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CHPT 4 ENZYMES Lecture Notes (Teacher)
CHPT 4 ENZYMES Lecture Notes (Teacher)
Name: ________________________ ( )
Class: _______
Date: _____________
SOURCES
o Enzymes may be extracted from any living organism.
Sources include bacteria, fungi, yeast, plants and
animals.
APPLICATIONS
o DETERGENT: We use enzymes in our
everyday life for example in detergents
containing proteases and lipases to remove
stubborn stains.
1
CHAPTER 4: ENZYMES
o ANIMAL FEED: Enzymes are added to animal feed to
increase digestibility.
USES OF ENZYMES
o BREAKDOWN
Enzymes are used to breakdown complex
substances to simpler substances, for example
the oxidation of glucose to release energy and
form carbon dioxide and water.
o SYNTHESIS
Other enzymes are also used to synthesize
complex substances from simpler ones, for
example the build up of proteins from amino
acids.
CLASSIFICATION
Enzymes are classified according to the chemical reactions
they catalyze.
ENZYMES SUBSTRATE
CARBOHYDRASE carbohydrate
CELLULASE cellulose
PROTEASE protein
LIPASE lipids
AMYLASE starch
CHARACTERISTICS
1. ALTER OR SPEED UP THE RATE OF REACTION
2
CHAPTER 4: ENZYMES
Enzymes alter or speed up the rate of a chemical
reaction.
2. POTENT
Enzymes are very potent. They remain
unchanged at the end of the chemical reaction
and can be reused. A small amount of enzyme
can bring about a large amount of chemical
reaction.
3. SPECIFIC
Enzymes are very specific in their action i.e. protease
acts only on proteins, lipase acts only on lipids and not
starch.
4. REVERSIBLE REACTIONS
Enzymatic reactions are reversible:
A+B C+D
3
CHAPTER 4: ENZYMES
o However, beyond the optimum temperature, enzyme
activity decreases until the enzyme is completely
denatured (i.e. no enzyme activity).
Enzyme
activity
most active
Optimum
temperature
2. pH
o Enzymes are affected by the acidity or alkalinity of
the solutions in which they work.
o Different enzymes have different optimum pH. Some
enzymes work best in slightly acidic solutions (e.g.
pepsin and rennin in the stomach) while others
require slightly alkaline solutions (e.g. intestinal
enzymes).
o Extreme changes in pH denature the enzymes.
4
CHAPTER 4: ENZYMES
Enzyme
activity
most active
Optimum pH
3. SUBSTRATE
denature& ENZYME CONCENTRATION denature
o At a d
substrate concentration of A, the enzyme
d
activity increases as substrate concentration
increases up till a point X. pH
o A further increase in substrate concentration does
not increase the rate any further because all the
enzyme molecules are used up (saturated). Enzyme
concentration is the limiting factor.
o The only way to increase enzyme activity is to
increase the enzyme concentration to B.
o But when it reaches point Y again, enzyme
concentration becomes the limiting factor again.
Enzyme
activity
Y
Enzyme
concentration
B
Enzyme
concentration
A
X
LIMITING FACTOR
Substrate
concentration 5
CHAPTER 4: ENZYMES
o Any factor that directly affects the rate of a
chemical reaction when its quantity is changed is
called a limiting factor.
o The value of the limiting factor must be
increased so as to increase the rate of
reaction.
COENZYMES
o Some enzymes require another compound called a
coenzyme to be bound to them before they can
catalyse reactions.
o Coenzymes are non-protein organic compounds,
most are vitamins (B complex vitamins are essential
components of many coenzymes)