(21223)(Micro.

)- I
Sem.
M.SC.
NS-3167

M.Sc. (Microbiology)
Examination, Dec.-2023
Iastrumentation and Microbial
Techniques
(GM-101)
M.Sc. (Micro.)
7ime : 1hree Hours / |Maximum Marks : 50

Note : Attempt questions from all sections

as per instructions.
Section- A
(Very Short Answer Questions)
Note : Attempt allquestions. Each question
carries 2markS. Very short answer is

required not exceeding 75 words. OPPO

/A55

2x5=10
P.T.O.
 . Write notes on the following:
(a) Chemical methods of sterilization
(b) Evaluation of antimicrobial

effectiveness.
12. Discuss the principle, procedure and
applications of high performance liquid
chromatography?
13. Explain in detail about the principle and
applications of ultracentrifugation and
OPPO
A53
Iyophilization.
 E1. Define simple and differential staining
PPOA55
techniques and give examples of each
staining technique?
2.
Deine simple and complex media with
two examples of each media?
3. Discuss about the filtration method of
sterilization?
4. Write down the principle of basic
spectrophotometry?
5. Discuss about X-ray Diffraction?
Section-B

(Short Answer Questions)

Note : Attempt any two questions. Each
question carries 5 marks. Short

answer is required not exceeding 200

Words. 2x5=10

6. Write down the principle and procedure

of Acid fast staining?
NS-3167/2
 culture
7.
Enlist the pure

plate miethod
techniques
of
and
pour
discuss
inoculation?
Describe the principle and applications of
8.

electrophoresis for protein separation?

Section-C

(Detailed Answer Questions)

Note : Attempt any three questions. Each

question carries 10 marks. 10x 3=30

9. Describe the principle and draw a well

labeled diagram cf light micrOScope?

Differentiate between light microscopy

and electron microscopy?

media and common

10. Define culture

culture media? Discuss

ingredients of

type of culture media?

about the
P.T.O.
NS-3167 /3