Br Rekhaforchi ) ONA hybucu2 a on and doxon0 ny Shy:

VA yeatni
RNA Nuclele eud
aucl bybadbzatior) 36//ra
ONN Hhe gcndic
genee malesad Whus a
maekuað of an orgoniGn be d owoos
badouuma Aunlo uwa plont o elebhant ov kumanbong
an

has due do
(M
)s
Y1) Hhe mole
lineak
alaj uagrt
Even n
deguenee o base pos
hose oqarisms whcte Hhe moleuta wigut
He ineas Aegu ene o base pau
halees weii)
dijosent b4se

3 lhe ma
mun aum to Jind out
ol modn axonomg
helationskip betwan ongorisms on bosis o qaulalle
Jato and knowledge oNA JoNt u a good lool. One
technigue employd u oNA hybi dizalon
an oYganism is heated,He dwo slranlc Aepahate
due to "weake ring ol hudogen bonds belwcen he
base þau, and temp,aite d wkich it dokes place as
caued
mdung
CooledHor U
point Hhe hot boludion s gaaduay
bases in two seþa,ot e tands agaun
His þaoess
þho cesS w caled
called Rnne
He o hot Boludi ons Cont Anne aling oling .On He
he contA0
awni ng Ainqle strand 2N
quuckly cooled tht base pau i joint and the
strands hoU u to Johm andor
cails
single
in which Gome
pai kuing ol base on Hhe
do ccu Such
basis o Com plimentaily
anclom cls
Jham
to_etLo n a solut 'on wluch
u alow ed to Coo oduaUy, H,e basc in Hh
 Single sMands
Mands o 9NA om dillesent þecies woutd foh
He basis
l Compi menlauty and þouial
doulle Sands NA woud be Jormed Tuis iu
Rnown s ONA hybkidization, The } ojsNA ybriduz
indicates He d helatiorssib beewuH Hhe two s
Thi's Jechnigue Sona iX Com patea aud iu nowaday
by molecul a ayenomstc to asseA S he dA« o
helationslip bchuen ovqanjsms. Lls gheat advantg
H,a aNA omhorm veçetaire þat Can Bhew helokionshab
and eve, Jdenaie&tion. In His method Hehe iu no ned
it
laluet 9NA no deeles
J ao bossihle do
by corpokaton
uncorpohaton o kadioaotire
OY ) issue cultuue ols
culs dn
þhos þho 0G.
la Costlg (1964) lecnigue involvet Hhe
The badon - 9NA 3trand oj
inculotion dogethea o) ingle soand JongJab eled stngle
0ne
peue (known at oive SNA) t,
strardsNA broken op nto Ahott deng hs by blgsica
theotment o anothes þeeie c known a traca NR)
The
Jhagment Jend do adhae do the comþlimentahs
potions lo Aong NR strands by ubsipucnty
out don SNA sthands om te mirtue.
and dd e rmuu'ng Ae extent do whih Hese have
become Aadiodive, a measue can be obtained q)
 242
iatdon(19(u) Jovnd Ahad only hal| nucleotide krguente
| i n Hhe NA ol vica wlleco a,
,r Jimlo, (homolog ou)
, wit Ahose o bisum whide ls Ha ahe homo Jogous beun
Phas eolus qnd P'som
hee has becn Conside%, olle advan cement in heeento
d mo wbossi ble to beak ( cleave) NA at hguy Ipeujr
þornts using
uslng estiion endonucleas es, eot ol wwr
Can cdeave oNA at a higuty Apeuic þointe osig
CCn ceave
hestcti on endonudeases, ach o which
SNA a a wgly Abeu'ic y boints heeogri ion kite
we chasacfeistic
(nucdeoti de Aeguene) Hins þho dueing hgy
Aetic'on agmend f DNA. and
These Can be bepahat ed by elecrophosesiS
s aclioadve Single strarnded CNA
hgbidise wat
methoe! has bean ied ith encousoging
Paobes Ti's bevchal ote genso
Secate and
Aeults dn Ruplex, obgenha il not ound
Ablex diston tndo Jus
In
Cohet
(<þNN )i lavouile ol Suendists
chloroblosl NA 9enomes i, nvde
oHheh dwo Cellula
Cemþa'son to
Tuis o because cpavn
ond mitochondiua genom analysed and il unot
(an be Casily ilodated and omalycod al 9ene
þhocessu Auch
oltered by evodatiòn aky
evokeivn
clopicdion and Conceled
 alho has an added
org0nizabon, Se Ohd pimab,
Conseaved tn B& assica have bton
eguene stodiet o chONA maland þrogtoe o)
in esdalbrlong
abopolg þlods. As clovoplost genomt possess He
the hgbid þlont wl
mate natly
mala
mudeog maleias o| both pas ents but ony the c+ c
matehna pakert These te chnigue along
Computa aid u nowdoys used by molecalag
Aoronoymsts Jo ass ess He degteey hdationilips
ad vantage ie Hhat sNRS
betuun organisns 11
Jom veg eai ve bast ean Ahow Adationit anc

Jderd calon Jh His metod Hhee n0 nud

04gan ihe Jouwh and

By deleh rninali'on ofl nomal bases:

Hhjs mehod te phohoktions ol fou nohnd
basa- aderi'ne, Thyamuhg ylosine and guanne
dna NA i dete, mined
and compa. cd writth othd pecteg
(vanples o ONA hybidiz alion tn davonony
1) SNA o Tutcqe wh el, baley and hy)
compased wtth hat ol Ayen
hoen ere ond Ahowcd e
malked dleenca Jheat i mote
The oNN
Bimulas do Aeyeye sto hal ba ley
 sheua ol Aduiplex (chenopodiatcae)
wae Comþ a ed on hebosis oDNA
nudeode:
moleculey pobes y Smau

Seyment natecenigecl. eompl emetery 5egben Ce.

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