1) Screen and select three phytoplankton species possible for bioremediation.

2) Enhance the cultivation conditions for the chosen phytoplankton species

with regard to:

a) Light Intensity

b) Temperature

c) pH test

3) Semi-mass produce the selected phytoplankton species.

4) Determine the physico-chemical parameters of the aquaculture wastewater:

a) Chemical Oxygen Demand

b) Nitrate

c) Nitrite

d) Ammonium

e) Total Phosphorous

5) Examine the impact of varying phytoplankton concentrations on the

effectiveness of phytoplankton-based aquaculture wastewater treatment in

removing pollutants in terms of:

a) Algal Growth

b) Water Quality

c) Chlorophyll Content
 DATA TO BE GATHERED

Table 1. The Three Phytoplankton Species Isolated in the Study Area Selected for
Bioremediation

Phylum Species

Photomicrograph under the compound microscope of the three selected

Phytoplankton Species

Fig. 1.
Phytoplankton 1

Fig. 2.
Phytoplankton 2

Fig. 3.
Phytoplankton 3
 Optimization of Growth Conditions on the Three Selected Phytoplankton
Species

Table 2. Mean Specific Growth Rate of Phytoplankton (per day) at different light
intensities at constant pH and temperature

Phytoplankton Light Intensities

Table 3. Mean Specific Growth Rates of Phytoplankton (per day) grown at different
temperature at constant pH and light intensity

Phytoplankton Temperature

Table 4. Mean Specific Growth Rate of Phytoplankton (per day) subjected to different
pH at constant light intensity and temperature

Phytoplankton pH
 AQUACULTURE WASTEWATER

Tale 5. Physico-chemical parameters of the aquaculture wastewater

Chemical Nitrate Nitrite Ammonium Total pH Dissolved

Oxygen Phosphorus Oxygen
Demand

Impact of Varying Phytoplankton Concentrations on the Effectiveness of

Phytoplankton-based Aquaculture Wastewater Treatment in Removing
Pollutants

Table 6. Specific growth rate and Doubling Time of Phytoplankton Species with
Different Inoculum Concentrations in Aquaculture Wastewater

Phytoplankton Initial Inocula Specific Growth Doubling Time

Species Concentration Rate

Table 7. Analysis of the Aquaculture Wastewater Quality after the Bioremediation

Procedure

Chemical Nitrate Nitrite Ammonium Total pH Dissolved

Oxygen Phosphorus Oxygen
Demand
Figure 4. Chlorophyll Content of Phytoplankton Species with Different Inoculation
Concentrations in Aquaculture Wastewater