NÜVE SANAY MALZEMELER

MALAT VE T CARET A. .

ES 120 / ES 252

COOLED INCUBATORS

USER’S MANUAL

Z14 K25 233 Rev.No:01 Rev.Date: 02 / 2010

Manufacturer:

NÜVE
SANAY MALZEMELER
MALAT VE T CARET A. .

Esenboga Yolu, 22. Km.

06287 ANKARA
TURKEY
Tel: 00 90 312 399 28 30(3 lines)
Fax:00 90 312 399 21 97
e-mail: sales@nuve.com.tr

WARRANTY CERTIFICATE

1. Nüve warrants that the equipment delivered is free from defects in material and
workmanship. This warranty is provided for a period of two years. The warranty
period begins from the delivery date.

2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided
with the equipment.

3. Nüve does not accept any liability in the case where the goods are not used in
accordance with their proper intent.

4. The warranty may not be claimed for damages incurred during the shipment, for
damages resulting from improper handling or use, the defects in maintenance,
negligence, bad functioning of auxiliary equipment, in the case of force majeure or
accident and incorrect power supply.

5. In the event of failure, Nüve shall be under no liability for any injury, or any loss or
damage as the result of the failure other than the guarantee conditions.

BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE READ

CAREFULLY.

INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF NÜVE.

IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT HIS PERMISSION.

THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF

THE INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS MANUAL.

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TABLE OF CONTENTS Page

1 INTRODUCTION 3

1. Use and Function 3

2 TECHNICAL SPECIFICATIONS 4

2.1. Technical Specifications Table 4

2.2. General Presentation 5
2.3. Optional Accessories 5

3 INSTALLATION PROCEDURE 6

3.1. Lifting and Transport 6

3.2. Unpacking 6
3.3. Environmental Conditions 6
3.4. Mains Supply 7
3.5. Positioning 7
3.6. Prior to Incubation 7

4 OPERATING PRINCIPLES 8

4.1. Switching On 8
4.2. Display and Control Panel 8
4.2.1 Explanations and Functions for Display and Control Panel 8
4.2.2 Operating the Control Unit 11
4.2.2.1 Programming Summary 11
4.2.2.2 Program Repetition 12
4.3. Completion of the Operation 12

5 PERIODIC MAINTENANCE & CLEANING 13

5.1. Periodic Maintenance 13

5.2. Cleaning 13

6 TROUBLESHOOTING 13

6.1. General Troubleshooting 13

6.2. Error Codes 14

7 ELECTRICAL CIRCUIT DIAGRAM 15

2
1. INTRODUCTION

1.1. USE AND FUNCTION

The ES 120 / 252 Cooled Incubators are used to obtain temperatures from -10 ° C to
60 ° C in the chamber. They keep the set temperature stable during the operation.
The incubators are used for cold storage, incubation, heating and drying applications.

Stable and homogeneous temperature in the useful volume is provided by means of

the forced air circulation through the stainless steel heater and the cooling
evaporator. The insulation, which is proportional to the dimensions of the incubator,
not only provides a homogenous temperature distribution but also makes the
operation of incubator economical.

The samples can be observed through the heat treated glass door. The chamber is
illuminated by the lamp which is on when the outer door is opened.

The incubator ensures reliable working conditions by the programmable

microprocessor controlled main PCB which has a very high control accuracy.
Adjustable safety thermostat offers an additional safety.

Do not operate the cooled incubator for purposes other than main purpose.

The cooled incubator is only to be used by authorised people after the user’s
manual has been read carefully. Only technical personnel can handle the
product in case of a failure.

The ES series incubators are manufactured according to the following

standards

EN 61010-1, EN 61000-6-3.

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2. TECHNICAL SPECIFICATIONS

2.1. Technical Specifications Table

TECHNICAL SPECIFICATIONS ES 120 ES 252

Temperature Range - 10 ° C / + 60 ° C
Temperature Sensor Pt 100
Thermostat Adjustment Sensitivity 0,1 ° C
Temperature reading sensitivity 0,1 ° C
Temperature Homogeneity ± 1°C
Timer 100 Hours and Hold position
No of program memory and step 9/9
Safety system Gas expansion safety thermostat
Defrost Automatic
Refrigerant R134a
Power supply 230 V 50 / 60 Hz
Power consumption 800 W 1250 W
Useful Volume 120 Liters 252 Liters
Internal material Stainless Steel
External material Stainless Steel, Epoxy polyester painted
Internal Dims ( W x D x H ) mm 475 x 545 x 475 475 x 545 x 970
Overall Dims ( W x D x H ) mm 650 x 740 x 1010 650 x 740 x 1640

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2.2. General Presentation

1 2 3 4 5

6 7

5
1. Door 5. RS 232 Connection Terminal
2. Observation window 6. Power cable connector
3. Display and Control Panel 7. Safety thermostat
4. On/Off Switch

2.3. Optional Accessories

a. Shelves and shelf carriers

b. Data control software and RS 232 interface.

3. INSTALLATION PROCEDURE

3.1. Lifting and Transport

Due to the heavy weight of the unit, all handling must be carried out by using proper
handling equipment. The unit must be supported from underneath and never be
turned over.

3.2. Unpacking

Remove the packing cardboard box and the second nylon packing around the cooled
incubator. The below written are provided with the equipment, please check them;

• User ‘s Manual
• Power Cable
• 2 shelves for ES 120 and 3 shelves for ES 252
• 2 shelve carriers per 1 shelf

3.3. Environmental Conditions

Please pay special attention to the followings,

Indoor use only

Ambient temperature: 5 ° C to 40 ° C
Humidity level % 80 up to 22° C
Maximum height 2000 m.

The maximum performance is obtained between 15 °C and 25 ° C.

IMPORTANT : The instrument is designed to operate in 20 ° C ( ± 5 ° C ) laboratory

conditions. The efficiency of the unit decreases if the ambient temperature exceeds
the limits.

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3.4. Mains Supply

The cooled incubator requires 230V, 50 / 60 Hz.

Please make sure that the supplied mains matches the required power ratings.

Always plug the cooled incubator to correctly grounded sockets.

A supply fitted with a circuit breaker should be used for protection against
short-circuit in case of an isolation fault.

3.5. Positioning

Lift the cooled incubator carefully with proper handling equipment, and carry it to its
place.

Balance the incubator on the four pedestals. If necessary, adjust the height of the
pedestals. Insert shelf carriers and the shelves.
Check the followings,

• The proposed site is suitable for users,

• The operator can follow up the unit even he deals with something else.
• The cooled incubator does not occupy the utilization space of others or damage
them.
• There is enough space (at least 20cm.) from the cooling unit ventilation.
• Leave at least 20cm. on the back side of the unit.

3.6. Prior to Incubation

Plug the instrument in to a grounded socket.

Check the followings,

• Make sure that the safety thermostat is adjusted to the temperatures which are
higher than the set temperature.
• Liquids are not heated in sealed containers,
• The boiling point of the samples are higher than the set temperature.
• The freezing points of the samples are lower than the set the temperature.
• The liquids which may expand during heating do not overflow from their
containers,
• The set temperature does not destroy the structure of the samples,
• The vapor and gases which are generated during the operation are not harmful to
human health or flammable or explosive,
• Set the safety thermostat. (the safety thermostat set value should always be set a
value that is higher than the working temperature.)
• Plug the power cable into a grounded socket.
• The instruments, which will be dried up and heated, should not be combustible
and explosive, especially please kindly check that.

IF MENTIONED WARNINGS ARE NOT CONSIDERED, NUVE WILL NOT BE

RESPONSIBLE FROM THEIR RESULTS.

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4. OPERATING PRINCIPLES

4.1. Switching On

Push the On/Off switch.

See that the microprocessor control system activates.

Set the values and start the operation.

4.2. Display and Control Panel

4 15 1 3 2 13 11 14

16 9 10 5 6 8 7 17 18 12 19

4.2.1 Explanations and Functions for Display and Control Panel

01- Temperature Display

This display shows :

• Temperature inside the chamber during the operation and stand-by.

• The temperature and alarm set value during programming.
• The temperature and alarm set value during the control of set values.
• The EoFF message incase of electrical interruption.
• Error codes incase of failure.

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02. Heating Signal LED

It shows that the heating process has started. This LED flashes during heating.

03-Cooling Signal LED

It shows that the cooling process has started. This LED flashes during cooling.

04- Alarm Signal LED

Alarm signal LED flashes continuously if any alarm condition occurs.

05-Time Display

This display shows;

• The time set value during programming.

• The elapsed time during the operation.
• “Hold” during programming and the operation when the timer off is selected.
• “Cont” during the control of set values.
• The time set value during the control of the set values.

06-Timer Off LED

The “Timer-Off” should be selected to operate the cooled incubator at hold position.
The “Hold” is shown at the Time Display and this LED remains on until the program is
stopped manually by using the STOP key.

07- Timer-1 LED

If the Timer-1 option is selected, the timer counts up the time after the temperature
has reached the lower value of the set alarm limit. When the program finishes, the
incubator turns to stand-by position and the Timer-1 LED flashes until the STOP key
is pushed or the incubator is switched off.

08-Timer-2 LED

If the Timer - 2 is selected, this LED turns on and the timer starts to count up the time
when the program is started. At the end of the program, if the stop button is not
pushed the same program starts again and is repeated for the repetition time. For
repeated programs Timer-2 should be chosen. At the end of the repetition, the
Timer-2 LED flashes and the instrument turns to the stand-by position.

09- Program No Display

This display shows,

• The number of the program which is operating.

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• The number of the program during the programming.
• The number of the program during the control of the set values.

10-Step No Display

This display shows;

• The number of the step which is operating.

• The number of the step during the programming.
• The number of the step to be checked during the control of the set values.

11-Program No Key

This key is used :

a) To set the number of the program during programming.

b) To call up the program to be operated or checked.

The program number is increased by pushing PROG NO key.

12-Step no Key

This key is used :

a) To set the number of the steps during programming.

b) To call up the step to be checked.

The program number is increased by pushing PROG NO key.

13-Set Mode Key and LED

The programming process is started by pushing this key. The LED turns on when the
key is pushed.

This button is pressed again to exit from the programming mode.

14- Value Increase and Decrease Keys

This key is used to increase the set values in the related display. Every push results
in one unit increase. To rapidly increase the values, push the key continuously.

15- Alarm Mute Key

This key is pushed to mute the audible alarm.

16- Illumination Control Key

This key is used to light on or off the lamp inside the chamber.

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17- Repetition Key

This key is used to adjust the repetition number of the program will be operated.

18- START Key and LED

The program can be started to run by pushing this key.

The LED of this key turns on during the operation and turns off after the completion
of the program.

19-STOP Key and LED

This key is used to:

a) Validate the parameters and save them in the memory during programming.
b) Stop the running program manually.
c) Cancel the alarm.

4.2.2 Operating The control Unit

When the incubator is switched on “-----“ is seen on the temperature display, “P” is
seen on the program display, “t” is seen on the step no display and “set9” is seen on
the time display for 5 seconds.

4.2.2.1 Programming Summary

Press PROG. NO key in order to choose the number of the program to be adjusted.
Press STEP NO key in order to adjust the step number of the program to be
adjusted.

Press SET key and see its LED lightens. “dLY” will be seen on the
temperature display, the program number of the related program will be
seen on the program no display, “-“ will be seen on the step no display
1 SET
and “00:00” will be seen on the time display. Adjust the delay time of the
operation by using the value increase and decrease keys.
Press STOP key and adjust the temperature value that flashes on the
2 STOP
temperature display by using the value increase and decrease keys.
Press STOP key, “ Alr “ and alarm difference temperature value will flash
3 STOP alternately on the temperature display. Adjust the alarm difference
temperature value by using the value increase and decrease keys.
Press STOP key and adjust the timer mode between the choices Timer-
4 STOP off, Timer-1 andTimer-2 by using the value increase and decrease keys.
Press STOP key and adjust the time value by using the value increase
5 STOP and decrease keys unless the chosen timer mode is Timer-off.
6 STOP Press STOP key.
7 SET Press SET key to exit from the programming mode.

By using the table below, adjustments for 9 programs and for each step of each
program can be performed.

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NOTE:

• According to programming summary table, at the 4th step if "TIMER OFF" is

selected , Hold appeares on the TIME display, if “TIMER1” or “TIMER-2” is
selected 00.00 appeares on the TIME display.

• If “TIMER OFF“ is selected, the program operates only the first step temperature
value until the program is stopped manually.

• For the programs with Timer-1 or Timer-2 modes the steps, times of which are
adjusted to 00:00 are skipped during the operation.

• Any program can be operated but all programs start to run from the 1st step. Time
should be adjusted for the 1st step, otherwise Err6 alarm is seen on the display.

• Push the VALUE INCREASE key continuously for 5 seconds to see the set
values while the cooled incubator is running. First “Cont“, and then “Set“ are
shown on the Time Display. Release the key when “Set” appears. The set values
will be shown on the related displays and the LEDs of the related keys turn on
sequentially. This cycle is repeated twice.

• To control the parameters for nine step of cooled incubator push the STEP NO
key during controlling.

• To control the parameters for other programs of cooled incubator push the STEP
NO key during controlling.

4.2.2.2 Program repetition

In order to set the repetition number of the program that will be started, press the
repetition key (17). The repetition number already adjusted will be seen on the
temperature display, program number will be seen on the program display, “-“ will be
seen on the step display and “rPtn” will be seen on the time display. Adjust the
repetition number by using the value increase and decrease keys and press STOP to
validate. The repetition number should be adjusted and saved for each program. As
soon as you press the START key the program starts to run and it repeats as many
as the repetition number. You can observe the elapsed repetition number by pressing
the value decrease key during the operation.

NOTE: Program repetition is possible for only Timer-2 mode.

4.3. Completion Of The Operation

• See that the program is over, lead the instrument to the STOP position
• Take the samples out. Be careful while handling the samples after the operation
as they can be hot.
• Wipe the chamber surface if needed when the chamber is cold enough.
• You may leave the incubator at the stand-by position or switch it off

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If the unit is in START position in case of the open door, it will keep operating
and the heaters will be over-heated. Besides, the heaters and other
components may be defected. Please be careful.

5. PERIODIC MAINTENANCE AND CLEANING

5.1. Periodic Maintenance

The cooled incubator does not require any periodical maintenance which should be
carried out by the operator.

Please contact to Nuve agent for your servicing and preventative maintenance
requirements.

5.2. Cleaning

• After every operation wipe down the chamber surface to remove any undesirable
effects of the operation, such as spillage.
• You may use a soft brush to clean the chamber.
• Check regularly and clean the condenser, according to the dirtiness of the
environment.
• For the external body, you may use a piece of cloth. Mild detergent use is
recommended to remove difficult dust and dirt.
• Please aware of the undesirable effects of the chemicals and be careful while
applying them.
• Protect the chamber against rust coming from outside.

6. TROUBLESHOOTING

6.1. General Troubleshooting

If the cooled incubator fails to operate, check the followings;

• The on / off switch is on.

• The mains supply is present.
• Fuses are sound.
• The plug is plugged in properly.
• The plug is not defective.
• The installation of the plug is not defective.

If the cooled incubator does not heat, check the followings;

• The program has started.

• The safety thermostat is set at a temperature higher than the set temperature.

If the temperature exceeds the set value, check the followings;

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• The internal circulation fan operates.

If the cooled incubator does not cool, check the followings;

• The internal circulation fan operates,

• The ambient temperature does not exceed 20 ± 5°C limits.

6.2. Error Codes

In case of failure during an operation, below written codes are shown on temperature
display and the alarm sounds.

Err 1 : Thermocouple ends are broken.

Err 3 : The measured temperature value exceeds the limits of measurement range.

Err 5 : There is a failure about the cooling system.

Err 6 : Timer-1 (or TIMER-2) is selected but no value is set for the time for any step.

EoFF: This code appears in case of a power failure if the power failure causes the
temperature inside the chamber to go beyond the alarm limits.

OPEN: The door stayed open for more than 1 minute during the operation.

HıGH or LoU: The measured temperature exceeds the set alarm limits while
operating on the set temperature

If the temperature is higher than the set temperature by a value greater than the
alarm limit it shows HıGH, if the temperature is lower than the set temperature by a
value greater than the alarm limit it shows LOU.

In case of any error, the program is stopped automatically.

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7. ELECTRICAL CIRCUIT DIAGRAM

1.

15