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Food Microbiology-An Introduction 5
Food Microbiology-An Introduction 5
Microbial Growth
Foodborne illnesses
Conclusion
Introduction
Food microbiology encompasses the study of microorganisms, which have both beneficial and deleterious effects on the quality, and safety
of food.Food microbiology focuses on the general biology of the microorganisms that are found in foods including: their growth
characteristics, identification, and pathogenesis. Specifically, areas of interest which concern food microbiology are food poisoning, food
spoilage, food preservation, and food legislation. Pathogens in product, or harmful microorganisms.
"The study of food microbiology has come a long way since its beginning in the 19th century. Early pioneers in the field, such as Louis
Pasteur and Robert Koch, laid the groundwork for our understanding of foodborne pathogens and the role of microbes in food spoilage.
MICROBIAL GROWTH
Introduction
In microbiology, a bacterial food source is referred to as the substrate. A substrate may be in the form of meat, vegetables, debris on a
countertop, soil on your hands.Bacteria will reproduce wherever they can find substrates and convert them into usable resources—as long as
the surrounding environmental conditions are suitable. By understanding the conditions under which bacteria reproduce, we can begin to
understand how we can either harness that growth to our advantage (as in the production of cheese and wine) or inhibit the growth to
prevent food spoilage and foodborne illnesses.
Potentially hazardous food (PHF) is the term we use to describe a food substrate in which microbes are able to survive, replicate, and
colonize. The BC Food Premises Regulation (Government of British Columbia, 1999) defines a potentially hazardous food as a “food in any
form or state that is capable of supporting
the growth of disease-causing microorganisms or the production of toxins.” Bacteria can easily grow in some types of food—for example,
raw fish and dairy products—because there are plenty of nutrients and moisture in those foods.
Figure 1 — Bacteria growing on a nutrient agar plate
NUTRIENT AVAILABILITY:
Nutrients availability. Microorganisms need certain nutrients, like carbohydrates, proteins, and fats, to grow. The more nutrients a food
contains, the more likely it is to spoil.This is because the microorganisms use the nutrients to create energy for themselves. So, when a food
has a lot of nutrients, the microorganisms have a lot of energy to grow quickly. This is why canned and processed foods often have a longer
shelf life than fresh foods. They have less nutrients, and are therefore less likely to spoil.And we also have oxygen etc.
FOOD PROCESSING:Food processing is another method that is used to prevent spoilage. Food processing can include methods like
pasteurization, dehydration, and canning. These methods can remove moisture, add preservatives, or otherwise alter the food product to
make it less susceptible to spoilage. Some methods, like canning, can also preserve the food for a long time.
METHODS OF FOOD PROCESSING:
DRYING:Drying, or dehydration, removes the moisture from a food product. This makes it harder for microorganisms to grow and cause
spoilage. Drying can be done in a number of ways, including air drying, sun drying, and freeze drying. Dehydration is often used to preserve
fruit and meat products.
SALTING:Salting is the process of adding salt to a food product to prevent spoilage. The salt creates an environment that's not conducive to
the growth of microorganisms. Salting is commonly used to preserve fish, meat, and vegetables.
ADDITION OF ACID:The addition of acid, like vinegar, lemon juice, or lactic acid, can create an environment that's too acidic for
microorganisms to grow. Acids can also inhibit the growth of some enzymes that can cause food spoilage. This method is often used to
preserve fruits and vegetables.
Other microbes in food:
Pathogenic Bacteria:which are bacteria that can cause disease in humans. Some examples of pathogenic bacteria include Salmonella,
Listeria, and E. coli.
Beneficial Bacteria:Beneficial bacteria are microorganisms that can actually help prevent food spoilage. They can do this by producing acids
that lower the pH of the food or by competing with other microorganisms for nutrients. Lactic acid bacteria, which are used to make yogurt
and cheese, are an example of beneficial bacteria.
Molds:which are a type of fungus that can grow on food. Some molds can cause food spoilage and produce toxins that can be harmful to
humans. However, some molds are actually used in the production of certain foods, like blue cheese. And some molds are used in the
production of medicines and other products.
Yeasts:Yeast is a single-celled fungus that's used in the production of bread, beer, and other fermented foods. Yeast is also used in the
production of some medicines and biofuels. Some types of yeast can cause food spoilage, while others are considered beneficial
microorganisms.
TECHNIQUES
Culture techniques:This is a way of growing bacteria in a controlled environment, such as a petri dish. The bacteria can be grown on different
types of media, such as nutrient agar or blood agar. This technique allows scientists to study the growth rate and other characteristic of the
bacteria.
Molecular technique:which use tools like DNA sequencing and polymerase chain reaction (PCR) to study the genetic makeup of bacteria.
These techniques allow scientists to identify the specific types of bacteria that are present in a food sample, as well as the genes that they
possess.
Mathematical models:Mathematical models are used to predict the growth of bacteria in food. They take into account factors like
temperature, acidity, and nutrient availability to calculate the number of bacteria present at a certain time. These models can be used to
temperature, acidity, and nutrient availability to calculate the number of bacteria present at a certain time. These models can be used to
predict how long a food will stay fresh or when it will spoil. This information is important for food manufacturers and retailers, as it helps
them to plan for storage and distribution of food products.
SOME SPECIFIC EXAMPLES OF HOW THESE TECHNIQUES ARE USED: One example is the use of a growth curve to predict the growth of
Listeria monocytogenes in cheese. This is a pathogenic bacteria that can cause foodborne illness. By using a growth curve, scientists can
predict when the cheese will reach the point where it could potentially cause illness. This information can then be used to ensure that the
cheese is stored and handled in a way that will prevent illness.
Another example:Is the use of PCR to detect the presence of E. coli O157:H7 in ground beef. This is a type of E. coli that can cause severe
illness. By using PCR, scientists can quickly and accurately detect the presence of this bacteria in ground beef.
YEAST FERMENTATION
Yeast fermentation is another type of fermentation that uses yeast instead of lactic acid bacteria. Yeast fermentation is used to make bread,
beer, wine, and many other products. The yeast converts sugars into carbon dioxide and alcohol. The carbon dioxide makes bread rise and
gives beer its fizziness. And the alcohol gives wine and beer their signature taste.
PROBIOTICS
Probiotics are live microorganisms that have health benefits when consumed in adequate amounts. They're found in many fermented foods,
like yogurt, kimchi, and sauerkraut. Probiotics can help improve gut health, reduce inflammation, and even boost the immune system.
Different types of probiotic microorganisms:
The two most common types are Lactobacillus and Bifidobacterium. Lactobacillus is found in yogurt, sauerkraut, and kimchi. It can help
improve digestion, reduce bloating, and even fight off harmful bacteria. Bifidobacterium is found in yogurt and fermented milk products. It
can help with digestion, inflammation, and even allergies.
Probiotics help to balance the microorganisms in the gut, which is called the gut microbiome. They can help reduce the growth of harmful
microorganisms like E. coli and Clostridium difficile. They can also improve the digestive system by producing enzymes that break down
food. And they can even improve the immune system by stimulating the production of antibodies.They can also improve the quality of food
by keeping it fresh for longer and improving its nutritional value.
SPOILAGE
Food spoilage is caused by a number of different factors, including temperature, humidity, and the presence of oxygen. However, the growth
of microorganisms is often the main cause of spoilage. Bacteria and fungi can release enzymes and toxins that cause food to deteriorate and
develop off-flavors. For example, the bacteria that cause spoilage in milk products produce enzymes that break down milk proteins and
cause the milk to curdle.
Types of food spoilage
Rancidity:is a process that causes fats and oils to become oxidized and develop off-flavors. It can be caused by a number of factors,
including exposure to oxygen, light, and heat. Rancidity is often accompanied by the development of free radicals, which can cause other
chemical reactions that further deteriorate the food.
Toxins:Certain microorganisms can produce toxins that can cause illness even after the food has been cooked. The most common example
of this is botulism, which is caused by the toxin produced by Clostridium botulinum. This toxin is not destroyed by cooking, so it's important
to prevent its growth in food. Other examples of toxins that can cause food poisoning include staphylococcal enterotoxin and shiga toxin.
Chemical spoilage: Chemical spoilage is another type of spoilage that doesn't involve the growth of microorganisms. Instead, it's caused by
the natural chemical reactions that occur in food over time. For example, the browning of fruits and vegetables is caused by a chemical
reaction called enzymatic browning. Another example is the development of bitter flavors in potatoes, which is caused by a reaction between
enzymes and sugars.
FOOD ATTRIBUTION
Food attribution is the study of finding out what types of foods are sources of foodborne illness. One of the questions asked during food
attribution research is, “At what point in the food chain does a pathogen originate?”This often happens at the farm level; where failure to
control the pathogen occurs, contamination may take place or be amplified during processing or at home.The most common method of food
attribution is called epidemiological investigation. This involves interviewing people who are sick and gathering information about what they
ate before they got sick. The information is then analyzed to see if there is a pattern that points to a specific food or ingredient as the cause
of the illness.
The critical control points (CCPs) are the steps in a food production process where hazards can be controlled. These are the points where
the greatest risk of foodborne illness exists. Each CCP should have a critical limit, which is the maximum level of a hazard that is acceptable.
For example, a critical limit for pathogenic bacteria in a food product might be zero. If the critical limit is exceeded, corrective action must be
taken to bring the hazard back within the acceptable range.
Control point:There are several different types of control measures that can be used at critical control points. One type is preventive control,
which involves measures that are taken to prevent the hazards from occurring in the first place. Examples of preventive control measures
include proper cleaning and sanitation of equipment, using pasteurized ingredients, and sourcing raw materials from reputable suppliers.
Corrective control is another type of control measure. It involves taking action to correct a problem if the critical limit is exceeded.
Verification:is the process of checking to make sure that the critical control points are being followed and that they are effective. This can be
done by testing for the presence of microorganisms or by reviewing records and monitoring systems.
FOOD FRAUD
Food fraud is the intentional substitution, addition, tampering, or misrepresentation of a food product.Food fraud can have serious health
and economic consequences, and it is a growing concern in the food industry.
Food fraud can take many forms, but the two most common types are adulteration and mislabeling. Adulteration is when a food product is
intentionally diluted or substituted with another substance, such as adding water to juice or using cheaper ingredients in place of more
expensive ones. Mislabeling is when a food product is labeled in a way that is misleading or false, such as labeling a product as organic when
it is not.
METHODS OF FOOD FRAUD
There are many different methods that can be used to commit food fraud. One common method is called "economically motivated
adulteration," or EMA. This is when food products are intentionally adulterated in order to increase profits. Another method is called "pass-
off," which is when a lower-quality food product is sold as a higher-quality product. Food fraud can also be committed through mislabeling,
counterfeiting, or the addition of hazardous or prohibited substances. Another specific method of food fraud is called "substitution." This is
when a food product is intentionally substituted with a similar, but less expensive, product. For example, a fish product may be labeled as a
more expensive type of fish than what it actually is. Another common method is called "fraudulent origin." This is when a food product is
labeled as coming from a certain location or region when it actually comes from somewhere else. This can be done in order to increase the
price of the product or to meet labeling requirements.
Food commonly subject to food fraud: There are a number of foods that are commonly subject to food fraud. One example is olive oil. Olive
oil is often substituted with other types of oil, such as sunflower oil or soybean oil, in order to increase profits. Coffee is another food that is
commonly subject to fraud. In some cases, low-quality coffee is labeled as high-quality coffee. Honey is another food that is often subject to
fraud. It is often diluted with water or sugar syrup in order to increase profits.
CONCLUSION
In summary, food microbiology is the study of the microorganisms that are present in food. These microorganisms can be beneficial, like
those used in fermentation, or they can be harmful, like those that cause foodborne illness. Food microbiology helps to ensure the safety of
the food supply, and it also plays a role in the development of new food products. There are a number of methods for controlling
microorganisms in food, including sanitation, food processing, and food packaging. Certification standards help to ensure that the food
industry is meeting the highest standards for food safety.