Student name: Nguyễn Thị Phương Hảo

Class: 71K_Group: 4

REPORT
Lesson 3: PLANT TISSUE AND CELL CULTURE AND
INTRODUCTION OF BASIC LABORATORY EQUIPMENT

* Table describing the morphological characteristics of colonies for each type of

microorganisms
Shape Diameter Color Odor
Bacteria Usually in the Usually ranges There are No ordor
form of a in size from many colors:
paste, opaque 0.5 mm to 2 - yellow, blue,
(or clear), dry 3cm pink, brown,
or wet, with a depending on red,...
unique shape the species depending on
for each strain the species
and species
(round,
irregular,
filamentous,
rhizoid,
curled,...)
Fungi Dry, spongy, Size from 1- Different No ordor
dense 2mm to 5 – 10 colors: white,
mycelium cm (depending blue, black,
clearly visible on species) red, yellow,
brown,...
Actinomycetes Lime powder, 1 – 2mm – Different Has a
sometimes in 1,2cm colors: white, characteristic
the shape of green, yellow, earthy smell
radioactive purple,
concentric black,...
circles specific to
each group
and species
Yeasts Paste, usually 3 – 5 Usually seen No ordor
round and micrometers in ivory white
shiny
* Results of counting the total number of CFU/ agar plate
- Hansens: 25
- Gzapeck: 62
- MPA: 112
- Gause I: 69
* The number of CFU/ agar must be > 25 and < 250 because: Counts above 250
are considered too numerous to count and it is difficult as well as time-consuming to
distinguish the individual colonies on a plate because it is impossible to tell whether
colonies are separated. Plates with less than 25 colonies, the count may not be
accurate and do not have a statistically significant number of colonies.
* Results of counting the number of CFU of each type of microorganisms/ agar
plate
- Hansens:
+ Bacteria: 6
+ Fungi: 19
- Gzapeck:
+ Bacteria: 57
+ Fungi: 5
- MPA:
+ Bacteria: 82
+ Fungi: 30
- Gause I:
+ Bacteria: 67
+ Fungi: 2
* Calculate the number of microbial cells of each type/ml of isolated sample
(Waste water; Volume plated: 0,5ml; Dilution factor: 10-5)
- Hansens:
1 1
+ Bacteria: N = 10 x 6 x x −5 = 12000000
0 ,5 10

1 1
+ Fungi: N = 10 x 19 x x −5 = 38000000
0 ,5 10

- Gzapeck:
1 1
+ Bacteria: N = 10 x 57 x x −5 = 114000000
0 ,5 10
 1 1
+ Fungi: N = 10 x 5 x x −5 = 10000000
0 ,5 10

- MPA:
1 1
+ Bacteria: N = 10 x 82 x x −5 = 164000000
0 ,5 10

1 1
+ Fungi: N = 10 x 30 x x −5 = 60000000
0 ,5 10

- Gause I:
1 1
+ Bacteria: N = 10 x 67 x x −5 = 134000000
0 ,5 10

1 1
+ Fungi: N = 10 x 2 x x −5 = 4000000
0 ,5 10

* Calcualte the total number of microbial cells/ ml of initial sample (Waste

water; Volume plated: 0,5ml; Dilution factor: 10-5)
1 1
- Hansens: N = 10 x 25 x x −5 = 50000000
0 ,5 10

1 1
- Gzapeck: N = 10 x 62 x x −5 = 124000000
0 ,5 10

1 1
- MPA: N = 10 x 112 x x −5 = 224000000
0 ,5 10

1 1
- Gause I: N = 10 x 69 x x −5 = 138000000
0 ,5 10