How does Film Containing Stem Cell Secretome can Healing Burn Wound on Male Wtihe Rats?

Methods Results

1. Made some gel and film secretome 1. The Effect of Secretome Films on the Healing Results of Macroscopic Second Degrees Burns in
corresponding author/researcher make 3 formulas with secretome as active Male White Rats
compound. the first formula contains 5% secretome, secound formula
contains 8% secretome and third formula contains 10% secretome.

2. Preparation of test animals and induction of burns in experimental animals

Male rats aged 12 -14 weeks and weighing 200 – 250g,, the hair on the back was shaved and then anesthetized
with diethyl ether,and than burn were made with a metal chop which was heated to 95 ± 20C in water and
touched to the rat's skin for 10 seconds.
Figure 1. Results Healing of Macroscopic Second Degrees Burns in Male White Rats
3. Burn Healing Activity Test
The test animals given secretome primary dressing films according ti their respective concentrations. primary 2. The Effect of Secretome Films on the Healing Results of Microscopic Second Degrees Burns in Male
film dressing was given for 7 days, and observations were made everydays. After 7 days, the rats were White Rats
euthanized using diethyl ether and then the skin was cut on the back and immersed in a 10% Buffered Normal
Formalin (BNF) solution.

4. Histological Analysis of Skin Test Animals

Observed changes and repairs that occur in cells or tissues. Changes and repairs are assessed by meansof
scoring according to the severity and wound healing process which can be seen from the severity of
inflammation, the formation of collagen and fibroblasts and re-epithelialization. Scoring is done with numbers
1 to 4 where 1 is a small amount, 2 is a moderate amount, 3 is a large amount, and 4 is not found.
Figure 2. Results Healing of Microscopic Second Degrees Burns in Male White Rats

Conclusion References

• Mathen, C., Mrunal, G.S., Raghubansh, G., et al. 2021. Evaluation of potential application of Wharton’s jelly-derived
human mesenchymal stromal cells and its conditioned media for dermal regeneration using rat wound healing model. Cells
Tiss. Org, 210(1), pp. 31–44.
Primary dressing films containing mesenchymal stem cell secretome have gone through various
• Laila, L., Febriyenti, F., Salhimi, S.M. et al. 2011. Wound healing effect of Haruan (Channa striatus) spray. International
tests both macroscopically and microscopically and obtained good results in inhibiting damage Wound Journal. 8: 484-491.

caused by heat on day 5 thereby accelerating the healing of second degree burns on the skin of • Ratih, L., Noor, US., Sumiyati, Y., et al. 2022. The effect of mesenchymal stem cell-conditioned medium gel on burn
wound healing in rat. Veterinary World, 15(4): 841-84
male white rats. • Aryan, A., Mohaad, B., Shahin, B., et al. 2018. Human bone marrow mesenchymal stem cell conditioned medium promotes
wound healing in deep second-degree burns in male rats. Cells Tissue, pp. 317–329.
• Abbas OL, Özatik O, Gönen ZB, et al. 2018. Prevention of Burn Wound Progression by Mesenchymal Stem Cell
Transplantation: Deeper Insights into Underlying Mechanisms. Ann Plast Surg. 2;81(6):715-724.