Reg. No.

MANIP AL UNIVERSITY
FOURTH SEMESTER B.S. (ENGG.) & B.Sc. (APPLIED SCIENCES)
DEGREE EXAMINATION - MAY/JUNE 2015
SUBJECT: BIOCHEMISTRY (CH 241)
(BRANCH: BIOMEDICALIIND.BIOTECH/CHEMICAL)
Friday, May 29, 2015
Time: 10:00 - 13:00 Hrs. Max. Marks: 100

JlS Answer any FIVE full questions.

lA. What is mutarotation? Explain the important properties of monosaccharides .

. lB. Explain the practical application of reducing action of sugars in alkaline solution.
Differentiate between lactose and sucrose.
1C. Explain the chemistry and functions of proteoglycans.
(6+6+8 = 20 marks)

2A. Give the classification of fatty acids with suitable examples.

2B. Explain the structure, properties and esterification of cholesterol.
2C. Describe the different methods used in identification of fats and oils.
(6+6+8 = 20 marks)

3A. What are prions? Explain the denaturation of proteins and agents that cause denaturation.
3B. Classify the proteins on the basis of solubility and physical properties.
3C. Explain the structure oftRNA and its classes. Add a note on processing oftRNA.
(6+6+8 = 20 marks)

4A. Explain the role of mitochondria and cytosol in urea cycle.

4B. Explain the diagnostic applications of enzymes.
4C. What are plasmids? Explain the steps involved in genetic engineering.
(6+6+8 = 20 marks)

5A. Describe the reactions of glycolytic pathway.

5B. Write the composition of blood. Add a note on glycosuria and pentosuria.
5C. Explain the structure of haemoglobin and its properties.
(6+6+8 = 20 marks)

6A. Explain the gel filtration technique and its uses.

6B. Explain the role of carnitine in fatty acid metabolism. Explain the bioenergetics of beta
oxidation and its efficiency.
6C. List the major hormone secreting glands. Explain the peptide hormones with suitable
examples.
(6+6+8 = 20 marks)

7A. Give the classification of chromatographic techniques and its application.

7B. List the high energy compounds in biological systems and explain its functions.
_.J~ Explain the biological coupled reactions with suitable examples and its significance.
(6+6+8 = 20 marks)

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