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6129 Proteomics Praticals
6129 Proteomics Praticals
Proteomics
Practicals
Submitted to
Dr.irfan manzoor
Submitted by:
M Mohsin
Roll no:
(6129)
Department of Biochemistry
Government College University Faisalabad
Practical no: 1
How to download genome
“The genome is the entire set of DNA instructions found in a cell”
In humans, the genome consists of 23 pairs of chromosomes located in the cell's nucleus, as well
as a small chromosome in the cell's mitochondria. A genome contains all the information needed
for an individual to develop and function.
7. Click on Refsek.
10. Click on gen bank full and create file.Your file will start downloading.
2) Search GeneMarks.
3) Click on GeneMark.
7) You can get of any plane DNA sequence by just pasting nucleotide sequence in GeneMarks.
Practical: 3
Translation of given DNA
Translate is a tool which allow translation of DNA sequence into protein sequence.here we will
use EXPASY translate tool.
2. Go to ExPASy tools.
7. You have got your protein sequence with open reading frames.
Practical: 4
Characterization of given protein
Protein characterization involves finding out amino acids that makeup the protein,also sequence
of amino acids and their numbers.It plays critical role in determining the safety and efficiency of
biological product.
2) Go to ExPASy tool.
3) Click on protoplasm tool.This tool is used for computation of various physical and chemical
parameters of given protein.
4) Copy open reading frame that is solved already and paste it in search box.
5) Click on compute parameters.
6) We can find amino acid sequence,their numbers, isoelectric point,melting point, boiling
point and many more information.
Procedure:
1. Open google chrome.
2. Search ExPASy.
3. Click on ExPASy.
2) Open NCBI.
6) Scroll down.
2. Search UNIPROT.
3. Click on UNIPROT.
6. You can click card or table depending on the form in which data is needed so I clicked table.
7. Scroll down.
8. Click on view proteins.