DANI Instruments

a scent of future

International Sales & Service Meeting 2007

 AS
Automatic sampler
 AS
Automatic sampler

is an automatic sampler to inject

liquid samples in DANI GC
 Sample trays and
syringe needles are
positioned away from
all heated surfaces.
This configuration
prevents volatile
solvent evaporation
and degradation of
thermally sensitive
samples.

MASTER AS – Sample Integrity

 Why an Automatic Sampler?

The automatic sampler assures:

– identical movements
– constant amount of solvent, air, internal
standard and sample into the syringe
– constant speed of the plunger and time
intervals

MASTER AS – Why an Automatic Sampler

It offers repeatability and accuracy not available
by manual injection.
By manual injection, RSD% is never lower than
3%, ever with cold injection.
automatic sampling manual sampling

3,5

2,5

1,5

0,5

0
C12 C16 C20 C24 C26 C32 C36 C40

MASTER AS – Why an Automatic Sampler

Providing innovative sampling solutions, the
DANI AS maximizes
laboratory productivity, mixing robotic
technology with advanced three-dimensional
space movements (X-Y-Z)

MASTER AS – Innovation
3D Space Movements

X Z

MASTER AS – 3D Space Movements

 Sample Vial Tray

AS provides unprecedented
automation and greater flexibility in sample
capacity.

It provides maximum sample throughput with a

standard 160 sample tray for 2mL vials or 65
sample tray for 10mL vials.

MASTER AS – Sample Vial Trey

 Syringe

It is possible to use syringes of several volumes

5, 10, 25, 50, 100, 250, 500 μL

Sample capacity (2 ml vials) 160

Sample capacity (10 ml vials) 65

Solvent vials 5

Waste vials 5

Syringe sizes accepted 5, 10, 25, 50, 100, 250, 500 µl

MASTER AS – Syringe
The sample volumes, from 0.1
to 500 µl, are measured with
great accuracy and precision.
This guarantees repeatability
and linearity of the quantitative
data.

MASTER AS - Precision
 Linearity of Syringes
600
y = 0,9762x
500 R2 = 0,9999

400
mg

300

200

100

0
0 100 200 300 400 500 600
ul

syringe type μL 10 50 100 500

max volume μL 9,5 48,75 98,85 487,86

 Gravimetric Test
Syringe Volume (µL) Weight (g) RSD%

10 1.007 0,173

25 0.887 0,157

50 0.893 0,158

100 0.905 0,157

250 0.862 0,199

500 0.970 0,153

Average of 8 samplings of 1 µL for each syringe

MASTER AS - Repeatability
 Chromatographic Test
C8 C10 C12 C14
1 188,749 157,419 102,825 101,723
2 188,317 156,319 102,568 101,577
3 187,259 155,916 101,847 101,07
4 187,479 156,438 101,89 101,644
5 188,231 156,458 102,241 102,004
6 188,108 156,314 102,214 101,828
7 188,109 156,861 102,165 101,956
8 188,024 156,89 101,859 101,196
9 188,73 156,865 102,498 101,875
10 188,891 156,833 102,431 101,771
11 188,518 157,469 102,749 101,401
Media (mV.s) 188,220 156,707 102,299 101,640
SD 0,512 0,474 0,347 0,305
RSD% 0,272 0,303 0,339 0,300
 Linearity of 500μL Syringe

600

500 y = 0,9733x
R2 = 0,9999

400
mg

300

200

100

0
0 100 200 300 400 500 600
ul

MASTER AS - Linearity
 Configuration

Software

Touch Screen

MASTER AS – Configuration
Vial
&
Syringe

MASTER AS – Setup
Injection Mode

4 parameters
• Solvent plug
• Internal standard
• Sample
• Air plug

MASTER AS – Parameters
Solvent Plug

With solvent plug

it is not possible to
rinse with sample
and to set a
number for
plunger strokes

MASTER AS – Injection Mode

Internal
Standard
This parameter is
an innovation of
master AS
compared with
old ALS 1000

MASTER AS – Injection Mode

Sample
Handling

MASTER AS – Parameters
Solvent
&
Waste

It is possible to set
up to five solvents
and I.S. with five
wastes, to
eliminate the
possibility of
sample carry over

MASTER AS – Setup
Solvent
Wash
All, any or a
combination of pre
and post solvent
washes can be
programmed during
method setup

MASTER AS – Parameters
Injector

Up to three
injectors can be
selected for each
method

MASTER AS – Parameters
Sample
Sequence

You can set up to

25 sequences of
methods

MASTER AS – Sequence
Priority
Sequence

The possibility
during a analysis
of setting up a
sequence that
starts
immediately after
the end of the
current run

MASTER AS – Sequence
Software Setup

MASTER AS – Setup
Software Setup

MASTER AS – Parameters
 Technical Specifications

System Type X-Y-Z Robot

Sample Capacity (2ml vials) 160
Sample Capacity (10ml vials) 65

Solvent / Waste Vials 5/5

Minimum Sample Injection 0.1 µl
Maximum Sample Injection 500 µl
Syringe sizes 5, 10, 25, 50, 100, 250, 500 µl

Sampling Mode Customisable

Flush&Dry Sampling Mode YES
Injection per Vial up to 100
Sample pre-injection washings 25 times/25 sec
Solvent post-injection washings 25 times/25 sec
Variable Sampling Depth YES

MASTER AS - Specifications
Variable Injection Speed YES
Internal Standard Addition YES
Priority Sequence YES
Injection Positions 3
Repeatability RSD% < 0.5% (C12-C40)

Instrument Control Touch Screen on MASTER GC

Clarity Software
External Software Control YES
Data Communications RS-232
Dimensions (WxHxD) 455x750x650 mm
Weight 13Kg
Power Supply 85-264Vac 50-60 Hz

MASTER AS - Specifications
What do you need for your
AS ?

MASTER AS - Consumables
 Vials
VIALS for Liquid Autosamplers
Description Set of Code
Vial 12x32 mm - volume 100 µl 50 pieces 1262.011 004 MASTER AS - ALS1000 - HT300A - HT310A
Vial 12x32 mm - volume 100 µl 50 pezzi Sample
Vial 12x32 mm - volume 1,5 ml 50 pieces 1262.095 002 MASTER AS - ALS1000 - HT300A - HT310A
Vial 12x32 mm - volume 1,5 ml 50 pezzi Sample
Vial 23x50 mm - volume 10.5 ml - screw top + cap + septa TFE/SIL 5 pieces 1262.017 001 MASTER AS - ALS1000
Vial 23x50 mm - volume 10.5 ml - chiusura a vite completo di tappo e setto TFE/SIL 5 pezzi Solvent
Vial 23x50 mm - volume 10.5 ml - screw top + cap + septa TFE/SIL 5 pieces 1262.017 001 MASTER AS
Vial 23x50 mm - volume 10.5 ml - chiusura a vite completo di tappo e setto TFE/SIL 5 pezzi Waste

MASTER AS - Consumables
 Septa and Crimps
Septa and Crimp for Liquid Autosamplers
Description Set of Code
Crimp seal for Vial 12x32 mm 100 pieces 1260.895 001 MASTER AS - ALS1000 - HT300A - HT310A
Capsula per Vial 12x32 mm 100 pezzi
Septa TFE/BUT for Crimp seal 100 pieces 1260.995 004 MASTER AS - ALS1000 - HT300A - HT310A
Setto TFE/BUT per Capsula 100 pezzi
Septa TFE/SIL for Crimp seal 100 pieces 1260.905 004 MASTER AS - ALS1000 - HT300A - HT310A
Setto TFE/SIL per Capsula 100 pezzi
Hand crimper for Crimp seal 1 piece 1343.000 006 MASTER AS - ALS1000 - HT300A - HT310A
Pinza Chiudi Vials per Capsula 1 pezzo

1343.000 006

1260.995 004

1260.895 001

MASTER AS - Consumables
 Syringes

SYRINGES for Liquid Autosamplers

Description Set of Code
Microsyringe 5 ul 1 piece 1250.400 003 MASTER AS
Microsiringa 5 ul 1 pezzo
Microsyringe 10 ul 1 piece 1250.401 002 MASTER AS - ALS1000
Microsiringa 10 ul 1 pezzo
Microsyringe 25 ul 1 piece 1250.400 004 MASTER AS - HT300A - HT310A
Microsiringa 25 ul 1 pezzo
Microsyringe 50 ul 1 piece 1250.400 005 MASTER AS - HT300A - HT310A
Microsiringa 50 ul 1 pezzo
Microsyringe 100 ul 1 piece 1250.400 006 MASTER AS - HT300A - HT310A
Microsiringa 100 ul 1 pezzo
Microsyringe 250 ul 1 piece 1250.400 007 MASTER AS
Microsiringa 250 ul 1 pezzo
Microsyringe 500 ul 1 piece 1250.400 008 MASTER AS
Microsiringa 500 ul 1 pezzo

MASTER AS - Consumables
 Conclusions

Combined with GC
it offers a complete system,
versatyle and reliable for
every injection type.

MASTER AS – Conclusions