[S] VoA

1.50E-05 0.21
2.00E-05 0.25
3.00E-05 0.28
4.00E-05 0.33
8.00E-05 0.44
1.60E-04 0.4

1/[S] 1/VoA
6.67E+04 4.76
14.00
5.00E+04 4.00
12.00 3.33E+04 3.57
f(x) = 0.00011109128375077 x + 4.80121005908081
R² = 0.991971255656207 2.50E+04 3.03
10.00
1.25E+04 2.27
8.00
6.25E+03 2.50
6.00

4.00 f(x) = 4.04919355706431E-05 x + 2.04850851992526

R² = 0.965097266811302
2.00

0.00
b
00 04 04 04 04 04 04 04 04
0E+ 0E+ 0E+ 0E+ 0E+ 0E+ 0E+ 0E+ 0E+ m
0 0 0 0 0 0 0 0 0
0. 1. 2. 3. 4. 5. 6. 7. 8.
Vmáx
KM

Y= mx + b

2.05E+00
 VoB
0.08
0.1
0.12
0.13
0.16
0.18

1/VoB
12.5
10.0
8.3
7.7
6.3
5.6

A B
2.05 4.8
4.00E-05 1.00E-04
0.49 0.21
1.95E-05 2.08E-05

Y= mx + b
 [S] Vo sin Inh VoA VoB 1/[S]
5.00E-04 1.25E-06 5.80E-07 3.80E-07 2.00E+03
1.00E-03 2.00E-06 1.04E-06 6.30E-07 1.00E+03
2.50E-03 3.13E-06 2.00E-06 1.00E-06 4.00E+02
5.00E-03 3.85E-06 2.78E-06 1.25E-06 2.00E+02
1.00E-02 4.55E-06 3.57E-06 1.43E-06 1.00E+02

Sin Inh A B Inh A/Sin Inh

b 195750 202647 592580
m 302.77 760.07 10104.9
3.00E+06
Vmáx 5.11E-06 4.93E-06 1.688E-06 9.7E-01
KM 0.0015 3.75E-03 1.705E-02 2.42E+00
2.50E+06

2.00E+06

1.50E+06

1.00E+06

5.00E+05

0.00E+00
0.00E
1/ Vo sin Inh 1/VoA 1/VoB
8.00E+05 1.72E+06 2.63E+06
5.00E+05 9.62E+05 1.59E+06
3.19E+05 5.00E+05 1.00E+06
2.60E+05 3.60E+05 8.00E+05
2.20E+05 2.80E+05 6.99E+05

Sin Inh vs Inh A VS Inh B

3.00E+06

2.50E+06 f(x) = 1014.94152056237 x + 592579.521577988

R² = 0.999787595895644

2.00E+06

f(x) = 760.071796158345 x + 202647.004364165

1.50E+06 R² = 0.999947197973117

1.00E+06

f(x) = 302.772945387481 x + 195749.879895635

R² = 0.999728217816058
5.00E+05

1/ Vo sin Inh
0.00E+00 Linear (1/ Vo sin Inh)
0.00E+00 5.00E+02 1.00E+03 1.50E+03 Linear 2.00E+03
(1/ Vo sin Inh) 2.50E+03
1/VoA
2.50E+03
 [S] Vo/E-6
2.50E-05 38
4.00E-05 53.4
6.00E-05 68.6
8.00E-05 80
1.60E-04 106.8
2.00E-04 114

1/Vo
b 0.0062 0.0300
m 5.00E-07
0.0250 f(x) = 5.01336520524702E-07 x + 0.00623427359032246
Vmáx 161.29 R² = 0.999983489885181
KM 8.06E-05 0.0200

0.0150

Ec Michaelis-Menten 0.0100

0.0050

0.0000
0.00E+00 1.00E+04 2.00E+04 3.00E+04 4.00E+04 5.0
1/Vo Linear (1/Vo) Linear (1/Vo)
Vo
3.82E+01
5.35E+01
6.88E+01
8.03E+01
1.07E+02
1.15E+02
 1/S 1/Vo
4.00E+04 0.0263
2.50E+04 0.0187
1.67E+04 0.0146
1.25E+04 0.0125
6.25E+03 0.0094
5.00E+03 0.0088

0623427359032246

3.00E+04 4.00E+04 5.00E+04

(1/Vo) Linear (1/Vo)
 [S] Vo sin Inh Vo con Inh 1/S
0.299 0.071 0.018 3.344
0.5 0.1 0.03 2.000
0.82 0.143 0.042 1.220
1.22 0.25 0.07 0.820
1.75 0.286 0.105 0.571
2.85 0.353 0.159 0.351
5 0.4 0.2 0.200
5.88 0.5 0.25 0.170

Chart Title
60.000
f(x) = 16.3143279169339 x + 1.28168595233922
50.000 R² = 0.995851796014997
40.000

30.000

20.000

10.000 f(x) = 3.89851503103982 x + 1.51040093132574

R² = 0.985740641332949
0.000
0.000 0.500 1.000 1.500 2.000 2.500 3.000 3.500 4.000

1/Vo Sin Inh Linear (1/Vo Sin Inh)

1/Vo Con Inh Linear (1/Vo Con Inh)
 [I]=200 micromolar
INHIBICIÓN COMPETITIVA

1/Vo Sin Inh 1/Vo Con Inh

14.085 55.556
10.000 33.333
6.993 23.810
4.000 14.286 Km [I]
3.497 9.524 4.946
2.833 6.289 KI
2.500 5.000 50.76142
2.000 4.000

Sin Inh Con Inh

b 1.51 1.28
m 3.89 16.31
Vmáx 0.662 0.781
KM 2.576 12.742 (Aumenta)

3.000 3.500 4.000

1/Vo Sin Inh)

1/Vo Con Inh)
(VERIFICAR)