CHEMISTRY CLASS 1ST YEAR CHAPTER#3

Marks: 30 Time Allowed: 1:20hr

1. CHOOSE BEST POSSIBLE ANSWER. (14)

1 An activated enzyme made of polypeptide chain and substrate co-enzymes co-enzymes holoenzyme
a co-factor is:
2 The catalytic activity of an enzyme is restricted to its active site passive site cofactor binding site
small portion:

3 At low concentration of substrate the reaction rate is substrate enzyme cofactor coenzyme
directly proportional to the: available available available available
4 Active site of enzyme is produced by: globular charges fibrous both a and b
structure structure
5 Temp. is going from 15 to 41° C, rate of reaction is down up down then up up then
going: down
6 A complete enzyme is: Holoenzyme Apoenzyme Coenzyme Cofactor
7 A group of enzymes called hydrolases acts upon: Lipids Proteins Carbohydrate All
8 Blocking of active site of an enzyme temporarily is a Non- Irreversible Competitive Feedback
type of…Inhibition: competitive
9 Sucrase (invertase) acts on: Sucrose Sucrose & Any Any organic
starch disaccharide Monomer
10 Energy must be added for a chemical reaction to Activation Entropy Heat Temperature
start. This is energy of:
11 Which one is the essential raw material for Proteins Vitamins Carbohydrate Lipids
coenzymes? s
12 Which of the following will not occur as a result of Enzyme Enzyme Enzyme Enzyme
high temperature on enzymes? changes loses coagulates becomes more
shape solubility effective
13 Which of the following enzymes works best at a pH in Amylase Maltase Lipase Pepsin
the range 2–3?
14 Which of the following is not a characteristic of pH sensitive Heat Required in Irreversibly
enzymes? sensitive large damaged by
quantities the cold
2. ANSWER ANY EIGHT QUESTIONS. (16)

1 Define cofactor? How is it important?

2 What is activation energy? How do enzymes lower it?
3 Differentiate b/w Apoenzyme & Holoenzyme?
4 What is feedback mechanism? Give an example?
5 Enzymes do not float freely always. Comment?
6 What do you infer by Allosteric site?
7 Define saturation & denaturation?
8 Differentiate b/w Coenzyme & Prosthetic group?
9 How does pH affect enzyme activity?
10 Differentiate b/w Reversible & Irreversible inhibitors?