INDE X

Sr. No. Date of Date of

Experiment Page Remarks
Experiment Submission
Seeedy of develormenlal
Skoges and lfecyele of TO
DrcosoPhela Am S oce O6
Ceelleare.

DevelOpmerta sloe
Of
V.S F 3aslrula O7

GaslruLa

TadrLe.

Deveeopmenlad
OF chick

o.m OF ChicK embryo

(8 howns )
wm of chte embyo
(aB hoeeny)
. m OP chiek embeyo
36 hous)
 Experiment Name / No:. Camlin Pago No. ol
Dato

AIM OF THE EXPERIMEnT

To sledg the lire cycle, of Drosophela From
Stock, Culture.

COLLECTION OF DROSOPHILA
Frruct. fFY 93 Cosmopoliton n destreubution The can be
eatsy collec ted by Keefcing e Cet rere FreurE Such ag
eava elc.

MATERIAL REQURED foR DROSOPHILA STUDIES -

d ocerent stocK of DeeyoPhela melanogasker.
Dro s9 hcla Sp Colleclion n bolley or tbe
|@)Aneesthetic etherc
tvEtherrizeng bottle Fclled cwcho platt Fenel ConeL haire
15 brush
Small food veres por. Cullog the Frue't Flies
morrgue bottees
lut asO ce Jlas stoerer or Collon pujged bollles.
Cce)SO CC lass Stopper erc Collon ujed bolles
oo cc 25o cC and S0oCC Bortosil Beaken.
wstocK, bollle or food Veals Aore seoroe oF the Feed.
Ctere botte orc bod vias (tesme.).
ziGlass Plale eorth c hete baek, drDP,
Ki) S o , boltle.
zi Stacn les Sled needles.
as3 auesus Soluten of s odiley hyPochlorwbe
Cv)BoD ncubator
Teacher's Signature:
Experiment Name /No: Camlin Pags No. O2.
Data

PROCE DURE
TaRe JSOC gla3s Storrer. close the moelh of
the bollle cth a cork . mote, o hole gn he Cork
thcocgh necK of khe lo Feenrel . pet ether Sooek
bollle .Collece frurk flyes n ballle end cloe
Corth elther Soared Colton ful fLye beeome
nacered The lies shocetd beanaesteked thoush
E lizht does of ancsthat'e eithe asin? a SAeciel
10 eberis bollle fled ith o Plesle fenel has
Smetl Caßlo Pl scuped n the neck, f the
fennel. Dresophila beaare ele cenderc sterreosscrd
mecrogope dufenent Spears of Cros Cfhila are
Sepanakd proees of mokag the feit plees
15 2ve ojain 9s Caleed ce-eitheri zabbn.

PRE PARATorN OF FEED MEDIUM FoR, FRUIT

FLIES !
Feed of khe étes es Prerarated by mexing deknike
Prerrabien Of the falaansnnetents.
Pa a lar loes baesl keep (Yo0 malimekens of
Gugro agar ajart Sml prc Penol aed end (o2a9
erendhing cupon the reuinemerk the feed anby'
Cen be ncreased by grerees? theJrad ents

Teacher's Signature
 nlactous

metamorc s3
yenet0gere
Srern
ferctetined

mutinucected
yoLk

mot
Dudobbo
Struled Embny
pupa
Feedot

- lire ccLe of Drosophila mekonagastoc

 Experiment Name / No.: Camlin Pago Na.
Dato

u mex the gradinest cweiL end store he Feed sn

a StocK botlle.
uPem the stocK ballle take feed erd eep sn
Rod vèral
ALTERNVATE MATHOD
|OBoElles oF Qome Cofcitg and test tebeobe
Cas ed ere skeriitoion of these boltle mey be
persrmed.
Arranf the follooingnjt onjredaent f Clkeune
medicem
Pololo -o0g
Ajcre
Propionic Aced-o.gmd.
yeast -O-S

PROCE DURE
-The Pototo oe becled , Peeled end mashed nto
a soft ReP.
o s homogenied cwzth waler, ofar ond dextrese
This mx tree s skentr zed crtb a beorer
coith thes mestK Cuered wth a clean oater
25 Jhike Pouper. kide wth eeither vutbere bGnd orc
peaseic. rreed
Teacher's Signature
 Camlin Pago No. Y
Experiment Name / No:
Dato

Seerilsed ot (SB presscere. or IS-20 minule n or

atocloe .
This 9s Cated at Goc end Prorconc oelrd (o pered
ficom Collection bollle s-20 Les cne lrasfered

The meth ehe vias are clesed ettb calton flcy

7hese rod vios Cenla$ he Rles arre Kert
10

DROSOPHLLA EGG
E17 aue Small and ellip3cedol 8hared meajenog
O.mma Sn lenjth arnd .2 mm 0) brreth
The o e usaly,laid on the Snfece of he
15 Cutlene mediem.
Dorcs ou szde of theeS s Falter lpn Concare
ventral sede
-Anteret - dorsal lert of the e? eEnfans 0O
Fellemenls.
20 TUo atove feanents cre forned rom exfenSibo
Of the Choro)
Chonien crd Flemenlr ane Seoealed by he
Folite, ceue Stenrurdg he f doeninj Cogenesc
Anter_' e Centen n envelop OPenha or rased
25 Brecteure CaLed os meeophy
SPer ertouhroagh the micro Phye, Por
Teacher's Signature
Experiment Name / No: Camlin Pago No. S
Data

FIRST ,SECenND AD TIRED LARYAL STAGES:

0récR 8Y hes the fertili 2ction e hatches onto
Arst larva.
kßCarcee Feed on yeat CelLS

hoors antour
Ru)AreeR Q hans of hafeg yk onsler lave
Cherges gnto Secend nstor lare.
V)Seeond nsler lona tanj meuth hooks bod
SCfment and eracheo
10 SsLen

PUPA
on the sRth dass the thicd lave slose es
ecleyed feporkm
|G nside Be Panj ne fhe Gody s darilee anto
heod thaox and abdomen.
QßiHeod Contean orfena and compound Ly
(v Abdo men seyments are Cleany zble .
cu Pupa slese leses alaam Por ydas
v Pula mela merthase gnto odece

ADUT MALE An FEMALE DROSO PH/LA

MALE:.
maLe are Smellere thee) Femee.
mto hced, Lhocex abdomen
Heed cenkears lon compourd eges nd a feir
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Experiment Name / No:
Camlin /Paga Wa O6
Data

Head als goined the thoroK aldome) by o

Thorax Conlans ens exlended abdomen .
Abtne Conlon segmenls .
FEMALE!
-Femole are laye then moles
Gody daes2 ble no heed, thorox and abdorgen .
teod Confoans farìned longe blocu Ccmpocrd
eyes ond Puaked oreernae.
10
Thorex Contains geeors f lejs and pahy of

Abdomen Contans 7 Segmens

Herd coog8 gn Folded ondateen extend begened
Lose obdomincl smert
15

20

Ananta Bhoi
25 Cless - t3 32d Yeove
Roll no S137MI200/
Teacher's Signature
 mICROMnERES

BLASTOCGELE
MACRDMERES

(F3- v.S DF Blastula/af Frog)

Experiment Name / No: Camlin Pago No.
Dato

CHARACTERS -
The cell of the. embryo ane Knoon as boto
The maller ceu occo Pyn' onmoes hemess Phere
ond obove he, eruzloriol line ane CclLed
mteromerres
mictOmercs dnded more aridy chich reesalts

as blastocoer orc seymetes Cenby

-7he thin and smál mio meres ene Spread
20 the teoof Of blasto ceel locods onanal fle
FTae beastocse s oCacuted Ly mettilene d blasto -
deno of lange YoKy blastomerey KDYN Q

15

TDENNTIFICATIon
Sue to ebove den kt ony horeckens gt
% adentiied as blastle cof freUs).
20

Anonta Bhoi
25 +3 3rd year Se. Ghser
Roll o! S937mlq00f

Teacher's Signature
 (Fg.
V.s
of C

Gastruta
k
4mESODERIO
of ErupóDE tNDODE
yokyRn yokyRrn trvoTocHoRD
ARCHENTE Ro
frag
Experiment Name / No:
Camlin Pago No.
Date

CHARACTÉRS
9e s frucetauy SPhericol
9e os Caered externotey by everal ayers cf
Small bace Ces Conseelalirg ts eceoderm.
BlasforYe s Complele cwzth a Cicceelat maguh.
Xolk Plef s Seen SLrshleey fraolceclrs aet
fhreab blatoPore.
-Neeral plokeoe beeomes e i denl as nert ol
Polds ben her elevation.
10 morrhoenelc moVement seen cnd Form heeje
layers.

|DDEr°TIFICATION
Due lo bae denl fen Charocle
15

20|

Ananta 3hoi
+3 2rd yecr sc. (6eh se

25

Teacher's Signature
 oLFACT ORY ORGAN

EYE

EXTERNAL GIL

(E. w.m External gell stoje of todole lora

Experiment Name / No: Camlin Pago No. O
Data

CHARACTERS

-The larva Consèses ef body ond tarl

-|The farl s lon? cwith Ushored myotaS
Laleraley Comressed al Fer s presenk
10
7he bdg has a pai of olfeckorg De.

A pairc of eje radidimenls anleriony.

meent develope on he ventral Sde of he
15
body
Cut develored Comtlelely
Dors al and Vetrol Fen Fold ore Presene

20| DDENTTFICATI on
Dee to fhebove chare eng 9
98 sden ltRed os exenol le seoge of
todple Lorvec-)

25 Anonta Bhei
t3 3red yeer s.(6kh sen?

Teacher's Signature
 PROAmIor!
NOTOCHORD
RImITVE STREFK

AREA PE LLUciDA

(Fg. w.m.chiek embeyo at |8 he)

Experiment Name / No:. CamlinPaga No. O
Data

CHARACTERS
-CeLe dzferenlraled eclo derm mesodercm and erdoden
Qre Cleanly 9denlfioalle

-A khs oe he dor RerePhoral arcel oloce

and Central trranslucenl area Pe,lluoida are
dehinit vesible

At the p of the netochod plale develops -

10

Zh rront of nlo chond neral eote derelsres.

18 hons 9n Clu baledembry s celled ag
dzfeernlae s t u toe
15

TDENT]FICATION
Due eo aoUe gdentshns'Chareeeckeg
9t 9s 9denbhed as om of chi embyo
20|
ot L hocrs Of -onCubobn

Ananla Bhoi
25
t2 3rd year Sc. (Gth Sem)
RoL nNos S37m1900

Teacher's Signature
 NURALDDRE
dURAL FuLD

SomiTE

HENSEN NoDE
PRIm/TIUE GGROOVE
AREA OPOCA

(g- w.m of chic embego ot 28 hre)

Experiment Name /No: CamlinPagYNo. /
Data

CHARATERS
o the mesodercm Sn fronl of tHenyeny node , has
ven ise lo arhy of Somzlles
-an ehe regbn of hecl Fold the
5 anteriore part
Of nertal ohOl has hen 'yeo tsk'mt
Fore bren.

The Forejut ond Couredt Vascles ane Scuketaenke

10 deveoIcd
The eKtra embregorie areo has grroon Size.

LDE T/ELCATIOr
lo the obere CAorackeng ths
gs odenkied as c of Chick t a8
hoens f 9ncubalibn.

Ananlo Bhoi
25
t3 3rad year s.6th Ser
Role no! $37ml00

Teacher's Signature
 FORE CRAIN

NURAL CRODVE

AREA VASCULoSA

PRImITIVE STREAK
AREA DEauoA

w.m OF check embryo a 36 hr

(3
Experiment Name / No:. Camlin Pago No. (
Dato

CHARACTERS
The exfrra embronic eo has grocon n S22e
Prooumon zo) hog d'sappeorec.

- 7 s Pimetie slreaK s ComRrkivey reedueed

beccese of freot Lengkh erihg of neura Canal
nd n e l Folds

The brath gs deeernbioles gnto Forre brerin

10
mtd oran end hend baih.
The cardcoc Vesrde has en rse fo heathent

2e 235 Companable coith en Somibe Seaye

15

LDENTIFICAT(Or
Due to ehe abre chareclens ges
denbred os co.m of chick embryo t 36hoca
EXAMINED
20
Examination 20
Dept. of Zoology

Slgn
Anente Bhoi
25 t3 Zred yeer sc.G sen)
Roln! S37m/q00f

Teacher's Signature