Sharda University

School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma Class : 1 st Year

Subject: P'ceutical Chemistry Practical (ER20-12P)
Exp No. Aim
1 To study the various glassware and appartus used in pharmaceutical chemistry laboratory.
2 To perform the limit test of chloride for the given sample.
3 To perform the limit test of sulphate for the given sample.
4 To perform the limit test of iron for the given sample.
5 To prepare and standardize 0.1N sodium hydroxide solution with oxalic acid.
6 To prepare and standardize 0.1N Potassium permanganate
7 To perform the assay of the given sample of ferrous sulphate by redox titration.
8
To perform the assay of the given sample of Calcium gluconate by complexometry titration.
9 To perform the assay of the given sample of Sodium Chloride by Modified Volhard's
Method.
10 To perform the assay of the given sample of Ascorbic acid by iodometry.
11 To perform the assay of the given sample of Ibuprofen by alkalimetry.
12 To synthesize Benzoic acid from Benzamide and to find out its % practical yield and melting
point.
13
To synthesize Picric acid from Phenol and to find out its % practical yield and melting point.
14
To perform identification test on the given sample of Aspirin as per Indian Pharmacopoeia.
15 To perform identification test on the given sample of Paracetamol as per Indian
Pharmacopoeia.
16 To perform identification test on the given sample of Caffeine as per Indian
Pharmacopoeia.

17 To perform the identification test for cations as per Indian Pharmacopoeia.(Ag +, Pb2+ and
Hg2+)
18 To perform the identification test for anions as per Indian Pharmacopoeia. (Carbonate,
Thiosulphate and chloride ions)
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 1
To study the various glassware and appartus used in
Aim of experiment:
pharmaceutical chemistry laboratory.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers

Chemicals required per

group
Name Grade Quantity
NA

Any other item required per

group
Name Specification Quantity

Name, Sign & Date

 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 2
Aim of experiment: To perform the limit test of chloride for the given sample.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Nessler cylinder 50 ml 2
Measuring Cylinder 25 ml 1
Pipette 5 ml 1

Chemicals required per group

Name Grade Quantity
NaCl 200 mg
AgNO3 2 gm
Dil. HNO3 1 ml

Any other item required per

group
Name Specification Quantity
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 3
Aim of experiment: To perform the limit test of sulphate for the given sample.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Nessler cylinder 50 ml 2
Measuring Cylinder 25 ml 1
Pipette 5 ml 1

Chemicals required per

group
Name Grade Quantity
Ascorbic Acid 2 gm
Dil. Acetic Acid 1 ml
BaCl2 10 gm
Pot. Sulphate 1 gm
Alcohol 20 ml

Any other item required per

group
Name Specification Quantity

Name, Sign & Date

 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 4
Aim of experiment: To perform the limit test of iron for the given sample.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Nessler cylinder 50 ml 2
Measuring Cylinder 25 ml 1
Pipette 5 ml 1

Chemicals required per

group
Name Grade Quantity
0.1N H2SO4
20% Iron free Citric Acid
Thioglycollic acid 10 mL
Ammonia sol"
Ferric ammonium Sulphate 1 gm

Any other item required per

group
Name Specification Quantity
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 5
To prepare and standardize 0.1N sodium hydroxide solution with
Aim of experiment:
oxalic acid.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
NaOH 5 gm
Oxalic acid 1 gm
Phenolphthalein In drops

Any other item required per

group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 6
Aim of experiment: To prepare and standardize 0.1N Potassium permanganate.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
KMnO4 1 gm
H2SO4 5 mL
Oxalic acid 1 gm

Any other item required per

group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 7
To perform the assay of the given sample of ferrous sulphate by
Aim of experiment:
redox titration.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
KMnO4 1 gm
H2SO4 5 mL
Oxalic acid 1 gm
FeSO4 3 gm

Any other item required per

group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 8
To perform the assay of the given sample of Calcium gluconate by
Aim of experiment:
complexometry titration.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
Cal. Gluconate 0.50 gm
MgSO4.7H2O 2 gm
Ammoia sol" 10 ml
disodium EDTA 2 gm
Mordant Black II

Any other item required per

group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 9
To perform the assay of the given sample of Sodium Chloride by
Aim of experiment:
Modified Volhard's Method.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumentric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
Sod. Chloride 300 mg
Nitric acid 15 ml
Nitrobenzene 5 ml
AgNO3 5 gm
Ferric ammonium Sulphate 10 ml
Ammonium thiocynate 5 gm

Any other item required per

group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 10
To perform the assay of the given sample of Ascorbic acid by
Aim of experiment:
iodometry.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
Ascorbic acid 200 mg
Sulphuric acid 5 ml
Iodine 2 gm
Pot. Iodide 4 gm
Starch 1 gm

Any other item required per

group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 11
To perform the assay of the given sample of Ibuprofen by
Aim of experiment:
alkalimetry.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 100 ml 2
Spatula 2
Conical flask 100 ml 1
Burette 50 ml 1
Measuring cylinder 50 ml 1

Chemicals required per

group
Name Grade Quantity
Ibuprofen 500 mg
CH3CH2OH 100 ml
NaOH 500 mg
Phenolphthalein 1%

Any other item required

per group
Name Specification Quantity
Name, Sign & Date
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 12
To synthesize Benzoic acid from Benzamide and to find out its %
Aim of experiment:
practical yield and melting point.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Heating mentle 1
Vaccum pump 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Benzamide 5 gm
Sodium hydroxide 10 gm
HCl 10 ml

Any other item required

per group
Name Specification Quantity

Name, Sign & Date

 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 13
To synthesize Picric acid from Phenol and to find out its %
Aim of experiment:
practical yield and melting point.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Water Bath 1
Vaccum pump 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Phenol 4 gm
Sulphuric acid 5 ml
conc. Nitric acid 15 ml
Alcohol 50 ml

Any other item required

Specification Quantity
per group
Name
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 14
To perform identification test on the given sample of Aspirin as
Aim of experiment:
per Indian Pharmacopoeia.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Water Bath 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Aspirin 500 mg
Sod. Hydroxide 1 gm
Sulphuric acid 10 ml
Ferric chloride 2 ml
Ethanol 5 ml

Any other item required

per group
Name Specification Quantity
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 15
To perform identification test on the given sample of Paracetamol
Aim of experiment:
as per Indian Pharmacopoeia.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Water Bath 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Paracetamol 200 mg
Methanol 100 ml
HCl 2 ml
Pot. Dichromate 1 gm

Any other item required

Specification Quantity
per group
Name
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 16
To perform identification test on the given sample of Caffeine as
Aim of experiment:
per Indian Pharmacopoeia.
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Water Bath 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Caffine 0.1 g
HCl 5 ml
Pot. Chlorate 0.2 g
Tannic acid 2 ml
Iodine solution 2 gm
NaOH 1 gm
KI 1 gm

Any other item required

Specification Quantity
per group
Name
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 17
To perform the identification test for cations as per Indian
Aim of experiment:
Pharmacopoeia.(Ag+, Pb2+ and Hg2+)
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Water Bath 1
Vaccum pump 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Dil. HCl 10 ml
KI 1 gm
NH4OH 1 gm

Any other item required

Specification Quantity
per group
Name
 Sharda University
School of Pharmacy
Plot no 32, 34, Knowledge Park III, Greater Noida, Uttar Pradesh 201310

Programme: D.Pharma
Semester: 1
Practical Subject: P'ceutical Chemistry Practical
Subject Code: ER20-12P
Faculty name: Mr. Ravi Sharma
Experiment No.: 18
To perform the identification test for anions as per Indian
Aim of experiment:
Pharmacopoeia. (Carbonate, Thiosulphate and chloride ions)
Batch Size: 22
No. of Groups: 7

Instruments required
Name Make Numbers
Water Bath 1
Vaccum pump 1

Glassware required per

group
Name Capacity Numbers
Volumwntric flask 100 ml 2
Beaker 250 ml 1
Spatula 1
Beaker 500 ml 1
Reflux condenser 50 ml 1
Measuring cylinder 50 ml 1
RBF 250 ml 1
Funnel

Chemicals required per

group
Name Grade Quantity
Thiosulphate Ion
Carbonate ion
Sulphuric acid
Ca(OH)2
Silver Nitrate
MnO2
HNO3
NH4OH

Any other item required

Specification Quantity
per group
Name