Use Energy in Food to Makeaa
ATPBb CC Dd ee. What
Cellular proportion
Respiration: of the offspring will
Three Processes be Produce
Mitochondria Most ATP Glycolysis Breaks Down Glucose to Pyruvate
instructions are likely to ruin the dish.
heterozygous for all genes? (Assume the genes assort independently.) Figure 10.28 Pull It Together: Patterns of Inheritance. ix
a.
Hint: Use the product rule.
6. In Fraggles, males are genotype XY and females are XX. Silly, a male A. Mutations Range from Silent
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Changes by Chapter
Chapter 1 (The Scientific Study of Life):
Developed new miniglossary comparing sexual and asexual reproduc-
tion; revised figure 1.12 to include experimental design.
Chapter 2 (The Chemistry of Life):
Clarified definitions in miniglossary of matter; added periodic table en-
try and definitions to figure 2.4; developed new table 2.3 to summarize
water’s characteristics; improved illustration of cellulose in figure 2.19
to show hydrogen bonds; omitted vitamin D as an example of a steroid;
updated nutrition label in What’s the Point? Applied to conform with
latest FDA guidelines; wrote new Investigating Life on defensive chemi-
cals in ants; simplified and improved summary figures for clarity.
Chapter 3 (Cells):
Clarified functions of free-floating and membrane-bound ribosomes.
Added the following ebook-specific learning tools: table summarizing
cell junctions; table summarizing the structures in eukaryotic cells.
Chapter 4 (The Energy of Life):
Explained how kinetic energy relates to an object’s temperature; made
small changes to section 4.1 to clarify the passage on energy transforma-
tions; clarified definition of negative feedback; improved illustrations of
plant cells in figure 4.17. Added the following ebook-specific learning
tool: table showing types of energy.
Chapter 5 (Photosynthesis):
Expanded miniglossary of leaf anatomy; revised caption of figure 5.5 to
clarify components of photosystems (based on SmartBook user data);
improved description of electron transport chain in the light reactions;
clarified passage on C4 pathway; wrote new Investigating Life on solar-
powered salamanders. Added the following ebook-specific learning
tool: table summarizing photosynthetic pigments.
Chapter 6 (Respiration and Fermentation):
Changed chapter title to complement “Photosynthesis” chapter title;
revised caption of figure 6.2 to include the role of electron carriers
(based on SmartBook user data); clarified in several places through-
out the chapter that proton is synonymous with hydrogen ion (H+);
improved figure 6.9 to show how nitrogen from amino acids becomes
a metabolic waste (based on SmartBook user data). Added the fol-
lowing ebook-specific learning tools: table showing where respiration
occurs in prokaryotes and eukaryotes; table comparing respiration and
photosynthesis.
xii
Chapter 7 (DNA Structure and Gene Function):
Omitted the implication that transcription is a stage of protein synthesis
(i.e., proteins are produced only in translation); added new miniglos-
sary to help students understand the relationships between nucleotides,
genes, chromosomes, and genomes (based on SmartBook user data);
clarified that each cell contains many different tRNA molecules; im-
proved figure 7.8 by zooming in on the codon/anticodon interaction
(based on SmartBook user data); added photo of translation to comple-
ment the translation art in figure 7.9; expanded coverage of epigenetics,
both in the main narrative and in Burning Question 7.1; added Ebola
and Zika viruses to table 7.2, which lists viruses that infect humans;
improved viral replication figure 7.18 to show receptors on the entire
cell surface; wrote new subsection within section 7.8 explaining how
influenza causes symptoms; improved and expanded miniglossary of
viruses; clarified Investigating Life section and reworked figure 7.A to
include evolutionary tree; improved summary figures 7.25, 7.26, and
7.28; added summary table 7.3 comparing viruses and cells. Added the
following ebook-specific learning tools: table describing three types of
RNA; tables summarizing the stages of transcription and translation
(based on SmartBook user data); table summarizing regulated points in
protein production.
Chapter 8 (DNA Replication, Binary Fission, and Mitosis):
Improved definitions in miniglossary of cell division; used the words
align and line up consistently (in referring to chromosome move-
ments) to conform with changes in chapter 9; modified Burning
Question 8.1 to include cancer cells; briefly mentioned newer cancer
therapies (such as immunotherapy); wrote new Investigating Life es-
say that explains how evolutionary principles can be used in planning
chemotherapy; added miniglossary of cell division terms to chapter
summary.
Chapter 9 (Sexual Reproduction and Meiosis):
Used the words align, line up, and orient consistently (when referring to
chromosome movements); explicitly listed in the narrative three mech-
anisms that generate genetic variability and added new Figure It Out
problem in section 9.5 (based on SmartBook user data); defined recom-
binant and parental chromatids to improve consistency with chapter 10
and added both terms to a miniglossary; revised figure 9.15 (Pull It
Together) to improve the connections among the terms.
Chapter 10 (Patterns of Inheritance):
Clarified some headings and subheadings to better reflect chapter con-
tent; changed alleles for yellow and green seeds from G and g to Y and

y in art and narrative; clarified cookbook analogy by relating it back
to chapter 7; added an explanation for why certain alleles are reces-
sive; clarified that cells with incorrect chromosome numbers may not
have exactly two alleles per gene; reworked Burning Question 10.1 to
focus more on the warning label; improved miniglossary of genetic
terms by adding some terms and revising others; improved illustration
of test cross (figure 10.8); clarified explanation of the product rule;
more clearly distinguished recombinant chromatids from recombinant
offspring (based on SmartBook user data); clarified explanation of
ABO blood group system; improved explanations of pleiotropy and
many gene/one phenotype situations; connected environmental effects
on gene expression to epigenetics; reworked figure 10.25 to add the
frequency of each possible skin color phenotype; updated Investigating
Life essay to include two-toxin strategy for slowing the evolution of
Bt-resistant insects; added new summary figure 10.26 to show the con-
nection between mutations and Punnett squares; improved summary
figure 10.27 to better illustrate the light bulb analogy. Added the fol-
lowing ebook-specific learning tools: new figure depicting the P, F1,
and F2 generations (based on SmartBook user data); new summary fig-
ure showing a timeline that includes the main genetics-related events
described in the chapter.
Chapter 11 (DNA Technology):
Expanded passage on ethical issues related to transgenic organisms;
added content on high-throughput DNA sequencing methods; updated
data on DNA exonerations; added content on cloning in plants, including
a new illustration (figure 11.10); added new subsection to section 11.4
on CRISPR-Cas9, including a new illustration (figure 11.15).
Chapter 12 (Forces of Evolutionary Change):
Improved figure 12.8 to show the connection between natural selec-
tion and DNA; added table listing misconceptions about evolution and
showing how a biologist would address each (based on SmartBook user
data); added new Burning Question about whether there is a “pinna-
cle of evolution”; modified figure 12.13 to make the Hardy–Weinberg
equations more prominent; modified figure 12.14 to include three phe-
notypes for directional selection; clarified the distinction between the
bottleneck effect and natural selection; wrote new Investigating Life on
antibiotic-resistant bacteria from livestock; reworked figure 12.24 (Pull
It Together) to make it more informative.
Chapter 13 (Evidence of Evolution):
Added the proposed Anthropocene epoch to geologic timescale
(figure 13.2); improved figure 13.15 for clarity and to add a lemur ex-
ample. Added the following ebook-specific learning tools: miniglossary
of estimating a fossil’s age; miniglossary of comparative anatomy.
Chapter 14 (Speciation and Extinction):
Made small changes to several evolutionary trees to ensure consistent
use of the word ancestor; added terms to miniglossary of speciation
and extinction; revised Why We Care 14.1 to add new illustration and
Changes by Chapter xiii
information about why extinctions are important; wrote new Burning
Question 14.2 (“Did rabbits come from frogs?”); clarified the relation-
ship between genus and species (based on SmartBook user data); wrote
a new Investigating Life essay on plant “protection rackets.” Added the
following ebook-specific learning tools: miniglossary of reproductive
barriers; new figure showing multiple ways to depict the same evolu-
tionary relationships.
Chapter 15 (Evolution and Diversity of Microbial Life):
Made small changes to several evolutionary trees to ensure consistent
use of the word ancestor; clarified that the outer membrane is consid-
ered part of the cell wall in bacteria; added miniglossary of prokaryote
anatomy and revised miniglossary of prokaryote diversity; reworked
figure 15.12 to clarify aerobic and anaerobic habitats; foreshadowed
in section 15.2C that proteobacteria and cyanobacteria are related to
the bacteria participating in endosymbiosis, then returned to that idea
in section 15.3A and in figure 15.19; clarified explanation of nitrogen
fixation; referred specifically to human microbiota; wrote new Burning
Question 15.2 about areas on Earth without life; added new figure 15.21
to illustrate the evolution of multicellularity; clarified basidiomycete
life cycle in figure 15.35; added illustration (figure 15.37) showing
fungi in everyday life; based on heat map data, clarified the differences
between arbuscular mycorrhizae and ectomycorrhizae and between
endophytes and mycorrhizae; revised figure 15.40 to show resources
exchanged between the partners in a lichen. Added the following
ebook-specific learning tools: miniglossary of types of algae; table of
plasmodial and cellular slime mold life cycles; miniglossary of types
of protozoa; miniglossary of fungal anatomy; miniglossary of fungal
partnerships.
Chapter 16 (Evolution and Diversity of Plants):
In section 16.1’s narrative, clarified relationship between zygote and
sporophyte (based on SmartBook user data); reworked figure 16.10
to clarify that fern gametophytes do not self-fertilize; in section 16.4,
clarified that ovules develop into seeds in narrative and corresponding
art. Added the following ebook-specific learning tool: table listing key
plant-adaptations.
Chapter 17 (Evolution and Diversity of Animals):
Clarified arrows depicting gastrulation in figure 17.5; added new mini-
glossary of arthropod diversity; modified figure 17.30 to better high-
light the three groups of primates; clarified that Ardipithecus species are
extinct and mentioned the recently discovered H. naledi fossils; added
evolutionary tree to figure 17.C (Investigating Life).
Chapter 18 (Populations):
Updated demographic data for the world population in art and narrative;
improved explanation of the demographic transition and added new il-
lustration (figure 18.14); updated information on China’s one-child pol-
icy. Added the following ebook-specific learning tool: miniglossary of
population growth.
xiv Changes by Chapter
Chapter 19 (Communities and Ecosystems):
Made small corrections to convection cells in figure 19.4; added new
figure 19.14 to illustrate mutualism and commensalism; updated data
about mercury in tuna; added new Burning Question 19.2, comparing
bottled water with tap water; clarified the meaning of the word eutro-
phication; wrote new Investigating Life essay on monarch butterfly mi-
gration.
Chapter 20 (Preserving Biodiversity):
Added the term Anthropocene and a new illustration (figure 20.2) illus-
trating where human impacts on the biosphere are most intense; men-
tioned the acronym HIPPO at the start of the chapter; added landfills
as a source of water pollution; improved narrative and figure 20.9 ex-
plaining acid deposition; updated narrative and improved figure 20.10
explaining greenhouse effect; added graph to figure 20.12 showing de-
cline in the extent of Arctic sea ice; added advice for people who fish to
Burning Question 20.5; clarified figure 20.19 (Pull It Together). Added
the following ebook-specific learning tools: table listing consequences
of global climate change; miniglossary of pollution.
Chapter 21 (Plant Form and Function):
Added art of shoot apical meristem to figure 21.15; clarified that
axillary bud and lateral bud are synonymous; wrote new Burning
Question 21.2 about controlled burns; clarified that hormones are pres-
ent in xylem sap; added photo of a wilted plant (figure 21.21) and a
corresponding description of why plants wilt when soil is too dry.
Chapter 22 (Reproduction and Development of
Flowering Plants):
Clarified passage on flower structure; added miniglossary of the angio-
sperm life cycle (based on SmartBook user data); clarified passage on
coevolution between flowers and pollinators; clarified role of cotyledons
in eudicots and monocots; added photo of coconut to figure 22.9 to show
a water-dispersed fruit; added new Why We Care 22.1 on “talking plants”;
annotated figure 22.15 to show how photoperiod affects flowering time.
Chapter 23 (Animal Tissues and Organ Systems):
Modified art for simple columnar epithelium in figure 23.2 to better
match the accompanying photo; added new Burning Question 23.1 on
the body’s reaction to food poisoning; clarified narrative and figure 23.8
to identify the stimulus, sensor, control center, and effector(s); added
miniglossary of negative feedback; clarified definition of ectotherm.
Added the following ebook-specific learning tools: miniglossary of ani-
mal anatomy and physiology; miniglossary of animal tissues; miniglos-
sary of temperature homeostasis.
Chapter 24 (The Nervous System and the Senses):
Added new miniglossary of neuron anatomy; clarified definitions of
membrane potential and resting potential (based on SmartBook user
data); clarified why the inside of a resting neuron has a net negative
charge; labeled the voltage meters in figures 24.4 and 24.5 to clarify
their function; added context to figure 24.13 illustrating the blood–brain
barrier; added information about concussions to section 24.6; wrote new
Burning Question 24.2 explaining whether we use 10% of our brain;
improved figure 24.19 by showing context for the olfactory bulb and
olfactory epithelium; added new miniglossary of vision; clarified in fig-
ure 24.24 that the overlying membrane in the cochlea does not consist
of cells; expanded description of cochlear implants. Added the follow-
ing ebook-specific learning tools: miniglossary of membrane potentials;
miniglossary of smell and taste; miniglossary of hearing.
Chapter 25 (The Endocrine System):
Added paragraph about negative feedback loops to section 25.1; clari-
fied that internal hormone receptors may be in the cytosol or in the
nucleus and elaborated that steroid hormones may either stimulate or
inhibit protein production (based on SmartBook user data); completed
descriptions of effects of ADH and oxytocin in figure 25.4; reworked
Burning Question 25.1 to focus on endocrine disruptors; adjusted labels
in figure 25.7 to add the role of the hypothalamus as a sensor; adjusted
labels in figure 25.9 to add the role of the pancreas as a sensor; reworked
figure 25.11 showing the correlation between obesity and diabetes; re-
worked the What’s the Point? Applied box to focus on chronic stress.
Added the following ebook-specific learning tool: summary table of
hormones and their functions.
Chapter 26 (The Skeletal and Muscular Systems):
Clarified illustration of scoliosis (figure 26.3); revised figures in sec-
tion 26.4 for clarity and improved page layout; improved description of
the sarcomere and of the cross bridges in the sliding filament model;
added a paragraph about sports balms to Burning Question 26.2; added
miniglossary of the muscular system to the chapter summary. Added
the following ebook-specific learning tool: table outlining the steps of
muscle contraction.
Chapter 27 (The Circulatory and Respiratory Systems):
Improved consistency between ABO blood type passage in section 27.1
and related material in section 10.6; clarified the roles of the pulmo-
nary and systemic circulation, especially with regard to O2 and CO2
(based on SmartBook user data); wrote new Burning Question 27.3
on extreme exercise; added terms to the miniglossary of circulation;
clarified blood pressure monitors in figure 27.13. Added the following
ebook-specific learning tools: miniglossary of the heartbeat; miniglos-
sary of breathing.
Chapter 28 (The Digestive and Urinary Systems):
Improved consistency in the use of ions and salts throughout the chapter
(based on SmartBook user data); added information about how a high-
fiber diet lowers cholesterol and helps regulate blood sugar; updated
figure 28.4 to reflect new nutrition label regulations; added Burning
Question 28.1 about fad diets; clarified that the stomach does not absorb
the proteins it begins to digest (based on SmartBook user data); clarified
illustration of the large intestine (figure 28.19).
 Changes by Chapter xv

Chapter 29 (The Immune System):

Improved explanation of lymph; clarified narrative, figure 29.7, and fig-
ure 29.10 to show clonal selection for both T cells and B cells; added
paragraph about cancer immunotherapy; reworked figure 29.13 illus-
trating the effects of immunodeficiencies; clarified that mast cells and
basophils participate in allergies; added new Burning Question 29.2
about tick-transmitted meat allergies; added narrative about “retraining”
the immune system in children with peanut allergies.

Chapter 30 (Animal Reproduction and Development):

Clarified description of external fertilization; improved explanation
of how oocytes enter uterine tubes; changed sexually transmitted
diseases to sexually transmitted infections to recognize that not all
infections lead to visible disease symptoms; added a labeled sperm
cell to figure 30.12 to remind students where the acrosome is (based
on SmartBook user data); clarified two descriptions in table 30.4; im-
proved the explanation and illustration (figure 30.15) of the placenta’s
structure and function; added labels to clarify the stages of childbirth
in figure 30.18; added new summary figure 30.20 to illustrate the paths
of sperm and egg cells. Added the following ebook-specific learning
tools: miniglossary of embryonic support structures; new summary
table showing a timeline of human development (based on SmartBook
user data).
Acknowledgments
It takes an army of people to make a textbook, and while I don’t work
with everyone directly, I greatly appreciate the contributions of each per-
son who makes it possible.
Matt Taylor continues to be my right-hand man, participating in
every stage of book development; in addition, he has seamlessly inte-
grated the book’s approach into our digital assets. His hard work, exper-
tise, and eye for detail have improved every chapter in large and small
ways. In addition, Sarah Greenwood has scrutinized every illustration,
contributing a valuable student perspective to this book.
I appreciate the help of my colleagues at the University of Okla-
homa, including Dr. Doug Gaffin, Dr. Ben Holt, Dr. Heather Ketchum,
Dr. Cameron Siler, Dr. Doug Mock, and Lynn Nichols. Helpful col-
leagues from other institutions include Dr. Tamar Goulet, who has
provided insightful comments on LearnSmart prompts.
My team at McGraw-Hill is wonderful. Thank you to Managing
Director Thomas Timp and Executive Portfolio Manager Michelle
Vogler, who help us navigate the ever-changing terrain in the pub-
lishing world. Product Developer Anne Winch continues to amaze us
with her insights and sense of humor. Marketing Manager Britney
Ross and Market Development Manager Beth Theisen are skillful and
enthusiastic marketers. Emily Tietz continues to provide excellent ser-
vice in photo selections. I also appreciate Program Manager Angie
Fitzpatrick and Content Project Manager Vicki Krug for capably steer-
ing the book through production. Also among the talented folks at
McGraw-Hill are Lead Digital Product Analyst Eric Weber, Content
Licensing Specialist Lori Hancock, Designer Tara McDermott, and
Assessment Content Project Manager Christina Nelson. Thanks to all
of you for all you do.
MPS produced the art and composed the beautiful page layouts. I
appreciate their artistic talent and creative ideas for integrating the nar-
rative with the illustrations.
My family and friends continue to encourage me. Thank you to my
parents, my sister, and my in-laws for their pride and continued support. I
also thank my friends Kelly Damphousse, Ben and Angie Holt, Michael
Markham and Kristi Isacksen, Karen and Bruce Renfroe, Ingo and An-
drea Schlupp, Clarke and Robin Stroud, Matt Taylor and Elise Knowlton,
Mark Walvoord, and Michael Windelspecht. Smudge and Snorkels oc-
casionally keep me company in the office as well. Finally, my husband.
Doug Gaffin, is always there for me, helping in countless large and small
ways. I could not do this work without him.

Content Reviewers
Nicole Ashpole M. Cameron Harmon Julie Posey
University of Mississippi School of Fayetteville Technical Community College Columbus State Community College
Pharmacy Manjushri Kishore Randal Snyder
Eddie Chang Heartland Community College SUNY Buffalo State
Imperial Valley College Jocelyn Krebs Robert Stark
Ray Emmett University of Alaska, Anchorage California State University, Bakersfield
Daytona State College Catarina Mata Ellen Young
Michele Engel Borough of Manhattan Community College College of San Mateo
University of California Bakersfield

Focus Group Participants

Nancy Buschhaus Michele B. Garrett Caroline Odewumi
University of Tennessee at Martin Guilford Technical Community College Florida A&M University
Jocelyn Cash Bridgette Kirkpatrick Tanya Smutka
Central Piedmont Community College Collin College Inver Hills Community College
Matthew Cox Elizabeth A. Mays Pamela Thinesen
Central Carolina Technical College Illinois Central College Century College
Christina Fieber Reid L. Morehouse Martin Zahn
Horry Georgetown Technical College Ivy Tech Community College Thomas Nelson Community College

xvi
Detailed Contents
Brief Contents iii | About the Author iv | An Introduction for Students Using This Textbook vi | Author’s Guide to Using This Textbook vii |
McGraw-Hill Connect® x | Changes by Chapter xii | Acknowledgments xvi

UNIT 1 Science, Chemistry, and Cells

1 The Scientific Study of Life 2 2.3 Water Is Essential to Life 29

A. Water Is Cohesive and Adhesive 29
1.1 What Is Life? 3 B. Many Substances Dissolve in Water 29
A. Life Is Organized 5 C. Water Regulates Temperature 30
B. Life Requires Energy 5 D. Water Expands As It Freezes 30
C. Life Maintains Internal E. Water Participates in Life’s
Constancy 6 Chemical Reactions 31
D. Life Reproduces, Grows,
and Develops 6 2.4 Cells Have an Optimum pH 32
©IM_photo/Shutterstock RF E. Life Evolves 7 2.5 Cells Contain Four Major Types of Organic
1.2 The Tree of Life Includes Three Molecules 33
Main Branches 9 A. Large Organic Molecules Are Composed
of Smaller Subunits 33
1.3 Scientists Study the Natural World 10 B. Carbohydrates Include Simple Sugars
A. The Scientific Method Has Multiple and Polysaccharides 34
Interrelated Parts 10 C. Proteins Are Complex and Highly Versatile 36
B. An Experimental Design Is a Careful Plan 12 D. Nucleic Acids Store and Transmit
C. Theories Are Comprehensive Explanations 13 Genetic Information 38
D. Scientific Inquiry Has Limitations 14 E. Lipids Are Hydrophobic and Energy-Rich 40
E. Biology Continues to Advance 16
Why We Care 2.1 Acids and Bases in Everyday Life 34
Burning Question 1.1 Are viruses alive? 8 Burning Question 2.1 What does it mean when food is “organic”
Why We Care 1.1 It’s Hard to Know What’s Bad for You 15 or “natural”? 35
Burning Question 1.2 Why am I here? 16 Why We Care 2.2 Sugar Substitutes and Fake Fats 42
Investigating Life 1.1 The Orchid and the Moth 16 Burning Question 2.2 What is junk food? 43
Investigating Life 2.1 Chemical Warfare on a Tiny Battlefield 44

2 The Chemistry of Life 20

2.1 Atoms Make Up All Matter 21
3 Cells 48
A. Elements Are Fundamental Types 3.1 Cells Are the Units of Life 49
of Matter 21 A. Simple Lenses Revealed the First
B. Atoms Are Particles of Glimpses of Cells 49
Elements 22 B. Microscopes Magnify Cell
C. Isotopes Have Different Numbers Structures 49
of Neutrons 23 C. All Cells Have Features in
©ML Harris/Getty Images
LM (fluorescently labeled) 20 μm
Common 51
©Thomas Deerinck, NCMIR/
Science Source
2.2 Chemical Bonds Link Atoms 24
A. Electrons Determine Bonding 25 3.2 Different Cell Types Characterize Life’s Three
B. In an Ionic Bond, One Atom Transfers Electrons to Domains 52
Another Atom 25 A. Domains Bacteria and Archaea Contain Prokaryotic
C. In a Covalent Bond, Atoms Share Electrons 26 Organisms 52
D. Partial Charges on Polar Molecules Create Hydrogen B. Domain Eukarya Contains Organisms with Complex
Bonds 28 Cells 53
xvii
xviii Detailed Contents
3.3 A Membrane Separates Each Cell from Its
Surroundings 54
3.4 Eukaryotic Organelles
Divide Labor 56
A. The Nucleus, Endoplasmic Reticulum,
and Golgi Interact to Secrete Substances 57
B. Lysosomes, Vacuoles, and Peroxisomes Are Cellular
Digestion Centers 59
C. Mitochondria Extract Energy from Nutrients 60
D. Photosynthesis Occurs in Chloroplasts 60
3.5 The Cytoskeleton Supports Eukaryotic Cells 62
3.6 Cells Stick Together and Communicate with One
Another 64
Burning Question 3.1 Is it possible to make an artificial cell from
scratch? 55
Why We Care 3.1 Most of Your Cells Are Not Your Own 57
Investigating Life 3.1 Bacterial Magnets 65
4 The Energy of Life 68
4.1 All Cells Capture and Use
Energy 69
A. Energy Allows Cells to Do Life’s
Work 69
B. Life Requires Energy
Transformations 70
4.2 Networks of Chemical
©maxpro/Shutterstock RF
Reactions Sustain Life 71
A. Chemical Reactions Absorb or Release
Energy 71
B. Linked Oxidation and Reduction
Reactions Form Electron Transport Chains 71
4.3 ATP Is Cellular Energy Currency 72
A. Energy in ATP Is Critical to the
Life of a Cell 72
B. ATP Represents Short-Term Energy Storage 73
4.4 Enzymes Speed Reactions 74
A. Enzymes Bring Reactants Together 74
B. Many Factors Affect Enzyme Activity 74
4.5 Membrane Transport May Release Energy or Cost
Energy 75
A. Passive Transport Does Not Require Energy Input 76
B. Active Transport Requires Energy Input 78
C. Endocytosis and Exocytosis Use Vesicles to Transport
Substances 79
Why We Care 4.1 Enzymes Are Everywhere 74
Burning Question 4.1 Do hand sanitizers work? 75
Investigating Life 4.1 Does Natural Selection Maintain Cystic
Fibrosis? 80
5 Photosynthesis 84
5.1 Life Depends on
Photosynthesis 85
5.2 Photosynthetic Pigments Capture
Sunlight 86
5.3 Chloroplasts Are the Sites
of Photosynthesis 87
© Rodrigo A. Torres/
Glowimages
5.4 Photosynthesis Occurs in Two Stages 88
5.5 The Light Reactions Begin Photosynthesis 89
A. Light Striking Photosystem II Provides
the Energy to Produce ATP 90
B. Electrons from Photosystem I Reduce NADP+ to NADPH 91
5.6 The Carbon Reactions Produce Carbohydrates 92
5.7 C3, C4, and CAM Plants Use Different Carbon Fixation
Pathways 93
Burning Question 5.1 Why do leaves change colors in the fall? 89
Burning Question 5.2 Does air have mass? 91
Why We Care 5.1 Weed Killers 93
Investigating Life 5.1 Salamanders Snack on Sugars from Solar
Cells 95
6 Respiration and Fermentation 98
6.1 Cells Use Energy in Food to Make
ATP 99
6.2 Cellular Respiration Includes
Three Main Processes 100
6.3 In Eukaryotic Cells, Mitochondria
Produce Most ATP 101
©Three Images/Lifesize/
Getty Images RF
6.4 Glycolysis Breaks Down Glucose to Pyruvate 102
6.5 Aerobic Respiration Yields Much More ATP than
Glycolysis Alone 103
A. Pyruvate Is Oxidized to Acetyl CoA 103
B. The Krebs Cycle Produces ATP and High-Energy Electron
Carriers 104
C. The Electron Transport Chain Drives ATP Formation 105
6.6 How Many ATPs Can One Glucose Molecule Yield? 106
6.7 Other Food Molecules Enter the Energy-Extracting
Pathways 107
6.8 Fermenters Acquire ATP Only in Glycolysis 108
Why We Care 6.1 Some Poisons Inhibit Respiration 103
Burning Question 6.1 How do diet pills work? 105
Burning Question 6.2 What happens during hibernation? 107
Investigating Life 6.1 Hot Plants Offer Heat Rewards 109
 Detailed Contents xix

UNIT 2 DNA, Inheritance, and Biotechnology

7 DNA Structure and Gene 8 DNA Replication, Binary Fission,

Function 112
7.1 DNA Is a Double Helix 113
7.2 DNA Stores Genetic Information:
An Overview 115
7.3 Transcription Uses a DNA
Template to Build RNA 116
©PhotoAlto/Getty
©G. Murti/Science Source
TEM (false color)
1 μm
and Mitosis 138
8.1 Cells Divide and Cells Die 139
A. Sexual Life Cycles Include
Mitosis, Meiosis, and
Fertilization 139
B. Cell Death Is Part of Life 140
8.2 DNA Replication Precedes
Cell Division 141

Images RF

7.4 Translation Builds the Protein 118 8.3 Bacteria and Archaea Divide by Binary Fission 142
A. Translation Requires mRNA, tRNA,
and Ribosomes 118 8.4 Replicated Chromosomes Condense as a Eukaryotic
B. Translation Occurs in Three Steps 119 Cell Prepares to Divide 143
C. Proteins Must Fold Correctly after
8.5 Mitotic Division Generates Exact Cell Copies 145
Translation 120
A. DNA Is Copied During Interphase 145
7.5 Cells Regulate Gene Expression 121 B. Chromosomes Divide During Mitosis 146
A. Operons Are Groups of Bacterial Genes That Share One C. The Cytoplasm Splits in Cytokinesis 148
Promoter 121
8.6 Cancer Cells Divide Uncontrollably 148
B. Eukaryotic Organisms Use Many
A. Chemical Signals Regulate Cell Division 148
Regulatory Methods 121
B. Cancer Cells Are Malignant 149
7.6 Mutations Change DNA 123 C. Cancer Treatments Remove or Kill Abnormal Cells 149
A. Mutations Range from Silent to D. Genes and Environment Both Can
Devastating 123 Increase Cancer Risk 150
B. What Causes Mutations? 124 Burning Question 8.1 Do all human cells divide at the same
C. Mutations Are Important for Many Reasons 125 rate? 145
Why We Care 8.1 Skin Cancer 150
7.7 Viruses Are Genes Wrapped in a Protein
Investigating Life 8.1 Evolutionary Strategies in the Race Against
Coat 126 Cancer 150
A. Viruses Are Smaller and Simpler than Cells 126
B. Viral Replication Occurs in Five Stages 127

7.8 Viruses Infect All Cell Types 128 9 Sexual Reproduction and Meiosis 154
A. Bacteriophages May Kill Cells Immediately
9.1 Why Sex? 155
or “Hide” in a Cell 128
B. Animal Viruses May Cause Immediate 9.2 Diploid Cells Contain
Cell Death 129 Two Homologous Sets of
C. Some Animal Viruses Linger for Years 129 Chromosomes 156
D. Viruses Cause Diseases in Plants 131
9.3 Meiosis Is Essential
7.9 Drugs and Vaccines Help Fight Viral in Sexual Reproduction 157
Infections 131 ©IT Stock/age fotostock RF

7.10 Viroids and Prions Are Other Noncellular
Infectious Agents 132
Why We Care 7.1 Poisons That Block Protein Production 120
Burning Question 7.1 Is there a gay gene? 125
Burning Question 7.2 Why do we get sick when the weather turns
cold? 128
Investigating Life 7.1 Clues to the Origin of Language 133
9.4 In Meiosis, DNA Replicates Once, but the Nucleus
Divides Twice 158
9.5 Meiosis Generates Enormous Variability 160
A. Crossing Over Shuffles Alleles 160
B. Homologous Pairs Are Oriented Randomly During
Metaphase I 161
C. Random Fertilization Multiplies the Diversity 162
xx Detailed Contents

9.6 Mitosis and Meiosis Have Different Functions: A
Summary 162
9.7 Errors Sometimes Occur in Meiosis 164
A. Polyploidy Means Extra Chromosome Sets 164
B. Nondisjunction Results in Extra
or Missing Chromosomes 164
Burning Question 9.1 If mules are sterile, then
how are they produced? 160
Why We Care 9.1 Multiple Births 164
Investigating Life 9.1 Evolving Germs Select for Sex in
Worms 167
10 Patterns of Inheritance 170
B. X Inactivation Prevents “Double Dosing”
of Proteins 184
10.8 Pedigrees Show Modes of Inheritance 186
10.9 Most Traits Are Influenced by the Environment and
Multiple Genes 188
A. The Environment Can Alter the Phenotype 188
B. Polygenic Traits Depend on More than One Gene 189
Burning Question 10.1 Why does diet soda have
a warning label? 174
Burning Question 10.2 Is male baldness really from the female side
of the family? 186
Why We Care 10.1 The Origin of Obesity 188
Investigating Life 10.1 Heredity and the Hungry Hordes 190

10.1 Chromosomes Are Packets

of Genetic Information: A
Review 171 11 DNA Technology 196
10.2 Mendel’s Experiments 11.1 DNA Technology Is Changing
Uncovered Basic Laws of the World 197
Inheritance 172
11.2 DNA Technology’s Tools Apply
A. Dominant Alleles Appear to Mask
©Rick Gomez/Corbis/Getty to Individual Genes or Entire
Recessive Alleles 173
Images Genomes 198
B. For Each Gene, a Cell’s Two Alleles May Be A. Transgenic Organisms
Identical or Different 174 ©Tony Gutierrez/AP Images Contain DNA from Multiple
Sources 198
10.3 The Two Alleles of a Gene End Up in Different
Gametes 175 B. DNA Sequencing Reveals the Order of Bases 200
A. The Simplest Punnett Squares Track the Inheritance of C. PCR Replicates DNA in a Test Tube 202
One Gene 175 D. DNA Profiling Detects Genetic Differences 202
B. Meiosis Explains Mendel’s Law of Segregation 176
11.3 Stem Cells and Cloning Add New Ways to Copy Cells
10.4 Genes on Different Chromosomes Are Inherited and Organisms 205
Independently 178 A. Stem Cells Divide to Form Multiple
A. Tracking Two-Gene Inheritance May Require Large Cell Types 205
Punnett Squares 178 B. Cloning Produces Identical Copies
B. Meiosis Explains Mendel’s Law of an Organism 206
of Independent Assortment 178
11.4 Many Medical Tests and Procedures Use DNA
C. The Product Rule Is a Useful Shortcut 178
Technology 208
10.5 Genes on the Same Chromosome May Be Inherited A. DNA Probes Detect Specific Sequences 208
Together 180 B. Preimplantation Genetic Diagnosis Can Screen Embryos
A. Genes on the Same Chromosome Are Linked 180 for Some Diseases 208
B. Studies of Linked Genes Have Yielded Chromosome C. Genetic Testing Can Detect Existing Diseases 209
Maps 181 D. Gene Therapy Uses DNA to Treat Disease 209
E. CRISPR-Cas9 Cuts and Edits Specific Genes 210
10.6 Inheritance Patterns Are Rarely Simple 182 F. Medical Uses of DNA Technology
A. Incomplete Dominance and Codominance Add Phenotype Raise Many Ethical Issues 211
Classes 182
B. Relating Genotype to Phenotype May Be Difficult 182 Burning Question 11.1 Is selective breeding the same as genetic
engineering? 198
10.7 Sex-Linked Genes Have Unique Inheritance Burning Question 11.2 What are the uses of DNA testing? 204
Patterns 184 Why We Care 11.1 Gene Doping 211
A. X-Linked Recessive Disorders Affect More Males than Investigating Life 11.1 Weeds Get a Boost from Their Transgenic
Females 184 Cousins 212
 Detailed Contents xxi

UNIT 3 Evolution and Diversity

12 Forces of Evolutionary Change 216

13.3 Biogeography Considers Species’ Geographical
Locations 243
12.1 Evolution Acts on A. The Theory of Plate Tectonics
Populations 217 Explains Earth’s Shifting Continents 243
B. Species Distributions Reveal Evolutionary Events 244
12.2 Evolutionary Thought Has
Evolved for Centuries 218 13.4 Anatomical Comparisons May Reveal Common
A. Many Explanations Have Been Descent 245
Proposed for Life’s Diversity 218 A. Homologous Structures Have
B. Charles Darwin’s Voyage Provided a Shared Evolutionary Origin 245
©Steven Hunt/Stone/Getty
a Wealth of Evidence 219 B. Vestigial Structures Have Lost Their Functions 245
Images C. Convergent Evolution Produces Superficial
C. On the Origin of Species Proposed Natural Selection as an Similarities 246
Evolutionary Mechanism 220
13.5 Embryonic Development Patterns Provide Evolutionary
D. Evolutionary Theory Continues to Expand 222
Clues 246
12.3 Natural Selection Molds Evolution 223
A. Adaptations Enhance Reproductive Success 223 13.6 Molecules Reveal Relatedness 248
B. Natural Selection Eliminates Poorly Adapted Phenotypes 224 A. Comparing DNA and Protein Sequences
C. Natural Selection Does Not Have a Goal 224 May Reveal Close Relationships 248
D. What Does “Survival of the Fittest” Mean? 225 B. Molecular Clocks Help Assign
Dates to Evolutionary Events 249
12.4 Evolution Is Inevitable in Real Populations 226
Burning Question 13.1 Does the fossil record include transitional
A. At Hardy–Weinberg Equilibrium, Allele Frequencies Do
forms? 241
Not Change 226
Why We Care 13.1 An Evolutionary View of the Hiccups 248
B. In Reality, Allele Frequencies Always Change 227
Investigating Life 13.1 Evolving Backwards 250
12.5 Natural Selection Can Shape Populations in
Many Ways 228
12.6 Sexual Selection Directly Influences Reproductive
14 Speciation and Extinction 254
Success 230 14.1 What Is a Species? 255
A. Linnaeus Classified Life Based on
12.7 Evolution Occurs in Several Additional Ways 231 Appearance 255
A. Mutation Fuels Evolution 231 B. Species Can Be Defined Based on
B. Genetic Drift Occurs by Chance 231 the Potential to Interbreed 255
C. Nonrandom Mating Concentrates Alleles Locally 233
D. Migration Moves Alleles Between Populations 233 14.2 Reproductive Barriers Cause
Species to Diverge 256
Why We Care 12.1 Dogs Are Products of Artificial Selection 220 ©Kike Calvo/National
Geographic/Getty Images
A. Prezygotic Barriers Prevent
Burning Question 12.1 Is there such a thing as a “pinnacle of
Fertilization 258
evolution”? 226
B. Postzygotic Barriers Prevent Development
Why We Care 12.2 The Unending War with Bacteria 229
of a Fertile Offspring 258
Investigating Life 12.1 Bacterial Evolution Goes “Hog Wild” on the
Farm 234 14.3 Spatial Patterns Define Two Types of Speciation 259
A. Allopatric Speciation Reflects a Geographical Barrier 259
13 Evidence of Evolution 238 B. Sympatric Speciation Occurs in a Shared Habitat 260
C. Determining the Type of Speciation May Be Difficult 261
13.1 Clues to Evolution Lie in the
Earth, Body Structures, and 14.4 Speciation May Be Gradual or May Occur in Bursts 262
Molecules 239 14.5 Extinction Marks the End of the Line 263
13.2 Fossils Record Evolution 241 14.6 Biological Classification Systems
A. The Fossil Record Is Often Are Based on Common Descent 265
Incomplete 241 A. The Taxonomic Hierarchy Organizes Species into Groups 265
©Martin Shields/
B. The Age of a Fossil Can Be B. A Cladistics Approach Is Based on Shared Derived
Alamy Stock Photo Estimated in Two Ways 242 Traits 265
xxii Detailed Contents

C. Cladograms Depict Hypothesized Burning Question 15.3 Why and how do algae form? 285
Evolutionary Relationships 266 Why We Care 15.2 Preventing Mold 296
D. Many Traditional Groups Are Not Clades 267 Investigating Life 15.1 Shining a Spotlight on Danger 297
Burning Question 14.1 Can people watch evolution and speciation in
action? 258
Why We Care 14.1 Recent Species Extinctions 264 16 Evolution and Diversity of Plants 300
Burning Question 14.2 Did rabbits come from frogs? 267 16.1 Plants Have Changed the
Investigating Life 14.1 Plant Protection Rackets May Stimulate World 301
Speciation 268
A. Green Algae Are the Closest
Relatives of Plants 301
B. Plants Are Adapted to Life on
15 Evolution and Diversity Land 303
of Microbial Life 272 16.2 Bryophytes Are the
©Joe Scherschel/National
15.1 Life’s Origin Remains Geographic/Getty Images Simplest Plants 306
Mysterious 273
16.3 Seedless Vascular Plants Have Xylem and Phloem
A. The First Organic Molecules
but No Seeds 308
May Have Formed in a Chemical
“Soup” 274 16.4 Gymnosperms Are “Naked Seed” Plants 310
B. Clays May Have Helped
Monomers Form Polymers 275 16.5 Angiosperms Produce Seeds in Fruits 312
©Europics/Newscom Burning Question 16.1 Do all plants live on land? 303
C. Membranes Enclosed the Molecules 276 Burning Question 16.2 What are biofuels? 304
D. Early Life Changed Earth Forever 276 Why We Care 16.1 Gluten and Human Health 313
Investigating Life 16.1 Genetic Messages from Ancient Ecosystems 314
15.2 Prokaryotes Are a Biological Success Story 277
A. What Is a Prokaryote? 277
B. Prokaryote Classification Traditionally Relies on Cell
Structure and Metabolism 278
17 Evolution and Diversity
C. Prokaryotes Include Two Domains with Enormous of Animals 318
Diversity 280 17.1 Animals Live Nearly
D. Bacteria and Archaea Are Essential to All Life 281 Everywhere 319
15.3 Eukaryotic Cells and Multicellularity Arose More A. What Is an Animal? 319
Than a Billion Years Ago 284 B. Animal Life Began in the
A. Endosymbiosis Explains the Origin of Mitochondria and Water 319
Chloroplasts 284 C. Animal Features Reflect Shared
B. Multicellularity May Also Ancestry 320
Have Its Origin in Cooperation 286 ©Imagemore Co, Ltd./ D. Biologists Also Consider
Getty Images RF Additional Characteristics 322
15.4 Protists Are the Simplest Eukaryotes 287 17.2 Sponges Are Simple Animals That Lack Differentiated
A. What Is a Protist? 287 Tissues 323
B. Algae Are Photosynthetic Protists 287
C. Some Heterotrophic Protists Were Once Classified as 17.3 Cnidarians Are Radially Symmetrical, Aquatic
Fungi 289 Animals 324
D. Protozoa Are Diverse Heterotrophic Protists 290
17.4 Flatworms Have Bilateral Symmetry and Incomplete
15.5 Fungi Are Essential Decomposers 292 Digestive Tracts 325
A. What Is a Fungus? 292
17.5 Mollusks Are Soft, Unsegmented Animals 326
B. Fungal Classification Is Based
on Reproductive Structures 293 17.6 Annelids Are Segmented Worms 327
C. Fungi Interact with Other Organisms 294
17.7 Nematodes Are Unsegmented, Cylindrical Worms 328
Burning Question 15.1 Does new life spring from simple molecules
now, as it did in the past? 276 17.8 Arthropods Have Exoskeletons and Jointed
Why We Care 15.1 Antibiotics and Other Germ Killers 282 Appendages 329
Burning Question 15.2 Are there areas on Earth where no life A. Arthropods Have Complex Organ Systems 329
exists? 284 B. Arthropods Are the Most Diverse Animals 330
 Detailed Contents xxiii

17.9 Echinoderm Adults Have Five-Part, Radial Symmetry 334
17.10 Most Chordates Are Vertebrates 335
17.11 Chordate Diversity Extends from Water to Land to Sky 337
A. Tunicates and Lancelets Are Invertebrate Chordates 337
B. Hagfishes and Lampreys Have a Cranium but Lack Jaws 338
C. Fishes Are Aquatic Vertebrates with Jaws, Gills, and
Fins 338
D. Amphibians Live on Land and in Water 340
E. Reptiles Were the First Vertebrates to Thrive on Dry
Land 340
F. Mammals Are Warm, Furry Milk-Drinkers 342
17.12 Fossils and DNA Tell the Human Evolution Story 343
A. Humans Are Primates 343
B. Anatomical and Molecular Evidence Documents Primate
Relationships 344
C. Human Evolution Is Partially Recorded in Fossils 345
D. Environmental Changes Have Spurred
Human Evolution 347
E. Migration and Culture Have Changed Homo sapiens 347
Burning Question 17.1 Are there really only nine kinds of animals? 331
Why We Care 17.1 Your Tiny Companions 333
Burning Question 17.2 Did humans and dinosaurs ever coexist? 347
Investigating Life 17.1 Discovering the “Fishapod” 349

UNIT 4 Ecology

18 Populations 356
A. The Physical Environment Dictates Where Each Species
Can Live 376
18.1 Ecology Is the Study of B. Terrestrial Biomes Range from the Lush Tropics to the
Interactions 357 Frozen Poles 377
C. Aquatic Biomes Include Fresh Water and the Oceans 381
18.2 A Population’s Size and Density
Change Over Time 358 19.4 Community Interactions Occur Within Each
Biome 381
18.3 Births and Deaths Help A. Many Species Compete for the Same Resources 382
Determine Population Size 359 B. Symbiotic Interactions Can Benefit or Harm a Species 383
©Donal Husni/NurPhoto via C. Herbivory and Predation Link Species in
Getty Images
Feeding Relationships 383
18.4 Natural Selection Influences Life Histories 361 D. Closely Interacting Species May Coevolve 384
A. Organisms Balance Reproduction Against E. A Keystone Species Has a Pivotal Role in
Other Requirements 361 the Community 385
B. Opportunistic and Equilibrium Life Histories Reflect the
Trade-Off Between Quantity and Quality 362 19.5 Succession Is a Gradual Change in a Community 386

18.5 Population Growth May Be Exponential or Logistic 363
A. Growth Is Exponential When
Resources Are Unlimited 363
B. Population Growth Eventually Slows 364
C. Many Conditions Limit Population Size 364
18.6 The Human Population Continues to Grow 366
A. Birth and Death Rates Vary Worldwide 366
B. The Ecological Footprint Is an Estimate of Resource Use 368
Burning Question 18.1 How do biologists count animals in the
open ocean? 361
Why We Care 18.1 Controlling Animal Pests 365
Investigating Life 18.1 A Toxic Compromise 369
19.6 Ecosystems Require Continuous Energy Input 388
A. Food Webs Depict the Transfer of Energy and Atoms 388
B. Heat Energy Leaves Each Food Web 390
C. Harmful Chemicals May Accumulate
in the Highest Trophic Levels 391
19.7 Chemicals Cycle Within Ecosystems 392
A. Water Circulates Between the
Land and the Atmosphere 393
B. Autotrophs Obtain Carbon as CO2 394
C. The Nitrogen Cycle Relies on Bacteria 395
D. The Phosphorus Cycle Begins with the Weathering of
Rocks 397
E. Excess Nitrogen and Phosphorus Cause Problems in

19 Communities and Ecosystems 372

19.1 Organisms Interact
Within Communities and
Ecosystems 373
19.2 Earth Has Diverse Climates 374
19.3 Biomes Are Ecosystems with
Distinctive Communities of
©Pedro Ladeira/SambaPhoto/ Life 376
Getty Images
Water 397
Burning Question 19.1 Why is there a “tree line” above which trees
won’t grow? 377
Why We Care 19.1 What Happens After You Flush 388
Why We Care 19.2 Mercury on the Wing 390
Burning Question 19.2 Is bottled water safer than tap water? 393
Why We Care 19.3 The Nitrogen Cycle in Your Fish Tank 396
Investigating Life 19.1 Winged Migrants Sidestep Parasites 398
xxiv Detailed Contents
20 Preserving Biodiversity 402
20.1 Earth’s Biodiversity Is
Dwindling 403
20.2 Many Human Activities Destroy
Habitats 404
20.3 Pollution Degrades Habitats 406
A. Water Pollution Threatens
Source: CINMS/NOAA/Claire Fackler Aquatic Life 406
B. Air Pollution Causes Many Types of Damage 407
20.4 Global Climate Change Alters and Shifts Habitats 409
A. Greenhouse Gases Warm Earth’s Surface   409
B. Global Climate Change Has Severe Consequences   411
20.5 Exotic Invaders and Overexploitation Devastate
Many Species 412
A. Invasive Species Displace Native Organisms 412
B. Overexploitation Can Drive Species to Extinction 413
UNIT 5 Plant Anatomy and Physiology
21 Plant Form and Function 420
21.1 Vegetative Plant Parts Include
Stems, Leaves, and Roots 421
21.2 Soil and Air Provide Water
and Nutrients 422
A. Plants Require 16 Essential
Elements 422
©Bob Gibbons/Alamy Stock
B. Leaves and Roots Absorb
Photo Essential Elements 423
21.3 Plant Cells Build Tissues 424
A. Plants Have Several Cell Types 424
B. Plant Cells Form Three Main Tissue Systems 426
21.4 Tissues Build Stems, Leaves, and Roots 427
A. Stems Support Leaves 427
B. Leaves Are the Primary Organs of Photosynthesis 427
C. Roots Absorb Water and Minerals, and Anchor the
Plant 429
21.5 Plants Have Flexible Growth
Patterns, Thanks to Meristems 430
A. Plants Grow by Adding New Modules 430
B. Plant Growth Occurs at Meristems 431
C. In Primary Growth, Apical Meristems Lengthen Stems
and Roots 431
D. In Secondary Growth, Lateral Meristems Thicken Stems
and Roots 432
21.6 Vascular Tissue Transports Water, Minerals, and
Sugar 434
20.6 Some Biodiversity May Be Recoverable 414
A. Protecting and Restoring Habitat Saves Many Species at
Once 414
B. Some Conservation Tools Target Individual Species 414
C. Conserving Biodiversity Involves Scientists
and Ordinary Citizens 415
Burning Question 20.1 What are the best ways to reverse habitat
destruction? 406
Burning Question 20.2 How can people reduce their contribution to
water pollution? 408
Burning Question 20.3 What does the ozone hole have to do with
global climate change? 409
Burning Question 20.4 How can small lifestyle changes reduce air
pollution and global climate change? 411
Burning Question 20.5 How can people help slow the spread of
invasive species? 412
Burning Question 20.6 Can everyday buying decisions help protect
overharvested species? 413
Why We Care 20.1 Environmental Legislation 415
Investigating Life 20.1 Up, Up, and Away 416
A. Water and Minerals Are Pulled Up to Leaves in Xylem 434
B. Sugars Are Pushed to Nonphotosynthetic Cells in
Phloem 436
C. Parasitic Plants Tap into Another Plant’s Vascular Tissue 437
Burning Question 21.1 What’s the difference between fruits and
vegetables? 422
Why We Care 21.1 Boost Plant Growth with Fertilizer 424
Burning Question 21.2 What are controlled burns? 433
Burning Question 21.3 Where does maple syrup come from? 437
Investigating Life 21.1 An Army of Tiny Watchdogs 438
22 Reproduction and Development of
Flowering Plants 442
22.1 Angiosperms Reproduce Sexually
and Asexually 443
22.2 The Angiosperm Life Cycle
Includes Flowers, Fruits, and
Seeds 444
A. Flowers Are Reproductive
Organs 445
©Gay Bumgarner/Alamy
Stock Photo B. The Pollen Grain and Embryo Sac
Are Gametophytes 445
C. Pollination Brings Pollen to the Stigma 446
D. Double Fertilization Yields Zygote and Endosperm 446
E. A Seed Is an Embryo and Its Food Supply Inside a Seed
Coat 447
F. The Fruit Develops from the Ovary 448
G. Fruits Protect and Disperse Seeds 449
 Detailed Contents xxv

22.3 Plant Growth Begins with Seed Germination 450
22.4 Hormones Regulate Plant
Growth and Development 451
A. Auxins and Cytokinins Are Essential for Plant Growth 452
B. Gibberellins, Ethylene, and Abscisic Acid Influence Plant
Development in Many Ways 453
22.5 Light Is a Powerful Influence on Plant Life 454
22.6 Plants Respond to Gravity and Touch 455
Burning Question 22.1 How can a fruit be seedless? 451
Why We Care 22.1 Talking Plants 452
Investigating Life 22.1 A Red Hot Chili Pepper Paradox 456

UNIT 6 Animal Anatomy and Physiology

B. The Nervous System Includes Three
23 Animal Tissues and Organ Classes of Neurons 478
Systems 460 24.3 Action Potentials Convey Messages 479
23.1 S
 pecialized Cells Build A. A Neuron at Rest Has a Negative Charge 480
Animal Bodies 461 B. A Neuron’s Membrane Potential Reverses During an
Action Potential 480
23.2 Animals Consist of Four C. The Myelin Sheath Speeds Communication 482
Tissue Types 462
A. Epithelial Tissue Covers 24.4 Neurotransmitters Pass the Message from Cell to
Surfaces 462 Cell 482
©James King-Holmes/Science B. Most Connective 24.5 The Peripheral Nervous System Consists
Source Tissues Bind Other of Nerve Cells Outside the Central Nervous
Tissues Together 464 System 484
C. Muscle Tissue Provides Movement 464
D. Nervous Tissue Forms a Rapid 24.6 The Central Nervous System Consists of the Spinal
Communication Network 465 Cord and Brain 486
23.3 Organ Systems Are Interconnected 466 A. The Spinal Cord Transmits Information Between Body
A. The Nervous and Endocrine Systems and Brain 486
Coordinate Communication 466 B. The Brain Is Divided into Several Regions 486
B. The Skeletal and Muscular Systems Support C. Many Brain Regions Participate in Memory 488
and Move the Body 466 D. Damage to the Central Nervous System
C. The Digestive, Circulatory, and Respiratory Systems Can Be Devastating 489
Work Together to Acquire Energy 467 24.7 The Senses Connect the Nervous System with the
D. The Urinary, Integumentary, Immune, and Lymphatic Outside World 490
Systems Protect the Body 467 A. Sensory Receptors Respond to Stimuli by Generating
E. The Reproductive System Produces the Next Generation 468 Action Potentials 490
23.4 Organ System Interactions Promote Homeostasis 468 B. Continuous Stimulation May Cause Sensory
23.5 Animals Regulate Body Temperature 469 Adaptation 491
Why We Care 23.1 Two Faces of Plastic Surgery 465 24.8 The General Senses Detect Touch, Temperature,
Burning Question 23.1 How does the body react to food poisoning? 466 and Pain 491
Burning Question 23.2 Can biologists build artificial organs? 471
24.9 The Senses of Smell and Taste Detect
Investigating Life 23.1 Sniffing Out the Origin of Feathers 472
Chemicals 492

24 The Nervous System and the 24.10 Vision Depends on Light-Sensitive Cells 494

Senses 476 24.11 The Sense of Hearing Begins in the Ears 496

24.1 The Nervous System Forms a
Rapid Communication
Network 477
24.2 Neurons Are the Functional Units
of a Nervous System 478
A. A Typical Neuron Consists of
a Cell Body, Dendrites, and an
©Cary Wolinsky/Getty Images
Axon 478
Burning Question 24.1 Do neurons communicate at the speed of
light? 480
Why We Care 24.1 Drugs and Neurotransmitters 484
Burning Question 24.2 Do I really use only 10% of my brain? 488
Burning Question 24.3 Do humans have pheromones? 493
Why We Care 24.2 Correcting Vision 495
Burning Question 24.4 What is an ear infection? 496
Investigating Life 24.1 Scorpion Stings Don’t Faze Grasshopper
Mice 498
xxvi Detailed Contents

25 The Endocrine System 502

25.1 The Endocrine System Uses
Hormones to Communicate 503
25.2 Hormones Stimulate Responses
in Target Cells 504
A. Water-Soluble Hormones Trigger
Second Messenger Systems 504
©Purestock/SuperStock RF
B. Lipid-Soluble Hormones Directly
Alter Gene Expression 505
25.3 The Hypothalamus and Pituitary
Gland Oversee Endocrine Control 505
A. The Posterior Pituitary Stores and Releases Two
Hormones 507
B. The Anterior Pituitary Produces and Secretes Six
Hormones 507
25.4 Hormones from Many Glands Regulate Metabolism 508
A. The Thyroid Gland Sets the Metabolic Pace 508
B. The Parathyroid Glands Control Calcium Level 509
C. The Adrenal Glands Coordinate the Body’s
Stress Responses 509
D. The Pancreas Regulates Blood Glucose 510
E. The Pineal Gland Secretes Melatonin 511
25.5 Hormones from the Ovaries and Testes Control
Reproduction 512
Burning Question 25.1 What are endocrine disruptors? 508
Why We Care 25.1 Anabolic Steroids in Sports 512
Investigating Life 25.1 Addicted to Affection 513
26 The Skeletal and Muscular
Systems 516
26.1 Skeletons Take Many Forms 517
26.2 The Vertebrate Skeleton Features
a Central Backbone 518
26.3 Bones Provide Support,
Protect Internal Organs,
and Supply Calcium 519
©Jeff J Mitchell/Getty Images
A. Bones Consist Mostly of Bone Tissue and Cartilage 519
B. Bone Meets Bone at a Joint 521
C. Bones Are Constantly Built and Degraded 521
D. Bones Help Regulate Calcium Homeostasis 522
26.4 Muscle Movement Requires Contractile Proteins and
ATP 522
A. Actin and Myosin Filaments Fill Muscle Cells 524
B. Sliding Filaments Are the Basis of Muscle
Cell Contraction 524
C. Motor Neurons Stimulate Muscle Contraction 526
26.5 Muscle Cells Generate ATP in Multiple Ways 527
26.6 Muscle Fiber Types Influence Athletic Performance 528
Why We Care 26.1 Bony Evidence of Murder, Illness, and Evolution 519
Burning Question 26.1 Is creatine a useful dietary supplement? 527
Burning Question 26.2 Why does heat soothe sore muscles and
joints? 529
Investigating Life 26.1 Did a Myosin Gene Mutation Make Humans
Brainier? 530
27 The Circulatory and Respiratory
Systems 534
27.1 Blood Plays a Central Role in
Maintaining Homeostasis 535
A. Plasma Carries Many Dissolved
Substances 536
B. Red Blood Cells Transport
Oxygen 536
C. White Blood Cells Fight
©Philippe Plailly/Science Source Infection 536
D. Blood Clotting Requires Platelets and Plasma Proteins 537
27.2 Animal Circulatory Systems Range from Simple to
Complex 538
27.3 Blood Circulates Through the Heart and Blood
Vessels 539
27.4 The Human Heart Is a Muscular Pump 540
A. The Heart Has Four Chambers 540
B. The Right and Left Halves
of the Heart Deliver Blood Along Different Paths 540
C. Cardiac Muscle Cells Produce the Heartbeat 541
D. Exercise Strengthens the Heart 542
27.5 Blood Vessels Form the Circulation Pathway 542
A. Arteries, Capillaries, and Veins
Have Different Structures 543
B. Blood Pressure and Velocity Differ Among Vessel Types 544
27.6 The Human Respiratory System Delivers Air to the
Lungs 545
A. The Nose, Pharynx, and Larynx Form the Upper
Respiratory Tract 546
B. The Lower Respiratory Tract Consists
of the Trachea and Lungs 548
27.7 Breathing Requires Pressure Changes in the Lungs 550
27.8 Red Blood Cells Carry Most Oxygen and Carbon
Dioxide 551
Burning Question 27.1 What is the difference between donating
whole blood and donating plasma? 537
Burning Question 27.2 What causes bruises? 539
Burning Question 27.3 If some exercise is good, is more exercise
better? 542
Why We Care 27.1 Unhealthy Circulatory and Respiratory
Systems 549
Investigating Life 27.1 In (Extremely) Cold Blood 552
 Detailed Contents xxvii

28 The Digestive and Urinary 29 The Immune System 580

Systems 556
28.1 Animals Maintain Nutrient, Water,
and Ion Balance 557
28.2 Digestive Systems Derive
Energy and Raw Materials from
Food 558
Source: CDC/James Gathany
28.3 A Varied Diet Is Essential
©Ingram Publishing RF to Good Health 559
28.4 Body Weight Reflects Food Intake and Activity
Level 560
A. Body Mass Index Can Identify Weight Problems 560
B. Starvation: Too Few Calories to Meet the Body’s
Needs 561
C. Obesity: More Calories Than the Body Needs 561
28.5 Most Animals Have a Specialized Digestive
Tract 562
A. Acquiring Nutrients Requires Several Steps 562
B. Digestive Tracts May Be Incomplete or Complete 563
C. Diet Influences Digestive Tract Structure 564
28.6 The Human Digestive System Consists of Several
Organs 565
A. Muscles Underlie the Digestive Tract 566
B. Digestion Begins in the Mouth 566
C. The Stomach Stores, Digests, and
Churns Food 566
D. The Small Intestine Digests and Absorbs
Nutrients 567
E. The Large Intestine Completes Nutrient and Water
Absorption 569
28.7 Animals Eliminate Nitrogenous Wastes and Regulate
Water and Ions 570
29.1 Many Cells, Tissues, and Organs
Defend the Body 581
A. White Blood Cells Play Major
Roles in the Immune System 581
B. The Lymphatic System Produces
and Transports Many Immune
System Cells 582
C. The Immune System Has Two
Main Subdivisions 582
29.2 Innate Defenses Are Nonspecific and Act Early 583
A. External Barriers Form the First Line of Defense 583
B. Internal Innate Defenses Destroy Invaders 584
29.3 Adaptive Immunity Defends Against Specific
Pathogens 586
A. Helper T Cells Play a Central Role in Adaptive
Immunity 586
B. Cytotoxic T Cells Provide Cell-Mediated Immunity 587
C. B Cells Direct the Humoral Immune Response 588
D. The Secondary Immune Response Is Stronger Than the
Primary Response 590
29.4 Vaccines Jump-Start Immunity 590
29.5 Several Disorders Affect the Immune System 592
A. Autoimmune Disorders Are Devastating
and Mysterious 592
B. Immunodeficiencies Lead to Opportunistic
Infections 592
C. Allergies Misdirect the Immune Response 593
Why We Care 29.1 Severe Burns 584
Why We Care 29.2 Protecting a Fetus from Immune Attack 587
Burning Question 29.1 Why do we need multiple doses of some
vaccines? 591
Burning Question 29.2 Can people be allergic to meat? 592
Investigating Life 29.1 The Hidden Cost of Hygiene 594

28.8 The Urinary System Produces, Stores, and Eliminates

Urine 572
30 Animal Reproduction and
28.9 Nephrons Remove Wastes and Adjust the Composition Development 598
of Blood 573
A. Nephrons Interact Closely with Blood Vessels 573 30.1 Animal Development Begins
B. Urine Formation Includes Filtration, Reabsorption, and with Reproduction 599
Secretion 574 A. Reproduction Is Asexual or
C. Hormones Regulate Kidney Function 574 Sexual 599
B. Development Is Indirect or
Burning Question 28.1 Which diets lead to the most weight loss? 561 Direct 600
Burning Question 28.2 What is lactose intolerance? 568
Why We Care 28.1 The Unhealthy Digestive System 570 ©UIG via Getty Images
Why We Care 28.2 Urinary Incontinence 572
Burning Question 28.3 What can urine reveal about health and 30.2 Males Produce Sperm Cells 601
diet? 573 A. Male Reproductive Organs Are Inside and Outside
Why We Care 28.3 Kidney Failure, Dialysis, and Transplants 575 the Body 601
Investigating Life 28.1 The Cost of a Sweet Tooth 576 B. Spermatogenesis Yields Sperm Cells 602
C. Hormones Influence Male Reproductive Function 603
xxviii Detailed Contents

30.3 Females Produce Egg Cells 604 Appendix A Answers to Multiple Choice Questions A-1
A. Female Reproductive Organs Are Inside the Body 604 Appendix B Brief Guide to Statistical Significance A-2
B. Oogenesis Yields Egg Cells 605
Appendix C Units of Measure A-5
C. Hormones Influence Female Reproductive Function 606
D. Hormonal Fluctuations Can Cause Discomfort 607 Appendix D Periodic Table of the Elements A-6
Appendix E Amino Acid Structures A-7
30.4 Reproductive Health Considers Contraception and
Disease 608 Appendix F Learn How to Learn A-8

30.5 The Human Infant Begins Life as a Zygote 611 Glossary G-1
A. Fertilization Initiates Pregnancy 611 Index I-1
B. The Preembryonic Stage Ends
When Implantation Is Complete 612
C. Organs Take Shape During the Embryonic Stage 614
D. Organ Systems Become Functional in the Fetal Stage 615
E. Muscle Contractions in the Uterus Drive Childbirth 616
Burning Question 30.1 When can conception occur? 608
Why We Care 30.1 Substances That Cause Birth Defects 613
Investigating Life 30.1 Playing “Dress Up” on the Reef 617
THIRD E DIT I ON

BIOLOGY THE ESSENTIALS

1 UNIT 1 Science, Chemistry, and Cells

The Scientific Study of Life

LEARNING OUTLINE
1.1 What Is Life?
1.2 The Tree of Life Includes Three Main Branches
1.3 Scientists Study the Natural World

APPLICATIONS
Burning Question 1.1 Are viruses alive?
Why We Care 1.1 It’s Hard to Know What’s Bad for You
Burning Question 1.2 Why am I here?
Investigating Life 1.1 The Orchid and the Moth

Biology Is Everywhere. Central Park is an oasis of green in New York

City, but life thrives in the city’s streets and buildings too.
©IM_photo/Shutterstock RF

Learn How to Learn S U RV E Y T H E L A NDSCAPE

Real Learning Takes Time Science, Chemistry, and Cells
You got good at basketball, running, dancing, art, music, or video games by
putting in lots of practice. Likewise, you will need to commit time to your Life is the study of Biology
biology course if you hope to do well. To get started, look for the Learn How
consists carry
to Learn tip in each chapter of this textbook. Each hint is designed to help
of units out the
you use your study time productively. With practice, you’ll discover that all called functions of
concepts in biology are connected. The Survey the Landscape figure in every
chapter highlights each chapter’s place in the “landscape” of the entire unit. ATP makes Respiration
Use it, along with the more detailed Pull It Together concept map in the chapter Cells uses
summary, to see how each chapter’s content fits into the unit’s big picture.
consist of Carbohydrates
makes
Molecules include Proteins Photosynthesis

consist of encodes such as

catalyze the
Atoms DNA Enzymes cell’s chemical
reactions, including

Organisms from all three branches of life share a unique

combination of characteristics. Biologists are scientists who
use evidence to test hypotheses about life.
For more details, study the Pull It Together feature in the chapter summary.
What’s the Point?
Imagine a biologist. If you are
1.1 What Is Life?
like many people, you may Welcome to biology, the scientific study of life. The second half of this chapter
have pictured someone in a explores the meaning of the term scientific, but first we will consider the ques-
lab coat, carefully recording a tion, “What is life?” We all have an intuitive sense of what life is. If we see a
mouse’s reaction to some new rabbit on a rock, we know that the rabbit is alive and the rock is not. But it is
drug. But this view of biology difficult to state just what makes the rabbit alive. Likewise, in the instant after
an individual dies, we may wonder what invisible essence has transformed the
as something that happens
living into the dead.
only in a laboratory is much
One way to define life is to list its basic components. The cell is the basic
too limited. Indeed, we need
unit of life; every organism, or living individual, consists of one or more cells.
©Jeff Gynane/Getty Images RF
not even leave home to study Every cell has an outer membrane that separates it from its surroundings. This
biology. Life is in parks, back- membrane encloses the water and other chemicals that carry out the cell’s
yards, and the strips between streets and sidewalks. It’s also in functions. One of those biochemicals, deoxyribonucleic acid (DNA), is the
office buildings and restaurants, not only because we are alive informational molecule of life (figure 1.1). Cells use genetic instructions—as
but also because countless microorganisms live everywhere, encoded in DNA—to produce proteins, which enable cells to carry out their
smaller than the eye can see. The food you have eaten today functions in tissues, organs, and organ systems.
was (until recently, anyway) alive. Biology really is everywhere. A list of life’s biochemicals, however, provides an unsatisfying definition
Biology is frequently in the news, in the form of stories of life. After all, placing DNA, water, proteins, and a membrane in a test tube
about fossils, weight loss, cancer, genetics, climate change, does not create life. And a crushed insect still contains all of the biochemicals
that it had immediately before it died.
and the environment. Topics such as these enjoy frequent
media coverage because this is an exciting time to study
biology. Not only is the field changing rapidly, but its new
discoveries and applications might change your life. DNA
technology has brought us genetically engineered bacteria
that can manufacture pharmaceutical drugs—and genetically
engineered corn plants that produce their own pesticides.
One day, physicians may routinely cure inherited diseases by
supplementing faulty DNA with a functional “patch.”
This book will bring you a taste of modern biology and help
you make sense of the science-related news you see every
day. Chapter 1 begins your journey by introducing the scope of
biology and explaining how science teaches us what we know
about life.

Figure 1.1 Informational Molecule of Life. All cells contain

DNA, a series of “recipes” for proteins that each cell can make.
©SMC Images/The Image Bank/Getty Images
3
4 UNIT 1 Science, Chemistry, and Cells

CELL
ORGANELLE The fundamental
A membrane-bounded unit of life. Multicellular
structure that has a specific organisms consist of many
function within a cell. cells; unicellular organisms TISSUE
Example: Chloroplast consist of one cell. A collection of specialized cells
Example: Leaf cell that function in a coordinated
fashion. (Multicellular life only.)
MOLECULE Example: Epidermis of leaf
A group of joined atoms.
Example: DNA
ORGAN
A structure consisting
of tissues organized to
interact and carry
ATOM out specific functions.
The smallest chemical (Multicellular life only.)
unit of a type of pure Example: Leaf
substance (element).
Example: Carbon atom ORGANISM
A single living individual.
Example: One acacia tree

ORGAN SYSTEM
Organs connected
physically or chemically
POPULATION
that function together.
A group of the same species of organism
(Multicellular life only.)
living in the same place and time.
Example: Aboveground
Example: Multiple acacia trees
part of a plant

BIOSPHERE
COMMUNITY The global ecosystem;
All populations that occupy the parts of the planet
the same region. ECOSYSTEM and its atmosphere
Example: All populations The living and nonliving where life is possible.
in a savanna components of an area.
Example: The savanna

Figure 1.2 Life’s Organizational Hierarchy. This diagram applies life’s organizational hierarchy to a multicellular organism (an acacia tree). Green
arrows represent the hierarchy up to the level of the organism; blue arrows represent levels that include multiple organisms. Photos: (population): ©Gregory G.
Dimijian, M.D./Science Source; (community): ©Daryl Balfour/Gallo Images/Getty Images; (ecosystem): ©Bas Vermolen/Getty Images; (biosphere): ©StockTrek/Getty Images

What Is Life? The Tree of Life Includes Three Main Branches Scientists Study the Natural World
 Chapter 1 The Scientific Study of Life 5

In the absence of a concise definition, scientists have settled on five quali-

TABLE 1.1 Characteristics of Life: A Summary
ties that, in combination, constitute life. Table 1.1 summarizes them, and the rest
of section 1.1 describes each one in more detail. An organism is a collection of Characteristic Example
structures that function together and exhibit all of these qualities (see Burning
Organization Atoms make up molecules, which make up cells,
Question 1.1). Note, however, that each trait in table 1.1 may also occur in which make up tissues, and so on.
nonliving objects. A rock crystal is highly organized, but it is not alive. A fork
Energy use A kitten uses the energy from its mother’s milk
placed in a pot of boiling water absorbs heat energy and passes it to the hand
to fuel its own growth.
that grabs it, but this does not make the fork alive. A fire can “reproduce” and
grow, but it lacks most of the other characteristics of life. It is the combination Maintenance of Your kidneys regulate your body’s water
internal constancy balance by adjusting the concentration of your
of these five characteristics that makes life unique. (homeostasis) urine.

Reproduction, An acorn germinates, develops into an oak

A. Life Is Organized growth, and seedling, and, at maturity, reproduces sexually
development to produce its own acorns.
Just as the city where you live belongs to a c­ ounty, state, and nation, living
matter also consists of parts organized in a hierarchical pattern ­(figure 1.2). At Evolution Increasing numbers of bacteria survive
the smallest scale, all living structures are composed of particles called atoms, treatment with antibiotic drugs.
which bond together to form molecules. These molecules can form organelles,
which are compartments that carry out specialized functions in cells (note that
not all cells contain organelles). Many organisms consist of single cells. In
multicellular organisms such as the tree illustrated in figure 1.2, however, the
cells are organized into specialized tissues that make up organs. Multiple or-
gans are linked into an individual’s ­organ systems.
We have now reached the level of the organism, which may consist
of just one cell or of many cells organized into tissues, organs, and or-
gan systems. Organization in the living world extends beyond the level
of the individual organism as well. A population includes members of
the same species occupying the same place at the same time.
A c­ ommunity includes the populations of different species in a region,
and an ecosystem ­includes both the living and nonliving components of
an area. ­Finally, the ­biosphere consists of all parts of the planet that can
support life.
Biological organization is apparent in all life. Humans, eels, and ever-
greens, although outwardly very different, are all organized into specialized
cells, tissues, organs, and organ systems. Single-celled bacteria, although less
complex than animals or plants, still contain DNA, proteins, and other mole-
cules that interact in highly organized ways. Brain cell
An organism, however, is more than a collection of successively smaller
Interacting brain cells
parts. Emergent properties are new functions that arise from interactions
among a system’s components, much as flour, sugar, butter, and chocolate can
become brownies—something not evident from the parts themselves. F ­ igure 1.3
shows another example of emergent properties: the thoughts and memories
produced by interactions among the neurons in a person’s brain. For an emer-
gent property, the whole is greater than the sum of the parts.
Brain
Emergent properties explain why structural organization is closely tied
to function. Disrupt a structure, and its function ceases. Brain damage,
for instance, disturbs the interactions between brain cells and can inter-
fere with memory, coordination, and other brain functions. Likewise, if
a function is interrupted, the corresponding structure eventually breaks
down, much as unused muscles begin to waste away. Biological function
and form are interdependent.
Emergent property: Memory

B. Life Requires Energy Figure 1.3 An Emergent Property—From Cells to Memories.

Inside each cell, countless chemical reactions sustain life. These reactions, col- Highly branched cells interact to form a complex network in the brain.
lectively called metabolism, allow organisms to acquire and use energy and nu- Memories, consciousness, and other qualities of the mind emerge
trients to build new structures, repair old ones, and reproduce. only when these cells interact in a certain way.
6 UNIT 1 Science, Chemistry, and Cells

Biologists divide organisms into broad categories, based on their source

Energy Usable energy of energy and raw materials (figure 1.4). Primary producers, also called
from
sunlight
Nutrients autotrophs, make their own food by extracting energy and nutrients from
nonliving sources. The most familiar primary producers are the plants and
microbes that capture light energy from the sun, but some bacteria can de-
Heat rive chemical energy from rocks. C­ onsumers, in contrast, obtain energy and
Heat nutrients by eating other organisms, living or dead; consumers are also called
heterotrophs (hetero- means “other”). You are a consumer, relying on energy
and atoms from food to stay alive. D
­ ecomposers are heterotrophs that absorb
energy and nutrients from wastes or dead organisms. These organisms,
which include fungi and some bacteria, recycle nutrients to the nonliving
environment.
Within an ecosystem, organisms are linked into elaborate food webs, be-
Consumers obtain ginning with primary producers and continuing through several levels of con-
energy and nutrients by sumers (including decomposers). But energy transfers are never 100% efficient;
eating other organisms.
some energy is always lost to the surroundings in the form of heat (see
Primary producers extract figure 1.4). Because no organism can use it as an energy source, heat represents
energy and nutrients from
the nonliving environment. a permanent loss from the cycle of life. All ecosystems therefore depend on a
continuous stream of energy from an outside source, usually the sun. i food
webs, section 19.6A
Soil
Heat
C. Life Maintains Internal Constancy
Decomposers are consumers The conditions inside cells must remain within a constant range, even if the
that obtain nutrients from dead
organisms and organic wastes. surrounding environment changes. For example, a cell must maintain a certain
temperature; it will die if it becomes too hot or too cold. The cell must also take
Figure 1.4 Life Is Connected. All organisms extract energy and in nutrients, excrete wastes, and regulate its many chemical reactions to prevent
nutrients from the nonliving environment or from other organisms. a shortage or surplus of essential ­substances. H
­ omeostasis is this state of inter-
Decomposers recycle nutrients back to the nonliving environment. nal constancy, or equilibrium.
At every stage along the way, heat is lost to the surroundings. Because cells maintain homeostasis by counteracting changes as they oc-
cur, organisms must be able to sense and react to stimuli. To illustrate this idea,
consider the mechanisms that help maintain your internal temperature at about
37°C (figure 1.5). When you go outside on a cold day, you may begin to shiver;
heat from these muscle movements warms the body. In severe cold, your lips
and fingertips may turn blue as your circulatory system sends blood away from
your body’s surface. Conversely, on a hot day, sweat evaporating from your
skin helps cool your body.

D. Life Reproduces, Grows, and Develops

Organisms reproduce, making other individuals that are similar to themselves
(figure 1.6). Reproduction transmits DNA from generation to generation; this
genetic information defines the inherited characteristics of the offspring.
Reproduction occurs in two basic ways: asexually and sexually. In ­asexual
reproduction, genetic information comes from only one parent, and all off-
spring are virtually identical. One-celled organisms such as bacteria reproduce
asexually by doubling and then dividing the contents of the cell. Many multi-
cellular organisms also reproduce asexually. A strawberry plant, for instance,
produces “runners” that sprout leaves and roots, forming new plants that are
identical to the parent. Fungi produce countless asexual spores, visible as the
green, white, or black powder on moldy bread or cheese. Some animals, in-
a. b.
cluding sponges, reproduce asexually when a f­ragment of the parent animal
Figure 1.5 Temperature Homeostasis. (a) Shivering and detaches and develops into a new ­individual.
(b) sweating are responses that maintain body temperature within an In sexual reproduction, genetic material from two parents unites to form
optimal range. an offspring, which has a new combination of inherited traits. By mixing genes
(a): ©Design Pics/Kristy-Anne Glubish RF; (b): ©John Rowley/Getty Images RF at each generation, sexual reproduction results in tremendous diversity in a

What Is Life? The Tree of Life Includes Three Main Branches Scientists Study the Natural World
 Chapter 1 The Scientific Study of Life 7

Miniglossary | Reproduction
Asexual  nly one parent passes genetic information
O
reproduction to offspring; produces genetically identical
offspring (except for mutations); adaptive in
unchanging environments
Sexual  enetic material from two parents combines
G
reproduction to form offspring; produces genetically
variable offspring; adaptive in changing
environments

a. b.

Figure 1.6 Asexual and Sexual Reproduction. (a) Identical plantlets

develop along the runners of a wild strawberry plant. (b) Two swans protect their
offspring, the products of sexual reproduction.
(a): ©Dorling Kindersley/Getty Images; (b): ©Jadranko Markoc/flickr/Getty Images RF

population. Genetic diversity, in turn, enhances the chance that some individu-
als will survive even if conditions change. Sexual reproduction is therefore a
very successful strategy, especially in an environment where conditions change
frequently; it is ex­tremely common among plants, animals, and fungi.
If each offspring is to reproduce, it must grow and develop to adult-
hood. Each young swan in figure 1.6, for example, started as a single fertil-
ized egg cell. That cell divided over and over, developing into an embryo.
Continued cell division and specialization yielded the newly hatched swans,
which will eventually mature into adults that can also reproduce—just like
their parents.

E. Life Evolves
One of the most intriguing questions in biology is how organisms become so
well-suited to their environments. A beaver’s enormous front teeth, which
never stop growing, are ideal for gnawing wood. Tubular flowers have ex-
actly the right shapes for the beaks of their hummingbird pollinators. Some
organisms have color patterns that enable them to fade into the background
(figure 1.7).
These examples, and countless others, illustrate adaptations. An a­ daptation
is an inherited characteristic or behavior that enables an o­ rganism to survive
and reproduce successfully in its environment.
Where do these adaptive traits come from? The answer lies in natural se-
lection. The simplest way to think of natural selection is to consider two facts.
First, populations produce many more offspring than will survive to reproduce;
these organisms must compete for limited resources such as food and habitat.
A single mature oak tree may produce thousands of acorns in one season, but
only a few are likely to germinate, develop, and reproduce. The rest die. Second,
no organism is exactly the same as any other. ­Genetic ­mutations—changes in
an organism’s DNA sequence—generate variability in all organisms, even
those that reproduce asexually.
Of all the offspring in a population, which ones will outcompete the ­others
and live long enough to reproduce? The answer is those with the best a­ daptations
to the current environment; conversely, the poorest competitors are most likely Figure 1.7 Hiding in Plain Sight. This pygmy seahorse is barely
to die before reproducing. A good definition of natural ­selection, then, is the visible in its coral habitat, thanks to its unique body shape, skin color,
enhanced reproductive success of ­certain individuals from a population based and texture.
on ­inherited characteristics. ©Mark Webster Wwwphoteccouk/Getty Images
8 UNIT 1 Science, Chemistry, and Cells
Burning Question 1.1
Are viruses alive?
Ebola virus
TEM (false color) 300 nm
Source: CDC/Frederick Murphy
Many people combine viruses and bacteria into the category of “germs.”
This terminology makes sense because both viruses and bacteria are
microscopic and can cause disease. But they are not the same thing.
A bacterium is a cell, complete with a membrane, cytoplasm,
DNA, and proteins. Viruses, on the other hand, are not cells. Instead,
the simplest virus consists of a protein shell surrounding a small
amount of genetic material. Other viruses have more complex fea-
tures, but no virus has the structure or functions of a cell.
Most biologists do not consider a virus to be alive because it does
not metabolize, respond to stimuli, or reproduce on its own. Instead, a
virus must enter a living host cell to manufacture more of itself.
Nevertheless, viruses do have some features in common with
life, including evolution. Each time a virus replicates inside a host
cell, random mutations occur in its genetic information. The resulting
variability among the new viruses is subject to natural selection. That
is, some variants are better than others at infecting and replicating in
host cells. Many mutant viruses die out, but others pass their success-
ful gene versions to the next generation. Over time, natural selection
shapes the genetic composition of each viral population.
Submit your burning question to
marielle.hoefnagels@mheducation.com
Figure 1.8 Natural Selection. Staphylococcus
aureus (commonly called “staph”) is a bacterium
that causes skin infections. A bacterium undergoes
a random genetic mutation that (by chance) makes
the cell resistant to an antibiotic. The presence of
the antibiotic increases the reproductive success
of the resistant cell and its offspring. After many
generations, nearly all of the bacteria in the Staphylococcus aureus
population are antibiotic-resistant. Conversely, if before mutation
antibiotics are absent, the antibiotic-resistance trait
remains rare. Generation 1
What Is Life? The Tree of Life Includes Three Main Branches
Figure 1.8 shows one example of natural selection. The illustration shows
a population of bacteria in which a mutation has occurred in one cell. If antibi-
otics are present, the drug kills most of the unmutated cells. The mutated cell,
however, is unaffected and can reproduce. After many generations of exposure
to the drug, antibiotic-resistant cells are common.
The same principle applies to all populations. In general, individuals with
the best combinations of genes survive and reproduce, while those with less
suitable characteristics fail to do so. Over many generations, individuals with
adaptive traits make up most or all of the population.
But the environment is constantly changing. Continents shift, sea levels
rise and fall, climates warm and cool. What happens to a population when the
selective forces that drive natural selection change? Only some organisms sur-
vive: those with the “best” traits in the new environment. Features that may
once have been rare become more common as the reproductive success of in-
dividuals with those traits improves. ­Notice, however, that this outcome de-
pends on variability within the population. If no individual can reproduce in
the new environment, the species may go extinct.
Natural selection is one mechanism of evolution, which is a change in the
genetic makeup of a population over multiple generations. Although evolution
can also occur in other ways, natural selection is the mechanism that selects for
adaptations. Charles Darwin became famous in the 1860s after he published a
book describing the theory of evolution by natural selection; another naturalist,
Alfred Russel Wallace, independently developed the same idea at around the
same time.
Evolution is the single most powerful idea in biology. As unit 3 describes
in detail, the similarities among existing organisms strongly suggest that all
species descend from a common ancestor. Evolution has molded the life that
has populated the planet since the first cells formed almost 4 billion years ago,
and it continues to act today.
1.1 Mastering Concepts
1. List life’s organizational hierarchy from smallest to largest, starting
with atoms and ending with the biosphere.
2. The bacteria in figure 1.8 reproduce asexually, yet they are
evolving. What is their source of genetic variation?
Antibiotic present
Time Reproduction
and Antibiotic absent
selection
Mutation
occurs (red)
Generation 2 Multiple generations later
Scientists Study the Natural World
 Chapter 1 The Scientific Study of Life 9

Prokaryotes DOMAIN EUKARYA

1.2 The Tree of Life Includes Three DOMAIN DOMAIN

Animals
Fungi
Main Branches BACTERIA ARCHAEA
Plants
Biologists have been studying life for centuries, documenting the existence of
everything from bacteria to blue whales. An enduring problem has been how
to organize the ever-growing list of known organisms into meaningful catego-
ries. Taxonomy is the science of naming and classifying organisms.
The basic unit of classification is the species, which designates a distinc- Protista
tive “type” of organism. Closely related species are grouped into the same
genus. Together, the genus and a specific descriptor denote the unique, two-
word scientific name of each species. A human, for example, is Homo sapiens. Common ancestor of all life
(Note that scientific names are always italicized and that the genus—but not
DOMAIN BACTERIA DOMAIN ARCHAEA
the specific descriptor—is capitalized.) Scientific names help taxonomists and
other biologists communicate with one another. • Cells lack nuclei (prokaryotic) • Cells lack nuclei (prokaryotic)
But taxonomy involves more than simply naming species. Taxonomists • Most are unicellular • Most are unicellular
also strive to classify organisms according to what we know about evolutionary
relationships; that is, how recently one type of organism shared an ancestor
with another type. The more recently they diverged from a shared ancestor, the
more closely related we presume the two types of organisms to be (­figure 1.9).
Researchers infer these relationships by comparing anatomical, behavioral,
cellular, genetic, and biochemical characteristics.
Genetic evidence suggests that all species fall into one of three ­domains,
the broadest (most inclusive) taxonomic category. Figure 1.10 depicts the three TEM (false color) 1 μm SEM (false color) 1 μm
domains: Bacteria, Archaea, and Eukarya. The species in domains Bacteria
and Archaea are superficially similar to one another; all are prokaryotes, mean- DOMAIN EUKARYA
ing that their DNA is free in the cell and not confined to an organelle called a • Cells contain nuclei (eukaryotic)
nucleus. Major differences in DNA sequences separate these two domains • Unicellular or multicellular
from each other. The third domain, Eukarya, contains all species of eukaryotes, Protista (multiple kingdoms) Kingdom Animalia
which are unicellular or multicellular organisms whose cells contain a nucleus.
• Unicellular or multicellular • Multicellular
i prokaryotes and eukaryotes, section 3.2
• Autotrophs or heterotrophs • Heterotrophs (by ingestion)

More closely related to

each other than to platypus

LM 200 μm

Kingdom Fungi Kingdom Plantae

• Most are multicellular • Multicellular
Platypus Kangaroo Human
• Heterotrophs (by external • Autotrophs
digestion)

Last common ancestor

of kangaroo and human

Last common ancestor

of all mammals Figure 1.10 Life’s Diversity. The three domains of life arose
from a hypothetical common ancestor, shown at the base of the
Figure 1.9 Simple Evolutionary Tree. The common ancestor of kangaroos
evolutionary tree.
and humans lived more recently than did the common ancestor that both
Photos: (Bacteria): ©Heather Davies/SPL/Getty Images RF; (Archaea): ©Eye of Science/
groups share with a platypus. This diagram depicts one tiny twig in the overall Science Source; (Protista): ©Melba/age fotostock; (Animalia): USDA/ARS/Scott Bauer;
tree of life. (Fungi): ©Corbis RF; (Plantae): USDA/Keith Weller
10 UNIT 1 Science, Chemistry, and Cells

The species in each domain are further subdivided into kingdoms;

f­igure 1.10 shows the kingdoms within domain Eukarya. Three of these
­kingdoms—Animalia, Fungi, and Plantae—are familiar to most people.
Within each one, organisms share the same general strategy for acquiring
energy. The plant kingdom contains autotrophs, whereas fungi and animals
are consumers that differ in the details of how they obtain food. But the
fourth group of eukaryotes, the Protista, contains a huge collection of unre-
Taxonomic group Humans are in: lated species. Protista is a convenient but artificial “none of the above”
Most
Domain Eukarya
category for the many species of eukaryotes that are not plants, fungi,
inclusive or animals.
Kingdom Animalia Each kingdom is likewise divided into multiple phyla, which are in turn
divided into still smaller groups. Figure 1.11 depicts the complete taxonomic
Phylum Chordata hierarchy, which is described in more detail in section 14.6.
Class Mammalia

Order Primates 1.2 Mastering Concepts

1. If the human and kangaroo in figure 1.9 switched places, would
Family Hominidae
the evolutionary tree show different relationships among the
Genus Homo three animals from what it shows now? Why or why not?
2. How are domains related to kingdoms?
Least Species Homo sapiens
inclusive 3. List and describe the four main groups of eukaryotes.

Figure 1.11 The Taxonomic Hierarchy. The eight major levels in

the taxonomic hierarchy range from domain to species. This example
applies the hierarchy to our own species, Homo sapiens.

1.3 Scientists Study the Natural World

The idea of biology as a “rapidly changing field” may seem strange if you think
of science as a collection of facts. After all, the parts of a frog are the same now
as they were 50 or 100 years ago. But memorizing frog anatomy is not the same
as thinking scientifically. Scientists use evidence to answer questions about the
natural world. If you compare a bullfrog to a rattlesnake, for instance, can you
determine how the frog can live in water and on land, whereas the snake sur-
vives in the desert? Understanding anatomy simply gives you the vocabulary
you need to ask these and other interesting ­questions about life.

A. The Scientific Method Has Multiple

Interrelated Parts
Scientific knowledge arises from application of the scientific method, which
is a general way of using evidence to answer questions and test ideas (­figure 1.12).
Although this diagram may give the impression that science is a tedious, step-
by-step process, that is not at all true. Instead, science combines thinking, de-
tective work, collaborating with other scientists, learning from mistakes, and
noticing connections. The resulting insights have taught us everything we
know about the natural world.

Observations and Questions The scientific method begins with ob-

servations and questions. The observations may rely on what we can see,
hear, touch, taste, or smell, or they may be based on existing knowledge and
experimental results. Often, a great leap forward happens when one person
makes connections between previously unrelated observations. Charles
Darwin, for example, developed the idea of natural selection by combining
his understanding of Earth’s long history with his detailed observations of

What Is Life? The Tree of Life Includes Three Main Branches Scientists Study the Natural World
 Chapter 1 The Scientific Study of Life 11

Publish
Example: Rotavirus is common and
causes serious illness; vaccines are
Peer often cost-effective ways to prevent
review illness.
Make
observations
Example: Is the rotavirus
Ask a question vaccine effective?
Example: Results suggest Draw
that the vaccine was safe conclusions
and effective in this study.

Consult prior
knowledge
Consult prior
knowledge

Formulate Example: The vaccine will

a hypothesis stimulate the immune system
Collect and and will therefore be associated
interpret data with reduced incidence of
rotavirus illness.
Example: Researchers
measure disease incidence Make
among children in study. predictions
Design
experiment Example: If vaccine is
effective, then vaccinated
Example: Researchers children should have lower
randomly assign 100 incidence of rotavirus illness
healthy children to a than children receiving placebo.
control group or to one
of three treatment groups.

Figure 1.12 Scientific Inquiry. The power of scientific thinking is its combined
emphasis on logic and creativity. This figure is simplified; in reality, teams of
scientists work on multiple “steps” simultaneously. In addition, note that data
may come from observations of the natural world or from experimentation; this
example uses an experiment.

organisms. ­Another great advance occurred decades later, when biologists

realized that mutations in DNA generate the variation that Darwin saw but
could not explain.

Hypothesis and Prediction A hypothesis is a tentative explanation for

one or more observations. The hypothesis is the essential “unit” of scientific
inquiry. To be useful, the hypothesis must be testable—that is, there must be a
way to collect data that can support or reject it. Interestingly, a hypothesis can-
not be proven true, because future discoveries may contradict today’s r­esults.
Nevertheless, a hypothesis becomes widely accepted when multiple lines of
evidence support it, no credible data refute it, and plausible alternative hypoth-
eses have been rejected.
A hypothesis is a general statement that should lead to specific p
­ redictions.
Often, a prediction is written as an if-then statement. As a simple example,
suppose you hypothesize that your lawn mower stopped working because it ran
out of gas. A reasonable prediction would be, “If I put fuel into the tank, then
my lawn mower should start.” ©McGraw-Hill Education/Jill Braaten, photographer
12 UNIT 1 Science, Chemistry, and Cells

Data Collection Investigators draw conclusions based on data (­figure 1.13).

The data may come from careful observations of the natural world, an approach
called discovery science. The National Audubon S ­ ociety’s annual Christmas
Bird Count is a case in point. For more than a century, thousands of “citizen
scientists” have documented the ups and downs of hundreds of bird species
nationwide. Another way to gather data is to carry out an experiment to test
a hypothesis under controlled conditions (section 1.3B explores experi-
mental ­design in more detail).
Discovery and experimentation work hand in hand. Consider the
well-known connection b­ etween cigarettes and lung cancer. In the late
1940s, scientists showed that smokers are far more likely than nonsmok-
ers to develop cancer. Since that time, countless laboratory experiments
have revealed how the chemicals in tobacco damage living cells.

Analysis and Peer Review After collecting and interpreting data,

investigators decide whether the evidence supports or falsifies the hypoth-
esis. Often, the most interesting results are those that are unexpected, be-
cause they provide new observations that force scientists to rethink their
hypotheses; figure 1.12 shows this feedback loop. Science advances as new
information arises and explanations continue to improve.
a. Once a scientist has enough evidence to support or reject a hypothesis, he
or she may write a paper and submit it for publication in a scientific journal.
The journal’s editors send the paper to anonymous reviewers who are knowl-
edgeable about the research topic. In a process called peer ­review, these scien-
tists independently evaluate the validity of the methods, data, and con­clusions.
Overall, peer review ensures that journal articles—the tangible products of the
global scientific conversation—are of high quality.

B. An Experimental Design Is a Careful Plan

Scientists test many hypotheses with the help of experiments (table 1.2). An
experiment is an investigation carried out in controlled conditions. This sec-
tion considers a real study that tested the hypothesis that a new vaccine protects
against a deadly virus. The virus, called rotavirus, causes severe diarrhea and
takes the lives of hundreds of thousands of young children each year. An effec-
tive, inexpensive vaccine would prevent many childhood deaths.

Sample Size One of the most important decisions that an investigator

makes in designing an experiment is the sample size, which is the number of
individuals assigned to each treatment. The sample size in the rotavirus study
b.
was approximately 100 infants per treatment. In general, the larger the sample
Figure 1.13 Different Types of Science. (a) Scientists track the
size, the more credible the results of a study.
number of migratory birds that visit a wildlife refuge each year—an
Variables A systematic consideration of variables is also important in ex-
example of discovery science. (b) Controlled experiments can help
perimental design. A variable is a changeable element of an experiment, and
food scientists objectively compare techniques for roasting or
there are several types. The independent variable is the factor that an investi-
brewing coffee.
gator directly manipulates to determine whether it causes another variable to
(a): U.S. Fish & Wildlife Service/J&K Hollingsworth; (b): ©Corbis/age fotostock RF
change. In the rotavirus study, the independent variable was the dose of the
vaccine. The dependent variable is any response that might depend on the
value of the independent variable, such as the number of children with
­rotavirus-related illness during the study period.
A standardized variable is anything that the investigator holds constant
for all subjects in the experiment, ensuring the best chance of detecting the effect
of the independent variable. Because rotavirus infection is most common among
very young children, the test of the new vaccine included only infants younger
than 12 weeks. Furthermore, vaccines work best in people with healthy immune

What Is Life? The Tree of Life Includes Three Main Branches Scientists Study the Natural World
 Chapter 1 The Scientific Study of Life 13

TABLE 1.2 Experimental Design: A Summary

Component Definition Example
Sample size The number of subjects in a treatment group or control group 100 infants

Variables
Independent variable A variable that an investigator manipulates to determine whether it Dose of vaccine
influences the dependent variable
Dependent variable A variable that an investigator measures to determine whether it is Number of children with illness caused by rotavirus
affected by the independent variable
Standardized variable Any variable that an investigator intentionally holds constant for all Age and health of children in study
subjects in an experiment, including the control group

Control Basis for comparison to treatment group(s); control subjects may Placebo lacking active ingredient in vaccine
remain untreated or receive a placebo

systems, so the study excluded infants who were ill or had weak immunity. Age
and health were therefore among the study’s standardized variables.

Controls Well-designed experiments compare one or more groups undergo-

ing treatment to a group of “normal” (untreated) individuals. The experimental
control is the untreated group, and it is important because it provides a basis
for comparison in measuring the effect of the independent variable. I­ deally, the
only difference between the control and any other experimental group is the
one factor being tested.
Experimental controls may take several forms. Sometimes, the control
group simply receives a “zero” value for the independent variable. If a gar-
dener wants to test a new fertilizer in her garden, she may give some plants a
lot of fertilizer, others only a little, and still others—the control plants—none.
In medical research, a control group might receive a placebo, an inert sub-
stance that resembles the treatment given to the experimental group. The con-
trol i­nfants in the rotavirus study received a placebo that contained all compo-
nents of the vaccine except the active ingredient.
30
Incidence of illness (cases/100 child-years)

Statistical Analysis Once an experiment is complete, the investigator 25.86

Any rotavirus illness
Severe rotavirus illness
compiles the data and decides whether the results support the hypothesis. Look 25
at the results in figure 1.14. Did the vaccine prevent illness, or do the data sim-
Dependent variable

ply reflect random chance? The researchers concluded that the vaccine was 20
effective, but only after applying a statistical analysis.
Researchers may use many different statistical tests, depending on the type 15 14.46
of data. All such tests consider both variation and sample size to estimate the
probability that the results arose purely by chance. If this probability is low, 10
then the results are considered statistically significant. Appendix B shows 6.19 6.86
how scientists use error bars and other notation to illustrate statistical signifi- 5
2.15 2.15
cance in graphs. 0 0
0
Placebo Low Medium High
C. Theories Are Comprehensive Explanations (control)
Dose of vaccine
Outside of science, the word theory is often used to describe an opinion or a
hunch. For instance, immediately after a plane crash, experts offer their “theo- Independent variable
ries” about the cause of the disaster. These tentative explanations are really
untested hypotheses. Figure 1.14 Vaccine Test. In this test of a new vaccine, the
In science, the word theory has a distinct meaning. Like a hypothesis, a independent variable was the vaccine dose; control infants received
theory is an explanation for a natural phenomenon, but a theory is typically a placebo. The statistical analysis (not shown) suggests that all
broader in scope than a hypothesis. For example, the germ theory—the idea vaccine doses prevented rotavirus illness when compared with the
that some microorganisms cause human disease—is the foundation for medical placebo.
14 UNIT 1 Science, Chemistry, and Cells
Nectar tubes
Figure 1.15 Prediction Confirmed. When Charles Darwin saw this
Madagascar orchid, he predicted that its pollinator would have long,
thin mouthparts that could reach the bottom of the elongated nectar
tubes. He was right; the unknown pollinator turned out to be a moth
with an extraordinarily long tongue.
©Kjell Sandved/Alamy Stock Photo
Miniglossary | Scientific Knowledge
Hypothesis A tentative, falsifiable explanation for one or
more observations. If tests support a hypothe­sis,
it may be incorporated into broader theories.
Outside of science, hypothesis is used
interchangeably with theory.
Theory A falsifiable, comprehensive explanation for
a natural phenomenon, typically backed
with many lines of evidence. Nonscientists often
use the term as a synonym for opinion or hunch.
Fact A repeatable observation that everyone can
agree on. Nonscientists often view facts as
the only reliable presentation of reality,
but a collection of facts by itself does not
explain anything. Theories provide these
explanations.
What Is Life? The Tree of Life Includes Three Main Branches
microbiology. Individual hypotheses relating to the germ theory are much nar-
rower, such as the suggestion that rotavirus causes illness. Not all theories are
as “large” as the germ theory, but they generally encompass multiple hypoth-
eses. Note also that the germ theory does not imply that all microbes make us
sick or that all illnesses have microbial causes. But it does explain many types
of disease.
A second difference between a hypothesis and a theory is acceptance and
evidence. A hypothesis is tentative, whereas theories reflect broader agree-
ment. This is not to imply that theories are not testable; in fact, the opposite is
true. Every scientific theory is potentially falsifiable, meaning that a particular
set of observations could prove the theory wrong. The germ theory remains
widely accepted because many observations support it and no reliable tests
have disproved it. The same is true for the theory of evolution and many other
scientific theories.
Another quality of a scientific theory is its predictive power. A good theo-
ry not only ties together many existing observations, but also suggests predic-
tions about phenomena that have yet to be observed. Both Charles Darwin and
naturalist Alfred Russel Wallace used the theory of evolution by natural selec-
tion to predict the existence of a moth that could pollinate orchid flowers with
unusually long nectar tubes (figure 1.15). Decades later, scientists discovered
the long-tongued insect (see Investigating Life 1.1). A theory weakens if sub-
sequent observations do not support its predictions.
What is the relationship between facts and a theory? One definition of the
word fact is “a repeatable observation that everyone can agree on.” It is a fact
that a dropped pencil falls toward the ground; no reasonable person disagrees
with that statement. Gravity is a fact; gravitational theory explains the forces
that cause pencils and other objects to fall.
Biologists also consider biological evolution to be a fact. Yet the phrase
“theory of evolution” persists, because evolution is both a fact and a theory.
Both terms apply equally well. The evidence for genetic change over time is so
persuasive and comes from so many different fields of study that to deny the
existence of evolution is unrealistic. Nevertheless, biologists do not understand
everything about how evolution works. Many questions about life’s history
remain, but the debates swirl around how, not whether, evolution occurs.
D. Scientific Inquiry Has Limitations
Scientific inquiry is neither foolproof nor always easy to implement. One prob-
lem is that experimental evidence may lead to multiple interpretations, and
even the most carefully designed experiment can fail to provide a definitive
answer (see Why We Care 1.1). Consider the observation that animals fed large
doses of vitamin E live longer than similar animals that do not ingest the vita-
min. So, does vitamin E slow aging? Possibly, but excess v­ itamin E also causes
weight loss, and other research has connected weight loss with longevity. Does
vitamin E extend life, or does weight loss? The ­experiment alone does not
distinguish between these possibilities.
Another limitation is that researchers may misinterpret observations or
­experimental results. For example, centuries ago, scientists sterilized a b­ ottle
of broth, corked the bottle shut, and observed bacteria in the broth a few days
later. They concluded that life arose directly from the broth. The correct expla-
nation, however, was that the cork did not keep airborne bacteria out. Although
scientists may make mistakes in the short term, science is self-correcting in the
long run because it ­remains open to new data and new interpretations.
A related problem is that the scientific community may be slow to accept
new evidence that suggests unexpected conclusions. Every investigator should
Scientists Study the Natural World
 Chapter 1 The Scientific Study of Life 15

Why We Care 1.1 | It’s Hard to Know What’s Bad for You
You have probably heard reports that a
food previously considered healthy is
actually bad for you, or vice versa. These
conflicting reports may tempt you to mistakenly conclude
that scientific studies are no better than guesswork.
Instead, the problem lies in the fact that some questions
are extremely hard to answer. Take, for example, the
artificial sweetener saccharin (see Why We Care 2.2). In
1977, the U.S. Food and Drug Administration (FDA)
proposed a ban on saccharin, based on a handful of
studies suggesting that the sweetener caused bladder
cancer in rats. Congress opted to require warning labels
on products containing saccharin. In 1991, the FDA
withdrew its proposed ban, and in 1998, saccharin was
rated as “not classifiable as to its carcinogenicity to humans.”
Two years later, legislation removed the warning label requirement. ©Vitalii Hulai/
This tangled history raises an important issue: Why Shutterstock RF
can’t science reply yes or no to the seemingly simple question of whether
saccharin is bad for you? To understand the answer, consider one of the
studies that prompted the FDA to propose the ban on saccharin in the first connection in humans instead. Unfortunately, however, documenting a link
place. Researchers divided 200 rats into two groups. The control animals between any food and cancer in people is extremely difficult. One strategy
ate standard rodent chow, whereas the experimental group got the same might be to measure the incidence of cancer in saccharin users versus
Male rats Female rats
30 30
27
Saccharin-fed
25 25 Controls
% with tumors
food supplemented with saccharin. At reproductive maturity the
animals were bred, and the researchers fed the offspring the
same dose of saccharin throughout their lives as well. To
measure the incidence of cancer, they counted the tumors in
both generations of rats for 24 months or until the rats died,
whichever came first. Figure 1.A summarizes the results.
At first glance, the conclusion seems inescapable:
Saccharin causes cancer in male lab rats. But closer study
reveals several hidden complexities that make the data
hard to interpret. First, the dose of saccharin was huge: 5%
of the rats’ diets, for life. The equivalent dose in humans
would require drinking hundreds of cans of saccharin-
sweetened soda every day. In addition, the experimental rats
weighed much less than the control rats by the end of the study,
suggesting that high doses of the sweetener are toxic. Rather than
causing cancer directly, the saccharin may have simply made the
animals more susceptible to disease. Moreover, follow-up studies
using other animals were inconclusive.
Perhaps the scientists should have studied the saccharin–cancer
nonusers. But with so many other possible causes of cancer—smoking, poor
diet, exposure to job-related chemicals, genetic predisposition—it is difficult
to separate out just the effects of saccharin.
So what are we to make of the mixed news reports? It is hard to know, but
one thing is certain: No matter what the headlines say, one study, especially a

% with tumors

20 19 20 small one, cannot reveal the whole story.

15 15
Figure 1.A The Saccharin Scare. The studies examining the link between
10 10
saccharin and bladder cancer in rats are summarized in these graphs. Sample
5 3 5 4 sizes ranged from 36 to 49 rats per treatment.
0 0 0 0 Source: Data adapted from Office of Technology Assessment Report, October 1977, Cancer
0 0
Parents Offspring Parents Offspring Testing Technology and Saccharin, page 52.

try to keep an open mind about observations, not ­allowing biases or expecta-
tions to cloud interpretation of the results. But it is human nature to be cau-
tious in accepting an observation that does not fit what we think we know. The
careful demonstration that life does not arise from broth surprised many peo-
ple who believed that mice sprang from moldy grain and that flies came from
rotted beef. More recently, it took many years to set aside the common belief
that stress caused stomach ulcers. Today, we know that a bacterium causes
most ulcers.
Although science is a powerful tool for answering questions
about the natural world, it cannot answer questions of beauty, moral-
ity, ethics, or the meaning of life (see Burning Question 1.2). Nor can
we directly study some phenomena that occurred long ago and left
little physical evidence. Consider the many experiments that have at-
tempted to re-create the chemical ­reactions that might have produced life
on early Earth. Although the experiments produce interesting results and re-
veal ways that these early events may have occurred, we cannot know if they
accurately reflect conditions at the dawn of life.

©Stockbyte/Getty Images RF
16 UNIT 1 Science, Chemistry, and Cells
Burning Question 1.2
Why am I here?
The Burning Questions featured in each
chapter of this book came from students.
On the first day of class, I always ask stu-
dents to turn in a “Burning Question”—
anything they have always wondered about
biology. I answer most of the questions as
the relevant topics come up during the se-
mester.
Why not answer all of the questions? It is
because at least one student often asks something ©Getty Images/
like, “Why am I here?” or “What is the meaning of Photodisc RF
life?” Such puzzles have fascinated humans throughout the ages, but
they are among the many questions that we cannot approach scien-
tifically. Biology can explain how you developed after a sperm from
your father fertilized an egg cell from your mother. But no one can
develop a testable hypothesis about life’s meaning or the purpose of
human existence. Science must remain silent on such questions.
Instead, other ways of knowing must satisfy our curiosity about
“why.” Philosophers, for example, can help us see how others have
considered these questions. Religion may also provide the meaning
that many people seek. Part of the value of higher education is to help
you acquire the tools you need to find your own life’s purpose.
Submit your burning question to
marielle.hoefnagels@mheducation.com
Investigating Life 1.1 | The Orchid and the Moth
Each chapter of this book has a box that examines how ­biologists
use systematic, scientific observations to solve a different
evolutionary puzzle from life’s long history. This first in-
stallment of “Investigating Life” revisits the story of the
orchid plant pictured in figure 1.15.
In a book published in 1862, Charles Darwin wrote
that certain orchids in Madagascar have nectar tubes “eleven and a half inches
long, with only the lower inch and a half filled with very sweet nectar.” He specu-
lated that the moths that reach the nectar must have tongues “capable of exten-
sion to a length of between ten and eleven inches.” However, the pollinator had
not yet been discovered.
Alfred Russel Wallace picked up the story in a book published in 1895,
summarizing how natural selection could explain this unusual orchid. He
said that only the moths with the longest tongues would be able to reach
the nectar reward in the flower. At the same time, orchids with the deepest
nectar tubes would be pollinated most easily, because the moths would rub
against the flower’s reproductive parts while reaching for the nectar. There-
fore, moths with long tongues and orchids with deep nectar tubes “each
confer on the other an advantage in the battle of life.”
What Is Life? The Tree of Life Includes Three Main Branches
E. Biology Continues to Advance
Science is just one of many ways to investigate the world, but its strength is its
openness to new information. Theories change to accommodate new knowl-
edge. The history of science is full of long-established ideas that changed as
we learned more about nature, often thanks to new technology. People
thought that Earth was flat and at the center of the universe before inven-
tions and data analysis revealed otherwise. Similarly, biologists thought
all organisms were plants or animals until microscopes unveiled a world
of life invisible to our eyes.
Technology is the practical application of scientific knowledge.
Science and technology are therefore intimately related. For example,
thanks to centuries of scientific inquiry, we understand many of the differ-
ences between humans and bacteria. We can exploit these differences to invent
new anti­biotic drugs that kill germs without harming our own bodies. These
­antibiotics, in turn, can be useful tools that help biologists learn even more about
bacterial cells. The new scientific discoveries spawn new technologies, and so on.
Biology is changing rapidly because technology has expanded our ability
to spy on living cells, compare DNA sequences, track wildlife, and make many
other types of observations. Scientists can now answer questions about the
natural world that previous generations could never have imagined.
1.3 Mastering Concepts
1. Identify the elements of the experiment summarized in Why We
Care 1.1.
2. What is a statistically significant result?
3. What is the difference between a hypothesis and a theory, and
why are some theories regarded as facts?
4. What are some limitations of scientific inquiry?
5. Compare and contrast science and technology.
Figure 1.B Found at Last. More than 40 years after Charles
Darwin predicted its existence, scientists finally discovered
the sphinx moth that pollinates the Madagascar orchid.
©The Natural History Museum/Alamy Stock Photo
A taxonomic publication from 1903 finally vali-
dated Darwin’s and Wallace’s predictions. The authors described a moth
species with a 225-millimeter (8-inch) tongue, well-suited for sipping the
Madagascar orchid’s nectar (figure 1.B).
This story illustrates how theories lead to testable predictions and re-
flects the collaborative nature of science. Darwin and Wallace asked a simple
question: Why are these nectar tubes so long? Decades later, biologists cata-
loging the world’s insect species finally solved the puzzle.
Sources: Darwin, C. R. 1862. On the Various Contrivances by Which British and Foreign Orchids Are
Fertilised by Insects, and on the Good Effects of Intercrossing. London: John Murray, pages 197–198.
Rothschild, W., and K. Jordan. 1903. A revision of the lepidopterous family Sphingidae. Novitates
Zoologicae 9, supplement part 1, page 32.
Wallace, Alfred Russel. 1895. Natural Selection and Tropical Nature: Essays on Descriptive and
Theoretical Biology. London: MacMillan and Co., pages 146–148.
Scientists Study the Natural World
Another random document with
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 Scarcely had he uttered these words, when, seizing a palette, he
seated himself at the easel, and was soon totally absorbed in his
occupation. Hour after hour passed unheeded by Sebastian, who
was too much engrossed by the beautiful creation of his pencil,
which seemed bursting into life, to mark the flight of time. “Another
touch,” he exclaimed, “a soft shade here—now the mouth. Yes!
there! it opens—those eyes—they pierce me through!—what a
forehead!—what delicacy! Oh my beautiful—” and Sebastian forgot
the hour, forgot he was a slave, forgot his dreaded punishment—all,
all was obliterated from the soul of the youthful artist, who thought of
nothing, saw nothing, but his beautiful picture.
But who can describe the horror and consternation of the
unhappy slave, when, on suddenly turning round, he beheld all the
pupils, with the master at their head, standing beside him.
Sebastian never once dreamt of justifying himself, and with his
palette in one hand, and his brushes in the other, he hung down his
head, awaiting in silence the punishment he believed he justly
merited. For some moments a dead silence prevailed; for if
Sebastian was confounded at being caught in the commission of
such a flagrant crime, Murillo and his pupils were not less astonished
at the discovery they had made.
Murillo, having, with a gesture of the hand, imposed silence on his
pupils, who could hardly restrain themselves from giving way to their
admiration, approached Sebastian, and concealing his emotion,
said, in a cold and severe tone, while he looked alternately from the
beautiful head of the virgin to the terrified slave, who stood like a
statue before him,
“Who is your master, Sebastian?”
“You,” replied the boy, in a voice scarcely audible.
“I mean your drawing-master,” said Murillo.
“You, Senor,” again replied the trembling slave.
“It cannot be; I never gave you lessons,” said the astonished
painter.
 “But you gave them to others, and I listened to them,” rejoined the
boy, emboldened by the kindness of his master.
“And you have done better than listen—you have profited by
them,” exclaimed Murillo, unable longer to conceal his admiration.
“Gentlemen, does this boy merit punishment, or reward?”
At the word punishment, Sebastian’s heart beat quick; the word
reward gave him a little courage; but, fearing that his ears deceived
him, he looked with timid and imploring eyes towards his master.
“A reward, Senor!” cried the pupils, in a breath.
“That is well; but what shall it be?”
Sebastian began to breathe.
“Ten ducats, at least,” said Mendez.
“Fifteen,” cried Ferdinand.
“No,” said Gonzalo; “a beautiful new dress for the next holiday.”
“Speak, Sebastian,” said Murillo, looking at his slave, whom none
of these rewards seemed to move; “are these things not to your
taste? Tell me what you wish for. I am so much pleased with your
beautiful composition, that I will grant any request you may make.
Speak, then; do not be afraid.”
“Oh, master, if I dared—” and Sebastian, clasping his hands, fell
at the feet of his master. It was easy to read in the half-opened lips of
the boy and his sparkling eyes some devouring thoughts within,
which timidity prevented him from uttering.
With the view of encouraging him, each of the pupils suggested
some favor for him to demand.
“Ask gold, Sebastian.”
“Ask rich dresses, Sebastian.”
“Ask to be received as a pupil, Sebastian.”
A faint smile passed over the countenance of the slave at the last
words, but he hung down his head and remained silent.
 “Ask for the best place in the studio,” said Gonzalo, who, from
being the last pupil, had the worst light for his easel.
“Come, take courage,” said Murillo gaily.
“The master is so kind to-day,” said Ferdinand, “that I would risk
something. Ask your freedom, Sebastian.”
At these words Sebastian uttered a cry of anguish, and raising his
eyes to his master, he exclaimed, in a voice choked with sobs, “The
freedom of my father! the freedom of my father!”
“And thine, also,” said Murillo, who, no longer able to conceal his
emotion, threw his arms around Sebastian, and pressed him to his
breast.
“Your pencil,” he continued, “shows that you have talent; your
request proves that you have a heart; the artist is complete. From
this day, consider yourself not only as my pupil, but my son. Happy
Murillo! I have done more than paint—I have made a painter!”
Murillo kept his word, and Sebastian Gomez, known better under
the name of the mulatto of Murillo, became one of the most
celebrated painters in Spain. There may yet be seen in the churches
of Seville the celebrated picture which he had been found painting by
his master; also a St. Anne, admirably done; a holy Joseph, which is
extremely beautiful; and others of the highest merit.

At a crowded lecture the other evening, a young lady standing at

the door of the church was addressed by an honest Hibernian, who
was in attendance on the occasion, with, “Indade, Miss, I should be
glad to give you a sate, but the empty ones are all full.”
 Sketches of the Manners, Customs, and History
of the Indians of America.

CHAPTER V.
Peru discovered by Francisco Pizarro.—​He invites the Inca to visit
him.—​Description of the Inca.—​Rejects the Bible.—​
Treacherously seized by Pizarro.—​The Inca proposes to
ransom himself.—​The ransom brought.—​Pizarro seizes the
gold, then murders the Inca.—​Conquers Peru.

When the Spaniards first discovered the Pacific, Peru was a

mighty empire. It extended from north to south more than 2000
miles. Cuzco, the capital city, was filled with great buildings, palaces,
and temples, which last were ornamented, or covered, rather, with
pure gold. The improvements of civilized life were far advanced;
agriculture was the employment of the quiet villagers; in the cities
manufactures flourished; and science and literature were in a course
of improvement which would, doubtless, have resulted in the
discovery of letters.
Their government was a regular hereditary monarchy; but the
despotism of the emperor was restricted by known codes of law.
They had splendid public roads. That from Cuzco to Quito extended
a distance of 1500 miles or more. It passed over mountains, through
marshes, across deserts. Along this route, at intervals, were large
stone buildings, like the caravanseras of the East, large enough to
contain thousands of people. In some instances these caravanseras
were furnished with the means of repairing the equipments and arms
of the troops or travellers.
 Such was the ancient empire of Peru, when Francisco Pizarro, an
obscure Spanish adventurer, with an army of only sixty-two
horsemen and a hundred or two foot-soldiers, determined to invade
it. He, like all the other Spaniards who went out to South America,
was thirsting to obtain gold. These men, miscalled Christians, gave
up their hearts and souls to the worship of mammon, and they
committed every horrible crime to obtain riches. But the Christian
who now cheats his neighbor in a quiet way-of-trade manner, to
obtain wealth—is he better than those Spaniards? I fear not. Had he
the temptation and the opportunity, he would do as they did.
At the time Pizarro invaded Peru, there was a civil war raging
between Atahualpa, the reigning monarch, or Inca, as he was called,
and his brother Huascar. These brothers were so engaged in their
strife, that Pizarro had marched into the country without being
opposed, and entered the city of Caxamala on the 15th of
November, 1532. Here the army of the Inca met the Spaniards.
Pizarro was sensible he could not contend with such a multitude, all
well armed and disciplined, so he determined by craft to get
possession of the person of the Inca.
He sent to invite the Inca to sup with him in the city of Caxamala,
and promised then to give an account of his reasons for coming to
Peru. The simple-hearted Inca believed the Spaniards were children
of the sun. Now the Inca worshiped the sun, and thought he himself
had descended from that bright luminary. He was very anxious,
therefore, to see the Spaniards, and could not believe they meant to
injure him; so he consented to visit Pizarro.
Atahualpa took with him twenty thousand warriors, and these
were attended by a multitude of women as bearers of the luggage,
when he set out to visit the Spaniards. The person of the sovereign
was one blaze of jewels. He was borne on a litter plated with gold,
overshadowed with plumes, and carried on the shoulders of his chief
nobles. On his forehead he had the sacred tuft of scarlet, which he
wore as the descendant of the sun. The whole moved to the sound
of music, with the solemnity of a religious procession.
 The Inca putting the Bible to his ear.
When the Inca entered the fatal gates from which he was never to
return, his curiosity was his chief emotion. Forgetting the habitual
Oriental gravity of the throne, he started up, and continued standing
as he passed along, gazing with eagerness at every surrounding
object. A friar, named Valverde, now approached, bearing a cross
and a Bible. The friar commenced his harangue by declaring that the
pope had given the Indies to Spain; that the Inca was bound to obey;
that the book he carried contained the only true mode of worshiping
Heaven.
“Where am I to find your religion?” said the Inca.
“In this book,” replied the friar.
The Inca declared that whatever might be the peaceful intentions
of the Spaniards, “he well knew how they had acted on the road,
how they had treated his caciques, and burned his cottages.” He
then took the Bible, and turning over some of the leaves, put it
eagerly to his ear.
“This book,” said he, “has no tongue; it tells me nothing.” With
these words he flung it contemptuously on the ground.
The friar exclaimed at the impiety, and called on his countrymen
for revenge. The Inca spoke a few words to his people, which were
answered by murmurs of indignation. At this moment Pizarro gave
the signal to his troops: a general discharge of cannon, musketry,
and crossbows followed, and smote down the unfortunate Peruvians.
The cavalry were let loose, and they broke through the Inca’s guard
at the first shock. Pizarro rushed forward at the head of a chosen
company of shield-bearers, to seize the Inca.

Pizarro seizing the Inca.

That sovereign was surrounded by a circle of his high officers and
devoted servants. They never moved except to throw themselves
upon the Spanish swords. They saw that their prince was doomed,
and they gave themselves up to his fate. The circle rapidly thinned,
and the Inca would soon have been slain, had not Pizarro called to
his soldiers to forbear. He wished to take the Inca alive, that he might
extort gold from him for his ransom.
Pizarro, therefore, rushed forward, and, seizing the Inca by the
mantle, dragged him to the ground. The Peruvians, seeing his fall in
the midst of the Spanish lances, thought he was slain, and instantly
gave up the battle. In the force of their despair they burst through
one of the walls and fled over the open country. More than two
thousand were left dead within the gates, while not a single Spaniard
had been killed. It was a murder rather than a battle.
The Spaniards proceeded to plunder the camp of the Inca, and
he, seeing their passion for gold, offered to purchase his ransom. He
offered to cover the floor of the chamber where he was confined with
wedges of gold and silver. The Spaniards laughed at this, as they
conceived, impossible proposal. The Inca thought they despised the
small sum he had offered, and starting to his feet, he haughtily
stretched his arm as high as he could reach, and told them he would
give them that chamber full to the mark he then touched with his
hand. The chamber was twenty-two feet long, sixteen wide, and the
point he touched on the wall was nine feet high.
Pizarro accepted the proposal, and sent messengers to Cuzco to
obtain the ransom. These brought back twenty-six horse loads of
gold, and a thousand pounds’ weight of silver. The generals of the
Inca also brought additional treasures of gold and silver vessels, and
the room was filled. Pizarro grasped the treasure, and divided it
among his troops, after deducting one fifth for the king, and taking a
large share for himself.
Pizarro had promised to set the Inca at liberty; but it is probable
he never intended it. After he had, in the name of the Inca, drawn all
the gold he could from the country, he barbarously murdered the
poor Indian chief!
There is a tradition that the fate of the Inca was hastened by the
following circumstance. One of the soldiers on guard over him, wrote
the name of God on the thumb nail of the Inca, explaining to him at
the same time the meaning of the word. The Inca showed it to the
first Spaniard who entered. The man read it. The Inca was delighted;
and Pizarro appearing at the moment, the important nail was
presented to him. But Pizarro could not read! the conqueror of Peru
could not write his name; and the Inca manifested such contempt
towards him for this ignorance, that Pizarro resolved he should not
live.
After the Inca’s death, another long and bloody war, or, rather,
ravage, commenced. The Spaniards finally took Cuzco, the royal
city, plundered the temples, and desolated the land, till the
Peruvians, in despair, submitted to their chains, and became the
slaves of the Spaniards.
Since that time the Spanish power has always governed Peru, till
the revolution in 1823, when the colonists threw off the yoke of the
mother country. But, in justice to the kings of Spain, it should be
remembered that they have frequently made laws to protect their
Indian subjects in South America. Still the poor natives were often,
indeed always, cruelly oppressed by the colonists. But now the spirit
of liberality and improvement is ameliorating the condition of all the
laboring classes in the independent Republic of Peru, and the
Indians are entitled to the privileges of free citizens.

CHAPTER VI.
Indian tradition.—​Manco Capac.—​His reign.—​Religion.—​Property.—​
Agriculture.—​Buildings.—​Public roads.—​Manufactures.—​
Domestic animals.—​Results of the conquest of the country by
the Spaniards.

The Peruvians have a tradition that the city of Cuzco was founded
in this manner. The early inhabitants of the country were ignorant,
and brutal as the wild beasts of the forest, till a man and woman of
majestic form, and clothed in decent garments, appeared among
them. They declared themselves to be children of the sun, sent to
instruct and to reclaim the human race. They persuaded the savages
to conform to the laws they proposed, united them, the Indians,
together in a society, and taught them to build the city.
Manco Capac was the name of this wonderful man; the woman
was called Marna Ocollo. Though they were the children of the sun,
it seems they had been brought up very industriously; for Manco
Capac taught the Indians agriculture, and other useful arts; and
Marna Ocollo taught the women to spin and weave, and make
feather garments.
After the people had been taught to work, and had built houses
and cultivated fields, and so on, Manco Capac introduced such laws
and usages as were calculated to perpetuate the good habits of the
people. And thus, according to the Indian tradition, was founded the
empire of the Incas.
The territory was, at first, small; but it was gradually enlarged by
conquering the neighboring tribes,—merely, however, to do good by
extending the blessings of their laws and arts to the barbarians,—till
the dominions of the Inca Atahualpa, the twelfth in succession,
extended from north to south along the Pacific Ocean above 2000
miles; its breadth from east to west was from the ocean to the
Andes. The empire had continued four hundred years.
The most singular and striking circumstance in the Peruvian
government, was the influence of religion upon its genius and its
laws. The whole civil policy was founded on religion. The Inca
appeared not only as a legislator, but as the messenger of heaven.
His precepts were received as the mandates of the Deity. Any
violation of his laws was punished with death; but the people were so
impressed with the power and sacred character of their ruler that
they seldom ventured to disobey.
Manco Capac taught the Peruvians to worship the sun, as the
great source of light, of joy, and fertility. The moon and stars were
entitled to secondary honors. They offered to the sun a part of those
productions which his genial warmth had called forth from the bosom
of the earth, and his beams had ripened. They sacrificed some of the
animals which were indebted to his influence for nourishment. They
presented to him choice specimens of those works of ingenuity
which his light had guided the hand of man in forming. But the Incas
never stained the altar of the sun with human blood.
Thus the Peruvians were formed, by the spirit of the religion which
they had adopted, till they possessed a national character more
gentle than that of any other people in America.
The state of property in Peru was no less singular than that of
religion, and contributed, likewise, towards giving a mild turn of
character to the people. All the lands capable of cultivation, were
divided into three shares. One was consecrated to the sun, and the
product of it was applied to the erection of the temples, and
furnishing what was requisite towards celebrating the public rites of
religion.
The second share belonged to the Inca, or was set apart as the
provision made by the community for the support of government.
The third and largest share was reserved for the maintenance of the
people, among whom it was parcelled out. All such lands were
cultivated by the joint industry of the community.
 A state thus constituted may be considered like one great family,
in which the union of the members was so complete, and the
exchange of good offices so perceptible, as to create stronger
attachment between man and man than subsisted under any other
form of society in the new world. The Peruvians were advanced far
beyond any of the nations in America, both in the necessary arts of
life, and in such as have some title to be called elegant.
Agriculture was carried on by the Peruvians with a good deal of
skill. They had artificial canals to water their fields; and to this day
the Spaniards have preserved and use some of the canals made in
the days of the Incas. They had no plough, but turned up the earth
with a kind of mattock of hard wood. The men labored in the fields
with the women, thus showing the advance of civilization over the
rude tribes which imposed all the drudgery upon females.
The superior ingenuity of the Peruvians was also obvious in their
houses and public buildings. In the extensive plains along the Pacific
Ocean, where the sky is always serene and the climate mild, the
houses were, of course, very slight fabrics. But in the higher regions,
where rain falls and the rigor of the changing seasons is felt, houses
were constructed with great solidity. They were generally of a square
form, the walls about eight feet high, built of bricks hardened in the
sun, without any windows, and the door strait and low. Many of these
houses are still to be seen in Peru.
But it was in the temples consecrated to the sun, and in the
buildings intended for the residence of their monarchs, that the
Peruvians displayed the utmost extent of their art. The temple of
Pachacmac, together with a palace of the Inca and a fortress, were
so connected together as to form one great structure, nearly two
miles in circuit.
Still this wide structure was not a very lofty affair. The Indians,
being unacquainted with the use of the pulley and other mechanical
powers, could not elevate the large stones and bricks which they
employed in building; and the walls of this, their grandest edifice, did
not rise above twelve feet from the ground. There was not a single
window in any part of the building. The light was only admitted by the
doors; and the largest apartments must have been illuminated by
some other means.
The noblest and most useful works of the Incas, were their public
roads. They had two, from Cuzco to Quito, extending,
uninterruptedly, above fifteen hundred miles. These roads were not,
to be sure, equal to our modern turnpikes; but at the time Peru was
discovered there were no public roads in any kingdom of Europe that
could be compared to the great roads of the Incas.
The Peruvians had, likewise, made considerable advances in
manufactures and the arts which may be called elegant. They made
cloth, and they could refine silver and gold. They manufactured
earthen ware; and they had some curious instruments formed of
copper, which had been made so hard as to answer the purposes of
iron. This metal they had not discovered. If they had only understood
the working of iron and steel as well as they did that of gold and
silver, they would have been a much richer and more civilized
people.
The Peruvians had tamed the duck and the llama, and rendered
them domestic animals. The llama is somewhat larger than the
sheep, and in appearance resembles a camel. The Indians
manufactured its wool into cloth; its flesh they used for food;
moreover, the animal was employed as a beast of burden, and would
carry a moderate load with much patience and docility. The aid of
domestic animals is essential to the improvement and civilization of
human society.
In short, the Peruvians, when contrasted with the naked, indolent,
and ignorant inhabitants of the West Indian Islands, seem to have
been a comfortable, ingenious, and respectable nation. The
conquest of their country destroyed their system of government.
They were made not merely to pay tribute to their new rulers, but, far
worse, they were reduced to the condition of slaves. They were
compelled to leave the pleasant fields they used to cultivate, and
driven in crowds to the mountains in search of gold. They were
forced to labor hard, and allowed only a scanty subsistence; till,
heart-broken and despairing of any change for the better, they sunk
under their calamities and died!

An Indian girl feeding a duck. Llama carrying a burden on its back.

In a few years after Pizarro entered Cuzco, a great part of the
ancient population of Peru had been swept away, destroyed by the
avarice and cruelty of their conquerors.
 The Alligator.

I am not about to recommend this creature to you on account of

his beauty or amiable qualities. He has, in fact, too large a mouth,
and too long a tail, to be handsome, and his reputation is not of the
pleasantest kind. However, it is interesting to hear about all the
works of nature, and as this is one of the most wonderful, I shall
proceed to describe it.
Alligators live in warm climates, and spend the greater part of
their time in the water. There are four or five kinds in America, but
the most dangerous are found along the banks of the river
Mississippi. These creatures are sometimes fifteen or even twenty
feet in length; their mouths are two or three feet long and fourteen or
fifteen inches wide. Their teeth are strong and sharp, and their claws
are also very strong.
During the middle of the day the alligators are generally at rest—
lying lazily upon the shore, or in the water. Toward evening, however,
they begin to move about in search of prey, and then the roar of the
larger ones is terrific. It is louder and deeper than the lowing of the
bull, and it has all the savage wildness of the bittern’s cry. It would
seem that this bellowing could not be agreeable to anything, for as
soon as the birds and beasts hear it, they fly as if smitten with terror;
but still, when an alligator wishes to speak something loving into the
ear of another, he goes to bellowing with all his might, and this
sound, so awful to other creatures, seems very pleasant and musical
to the alligator which is thus addressed. This shows that there is a
great difference in tastes.
 THE CROCODILE.
The male alligators sometimes engage in ferocious battles. These
usually take place in shallow water, where their feet can touch the
ground. At first they only cudgel each other with their tails; but the
blows given are tremendous, and soon rouse the anger of the
parties. They then go at it with teeth and claws. The snapping,
scratching, rending and thumping, are now tremendous; the water
boils around with the struggle; streams of blood mingle with the
waves; and at last one of the combatants is actually torn in pieces by
his adversary.
The appetite of the alligator is voracious; I never heard of one that
had the dyspepsia. Nothing of the animal kind comes amiss;
mountain cat, monkey, vulture, parrot, snake-lizard, and even the
electric eel, rattlesnake, and venomous bush-master, are alike
swallowed down! Nor does it matter whether the creature be alive or
dead, save only that it seems most admired when in a putrid state. It
frequently happens that the creature will deposit an animal he has
killed in the water till partly decayed, and when most offensive to us,
it seems most delicious to the alligator.
In some of the rivers of North and South America, within the
tropics, these creatures are very numerous. They also infest the
lakes and lagoons all around the Gulf of Mexico; and it is here that
the alligator’s paradise is found. When the spring rains come these
creatures have a perfect carnival. Many fishes, birds, and animals,
are killed during the freshets, and are borne along in the floods; upon
their remains these creatures feast; and as the vulture is provided by
providence to devour and remove offal from the land, which would
otherwise infect the air and produce pestilence; so the alligators are
the scavengers of the waters, and clear away putrescence that
would otherwise render them poisonous and unapproachable to
man. So, after all, the alligator has his part to play in the great
economy of nature, and is actually very useful.
The alligator is nearly the same as the crocodile of the eastern
continent. The females lay eggs, and one of them is said to produce
a hundred in a season. They are of the size of geese eggs, and are
often eaten, being esteemed tolerable food. The eggs, being
deposited in the sand and covered up, are hatched by the heat.

Braham’s Parrot.—Parrots, like cuckoos, form their notes deep

in the throat, and show great aptitude in imitating the human voice. A
lady who admired the musical talents of Braham, the celebrated
singer, gave him a parrot, which she had taught with much care. A
person who saw it at Braham’s house, thus describes it:—“After
dinner, during a pause in the conversation, I was startled by a voice
from one corner of the room, calling out in a strong, hearty manner,
‘Come, Braham, give us a song!’ Nothing could exceed the surprise
and admiration of the company. The request being repeated and not
ananswered, the parrot struck up the first verse of God save the
King, in a clear, warbling tone, aiming at the style of Braham, and
sung it through. The ease with which the bird was taught was equally
surprising with his performance. The same lady prepared him to
accost Catalani, when dining with Mr. Braham, which so alarmed
Madame that she nearly fell from her chair. Upon his commencing
Rule Brittania, in a loud and intrepid tone, the chantress fell upon her
knees before the bird, expressing, in terms of delight, her admiration
of its talents.”
 This parrot has only been exceeded by Lord Kelly’s, who, upon
being asked to sing, replied, “I never sing on a Sunday.” “Never mind
that, Poll; come, give us a song.” “No, excuse me. I’ve got a cold—
don’t you hear how hoarse I am?” This extraordinary creature
performed the three verses entire of God save the King, words and
music, without hesitation, from beginning to end.
 Mungo Park and the Frogs.

The tales of travellers often appear to us incredible, merely

because they relate things different from our own observation and
experience. You know that there are some countries so hot that they
never have ice or snow there. Now it chanced that a man from some
northern portion of the world, happening to be in one of those hot
places, told the people, that, where he lived, the water sometimes
became solid, in consequence of the cold, and almost as hard as a
stone.
Now this was so different from the experience of the people, that
they would not credit the traveller’s story. This shows us that a thing
may be a reality, which is, at the same time, very different from our
own observation and experience.
Mungo Park was a famous traveller in Africa. He went into
countries where no white man had been before, and he saw places
which no white man had seen. He tells us many curious things, but
perhaps nothing is more amusing than what he says about the frogs.
At a certain place that he visited, he went to a brook to let his horse
drink; but what was his surprise to find it almost covered with frogs,
who kept bobbing up and down, so that his horse was afraid to put
his nose into the water. At last Mr. Park was obliged to take a bush
and give the frogs a flogging, before he could make them get out of
the way so as to let his poor beast quench his thirst.
 A Child lost in the Woods.

The Bangor Whig of the 11th of June contains an affecting

account of a search made at Linnæus, in the Aroostook country, for a
little girl of nine years, the daughter of Mr. David W. Barbar, who, on
the 4th, was sent through the woods to a neighbor’s, half a mile
distant, to borrow a little flour for breakfast. Not returning that day,
the next morning about forty of the neighbors set out to hunt for her,
but spent the day without success. The next day sixty searched the
woods, with no better fortune. The following morning between two
and three hundred of the settlers assembled early, anxious and
fearful for the safety of the lost child.
“The company set out,” says the Whig, “for a thorough and a last
search. The child had been in the woods three days and nights, and
many hearts were sunk in despondency at the utter hopelessness of
finding it alive. But to learn its fate or restore it was the determined
purpose of each. Half the day had been expended in advancing into
the forest. It was time for returning; but who could think of doing so
while an innocent child might be wandering but a few rods in
advance? On the company pushed, still deeper into the dense wilds.
The sun had reached the meridian, and was dipping down toward
the west. It seemed vain to look farther, and slowly and heavily those
stout-hearted men brushed a tear from their cheeks, gave up all as
lost, and, as their hearts seemed to die within them, commenced
their return. The line was stretched to include a survey of the
greatest possible ground; not a bush or tree, where it was possible
for a child to be concealed, within the limits of the line, was passed
without diligent search. Those at the extremities of the lines tasked
themselves to the utmost in examining the woods beyond the lines.
They had travelled for some time, when, at the farthest point of
vision, the man on one flank thought he saw a bush bend. He ran