1.

Diseña los oligos necesarios con el sistema de Janke et al 2004, para delecionar el gen MEC1
con resistencia a Nat.

Indica el nombre del plásmido y los oligos forward (S1 o S3) y reverse (S2 o S4)

>YBR136W_S288C MEC1 SGD:S000000340 ORF Genome integrity checkpoint protein and PI

kinase superfamily member

1 TACCCGCCGA CGGACAATTT TGAAGAGAGA TGATTAATGA AGACAAAGTG AGGCTGGACA

61 ACAAGAACGA CATACACCGC GTAAAGGCCC ACAAGACTGC ATGGAATCAC ACGTCAAATA

121 TCTTGACGAA TTGATATTGG CAATAAAAGA CCTGAACTCG GGGGTGGATT CAAAGGTGCA

181 GATTAAAAAA GTGCCCACGG ATCCATCTTC TTCTCAGGAG TACGCCAAGA GTTTAAAGAT

241 CCTGAACACC CTCATAAGAA ACCTAAAAGA TCAAAGAAGG AACAATATCA TGAAAAATGA

301 TACTATATTT TCGAAAACAG TTTCCGCCCT TGCCTTATTG TTGGAGTACA ACCCCTTCTT

361 GCTTGTTATG AAGGATTCCA ACGGGAACTT TGAGATACAA AGGCTGATAG ATGATTTCCT

421 CAACATATCC GTTCTGAACT ATGATAATTA CCACAGAATA TGGTTTATGA GGCGAAAATT

481 AGGCAGCTGG TGCAAAGCAT GTGTCGAATT TTACGGAAAA CCTGCTAAGT TTCAGCTTAC

541 TGCACATTTT GAGAACACCA TGAATCTTTA CGAACAGGCC TTGACTGAAG TCTTGTTGGG

601 CAAGACTGAG CTTCTCAAAT TTTATGACAC CTTGAAGGGT CTATACATTC TTTTATACTG

661 GTTCACTTCG GAGTATAGTA CTTTTGGGAA CTCTATAGCA TTCTTAGATT CTTCTTTGGG

721 GTTCACGAAA TTTGACTTTA ACTTCCAACG ATTAATCAGG ATTGTTCTTT ACGTCTTTGA

781 TTCCTGCGAA CTAGCAGCAC TAGAATATGC CGAAATCCAA CTCAAATATA TTTCTCTAGT

841 TGTGGACTAT GTTTGCAATA GAACAATTTC CACAGCCCTG GATGCCCCAG CGTTAGTTTG

901 TTGTGAACAA TTAAAGTTTG TATTGACTAC TATGCATCAT TTTTTGGATA ACAAGTATGG

961 GCTCTTGGAT AATGACCCCA CTATGGCCAA AGGAATTCTT CGACTATATT CTCTTTGCAT

1021 TTCTAACGAT TTCTCAAAAT GCTTTGTAGA CCACTTCCCA ATTGACCAGT GGGCAGATTT

1081 TTCACAAAGT GAACATTTTC CGTTCACGCA GTTGACTAAT AAAGCTCTCT CGATTGTATA

1141 TTTTGATTTG AAAAGAAGGT CCCTACCTGT TGAAGCTTTA AAGTACGATA ATAAGTTCAA

1201 CATCTGGGTA TACCAATCGG AGCCGGACTC GAGCTTGAAA AATGTCACTT CTCCCTTTGA

1261 TGATCGATAT AAGCAGCTGG AAAAGCTAAG GTTGCTAGTA CTAAAGAAGT TTAACAAGAC

1321 AGAAAGAGGA ACTTTGCTCA AATACCGCGT GAACCAGCTA AGTCCTGGAT TTTTTCAAAG

1381 AGCTGGAAAC GATTTCAAGC TAATTTTAAA TGAAGCATCT GTATCCATTC AAACTTGTTT

1441 CAAGACAAAC AATATAACAA GGCTAACATC ATGGACTGTA ATTCTCGGAC GTCTAGCCTG

1501 TCTAGAATCA GAGAAGTTTT CCGGCACTCT GCCAAATTCC ACAAAGGATA TGGATAATTG

1561 GTATGTTTGT CATTTATGCG ATATTGAGAA AACTGGCAAC CCTTTCGTGC GAATAAATCC

1621 AAATAGACCA GAGGCTGCGG GTAAATCAGA AATCTTCAGG ATACTTCATT CAAACTTTCT

1681 ATCTCACCCA AATATAGATG AATTTAGCGA ATCTTTGTTA AGTGGCATCT TATTTTCTCT

1741 ACATAGGATA TTTTCACACT TTCAACCTCC AAAACTTACA GATGGAAACG GTCAAATCAA

1801 TAAGAGCTTT AAACTGGTAC AAAAGTGCTT TATGAATTCT AACAGATACC TACGTTTATT

1861 AAGTACTAGA ATTATACCTT TATTCAATAT ATCAGACTCT CATAATTCCG AAGATGAACA

1921 CACTGCCACG CTGATAAAGT TTCTACAATC TCAAAAATTG CCAGTGGTGA AAGAAAATTT

1981 AGTCATTGCT TGGACACAAT TAACATTGAC GACTTCTAAT GATGTATTTG ATACACTACT

2041 TTTGAAACTG ATTGATATTT TCAATTCTGA TGATTATAGT TTACGAATAA TGATGACTTT

2101 GCAAATTAAA AATATGGCCA AAATTTTAAA GAAAACACCA TATCAATTAC TATCGCCTAT

2161 TTTACCTGTA TTACTAAGAC AGTTGGGAAA AAACCTCGTG GAAAGAAAAG TTGGCTTTCA

2221 AAATTTAATA GAATTATTGG GATATTCTTC AAAAACAATT CTCGATATTT TCCAGAGATA

2281 TATCATCCCT TATGCAATTA TTCAATATAA GAGCGATGTG CTAAGTGAAA TTGCTAAGAT

2341 TATGTGTGAT GGCGATACAA GTTTAATTAA CCAAATGAAG GTTAATTTAC TGAAAAAAAA

2401 CAGTAGGCAA ATATTTGCCG TAGCTTTGGT AAAACACGGA TTATTTTCTT TGGATATCTT

2461 GGAAACCCTT TTTTTAAATA GGGCTCCAAC TTTTGACAAA GGATATATAA CTGCATACCT

2521 TCCCGATTAT AAAACTTTAG CTGAAATAAC GAAGCTCTAC AAAAACAGCG TTACTAAAGA

2581 TGCAAGTGAC AGCGAGAATG CTAATATGAT TTTATGCTCT TTGCGATTTT TAATCACCAA

2641 TTTTGAAAAA GACAAAAGGC ATGGTTCGAA GTACAAAAAT ATCAATAACT GGACGGATGA

2701 TCAGGAACAA GCGTTCCAAA AGAAACTACA GGATAATATC TTAGGTATTT TCCAAGTTTT

2761 TTCGAGTGAC ATACATGATG TTGAAGGCCG CACCACTTAC TACGAAAAGT TAAGGGTTAT

2821 CAATGGCATT TCTTTTCTTA TCATATATGC CCCCAAAAAA TCAATAATTT CCGCATTAGC

2881 CCAGATTAGT ATTTGTTTGC AAACAGGACT TGGGCTTAAG GAAGTTCGAT ACGAGGCCTT

2941 TAGATGTTGG CATCTGTTAG TTCGCCATCT AAATGATGAA GAACTCTCTA CTGTTATAGA

3001 TAGCTTAATT GCATTCATAC TTCAAAAGTG GTCTGAGTTC AACGGAAAAC TTCGAAATAT

3061 AGTGTACAGT ATACTGGATA CCTTAATCAA AGAGAAATCG GACCTGATTT TGAAATTAAA

3121 ACCTTACACT ACTTTGGCTT TAGTAGGCAA GCCTGAATTA GGTATTTTAG CTCGTGATGG

3181 CCAATTTGCA AGAATGGTGA ATAAAATAAG AAGTACCACG GACCTTATAC CCATATTTGC

3241 TAATAACTTG AAAAGTAGTA ACAAGTATGT CATAAACCAA AATTTAGACG ATATAGAGGT

3301 ATATCTTCGG AGAAAGCAGA CAGAAAGATC GATTGATTTT ACACCAAAGA AGGTTGGGCA

3361 AACTTCTGAT ATAACATTAG TTTTGGGTGC TTTATTAGAC ACTTCTCATA AGTTTAGAAA

3421 TTTAGACAAG GACCTATGCG AGAAGTGCGC CAAATGTATC AGTATGATTG GTGTTTTAGA

3481 CGTTACAAAG CATGAGTTTA AAAGAACAAC ATATTCAGAA AACGAAGTTT ATGATTTGAA

3541 TGATAGTGTT CAAACTATTA AGTTCTTGAT ATGGGTCATA AATGATATCC TCGTTCCTGC

3601 GTTTTGGCAA AGTGAGAATC CCAGCAAGCA ATTGTTTGTT GCCCTTGTCA TACAGGAATC

3661 ATTAAAATAT TGCGGGCTAA GTTCAGAGTC ATGGGATATG AACCATAAAG AATTATATCC

3721 AAATGAAGCC AAACTATGGG AAAAGTTTAA CTCTGTCTCC AAGACAACCA TCTATCCGCT

3781 TTTATCTTCC TTGTATCTTG CGCAATCATG GAAAGAATAT GTCCCGCTAA AATATCCTTC

3841 TAATAACTTC AAGGAAGGAT ACAAAATTTG GGTGAAAAGG TTTACATTGG ATTTATTGAA

3901 AACAGGTACA ACAGAAAATC ATCCATTACA CGTGTTTTCC TCTTTGATTA GGGAAGATGA

3961 TGGCTCACTA TCAAATTTTT TGCTACCTTA TATTTCTCTG GACATTATTA TCAAGGCAGA

4021 AAAAGGAACT CCATACGCTG ATATTTTAAA CGGGATTATT ATTGAATTTG ACAGCATTTT

4081 CACGTGCAAT CTGGAAGGAA TGAATAACTT GCAAGTGGAT TCGTTAAGAA TGTGCTATGA

4141 ATCCATCTTC AGAGTTTTCG AATATTGCAA AAAATGGGCA ACTGAGTTTA AACAAAATTA

4201 CAGTAAACTA CACGGCACTT TTATCATTAA AGATACGAAA ACAACTAACA TGCTTTTGAG

4261 AATAGATGAG TTTTTGCGAA CAACCCCTTC AGATTTGCTA GCTCAACGCT CCTTAGAGAC

4321 GGATTCTTTT GAAAGGTCTG CTCTATACCT TGAACAGTGC TATCGACAGA ATCCTCACGA

4381 TAAGAACCAA AATGGACAAC TACTGAAAAA TTTACAAATC ACATACGAAG AAATAGGAGA

4441 CATTGACTCA CTCGATGGTG TACTGAGAAC CTTTGCTACA GGAAACTTGG TTTCTAAAAT

4501 TGAAGAATTG CAATATTCTG AAAACTGGAA ACTCGCACAA GACTGCTTTA ATGTCCTCGG

4561 CAAATTTTCA GATGACCCCA AAACTACAAC CAGGATGCTA AAGTCTATGT ATGACCACCA

4621 ATTGTATTCT CAAATAATAT CGAACTCTTC GTTCCATTCT TCAGACGGAA AAATTTCTTT

4681 GTCTCCAGAT GTGAAGGAAT GGTACAGCAT AGGTCTTGAA GCTGCAAATC TAGAAGGCAA

4741 TGTTCAAACT TTGAAAAATT GGGTAGAACA AATAGAGAGT TTAAGAAATA TTGACGATAG

4801 AGAAGTACTT TTGCAGTACA ATATTGCGAA AGCTTTAATT GCCATCTCAA ACGAGGATCC

4861 ATTAAGGACT CAAAAATACA TCCACAATTC CTTTAGGCTT ATCGGAACAA ATTTTATAAC

4921 GTCATCTAAA GAGACGACGC TGCTAAAGAA ACAGAATTTA TTGATGAAAT TACACAGTTT

4981 ATATGACCTC AGTTTTTTAT CTTCTGCGAA AGATAAGTTT GAATACAAAA GTAACACTAC

5041 CATACTCGAT TATCGAATGG AACGTATTGG GGCTGACTTC GTGCCAAATC ATTACATATT

5101 GTCAATGAGA AAGTCATTTG ACCAATTGAA AATGAATGAA CAAGCAGACG CTGACTTAGG

5161 AAAAACATTC TTCACTTTAG CCCAATTGGC GAGAAACAAC GCTAGGCTAG ATATAGCCTC

5221 CGAATCATTA ATGCATTGTT TGGAAAGGCG GTTGCCTCAG GCAGAGTTGG AGTTTGCTGA

5281 AATACTATGG AAGCAAGGTG AGAATGATAG AGCCTTAAAG ATAGTGCAAG AAATACATGA

5341 AAAGTATCAA GAAAATTCCT CGGTTAATGC TCGCGATCGT GCCGCCGTGC TATTAAAGTT

5401 TACTGAATGG TTAGACCTTT CGAACAATTC AGCGTCCGAA CAAATTATTA AACAATATCA

5461 GGATATTTTT CAGATTGATT CTAAATGGGA TAAACCATAT TACTCTATTG GCTTATACTA

5521 TAGTAGACTA CTTGAGCGCA AAAAAGCAGA GGGTTATATT ACTAATGGTC GTTTTGAGTA

5581 CAGGGCAATA TCTTACTTTT TATTGGCATT TGAAAAGAAC ACTGCTAAAG TAAGAGAAAA

5641 TTTGCCCAAA GTTATCACGT TTTGGCTAGA TATTGCGGCC GCATCAATTT CTGAAGCTCC

5701 TGGAAACAGA AAGGAAATGC TGAGTAAGGC TACGGAAGAT ATATGTAGTC ATGTTGAAGA

5761 AGCGCTGCAG CATTGTCCCA CTTATATTTG GTACTTTGTT TTGACTCAGT TGTTATCTAG

5821 GTTATTACAT TCTCATCAAT CATCGGCCCA GATAATAATG CACATACTGC TAAGTTTGGC

5881 TGTTGAATAC CCCTCTCATA TTTTATGGTA TATCACAGCC CTTGTAAATT CCAATTCTTC

5941 AAAAAGAGTT CTTCGTGGTA AGCATATTTT AGAAAAGTAT AGACAACATT CGCAAAATCC

6001 TCATGATCTA GTTTCTAGTG CATTGGATTT AACGAAAGCA TTAACTCGTG TCTGTTTGCA

6061 AGATGTCAAA AGCATTACAA GTAGATCAGG CAAATCTTTA GAAAAAGACT TCAAATTTGA

6121 CATGAACGTG GCCCCATCTG CAATGGTTGT TCCAGTAAGA AAAAATTTAG ACATCATTTC

6181 ACCACTAGAG TCTAACTCAA TGAGGGGCTA TCAACCATTT AGGCCGGTTG TTTCTATAAT

6241 TAGATTCGGA TCATCTTATA AAGTGTTTTC TTCATTAAAG AAGCCAAAAC AATTGAACAT

6301 AATAGGTTCA GATGGCAACA TTTATGGGAT CATGTGTAAG AAGGAAGATG TCCGACAAGA

6361 TAACCAATAT ATGCAGTTCG CCACAACAAT GGATTTTCTT CTGAGTAAGG ACATAGCTTC

6421 AAGAAAAAGA AGCCTGGGCA TAAATATTTA CTCCGTACTA TCTCTTCGAG AAGACTGTGG

6481 GATATTGGAA ATGGTACCGA ATGTTGTAAC TTTAAGATCT ATTCTTTCTA CAAAGTACGA

6541 AAGTCTGAAA ATTAAGTATA GCCTGAAAAG TCTACATGAT AGGTGGCAGC ACACCGCAGT

6601 AGATGGAAAA CTCGAGTTTT ACATGGAACA GGTAGATAAA TTTCCTCCAA TCTTGTACCA

6661 ATGGTTTTTA GAAAACTTTC CTGATCCAAT CAATTGGTTC AACGCCAGGA ATACGTATGC

6721 CAGATCTTAC GCCGTCATGG CAATGGTTGG CCATATATTA GGTCTAGGTG ATAGGCACTG

6781 TGAAAACATA TTACTAGATA TACAGACGGG TAAAGTTCTT CATGTAGACT TCGACTGTTT

6841 ATTTGAGAAA GGCAAAAGGT TACCTGTCCC AGAAATTGTT CCCTTCAGAC TAACACCAAA

6901 TTTATTGGAT GCGTTGGGCA TAATTGGGAC AGAAGGAACA TTTAAGAAGT CTAGTGAAGT

6961 CACGTTGGCT TTAATGAGAA AAAATGAAGT AGCGTTGATG AATGTGATCG AAACAATTAT

7021 GTACGATAGA AACATGGACC ACTCAATTCA AAAAGCGCTA AAGGTCTTAA GAAACAAAAT

7081 CCGCGGTATA GATCCGCAGG ATGGCCTGGT ATTGAGTGTT GCTGGCCAAA CAGAAACATT

7141 GATCCAAGAA GCAACATCAG AAGACAATCT AAGCAAGATG TATATTGGTT GGCTTCCATT

7201 TTGGTAACGA CTTTCCACCA TTTTCGGCAA CAGACGAACT TCCTCTTGAT CTAACCATCA

7261 CTGCAGGTGC TTTTCTCCGG CGGAGTTAAT AGATACTTAT CCCCGCT

1. Diseña los oligos necesarios con el sistema de Janke et al 2004, para etiquetar el gen HAT1
en Ct con el Tag 9MYC y auxotrofía HIS3.

Indica el nombre del plásmido y los oligos forward (S1 o S3) y reverse (S2 o S4)

>YLL022C_S288C HIF1 SGD:S000003945 ORF Non-essential component of the HAT-B histone

acetyltransferase complex

1 TGCACTGAAG TTTCGCGTTT TTTCCAATTT TGTATTATTT CATTCTACTC AATTTTGAGG

61 TAACATCGGC GATGAGGTCT CCTTCTAGAA GTAACAGAAT ATGAAACTAA GGGCAGAAGA

121 CGTGTTGGCA AACGGAACTA GCAGACATAA AGTTCAAATT GATATGGAAA GACAGGTTCA

181 AATTGCAAAA GATTTACTCG CACAGAAAAA GTTCTTAGAA GCTGCTAAAC GCTGCCAACA

241 AACTTTGGAC TCTCTCCCAA AAGATGGTTT GTTGCCTGAT CCTGAACTGT TTACTATATT

301 CGCACAAGCT GTATACAACA TGGAAGTACA GAATTCTGGT AATCTGTTTG GGGACGCTCT

361 TCTGGCAGGT GACGATGGAT CTGGGTCTGA GTCTGAGTCT GAGCCTGAGT CTGATGTGAG

421 CAATGGAGAA GAGGGGAACG AGAACGGTCA AACAGAAATT CCTAATTCTA GGATGTTCCA

481 ATTCGACCAA GAAGAGGAAG ACTTGACTGG TGATGTCGAC AGTGGTGATA GTGAGGACAG

541 TGGCGAAGGT AGCGAAGAAG AGGAAGAAAA CGTGGAGAAA GAGGAAGAAC GCTTAGCTTT

601 GCACGAATTG GCTAACTTCA GTCCAGCGAA TGAACATGAC GATGAAATTG AAGACGTATC

661 GCAACTTCGC AAGTCTGGTT TCCACATTTA CTTTGAAAAT GATCTGTATG AGAATGCTTT

721 GGACCTACTG GCGCAGGCCT TGATGCTGTT GGGCCGCCCT ACAGCAGATG GCCAATCTCT

781 AACCGAGAAC AGCAGATTGC GCATCGGTGA TGTGTATATC CTGATGGGTG ACATCGAAAG

841 GGAGGCGGAG ATGTTCAGTA GAGCCATTCA TCATTACTTG AAGGCGCTTG GCTATTACAA

901 GACCTTGAAA CCCGCAGAAC AAGTAACTGA GAAGGTGATA CAAGCAGAAT TTTTGGTGTG

961 TGATGCTTTA AGGTGGGTTG ATCAGGTGCC GGCTAAGGAC AAACTAAAAA GATTCAAGCA

1021 TGCCAAGGCT CTGCTTGAAA AACACATGAC TACAAGACCC AAGGACAGCG AGTTACAGCA

1081 GGCAAGGCTC GCACAGATTC AAGACGATAT TGATGAGGTG CAAGAAAATC AGCAGCACGG

1141 CTCCAAGAGG CCTCTTTCGC AGCCCACGAC CTCCATCGGC TTCCCCGCCC TCGAAAAGCC

1201 CCTTGGTGAC TTCAATGATC TCTCTCAACT GGTCAAGAAG AAGCCTAGAA GGCATTGATA

1261 GTTTTGTGTA AAATGTAGCA ACCTACATAC GTAATGTATA TTTCTCCTTC TTTGGTACGG

1321 CGAAAGGGCC CCATTTTAAA AGGGTGAAAA AATATTTA