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CELL: THE UNIT OF LIFE

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CELL – Structural & Functional Unit of Life

DISCOVERY:-
1) Robert Hooke -> discovered a dead cell – CORK Cell
2) Anton Von Leeuwenhoek-> discovered first Living Cell.
Eg:- Bacterial Cell, RBC, Sperm.

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3) Robert Brown – discovered Nucleus inside the cell

CELL THEORY:
Botanist – Mathias Schleiden – plant cells – cell wall & plasma membrane
Zoologist – Theodore Schwann – Animal Cell – Plasma membrane
Together they formulated CELL THEORY
They failed to explain how cell divide?
Rudolf Virchow – Cell always comes from pre- existing Cell
Omnis Cellula – e – cellula.

# FINAL CELL THEORY:-


All living organisms are composed of cells/ products of cell.
All cells comes from Pre- existing cell.
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Smallest cell – Mycoplasma – 0.3 Um
Bacteria Cell :- 3-5 micro meter
Largest isolated Single Cell- Ostrich Egg
Human RBC diameter – 7 micro meter

PROKARYOTIC CELL:- FEATURES:- Membrane Less organelles


True nucleus Absent

> Bacterial Cell-


SHAPE EXAMPLES
1. Vibrio ( comma) Vibrio cholerae
2. Coccus (spherical) Streptococcus pneumonia
3. Spirillium ( spiral) Rhodospirillium
( helps in N2 Fixation)
4. Bacillus ( Rod) Bacillus thurengiensis
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1) Glycocalyx :- > Thick – capsule
> Thin – Slime
layer
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2) Cell Wall:- peptidoglycan ( Heteropolysaccharide)
|
N- Acetylmuramic acid N- Acetyl Glucosamine

> Protection
Mechanical support
Maintains the shape of cell

3) Plasma Membrane:- Lipid bilayer

4) FIMBRIAE:- made up of fimbrin protein , adhesion/ attachment to substratum

5) PILI:- Made up of pilin protein, for conjugation

6) INCLUSION BODIES:- Reserve bodies of food


Eg: Phosphate Granules, Cyanophycean granules, Glycogen.

7) CYTOPLASM:- arena of all metabolic activities, mostly made up of water, has


enzymes.
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8) MESOSOMES:- infoldings of plasma membrane.

9) NUCLEIOID:- Chromosomes, without histone proteins

10) PLASMID:- Extra chromosomal , ds circular DNA, Pathogenic,


Antibiotic resistant gene

11) RIBOSOME:- Protein factories, 70 S ( 30S smaller, 50S Larger)

12) FLAGELLA:- Locomotion, made up of Flagellin protein.

13) CHROMATOPHORES:- Coloured pigments – photosynthesis

14) VACUOLES: Contractile vacuole – osmoregulation – Amoeba


Gas Vacuole – Buoyancy – Blue Green Algae.

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PARAMETER PROKARYOTIC CELL EUKARYOTIC CELL
Size Small Large
Nucleus Well defined nucleus is Present
absent
Nucleolus absent present
Site of Translation Cytoplasm Cytoplasm, mitochondria +
plastids
Ribosome 70S only 70 s – Mitochondria +
plastids & 80 s Cytoplasm
Absent
Centriole absent Plant cell – absent
Animal cell- present
Plasmid present Absent
Glycocalyx Present Absent

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II) EUKARYOTIC CELL
A) CELL WALL:-

(ii) Fungal cell Wall:- Chitin (Homo polysaccharide), Polymer of


N- acetylglucosamine, it has Glycosidic Linkage

(iii) Algal Cell Wall:- Cellulose + Galactose + Mannans + Minerals (CaCo3)


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FUNCTIONS OF CELL WALL:-
1) Shape of the cell
1) Allows minerals to pass in & out of cell
2) Protects cell from mechanical injury
3) Protects against bacterial, fungal , viral
infections

B) PLASMA MEMBRANE/ CELL MEMBRANE:-


Singer & Nicholsan – 1972
RBC- A cell without organelles
Fluid Mosaic Model

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Plasma Membrane: 40% LIPIDS, 52% PROTEINS
|
Intrinsic Extrinsic
Eg. Receptor for Eg: Aquaporins–
hormone water
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FUNCTIONS:-
C) ENDO-MEMBRANE SYSTEM:- 1) Intercellular Junctions
> Organelles are connected 2) Secretion ( Exocytosis)
3) Cell Division
structurally & Functionally 4) Movement of Ions
Actively – ATP Reqired
> ER, Golgi, Lysosome, Vacuole Passively – No ATP
5) Cell Growth
6) Endocytosis - engulfing
7) Osmosis

Fluidity:-
Lateral Movement- same layer PROTEIN
> Flip Flop Movement – Between layer LIPIDS

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ENDOPLASMIC RETICULUM (ER)
(i) Rough ER:
Continuous with outer membrane Nucleus.
Protein synthesis & secretion

(ii) Smooth ER:-


No Ribosome
Appears Smooth
Muscle cell – sarcoplasmic reticulum
Functions:-
> Synthesis of lipids & steroids
Detox in liver
Store ca+ in muscles

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GOLGI APPARATUS:-
Discovery: 1898, Camillo Golgi
Plant Cell – dictyosomes
FUNCTIONS:
Modified into acrosome in sperm
Glycosylation – addition of carbohydrate
chain
Formation of PM during cytokinesis
Process + Packing + Transport

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LYSOSOMES:-
Spherical sac like structure
Formed by packaging in golgi
Also called Suicidal Bags
Single membraned
Hydrolytic Enzymes ( lipase, amylase, protease)
Acidic (PH – 4 TO 5)

VACUOLES:-
Membrane – TONOPLAST
Contains- water + sap+ Excretory waste ( SAP Vacuole)

PLANT CELL:
Maintain Turgidity
Opening & closing of stomata
Vacuole- 90% Space
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1. LEUCOPLAST: Colourless Plastids
PLASTIDS:- Present near nucleus
All Plant Cells and some euglenoids Storage
Granum id Absent
Eg: Amyloplast – stores carbohydrates – Potato
tubers, rice.
Elaioplast – Stores fat 7 oils – castor oil
Aleuroplast- Store proteins – Aleurone cell of
maize

2. CHROMOPLAST:- Pigment containing plastid.


Carotenoids- carrot+ papaya
It is a precursor of Vit A- Rhodopsin- EYES
DEFICIENCY- Night blindness
TOMATO:- Green to reddish
(Chloroplast)……….(Chromoplast)
(i) Xanthophyll
(ii) Lycopene
> CHILLI:- Green to Red
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NOTE: Semi autonomous Organelles are
MITOCHONDRIA & CHLOROPLAST
Endosymbiont Theory
Own DNA
ETS + ATP Synthesis

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RIBOSOME:- >Bacterial Ribosome/
Discovery- 1953, George Pallade Mitochondrial/ Chloroplast

Structure: SMALLER: 30S


LARGER: 50S
= 70S
Where S is Svdberg unit/
Sedimentation coefficients

Eukaryotic Ribosome has


Smaller 40S 7 LARGER 60S = 80S

FUNCTIONS: Polysome
> When many ribosome are
attached to same mRNA

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CYTOSKELETON:
> Made up of extremely minute proteinaceous
fibres
Occurs only in eukaryotic cell
Function – support, motility, maintenance of
shape

TYPES:-
MICROTUBULES: Hollow, unbranched cylinder
of 13 parallel protofilaments
made up of Tubulin Protein
MICROFILAMENTS: Solid, unbranched rod like
filaments made up
of Globular Proteins
INTERMEDIATE FILAMENTS: Helps in packing
of chromatin
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CENTROSOME & CENTRIOLE
Non- Membrane bound organelle
Two centrioles arranged perpendicular to
eachother
Animal cell + Fungi+ Algal cell
Absent in plant cell

STRUCTURE OF CENTRIOLE /
CART – WHEEL STRUCTURE

Functions:-
Basal body – cilia, Flagella
Also forms spindle fibre during
cell division
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CILIA:
Shorter
Only eukaryotic cell
Particle movement in
specific directions

FLAGELLA:
Longer
Prokaryote & Eukaryotic
Cell Movement

( 9+ 2) arrangement

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NUCLEUS:-
>First described by Robert Brown
>Stained with basic dye by Flemming

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MICROBODIES:-

Peroxisomes:- Participate in photorespiration ( C2 Cycle)


Catalase Enzyme
Glyoxysomes:- Glycolate Cycle Enzymes
Fat & Carb Cycle

PEROXISOME
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----------------Chapter END----------------

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