TABLE 20

Micro-organism-specific media
Micro-organism Media Author

Bacteria (general) Rhizobium

Thorntons agar medium CRYEMA (Congo red yeast extract mannitol agar) Norris and Date medium

Thornton (1922) Vincent (1970) Norris & Date (1976) Jensen (1942) Ashby (1907) Becking (1959) Baldini & Dobereiner (1980) Dobereiner, Marriel & Nery (1976) Okon, Albrecht & Burris (1977) Pikovskaya (1948)

Azotobacter

Jensens N-free medium Ashbys medium Beijerinckias medium

Azospirillum

Semi solid malate medium Nitrogen free bromothymol blue medium Okons modified medium

PSMs

Pikovskaya medium

The composition and method of preparation of the media listed in Table 20 are described below. Thorntons agar media The composition of the medium is: mannitol: 1.0 g; asparagine: 0.5 g; K2HPO 4: 1.0 g; KNO 3: 0.5 g; MgSO 4.7H 2O: 0.2 g; CaCl 2: 0.1 g; NaCl: 0.1 g; FeCl 3: 0.002 g; agar: 15.0 g; distilled water: 1 000 ml. To prepare the medium, dissolve phosphate, nitrate and asparagine in distilled water, add magnesium sulphate, calcium chloride, sodium chloride and ferric chloride. Add agar and dissolve, heat at 100 C, and filter. Add mannitol, and cool to 60 C. Adjust the pH to 7.4 with bromothymol blue, and autoclave at 121 C for 30 minutes at 1-1.25 kg/cm 2 pressure. Cool, and store for use. Congo red yeast extract mannitol agar medium The composition of the medium is: K2HPO 4: 0.5 g; MgSO 4.7H 2O: 0.2 g; NaCl: 0.1 g; mannitol: 10 g; yeast extract: 1 g;

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Guide to laboratory establishm ent for plant nutrient

agar: 15.0 g; distilled water: 1 litre; 1 percent aqueous Congo red: 2.5 ml. To prepare the medium, dissolve the salts in water, add agar, adjust the pH to 7.0, and autoclave (as above). Glucose peptone agar medium The composition of the medium is: glucose: 5.0 g; peptone: 10.0 g; agar: 15.0 g; distilled water: 1 litre. To prepare the medium, dissolve the salts in water, add agar, adjust the pH to 7.0, and autoclave (as above). Norris and Date liquid medium The composition of the medium is: mannitol: 10.0 g; yeast extract: 1 g; K2HPO 4: 0.5 g; MgSO 4.7H 2O: 0.8 g; NaCl: 0.2 g; FeCl 3.6H 2O: 0.01 g; distilled water: 1 litre. To prepare the medium, dissolve the salts in water, adjust the pH to 7.0, and autoclave (as above). Jensens N-free medium The composition of the medium is: sucrose: 20.0 g; K2HPO 4: 1.0 g; MgSO 4.7H 2O: 0.5 g; NaCl: 0.5 g; FeSO 4: 0.1 g; CaCO 3: 2.0 g; agar: 15.0 g; distilled water: 1 000 ml. To prepare the medium, dissolve the salts in water, add agar, adjust the pH to 7.0, and autoclave (as above). Ashbys medium The composition of the medium is: mannitol: 20.0 g; K2HPO 4: 0.2 g;

Chapter 7 - Biofertilizer assay and production

MgSO 4: 0.2 g; NaCl: 0.2 g; K2SO 4: 0.1 g; CaCO 3: 5.0 g; agar: 15.0 g; distilled water: 1 000 ml. To prepare the medium, dissolve the salts in water, add agar, adjust the pH to 7.0, and autoclave (as above). Beijerinckia medium The composition of the medium is: sucrose: 20.0 g; KH 2PO 4: 0.8 g; K2HPO 4: 0.2 g; MgSO 4.7H 2O: 0.5 g; FeCl 3: 0.1 g; Na 2MoO 4: 0.005 g; agar: 15 g; distilled water: 1 000 ml. To prepare the medium, dissolve the salts in water, add agar, adjust the pH to 6.5, and autoclave (as above). Semi-solid malate medium The composition of the medium is: malic acid: 5.0 g; K2HPO 4: 0.5 g; MgSO 4.7H 2O: 0.2 g; NaCl: 0.1 g; CaCl 2: 0.02 g; Na 2MoO 4.2H 2O: 0.002 g; MnSO 4.H 2O: 0.01 g; KOH: 4.5 g; biotin: 0.1 mg; Fe-EDTA (1.64 percent): 4.0 ml; bromothymol blue: 3.0 ml (0.5 percent alcoholic solution); distilled water: 1 000 ml. To prepare the medium, dissolve the salts in water, adjust the pH to 6.8, and autoclave (as above). Nitrogen-free bromothymol blue medium The composition of the medium is: malic acid: 5.0 g; KOH: 4.0 g; K2HPO 4: 0.5 g;