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Lab Training Exercises
Lab Training Exercises
Lab Training Exercises
Draw structural formulas and name (using both the Δ and ω nomenclatures) :
a) 18-carbon chain length fatty acid with two double bonds at ω6 and ω9 positions;
b) 20-carbon chain length fatty acid with three double bonds at Δ5, Δ8, and Δ11 positions.
c) Which of these fatty acids must be included in the diet and why?
3. Explain how carbons for fatty acid synthesis are transported from mitochondria into the cytosol.
Draw a schematic depiction of this process.
Cytosce
Mitochondria
e
↓
Citrate shuttle
kC < Citrate D
I
Oxalacetate
- -
Azety-CoA +
rate
L
Barty (OA
+ Oxal.
I p-citram
Lyase
↓ ---
Acety COA
·eavage
↓ ↓
Fatty Acids oxalantall
syn
ratty
.
Acids Syn
4. What is the preparatory reaction of fatty acid synthesis? Which enzyme catalyzes this
reaction? Briefly describe the catalytic activity(-es) of this enzyme and indicate coenzyme.
HCO3-QDMUCONCoA
Acet ye-CoA + ATP + +
ADP
ACC utilizes the coenzyme biotin to transfer a carboxyl group from bicarbonate to acetyl-
CoA, forming malonyl-CoA. This step is crucial for the regulation and initiation of fatty acid
synthesis.
5. What is the enzyme complex that catalyzes synthesis of palmitic acid? Briefly explain the
enzymatic activity(-ies) of this complex. Indicate coenzymes necessary for this complex
The enzyme complex responsible for synthesizing palmitic acid is fatty acid synthase (FAS).
FAS carries out a series of enzymatic activities, including acetyl and malonyl transfers,
condensation, reduction, dehydration, and enoyl reduction. These activities, repeated in cycles,
add two-carbon units to the growing fatty acid chain until palmitic acid is formed (C16:0). The
coenzymes involved are NADPH and the carrier protein ACP (Acyl Carrier Protein).