Shrimp

Mechanical dissociation
-Slicing
Seperated myoblast cell
-Cutting

Resuspended the myoblast

Shrimp tissue cell with the fresh medium
-DMEM solution
Enzymatic dissociation
-Trypsin -FACs test to seperated the myoblast
cell with the living cell
-Collagenase

Seperated cell

-Adding to the hemocytometer Myoblast cell cultured

and Straining with trypan blue
-Changing medium 2-3 days
solution
-Passaging the cell’s confluenece -Monitoring the confluence and
(Asssesment of cell viability)
the sign of contamination
-Trypsin add dtach the cell

Isolation the Living cell

Sub culturing