QUIZ 1

ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.

1. Primary goal of fixative

A. Hardens tissue
B. Prevent further trauma
C. Preserve in a life-like manner
D. Easy cutting

2. Ideal fixative to tissue ratio

A. 30:1
B. 20:1
C. 5:1
D. 1:1

3. Ideal time of fresh tissue

A. 15 mins
B. 30 mins
C. 45 mins
D. 60 mins
4. pH of buffered: 7.0
5. Which of the following is not a characteristic of a good fixative?
A. It is safe to handle
B. Rapid penetrate tissues
C. Improve staining
D. It slowly kill cells thereby producing minimum distortion of cell constituents
6. All of the following are cytoplasmic fixatives, except
A. Reygaud’s fluid
B. Orth;s fluid
C. Newcomer’s fluid
D. Kelly’s fluid
7. Tap water is used in washing out except:
A. Excess osmic acid
B. Excess mercuric fixatives - kase Alcoholic Iodine is used instead
C. Excess formalin
D. Excess chromates
8.
9. Protein tertiary structure
10. Should be noted except:
a. Location
b. Color
c. Size
d. Lymph Node
11.
12.
13. Mandated by the Department of Health as part of licensing and accreditation
requirement/s:
 QUIZ 1
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
A. Standard Operating Procedures
B. Material Safety Data Sheet
C. Either
D. Neither
14. Hypochlorite is considered as/an:
A. Irritant
B. Corrosive
C. Either
D. Neither
15. Eyewash station must be situated ___ feet from hazardous work areas
A. 10
B. 50
C. 75
D. 100
16. Lab microwave differs from domestic microwave.. The former
A. Produces uneven heating
B. Adequate ventilation
C. Produces hotspot
D. Doesn’t require precise temperature
17. during staining the optimum temp nc microwave process ng non metallic
answer: basta may 58 - 55-60
18. Potassium dichromate
ans : Mordant
23. Ensures that pathological areas are identified
a. Serial sections at 2 mm thick
b. Cutting 1 cm thick sections
c. Opening the organ
d. Get a representative of normal tissue
24. Solution if sections stick to microtome
a. Increase humidity
b. Breathe on the block
c. Either
d. neither
26. Floatation bath
- 55-60
27. Solidified paraffin wax
- 2 hours

28 melting point of paraffin wax

- 56-58

29 sliding microtome
- Celloidin…
30. Sharpening of knife, EXCEPT
 QUIZ 1
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
HEADINGS

31. It is soluble in alcohol, ether, benzene, chloroform, acetone, and xylene. (Tri-ethyl glycol)
32. Prolonged exposure will not affect the tissue; will not cause tissue disruption. (Dioxane)
33. May be used for demixing, clearing and dehydrating paraffin sections and before and
after staining (Tetrahydrofuran)
34. Causes the tissue to ribbon poorly (Dioxane)
35. Most Urgent Biopsy

Clearing agents
A. Xylene
B. Benzene
C. Toulene
D. Chloroform
E. Cedarwood oil
AB. Carbon tetrachloride
AC. Alanine
AD. Clove oil
AE. tetrahydrofuran

B36. Clearing time 15-60 mns

AB 37. Similar properties with chloroform but cheaper
C 38. Recommended clearing time (1-2 hrs)
D 39. Used for clearing embryos, insects, and very delicate specimens.
A 40. Colorless clearing agent that clears ½ to 1 hour.

41. Suitable for specimens with large hollow cavities – Celloidin

42. Melting Point 56-57 deg C – Paraplast
43. Used when tissue is subjected for histochemical and enzyme studies – Carbowax
44. Best embedding medium used for routine processing – Paraffin
45. Not soluble in water, but soluble in 95% Ethyl Alcohol – Ester wax

True or False
46. Hard tissues can be washed with … 4% aqueous shit. TRUE
47. Chloroform-fixed tissues are washed with tap water prior to staining.
48. Cellosolve is a slow-acting dehydrating agent but is not harmful to the tissue. FALSE
49. Acetic acid crystallizes at 17C
50. Picric acid as a fixative causes swelling in tissues

51. microchatter edges around tissue of small biopsy

A. excessive dehydration
B. Xylene with excess water
C. Open processor dry up tissue
 QUIZ 1
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.

52. effect of immediately transfer of hardened tissues with high concentration of alcohol
A. maceration
B. Hardening
C. Shrinkage
53. Clearing agent turn milky as soon as the tissue is placed
1. Overfixation
2. Excessive Dehydration
3. Over washing

54. Cause/s of autolysis

1. Under Fixation
2. Incomplete Dehydration
3. Insufficient fixative volume
MORSE
55. Dilution dehydration uses
A.50-60
B. 60-70
C. 90-95
56. Post fixation treatment of this fixative is
thoroughly rinses in water uses
A.Bouins
B. Zenkers
C.Hellys
57. Properties of tissue that affects processing
1. Solvent effects
2. Tissue density
3. Fixation

58. Microanatomical fixatives include:

1. Brasil’s
2. Heidenhain’s
3. Bouin’s

59. Overdehydaration
1. Shrinkage
2. Brittleness
3. —-
60. Good clearing agent
1. Miscible with alcohol
2. Miscible with paraffin
3. Miscible with aniline
 QUIZ 2
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
1. A group that gives color and is a basis for the general classification of dyes is termed:
A. Auxochrome
B. Chromophore
C. Either
D. Neither
2. Which of the ff is performed after "bluing"?
A. Nuclear staining
B. Cytoplasmic staining
C. Dehydration
D. Differentiation
3. If the tissue stained H&E and the nucleus is dark blue/purple: Basophilic, hematoxylin
4. What is the dimension of the cover slip: 24×50
5. What is the effect of having excess xylene in the slide.
A. Rapid setting of mounting medium
B. Bubble formation
C. Either
D. Neither
6. Using of mounting media
A. Protect the stained section from scratches
B. Prevent the distortion of image during microscopic examination
C. Either
D. Neither
7. What is the action of xylene after staining with H & E?
8. Alternative for ammonia water as bluing agent
A. Lithium carbonate
B. Distilled water
C. Either
D. Neither
9. Refractive index of coverslip: 1.518
10. Acid component of decoloring agent: sulfuric acid?
11. this is the step performed prior to staining
A. Drying in paraffin on at 60 degrees celsius
B. Immerse the slide in second charge of xylene
C. Either
D. Neither
12.Which of the following dyed contain glacial acetic acid
A. EFAS
B. Lithium
C. Og6
D. hematoxylin
13. All are performed after draining the water from the slide after floating except:
A. Fixing at 60C hot-air sterilizer
B. Use of adhesives like 1% gelatin
C. Fixing at 37C incubator
D. Fixing at 60C hot plate
14. Component of Mayer’s Egg Albumin:
E. Formalin
F. Methanol
 QUIZ 2
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
G. Glycerin
H. Thymol
15. Paraffin sections float when added to.. Because of
A. Air bubbles
B. Incomplete infiltration
C. Either
D. Neither
16. Opaque and ..
A. Insufficient clearing
B. Contaminated mountant
C. Either
D. Neither
17. presence of fat and oil
lipophilia
lipidemia
Sundanophilia
18. Tissue that will be stained by strong acid or alkali that contains glycogen
a. Paraffinization
b. thio…
19. BLUING AGENT AT WHAT TEMP -
20. COUNTERSTAIN -
21. Romanowsky (Neutral)
22. Giemsa (Neutral)
23. Methylene blue- basic
24. Picric acid- acid
25. Irishman’s stain - Basic
26. Glacial acetic acid -

27 contains absolute alcohol - Harris

28. Example of Iron Hematoxylin - Weigerts
29. Mixture of two solutions
30. For immunohistochemistry

MODIFIED TRUE OR FALSE

A. 1 TRUE, 2 FALSE
B. 1 FALSE, 2 TRUE
C. BOTH TRUE
D. BOTH FALSE

33. Natural dyes include orcein, aniline, and saffron. (F: Aniline is synthetic.)
Natural dyes are derived from plants and animals previously used in dyeing wool and
cotton. (T)
B

34. Artificial ripening agents for hematoxylin include sodium permanganate, mercuric oxide,
and potassium iodate. (F: Sodium iodate, not permanganate.)
Ripened hematoxylin uses chloride salts as mordants. (T)
 QUIZ 2
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
B

36. Warm tap water is used as bluing agent. Bluing agent in ammonia??? Is more rapid
chuchu than warm tap water, help hehe.

D 37. Phenol - 0.5 to 5% (4%)

Aniline - 200 ml 1/2 to 1L of hot dh2o

C 38. Elastic: Verhoeff, Weigerts

Elastic: Black and Dark Blue

C 39. Lissamine fast red would stain muscle orange-red. Congo red would stain amyloid red.
B 40. Faded sections may be re-stained by immersing the slide in xylene by 24 hours then
stained immediately by a new stain. The most common technique in histopathology is
Hematoxylin and eosin staining technique.

MATCHING

Column A Column B Column C

E 41. Reccommended for 46. A. Glycerin Jelly (B) A. Resinous

small tissue sections but not 47. B. Canada Balsam (A) B. Aqueous
for whole mounts 48. C. Farrant's Medium (B) C. Not Listed
B 42. Refractive index of 49. D. Brun's Fluid (B)
1.524 50. E. DPX (A)
A 43. Standard mounting
medium when dehydrating
and clearing with xylene
cannot be made
C 44. Contains gum arabic
E 45. Contains spirits of
camphor

Column A Column B Column C

56. A. Inadequate A. Ensure that sections

51. Nuclear staining is too
dark
52. Nuclear staining is not
crisp. D and A
53. Pale compressed
cytoplasmic staining - A.
Water causes pale staining,
thus, if there is inadequate
dehydration water is still
present in the sample hence
dehydration are not too thin
57. B. Sections are too thick B. Dry slides at proper
() temperature
58. C. Metallic sheen is C. Check staining
seen on top of slide (D) procedure
59. D. Heat is prolonged D. Filter stains daily
60. Sections are too thin E. Change alcohol and
xylene solutions
 QUIZ 2
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.

the answer
54. Metallic sheen on top of
the slide - C
55. Slides are milky or hazy
in the last xylene - A
 QUIZ 3
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.

1. Ideal thickness of bone for decalcification is 1-3 cm.

If bone is thicker, decalcification is prolonged

2. Ideal fixative is carnoy's fixative.

Immediate diagnosis is needed, fixation can be skipped.

5. Most common chelating agent-EDTA

6. Poorly fixed specimens-MACERATED
7. Freeze drying (-30C to -40C)
8. Freeze substitution
9. Cartilage does not require decalcification. FALSE
Toe nails require decalcification. FALSE
11. Chelating agents are most common decalcifying agent
An example of a chelating us verscene
12. Ion exchange resin is a process that removes positively charged calcium ions when
attracted to a negatively charged.
This method is not recommended for mineral containing acids like hydrochloric acid
13. Sectioning of tissues using cryostat starts at 20um. TRUE
After sections are cut, a glass slide is placed on top of the tissue to pick it up. TRUE
14. The fixative used after the freezing medium is melted is 10% neutral buffered formalin.
TRUE
Tissues can be stored in cryostat for an extended period of time if tissue is wrapped and
sealed air-tight. TRUE
15. Increase decalcification, increase destruction TRUE
At 37C impaired ..
16. Decalcify & softens- PERENYI’S
17. Component is HCL- VON EBNER’S
18. Can be used for decalcification and fixing- CHROMIC ACID
19. TOO SLOW FOR ROUTINE TESTING - PERENYI’S
20. MOST RAPID - PHLOROGLUCIN NITRIC ACID
21. Perenyi - 2-7 days
22. Phloroglucin -12-24 hours
23. Von Ebners - not listed
24. Flemings - not listed
25. Formic Acid-Sodium Citrate - 3-14 days
CELLS FOUND IN CERVICOVAGINAL SMEARS
A. Pregnancy Cells
B. Navicular Cells

25. Formic Citric acid buffer -

26. XX chromosome - barr bodies
28. Latter menstrual - Navicular
29. 15-30 in diameter- Superficial
30 Shed-ovarian hormone - ENDOMETRIAL

33. Cytoplasm is finely vacuolated. - Intermediate cells

34. Appearance of cell is due to glycogen accumulation. - Pregnancy cells
 QUIZ 3
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
35. Estrogen-Progesterone effect - Navicular Cells

CRYOSTAT TEMPERATURE

A. -15C to -25C
B. -35C to -45C
C. -10 to -15C
D. -25C to -35C
E. -45 to -55C
AB. 0C to -15C
C 36. Uterine curettage
D 37. Breast (fatty)
A 38. Skin without fat
A 39. Lymph node
D 40. Lung
A 41. Prostate
AB 42. Testes

-10 to -15 C
A. Testes
B. Uterine curettage

-15 to -25 C
A. Others (pinakamadami)

-25 to -35 C
A. Breast (fatty)
B. Fat
C. Lung
D. Skin with fat

A. Quadrant Vaginal Scrape

B. Vulvar Scrape
C. Vaginal Scrape
D. Lateral Wall
E. Endocervix

B 46. For evaluation of herpetic lesions

C 47. For patients with hysterectomy
A 48. For the localization of vaginal adenosis
D 49. Used for hormonal evaluation
B 50. Used for evaluation of herpetic carcinoma
51. Prostatic M(?)
52. Sputum
 QUIZ 3
ANG TANGA MO KUNG IKAKALAT MO PA ‘TO, GAGO.
53. Gastric brushing
54.
55. Urine
1-15 Code of Medical Technology
16.
17.
18.
19. Evaluate employees in the laboratory every:
a. Annually
b. Semi - annually
c. As needed
d. Employees feedback immediately
20.medical laboratory technician can process this except:
a. Clinical microscopy
b. Histopathology
c. Parasitology
d. Clinical Chemistry
25. Quality check of signed out reports. EXCEPT. - Receiving samples (?)
26. MT/MLT should be reviewed every after
a. 6 months
b. 12 months
c. 24 months
d. 36 months
27. Refusal for newborn screening- religious belief
28. In quality control, +2 standard deviation eme eme -
a. 50
b. 75
c. 95
d. 98
29. Direct, indirect cost - STANDARD COST
30. Personnel information reqs on the chain of custody is -
a.NAME AND SIGNATURE
b. address
c. Birthday
d. position in the company
31. The following are given the MT eme EXCEPT: MT American base on US pero nasa
SULU
32. General term for formal recognition eme - ACCREDITATION
35.Create newborn kineme - RA

NASA RAYMUNDO “TO CHECK NIYO “HUMAN RESOURCE MANAGEMENT”

A. Histotechnician C. Associate pathologist
B. Pathologist D. Grosser Associate pathologist
B. 36. Establish policies and procedures on recruitment and appointment
D.39. Describe the specimen in detai, making drawing, taking photographs, if necessary.
A. 40.Ensure tissue processor with tissue sections are not overload
B 41. Conducts strategic plan at least annually.
42. Performs periodic reviews of quality management systems.
C 43. Monitor staff by filling out quality control sheets
D 44. Properly identify and match the specimen with the request
A 45. Readily?? filter especially hematoxylin
 A. Blood Bank Law
B. Clinical Lab Law
C. HIV Law
B. Clin Lab 46. RA 4688
C. HIV 47. RA 8504
A. BB 48. RA 7719
A. BB 49. AO 9 s. 1995
C. HIV 50. PNAC Resolution 1

A. Convention
B. Self- direct learning
C. In program training
D. Residency
E. CPE Provider
51. Post graduate specialization
52.Gathering of licensed professionals that includes round table discussion
53. Completion of post-doctoral training program in a specified field
54. Includes workshop and scientific meetings
55. Apprentice training at the graduating level
56. Learning that uses accredited learning module
57. Also known as externship training
58. HIV proficiency and Drug testing proficiency

64. DOH AO 2007-0011

65. DOH AO 118B S. OF 1992