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Растениевъдни науки, 2018, 55(1) Bulgarian Journal of Crop Science, 2018, 55(1)

Effect of different ratio and intercropping systems on forage


yield and some components of Hungarian vetch (Vicia
pannonica Crantz.) and grass combination under arid climate
conditions
Ferhat Demirhan1, Adnan Orak2*, Hazım Serkan Tenikecier2
1
Ministry of Food, Agriculture and Livestock, Edirne Directorate of Provincial Food Agriculture and
Livestock, Edirne, Turkey
2
Tekirdağ Namık Kemal University, Faculty of Agriculture, Field Crops Department, Tekirdağ, Turkey
*
E-mail: aorak@nku.edu.tr

Abstract
Demirhan, F., Orak, A. & Tenikecier, H. S. (2018). Effect of different ratio and intercropping systems on
forage yield and some components of Hungarian vetch (Vicia pannonica Crantz.) and grass combination
under arid climate conditions. Rastenievadni nauki, 55(1), 44-52
Intercropping productivity under conventional farming system was carried out in the experimental field of The
Trakya Agricultural Research Institute (TTAE) in Edirne province in 2012-2015 growing seasons with 3 replica-
tions on randomized complete block design. The experiment consisted of mainly 7 entries for yield components
- i.e., three pure stands Hungarian vetch (HV), oat (O) and annual rye grass (ARG) and four different mixtures
(66% HV+34% O, 34% HV+66% O, 66% HV+34% ARG, 34% HV+66% ARG) as follow. Seven traits includ-
ing plant height (PH), stem diameter (SD), leaf/stem ratio (LSR), legume ratio in botanic composition (LRBC),
legume fresh forage yield (LFFY), total fresh forage yield (TFFY), total dry forage yield (TDFY), plant height
(PH), stem diameter (SD) and leaf/stem ratio (LSR) were measured on 10 random (vying plant) points per plot.
Legume ratio in botanic composition (LRBC), legume fresh forage yield (LFFY), total fresh forage yield (TFFY),
total dry forage yield (TDFY) were recorded at each plot. In mixtures of Hungarian vetch with annual rye grass,
average forage yield (21.86 t ha-1) was lower by 32% than (28.87 t ha-1) that in mixtures of Hungarian vetch with
oat average yield. Consequently all of the investigated parameters in the present study, 60% or 40% HV mixture
with oat combination, can be recommended for high yield forage production.
Keywords: Hungarian vetch; rye grass; oat; intercropping productivity

INTRODUCTION 2002). Other benefits include water quality control


through minimal use of inorganic nitrogen fertilis-
Mixed culture (or intercropping) of legumes and ers that pollute the environment (Crew and Peoples,
cereals is an old practice in agriculture that dates 2004).
back to ancient civilization. The current trend in For example, studies have shown that mixtures
global agriculture is to search for highly productive, of cereals and legumes produce higher grain yields
sustainable and environmentally friendly cropping than either crop grown alone (Dapaah et al., 2003).
systems (Crew and Peoples, 2004). When two crops In such crop mixtures, the yield increases were not
are planted together, interspecific competition or only due to improved nitrogen nutrition of the ce-
facilitation between plants may occur (Zhang and real component, but also to other unknown causes
Li, 2003). The main objective of intercropping has (Connolly et al., 2001). In addition legume is a natu-
been to maximise use of resources such as space, ral mini-nitrogen manufacturing factory in the field
light and nutrients (Li et al., 2003), as well as to and the farmers by growing these crops can play a
improve crop quality and quantity (Mpairwe et al., vital role in increasing indigenous nitrogen produc-

44
tion. Legume help in solubilizing insoluble P in soil, port for climbing vetches, improve light intercep-
improving the soil physical environment, increasing tion, and thus facilitate mechanical harvesting (Na-
soil microbial activity, and restoring organic matter, deem et al., 2010). For instance, HV can be grown
and also has smothering effect on weed. The car- with forage grasses or cereals under Mediterranean
ryover of N derived from legume grown, either in conditions, during which the fallow+cereal system
crop sequence or in intercropping system for suc- is performed.
ceeding crops, is also important. The other one of the most important forage crops
Vetches (Vicia sp.) are the most widely distribut- is annual ryegrass (ARG) (Lolium multiflorum
ed annual leguminous crops throughout the Medi- Lam.), which is a cool-season grass that is suitable
terranean basin, western Asia and in countries of the for quality herbage production on account of its rich
former Soviet Union (Martiniello and Ciola, 1995; protein, minerals, and water-soluble carbohydrate
Dhima et al., 2007; Yolcu et al., 2010). It can be used content (Kusvuran and Tansi, 2005, 2011). It is gen-
for pasture or as grain legume, showing high palat- erally a highly nutritious grass that may be presented
ability at all growth stages. Because of its high feed as forage for beef cattle through grazing, dried out
value for animals it is often used as grain for live- and fed as hay, or ensiled and fed as silage (Durst
stock feed (mainly lambs) and also for production of et al., 2013) and desirably eaten by livestock, espe-
silage and hay or as green manure (Açıkgöz, 1988). cially in milk production (Kusvuran, 2011). Cereal
Mixtures of legumes with cereals are expected species, seeding ratios, and competition between
to have advantages over pure stands in terms of mixture components may affect yield and quality of
forage yield and quality. In vetch-cereal intercrop- forage produced by mixtures (Papastylianou, 1990).
pings, cereals provide structural support for vetch Caballero and Goicoechea (1986) and Thomson et
growth, improving light absorption and allow- al. (1990), reported that the most suitable cereal for
ing mechanical harvest (Lithourgidis et al., 2006). a mixture with common vetch is oat (Avena sativa
Many previous studies have reported that intercrop- L.), whereas Thomson et al. (1990) and Roberts et
ping with legumes can achieve an enhance biomass al. (1989) reported that barley (Hordeum vulgare
and yield over corresponding monoculture (Zhang L.) and wheat (Triticum aestivum L.) are the most
et al., 2011; Arshad and Ranamukhaarachchi, 2012; suitable cereals for mixtures. Other studies show,
Huňady and Hochman, 2014; Zafaranieh, 2015). however, that spring barley is a better component
Different small grain cereals and vetches have for self-finishing cultivar of vetch compared to oats
been successfully used in cereal-legume intercrop- (Księżak, 1997).
ping systems (Karagić et al., 2011). Although there Competition of different species can also have
is a large diversity of Vicia species in the Mediter- a significant impact on growth rate of the used in
ranean Basin, including Turkey and Thrace region mixtures. The aim of the present study were to
vetches, particularly common vetch (Vicia sativa evaluate Hungarian vetch, oat and annual rye grass
L.) and Hungarian vetch (HV) (Vicia pannonica monocultures as well as mixtures of Hungarian
Crantz.) are the most common annual forage crops vetch with oat and annual rye grass in two seed-
cultivated (Uzun et al., 2004). HV is a winter-har- ing ratios (66:34 and 34:66) for forage yield and its
dy (-16°C) legume species, which is widely used in components.
Thrace region with cool winter growing conditions.
It has satisfactory forage yields with tiny plentiful
and palatable leaves, and good quality hay with high MATERIAL AND METHODS
crude protein (CP) (Tuna and Orak, 2007; Unal et
al., 2011). Moreover, that is generally recommended The evaluation of Hungarian vetch, cv “Budak”
in dry regions (Uzun et al., 2004). HV has a semi (HV) cereal oat, cv “Kahraman” (O) and annual rye
erect growth habitus with a leaning tendency in pure grass, cv “Grass” (ARG) intercropping productiv-
stands, especially during rainy years. Intensive ear- ity under conventional farming system was carried
ly spring rains lead to the decay of plant parts close out in the experimental field of The Trakya Agri-
to the ground to, as a consequence of high humidity. cultural Research Institute (TTAE) in Edirne prov-
This results in a decrease in the rate of forage yield ince (41°65´ N, 59°68´ E). The experiments were
and quality (Iptas, 2002). Cereals can provide sup- conducted for three consecutive growing seasons

45
(2012-2013, 2013-2014 and 2014-2015). The soil of ic composition (LRBC), legume fresh forage yield
the study site has been classified as mainly clay. The (LFFY), total fresh forage yield (TFFY), total dry
experimental area has an arid climate with hardy forage yield (TDFY), plant height (PH), stem di-
winters and hot dry summers. In the experimental ameter (SD) and leaf/stem ratio (LSR) were mea-
location, a total precipitation amounted to 615.1 mm, sured on 10 random (vying plant) points per plot.
486.0 mm and 674.0 mm during the growing season Legume ratio in botanic composition (LRBC), le-
(October-July) of 2012, 2013 and 2015, respective- gume fresh forage yield (LFFY), total fresh forage
ly, while a 30-year average amounted to 547.7 mm. yield (TFFY), total dry forage yield (TDFY) were
The precipitation distribution significantly differed recorded at each plot.
over year, hence in February and March of 2015, the The harvest time was determined by taking the
precipitation amounted to 615.1 mm and 674.1 mm, physiological periods of the HV into consideration.
respectively, that was higher than the long-term av- Hence, plots were harvested at the 50% flowering
erage (547.7 mm). Long term temperature condition and initial pod-set stage for the HV and at the be-
(11.6°C) was lower than in all years of investigation ginning of flowering for the ryegrasses (at the end
(14.6, 12.6 and 12.7°C). With regard to climatic re- of May) (Kusvuran and Tansi, 2005) at the same
quirements of these crops, it appeared that condi- time for the forage yield. Following the harvest, the
tions were normal for the growth and development HV ratios in the mixtures were determined in the
in experimental years 2012-2015. fresh material.
Cultivars and their mixtures were grown in a Analysis of variance of experimental results was
randomized complete block design with three rep- calculated according to randomized complete block
lications. The experiment consisted of mainly 7 en- experimental design with three replications using
tries for yield components - i.e., three pure stands MSTAT-C statistical software package (MSTAT-C,
Hungarian vetch (HV), oat (O) and annual rye grass 1988). Significant differences among the mean val-
(ARG) and four different mixtures (66% HV+34% ues were compared by LSD test (P<0,05) (Duz-
O, 34% HV+66% O, 66% HV+34% ARG, 34% gunes et al., 1987). Cluster analyses was performed
HV+66% ARG) as follow. with SPSS 22.0 (IBM, 2013) statistical software.
Seeds were planted at a depth of 3 cm in October
of the first, second and third year, at rate of 100 kg
ha–1 for HV, 180 kg ha–1 for O and 30 kg ha–1 for ARG RESULTS AND DISCUSSION
in each plot. Each plot consisted of six rows spaced
0.25 m apart and 5 m long, occupying an area of 7.5 Yielding of legume-cereal mixtures largely de-
m2. All plots in each replication were separated by pends on the proper selection of species. Yield and
a 0.5 m buffer zone, and replications were separated yield characteristics averaged over-three year data of
by a 2.5 m buffer zone. The crop seeding prior to the Hungarian vetch and its mixture combinations with
experimental area were applied appropriate cultural oat and annual rye grass are presented in Table 1.
practices (deep summer field plow, disk harrowing, Mixture combinations showed significant effects
cultivation, hand weeding, etc). No agrochemicals on total fresh, total dry and legume fresh forage
were applied either on the previous crop (wheat) or yield and all yield components. Plant height, stem
on the mixture combinations. diameter, leaf/stem ratio and legume ratio in botan-
The HV and its mixtures with O and ARG in- ic compositon were remarkable affected. All Hun-
dividual were sown in the same rows by hand. The garian vetch mixtures with oat and Italian rye grass
size of each plot was 7.5 m2 (1.5×5 m). During the combination had higher yields than pure Hungar-
first, second and third year, the experiments were ian vetch during three production years. The yields
carried out without supplementary irrigation. Four of combinations varied based on species and ratio
2.5 m rows at the center of each plot were harvested of oat and annual rye grass used in mixtures. High
with 4.0 m2 area. Control by hand weeding was car- mixture rates of oat and Italian rye grass tended to
ried out twice when the weed density was high, in produce higher forage yield in all individual (not
the pre-flowering and postflowering stages. Seven shown) years as well as for the combined years (Ta-
traits including plant height (PH), stem diameter ble 1). Similar results were also reported by Cacan
(SD), leaf/stem ratio (LSR), legume ratio in botan- and Yilmaz (2015).

46
Plant height vetch – annual rye grass sowing because oats was
Yield and yield components of Hungarian vetch more competitive than annual rye grass.
(HV) with different rate combination of oat (O) and According to the average value while the high-
annual rye grass (ARG) are given in Table 1. There est vetch rate in dry matter was determined from
were significant differences between the mixture the mixture containing 66% Hungarian vetch and
rates. The heighest planth height was detemined 34% annual rye grass (42.88%). The mixture con-
from pure oat (86.22 cm). The heighest planth height taining 34% Hungarian vetch+66% oat had the
of HV (55.00 cm) was obtained from 34% HV+66% lowest vetch rate in dry matter. There were dif-
ARG which was followed by 66% HV+34% ARG ferences between oat and annual rye grass with
(53.78 cm). The mixtures of Hungarian vetch with vetch. Our results showed the percentage of vetch
oat and annual rye grass showed similiar planth in vetch+annual rye grass mixture (42.88% and
height when they grew in monoculture. Plant height 27.75%) were found to be higher than vetch+oat
of oat (86.22 cm) was found to be lower than Tuna (32.38% and 16.63%) combination in both combi-
and Orak (2007) findings (128.8 cm). nation rates (66:34 and 34:66). According to pre-
vious studies, while Yolcu et al. (2010) found the
Stem diameter average vetch rate as 34% in different HV-cereal
With regard to the different mixture ratios, the mixtures, Orak and Nizam (2012) found rates of
highest stem diameter was obtained from pure oat 42% in a 50% HV+50% cereal mixture. Our re-
(3.26 mm) which was followed by pure ARG (2.55 sults were found to be similar to their findings.
mm). Howerver pure and mixture combination of Separately, according to two years average results
Hungarian vetch stem diameter measurements were of Kusvuran et al. (2014) found a vetch rate of
found to be lower than of pure oat and annual rye Hungarian vetch ratio in botanic composition 76.1
grass. and 57,5% in HV+ARG mixture yield were found
to be higher than sowing rate of Hungarian vetch
Leaf/stem ratio ratio, 66% and 34% respectively. Vetch ratio de-
Leaf stem ratio represents the leaf weight di- creased in mixture sowing with annual rye grass,
vided by stem weight. In pure stand, mean maxi- because of more competitive habit of annual rye
mum leaf stem ratio was recorded in ARG (2.86) grass (ARG) in mixture and may originate from
followed by HV (1.75) and O (0.74) (Table 1). Leaf/ differences of region and climate conditions.
stem ratio in crops decreased with the advance-
ment of crop growth stage and increase in stem Forage yield
thickness. In mixtures and pure stand of HV, maxi- Our findings confirmed that the increased seed-
mum leaf stem ratio (1.79, 1.78 and 1.78) was noted ing rate of Hungarian vetch increased fresh forage
in 34% HV+66% ARG, 34% HV+66% O and 66% yield in Hungarian vetch-oat intercropping (Table
HV+34% ARG combinations respectively. Kilcher 1). Similar results have been reported in triticale-
and Troelsen (1973) measured the leaf/stem ratio vetch intercropping (Albayrak et al., 2004). Our
of two oat varieties at flowering and dough stages findings also suggested that when scaling the oat,
to be 1.4 and 0.6 respectively. Response of increas- Hungarian vetch were physically supported by oat
ing competitiveness of oat based on an increase in plants, and this resulted in better establishment and
its mixture rate was observed in leaf/stem ratio of development (Karagić et al., 2011; Atis et al., 2012).
Hungarian vetch. Thus the highest leaf/stem ra-
tio (1.79) in 34:66 (HV+O) ratio was realized. The Legume fresh forage yield
lowest ratio was found to be 1.75 in pure sowing of Legume fresh forage yield of mixtures were af-
Hungarian vetch. fected by seeding ratio of Hungarian vetch in all
mixture combination. Proportions of Hungarian
Legume ratio vetch decreased as the percentage of cereal seed in-
The proportion of Hungarian vetch in dry for- creased in the mixtures (Table 1). The highest le-
age was lower to the proportion of Hungarian vetch gume fresh forage yield (6.97 t ha-1) was recorded
in the seed mixture at all sowing ratios. The pro- from 66% HV+34% O mixture and 66% HV+34%
portion of vetch in vetch-oats hay was higher than ARG combination with 6.80 t ha-1 except pure Hun-

47
garian vetch sowing. Green herbage and hay yield triticale and monoculture common vetch. The other
values derived from the sole sowing of HV were re- researchers have found that all Hungarian vetch-ce-
ported as 6.2-17.0 t ha–1 and 1.7-4.0 t ha–1, respec- real intercropping systems had higher yields than
tively, in the previous studies (Karadag and Buyuk- pure Hungarian vetch during both and the yields of
burc, 2004; Balabanli and Turk, 2006; Unal et al., intercropping systems varied based on species and
2011; Albayrak et al., 2011; Orak and Nizam, 2012). ratio of cereals used in mixtures (Acar et al., 2017).
Our findings were found to be similar to those from Some previous findings showed that differences
previous studies. in yields of mixtures were mainly because of dif-
ferences in tillering characteristics, climate and soil
Total fresh forage yield requirements in mixtures. In mixtures of Hungari-
The greatest total fresh forage yield (29.41 t ha-1) an vetch with annual rye grass, average forage yield
was obtained from 34% HV+66% O combination. (21.86 t ha-1) was lower by 32% than (28.87 t ha-1)
In addition, forage yield was positive affected using that in mixtures of Hungarian vetch with oat aver-
of oat in mixture. In addition, forage yield was not age yield. In addition, both mixtures of Hungarian
affected by the increase of Hungarian vetch ratio in vetch with ARG (21.86 t ha-1) produced about 73%
mixtures. Competition between component species more forage yield than the monoculture Hungar-
in a mixture may affect the yield and quality of for- ian vetch (12.61 t ha-1), in addition about 2% more
age produced. However, forage yields of monocul- than the monoculture ARG (21.41 t ha-1). Both mix-
tures and annual rye grass mixtures with Hungari- tures of Hungarian vetch with oat combinations
an vetch were lower than yields of oat mixtures with (66:34 and 34:66 seeding ratios), however, produced
Hungarian vetch. Similarly Roberts et al. (1989) total fresh forage yield about 124% and 133% re-
found that DM decreased with increasing common spectively, more forage yield than the monoculture
vetch ratios in mixtures with wheat. In contrast, Hungarian vetch 12.61 t ha-1, in addition about 14
Caballero et al. (1995) reported that mixtures of and 19%, respectively, more than (24.83 t ha-1 ) the
common vetch with oat produced 34% more forage monoculture oat (Table 1). Similarly, Glacomini et
yield than common vetch alone, but 57% less than al. (2003) reported that yield of mixtures was simi-
monoculture oat. Lithourgidis et al. (2006) reported lar to that of oat and greater than that of monocul-
that forage yield was higher in cereals monocul- ture common vetch. Balabanli et al. (2010) reported
tures as well as in mixtures of common vetch with that green forage yield of HV+O mixture was 17,75
oat compared with mixtures of common vetch with t ha-1 , our findings was found to be higher than their

Table 1. Forage yield and yield components of Hungarian vetch and combine with oat and annual rye grass,
data are the means of the 3 years
Total
HV ratio HV fresh Total dry
fresh
Plant height Stem diameter Leaf/Stem in botanic forage forage
Mixture rates forage
(cm) (mm) ratio composition yield yield
yield
(%) (t ha-1) (t ha-1)
(t ha-1)
HV 53.44b - 1.84 - 1.75b 12.61c 2.81d
O - 86.22 - 3.26 - 0.74 - - 24.82ab 7.70a
ARG - 63.44 - 2.55 - 2.86 - - 21.41b 5.55ab
66%HV+34%O 53.67ab - 1.83 - 1.76ab - 32.38b 6.97a 28.33a 7.37a
34%HV+66%O 51.67b - 1.79 - 1.78a - 16.63d 3.91c 29.41a 7.79a
66%HV+34%ARG 53.78ab - 1.80 - 1.78a - 42.88a 6.80a 20.56b 5.23c
34%HV+66%ARG 55.00a - 1.83 - 1.79a - 27.75c 4.33b 23.15b 5.93b
Average 59.60 - 1.87 - 1.78 - 30.04 4.53 22.90 6.05
LSD (%5) 1.48 ns 0.03 - 0.43 0.39 0.50 0.06

48
results (66:34 and 34:66 mixture; 28,33 and 29,41 (r=-0,569**) correlation was observed between to-
t ha-1, respectively. The other researchers findings tal fresh forage yield and legume ratio in botanic
(Palágyi et al., 2012) have been found higher (40,3 t composition (Table 2).
ha-1) than our results. Acording to Table 2, in Hungarian vetch mix-
ture with oat and annual rye grass, HV plant height
Total dry forage yield (r=0.693**) showed the significant direct effect on
According to Table 1, the heighest total dry forage total dry forage yield and moreover, forage yield
yield was found to be 7.79 t ha-1 for 34% HV+66% O also was positively associated with stem diameter
in combined analysis of the years. However, forage (r=0.570**).
yields of all mixtures with oat were found to be sta- In mixture, stem diameter had significant posi-
tistically similiar to yield of oat (7.70 t ha-1) in mono­ tive direct effect on plant height (r=0.921**) and,
culture. In addition, pure ARG dry forage yield moreover stem diameter was negative and insignifi-
(5.55 t ha-1) was lower than the monoculture oat and cant correlated with leaf/stem ratio (r=-0.024). This
both mixture Hungarian vetch with oat combination relationship isn’t a clear case of component com-
(66:34 and 34:66 seeding ratios), respectively. The pensation in determining stem dimeter of HV, and
lowest total dry forage yield (2.81 t ha-1) was ob- indicated a trend for leaf/stem ratio to decrease as
tained from pure Hungarian vetch. The results ob- stem diameter increased.
tained by Palágyi et al. (2012) for pure HV, pure O
and HV+O mixture (8.1, 14.5 and 13,6 t ha-1 respec- Cluster analysis
tively) are higher than our results (2.81, 7.70 and 7.79 Cluster analysis is a tool for classifying objects
t ha-1, respectively). Some researchers have reported into groups. Hierarchical cluster analysis was done
5.17 t ha-1 of Hungarian vetch and oat mixture. Their using unweighted pair group method (UPGMA)
results was found to be lower than our findings (7.70 (Sneath and Sokal, 1973). The dendrogram gener-
t ha-1 and 7.79 t ha-1). Different application, region ated by Unweighted Pair Group Method using Av-
and climatic condition may effect the results. erage (UPGMA) based on SM (Simple Matching)
correlation showed clustering of pure Hungarian
Correlation vetch and addition oat and annual rye grass mix-
Achieving the high yield through major yield ture with Hungrian vetch combination traits into
attributes requires the knowledge of the magni- three groups at a 45% similarity level (Figure 1).
tude of correlation among various yield compo- The first group consist of 3 mixture combinations
nents. Correlations between important characters 34% HV+66% ARG, 66% HV+34% ARG and 66%
for mixture and monocultures showed that total HV+34% O which are all high mixture fresh and
fresh forage yield had a highly significant correla- dry forage yield, the highest plant height and leaf/
tion (p≤0.01 and p≤0.05) with total dry forage yield stem ratio in mixture combinations and the second
(r=0.994**), plant height (r=0.717**) and stem di- cluster consist of 34% HV+66% O, which is the
ameter (r=0.594**), while negative and significant highest mixture fresh and dry forage yield, the low-

Table 2. Correlation coefficients between different traits and total forage yield of mixtures and monoculture
of Hungarian vetch
Traits TFFY TDFY LFFY PH SD LSR BOTCOM
TFFY 1
TDFY 0,994** 1
LFFY -0,147 -0,192
PH 0.717** 0.693** 0,440** 1
SD 0.594** 0.570** 0,517** 0.921** 1
LSR -0.094 -0,080 0,234 -0.015 -0.024 1
BOTCOM -0,569** -0,616** 0,803** -0,064 0,043 -0,204 1

49
Figure 1. Dendogram of pure Hungarian vetch, oat, annual rye grass mixture with Hungarian vetch,
based on yield and yield components

est stem diameter, second higher leaf/stem ratio and with oat average yield (28.87 t ha-1). Consequent-
the last one pure Hungarian vetch sowing which is ly all of the investigated parameters in the present
second lowest fresh and dry forage yield, the high- study, 60% or 40% HV mixture with oat combina-
est stem diameter, lower leaf/stem ratio and lower tion can be recommended for high yield forage pro-
plant height, combinations. From these results it can duction.
be evaluate that using yield and five yield compo-
nents, Hungarian vetch mixture with oat combina-
tion at both mixture rate can be successfully recog- ACKNOWLEDGMENTS
nize from vetch mixture with annual rye grass and
pure vetch sowing. This research is a part of the project
NKUBAP.00.24.AR.13.17. The authors express
their gratitude to Tekirdağ Namık Kemal Univer-
CONCLUSION sity for support.

Forage yield is a quantitative trait that is strong-


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